ライフサイエンスコーパス: organotypic culture

1 imensional culture conditions (physiomimetic organotypic culture).

2 eric and submucosal plexuses after 7 days in organotypic culture.

3 Intact otic capsules were maintained in organotypic culture.

4 ture dish than the much more labor-intensive organotypic culture.

5 sponses as modeled in three-dimensional (3D) organotypic culture.

6 uced expression of interleukin-1alpha by the organotypic culture.

7 elium is formed by the NIKS keratinocytes in organotypic culture.

8 helial stratification and differentiation in organotypic culture.

9 rized architecture in three dimensional (3D) organotypic culture.

10 at postnatal days (P7-P14) and maintained in organotypic culture.

11 dence for the defective DNA damage repair in organotypic culture.

12 ted with dominant-negative CaSR in postnatal organotypic cultures.

13 important for binding to p130 in extracts of organotypic cultures.

14 N lesions, condylomata, and HPV16-transduced organotypic cultures.

15 n rat cochlea outer hair cells maintained in organotypic cultures.

16 ulate a normal epidermis, REKs were grown in organotypic cultures.

17 from single basket interneurons in cortical organotypic cultures.

18 n primary visual cortex in vivo and cortical organotypic cultures.

19 were made from SGNs in P0-P5 mouse cochlear organotypic cultures.

20 , brefeldin A was used to block secretion in organotypic cultures.

21 up-regulated following Shh treatment in the organotypic cultures.

22 f II-4 cells was altered by ultraviolet B in organotypic cultures.

23 rin beta1; and increased cell invasion in 3D organotypic cultures.

24 tion and established novel three-dimensional organotypic cultures.

25 abnormality was not detected in neocortical organotypic cultures.

26 liferation, and apoptosis in HaCaT-ras/bcl-2 organotypic cultures.

27 g ultraviolet irradiation in HaCaT-ras/bcl-2 organotypic cultures.

28 increased expression of GLT-1 in spinal cord organotypic cultures.

29 latory region during the viral life cycle in organotypic cultures.

30 pallidosubthalamic projection was studied in organotypic cultures.

31 aintained in either submerged cultures or in organotypic cultures.

32 ammary epithelia in vivo, ex vivo, and in 3D organotypic cultures.

33 h two-colour Ca(2+) and glutamate imaging in organotypic cultures.

34 ld be unlike other herpesviruses examined in organotypic cultures.

35 opment and also upon stimulation in cortical organotypic cultures.

36 drocytes, mixed glial cells, and spinal cord organotypic cultures.

37 ingle epithelial cells and three-dimensional organotypic cultures.

38 role of oxidative stress was investigated in organotypic cultures.

39 ologous components in chimeric monolayer and organotypic cultures.

40 alization of beta-catenin in vitro and in 3D organotypic cultures, accompanied by upregulation of WNT

41 tested in three-dimensional tracheospheres, organotypic cultures, air-liquid interface cultures, and

42 In organotypic culture, AKT mediates changes related to cel

43 ion of M-CSFR on microglia endogenous to the organotypic culture also rescued neurons from excitotoxi

44 In both the 3D organotypic culture and direct mucosal injury models, th

45 Organotypic culture and human tumour explants were allow

46 ally relevant drugs blocked invasion both in organotypic culture and in animals, suggesting a new tre

47 up-regulated in EGFR-overexpressing cells in organotypic culture and in EGFR transgenic mice.

48 lionic axons based on their disappearance in organotypic culture and on their expression of tyrosine

49 l day 6 cerebellar slices were maintained in organotypic culture and treated with glutamate receptor

50 We then developed a cochlear organotypic culture and video-microscopy approach to mon

51 ECs in rigid and compliant three-dimensional organotypic cultures and 2) restores the induction of th

52 69 pairs in cortex-striatum-substantia nigra organotypic cultures and 538 pairs in acute striatal sli

53 We investigated this issue in mature organotypic cultures and acute slices of rat cortex by r

54 use of biomechanically compliant or rigid 3D organotypic cultures and combined them with global micro

55 were recorded from hair cells in denervated organotypic cultures and in acutely excised epithelia.

56 sualize nests of marked, dysplastic cells in organotypic cultures and in cultures transplanted to nud

57 on production has been limited to cumbersome organotypic cultures and mouse xenografts.

