ライフサイエンスコーパス: orthophosphate

1 64 h for 90Y-citrate and Te = 255 h for 32P-orthophosphate).

2 from MDA-486 cells labeled in vivo with [32P]orthophosphate.

3 sphate, releasing two molecules of inorganic orthophosphate.

4 from in-line geometry with respect to bound orthophosphate.

5 e and water (or OH-) are in equilibrium with orthophosphate.

6 pyruvate, adenosine triphosphate (ATP), and orthophosphate.

7 was studied by metabolic labeling with (32)P-orthophosphate.

8 eady state exceeded by 3-fold that of [(32)P]orthophosphate.

9 the greatest amount of NaOH-EDTA extractable orthophosphate.

10 Mv1Lu cells) metabolically labeled with [32P]orthophosphate.

11 owed soil contained lowest concentrations of orthophosphate.

12 e cells were metabolically labeled with [32P]orthophosphate.

13 GA and increased resistance to the inhibitor orthophosphate.

14 and a decreased sensitivity to the inhibitor orthophosphate.

15 n45.6 and connexin56, were labeled with [32P]orthophosphate.

16 bolically labeled with 32P-labeled inorganic orthophosphate.

17 vivo labeling of PS120/NHE3V cells with [32P]orthophosphate.

18 hydrolyzed concomitantly to produce ADP and orthophosphate.

19 residue(s) when excised shoots were fed [32P]orthophosphate.

20 y in vivo labelling of these cells with 32 P-orthophosphate.

21 by a nucleophilic water molecule to release orthophosphate.

22 diacylglycerol (DAG), dihydroxyacetone, and orthophosphate.

23 following incubation of cells with [(32)P(i)]orthophosphate.

24 iphosphates to nucleoside monophosphates and orthophosphates.

25 )), which are more soluble and reactive than orthophosphates.

26 lts in protein precipitation, the effects of orthophosphate (0-64 mM) addition to sodium caseinate so

27 mg/L as P) < hexametaphosphate (0.1 mg/L) < orthophosphate (0.3 mg/L).

28 ella bronchiseptica GmhB bound to Mg(2+