58 Muscles of P0 mice were also put into organotypic cultures and treated with a neutralizing Kit

59 ion was significantly inhibited in HaCaT-ras organotypic cultures and was associated with high rates

60 A expression, reduced tumor cell invasion in organotypic culture, and restricted tumor growth in vivo

61 Here, by using NP cells, brain organotypic cultures, and in vivo animal models, we inve

62 orted long-term epidermal regeneration in 3D organotypic cultures, and resulted in the manifestation

63 loss and dendritic regression in hippocampal organotypic cultures, and selective blockade of the CRFR

64 ctors that differed between wild-type and MT organotypic cultures, and then corrected these differenc

65 ian cells lines, as well as mouse cerebellar organotypic cultures, and we also show that they exhibit

66 Furthermore, by using skin organotypic cultures as a model system to monitor the co

67 ion or by growth and maturation in epidermal organotypic culture) as well as a cytokine or bacterial

68 nicotinic activation of alpha7-nAChRs in WT organotypic culture, as well as cell culture, increases

69 differentiation of the epithelium formed in organotypic culture, assessed by basal K19 and suprabasa

70 When cultured alone in organotypic culture, both basal- and luminal-type breast

71 d an invasive phenotype in three-dimensional organotypic cultures but failed to cause HBECs to form t

72 missive for complete epithelial formation in organotypic culture, but imposes a growth constraint in

73 spase-14) were normally expressed in control organotypic cultures, but were expressed at reduced leve

74 e investigated in nonobese diabetic mice and organotypic cultures by immunohistochemistry, microarray

75 resembles that induced in murine spinal cord organotypic cultures by tumor necrosis factor alpha (TNF

76 analysis procedure for structurally complex organotypic cultures (CALYPSO) applied to fluorescence-b

77 cellular P. gingivalis in both monolayer and organotypic cultures compared to free antibiotic or poly

78 TPA (0.001 microg/ml) was then added to organotypic cultures containing mixtures of NHK:II-4 cel

79 For this purpose, organotypic cultures containing the cortex, the striatum

80 RNA interference studies both in vivo and in organotypic culture demonstrate that the beta2*-nAChRs a

81 el quantitative image analysis tool with 3-D organotypic cultures, demonstrate that stromal changes a

82 induced in primary astrocytes, neurons, and organotypic cultures, demonstrating that this phenomenon

83 In this study, we use organotypic cultures derived from transgenic mice induci

84 Organotypic cultures developed normal, tonotopically spe

85 In cell and organotypic cultures, Dkk4 inhibition is eliminated by e

86 When established in organotypic culture, EGFR-overexpressing cells had evide

87 When grown in organotypic culture, EH18-1 cells form multiple layers a

88 In organotypic culture employing dermal and epidermal compa

89 To test this, we exposed organotypic cultures for 3 days to smokeless tobacco aqu

90 and filaggrin/beta-gal of mixtures grown in organotypic cultures for 7 days demonstrated that when i

91 nd rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that a

92 commissure can regenerate across a lesion in organotypic cultures from postnatal day (P) 6 gerbils, b

93 In the present study, we further exploit organotypic cultures from wild-type and SOD1(G93A) mice

94 fected auditory and vestibular hair cells of organotypic cultures generated from the postnatal mouse

95 nputs using cortex-striatum-substantia nigra organotypic cultures grown for 45 +/- 4 d.

96 eurons from cortex-striatum-substantia nigra organotypic cultures grown for 5-6 weeks using somatic w

97 Controlling the microenvironment of such organotypic cultures has impact in tissue engineering, c

98 in authentic functional properties in mature organotypic culture, hippocampal slice cultures were est

99 In organotypic cultures, HPV-11-positive cells exhibited al

100 solated cells of the ICC lineage and gastric organotypic cultures, hyperglycemia stimulated prolifera

101 ange in regenerative capacity can develop in organotypic cultures, IC slices with an intact commissur

102 potential decline and is rescued in cochlear organotypic cultures in low potassium milieu, indicating

103 ardial biopsies were harvested and plated as organotypic cultures in the presence or absence of MSC f

104 agging of endogenous proteins in primary and organotypic cultures in vitro and developing, adult, age

105 , we have generated novel, three-dimensional organotypic cultures in which skin keratinocytes were gr

106 We have adapted organotypic cultures, in which oral gingival keratinocyt

107 ssion of shh in HaCaT keratinocytes grown in organotypic cultures induced a basal cell phenotype, as

108 ells by either methylcellulose suspension or organotypic culture induces LMP1 expression prior to Z a

109 pically along the margin of the hindbrain in organotypic cultures, inferior olivary axons could pathf

110 These putative progenitors were absent from organotypic cultures lacking mature ICC (Kit(+)CD44(+)CD

111 and knockdown of Scrib in lung explants and organotypic cultures leads to reduced cohesion of lung e

112 We here describe that in stable hippocampal organotypic cultures, LTD also is not a unitary event bu

113 elium exists as a stratified tissue in vivo, organotypic cultures may serve as a better model of EBV

114 ous bronchial epithelial cells cultured with organotypic culture methods.

115 that the pattern of GABAergic neurons within organotypic cultures mimicked the expression pattern see

116 e observations illustrate the utility of the organotypic culture model for the investigation of intra

117 X-2 activity, on motor neuron survival in an organotypic culture model of ALS.

118 el tumor-invasive signature obtained from an organotypic culture model of engineered ESCC.

119 ection of S631G variant into a 3-dimensional organotypic culture model of normal esophageal squamous

120 The rat hippocampal organotypic culture model of post-traumatic epilepsy was

121 epithelial tissues using a three-dimensional organotypic culture model of the mammary duct.

122 This 3D organotypic culture model reproduced key features of des

123 in rat keratinocytes, we created an in vitro organotypic culture model that closely mimics the diseas

124 udy inductive mechanisms, a rat keratinocyte organotypic culture model that faithfully mimics HA meta

125 To accomplish this, we have established an organotypic culture model that mimics a preneoplastic ti

126 re mutant (Kv2.1W365C/Y380T: Kv2.1 DN) in an organotypic culture model to manipulate channel expressi

127 A unique three-dimensional (3D) organotypic culture model was established; within a dilu

128 ary rabbit corneal cell culture model and an organotypic culture model were also used.

129 cytes are induced to differentiate in a skin organotypic culture model, both matriptase and prostasin

130 racterized using a clonogenic assay and a 3D organotypic culture model.

131 have all led to valuable clinical insights, organotypic culture models have emerged as tractable sys

132 While organotypic cultures obtained from tadpoles during preme

133 amined the real-time behavior of cells in an organotypic culture of the mammary glandular epithelium.

134 NA-mediated knock down of Ano1 expression in organotypic cultures of Ano1 WT small intestine.

135 xpression of Kv channels were explored using organotypic cultures of brainstem prepared from P9-P12 r

136 In organotypic cultures of cerebellar slices, brain-derived

137 Finally, organotypic cultures of darkly pigmented human keratinoc

138 This protocol details organotypic cultures of developing mouse, monkey and hum

139 In organotypic cultures of developing rat prostates, Shh in

140 endogenous soluble factor was NRG1, we used organotypic cultures of E24 MAM-treated ferret neocortex

141 le of Cux-1 in mammalian kidney development, organotypic cultures of embryonic mouse kidney were incu

142 Using a combination of genetic approaches, organotypic cultures of embryonic pancreata, and genomic

143 We have used organotypic cultures of embryonic rat spinal cord and do

144 e, we demonstrate that EBV is able to infect organotypic cultures of epithelial cells to establish a

145 Addition of either FasL or TNF-alpha to organotypic cultures of fetal rat olfactory epithelium r

146 reported that KA-mediated neuronal damage in organotypic cultures of hippocampal slices was associate

147 Using organotypic cultures of hippocampal slices, we have inve

148 on and reduce proviral genome integration in organotypic cultures of human cervicovaginal tissue.

149 rmaceutical agents and treatment regimens on organotypic cultures of human disease to expedite drug a

150 l of HIV CNS infection that utilizes explant organotypic cultures of human fetal CNS tissue.

151 gulated cyclin E in epithelial tissues using organotypic cultures of human mammary epithelial cells a

152 In organotypic cultures of human vaginal epithelium contain

153 Using organotypic cultures of Madin-Darby canine kidney cells

154 /D5 receptors and L-type calcium channels in organotypic cultures of neonatal striatum.

155 To understand this response, organotypic cultures of nor-mal keratinocytes were expos

156 d (ii) acinar filling upon three-dimensional organotypic cultures of p130Cas-FL or -CT expressing MEC

157 amplification initiates in spinous cells in organotypic cultures of primary human keratinocytes duri

158 e differentiated cells that reenter S phase, organotypic cultures of primary human keratinocytes tran

159 In submerged and organotypic cultures of primary human keratinocytes, the

160 In this report, we employed organotypic cultures of primary human keratinocytes, whi

161 , we examined the viral infection process in organotypic cultures of primary human keratinocytes.

162 ces of cell-free A beta, and then applied to organotypic cultures of rat brain for varying periods of

163 d Ca(2+) signaling in astrocyte processes in organotypic cultures of rat hippocampus.

164 Using organotypic cultures of rat ventral mesencephalon, the e

165 Here, we show that when organotypic cultures of rodent hippocampal slices were t

166 of this clock-regulated pathway by treating organotypic cultures of SCN with botulinum toxin A or dy

167 rrier in Pelo-deficient mice, we made use of organotypic cultures of skin explants from control and m

168 enyl 153 (PCB153) on the proteome of primary organotypic cultures of the mouse mammary gland.

169 n carcinogenesis was assessed using in vitro organotypic cultures of the spontaneously immortalized,

170 In this investigation, we have used organotypic culturing of epithelial cells as a novel in

171 We also found that, in organotypic cultures, olivary axons which had crossed th

172 Addition of IL-6 to brain organotypic cultures or into the cerebral ventricles of

173 l epithelial cells and the application of 3D organotypic culture (OTC), a form of tissue engineering.

174 MT spinal cord organotypic cultures overexpress certain factors and und

175 An organotypic culture preparation of embryonic chicken spi

176 tro analysis of nigrostriatal development in organotypic cultures prepared from LPS-exposed rat fetus

177 In summary, hippocampal organotypic cultures prepared from young adult rats main

178 In organotypic cultures, prevention of ICC loss by insulin

179 of 2.5 microM TTX for 2 weeks to the SON in organotypic culture produced a 73% decrease in the survi

180 5-positive tumor biopsy specimens (plugs) or organotypic cultures (rafts) consisting of ChHV5-positiv

181 The cells could also be grown on organotypic cultures, resulting in further differentiate

182 with morpholino antisense oligos in cochlear organotypic cultures revealed a negative correlation bet

183 ns grown in cortex-striatum-substantia nigra organotypic cultures show morphological and electrophysi

184 Mcl-1 shRNA organotypic cultures showed increased levels of spontane

185 homogenates and of prion-infected cerebellar organotypic cultured slices and decreased the amount of

186 Moreover, ELISA on cerebellar organotypic cultured slices and in vitro conversion assa

187 e of Herpes simplex virus (HSVTK) cerebellar organotypic cultured slices markedly aggravated prion-in

188 Organotypic cultures support the stratification and diff

189 The alginate scaffold-based organotypic culture system is a promising, reliable, and

190 We used an organotypic culture system of human fetal testes explant

191 in particular, we have developed and used an organotypic culture system of mouse fetal gut.

192 Analysis of spheroid invasion in an organotypic culture system revealed that these "trailbla

193 inogenesis as modeled in a three-dimensional organotypic culture system.

194 velopment of the neonatal mouse retina in an organotypic culture system.

195 ccur when vitamin C is added to air-exposed, organotypic culture systems.

196 ed to deposit their own basement membrane in organotypic culture, TGF-beta2 production was reduced.

197 isms, we present a novel breathing brainstem organotypic culture that generates rhythmic neural netwo

198 ting the growth of breast cancer cells in 3D-organotypic cultures that recapitulated pulmonary microe

199 d the effect of ultraviolet B irradiation on organotypic cultures that were fabricated by mixing norm

200 In thymic organotypic cultures, the thymocyte-induced reduction in

201 However, in organotypic cultures, this extended promoter construct f

202 ously reported that exposure of HPV-positive organotypic cultures to benzo[a]pyrene (BaP), a major ca

203 this study, we used isogenic benign mammary organotypic cultures to interrogate the role of mTOR-med

204 In organotypic culture, treated cells showed a pseudostrati

205 ers, retinas from adult mouse were placed in organotypic culture under serum free conditions and allo

206 voltage-sensitive dye changes in hippocampal organotypic cultures undergoing SD were processed via op

207 In organotypic cultures, UVB treatment also resulted in inc

208 , FN enhanced outgrowth in three-dimensional organotypic cultures via a mechanism that is dependent u

209 in the rat suprachiasmatic nucleus (SCN) in organotypic culture was studied by in situ hybridization

210 Neuronal spiking in organotypic cultures was necessary for the proliferation

211 The toxicity of MT spinal cord organotypic cultures was reduced and axonal lengths were

212 Using organotypic culture, we found that the loss of axon rege

213 Using both mammary organoids and 3D organotypic cultures, we show that MMP activity is neces

214 p-states in cortex-striatum-substantia nigra organotypic cultures, we show that the delay between up-

215 In 3D organotypic cultures, we showed that a spatially distinc

216 ICC in organotypic cultures were also studied after immunoneutr

217 pression of differentiation markers in these organotypic cultures were analyzed by using immunohistol

218 Organotypic cultures were established with bovine kerati

219 ine the ligand responsible for this process, organotypic cultures were exposed to untreated HIV, HIV

220 Organotypic cultures were needle-punctured 100 times, in

221 The organotypic cultures were treated with the transcription

222 d at the epithelial-mesenchymal interface of organotypic cultures, where a BM-like structure is forme

223 ion into three-dimensional matrix gels or in organotypic culture, whereas re-expression of siRNA-resi

224 Foreskin biopsies were infected ex vivo in organotypic culture with HIV-1.

225 res, but were expressed at reduced levels in organotypic cultures with downregulated Mcl-1.

226 Treatment of disease mimic organotypic cultures with IL-1 receptor antagonist led t

227 an increased IL1A and treatment of wild-type organotypic cultures with IL1A was sufficient to induce

228 cells) that was grown in three-dimensional, organotypic cultures with intact basement membrane.

229 can be temporally modulated via treatment of organotypic cultures with oxidized glutathione.

230 s and immunohistochemistry and correlated in organotypic cultures with the beta(3)-induced progressio

231 Treatment of UVB-exposed monolayer or organotypic cultures with trichostatin A, a histone deac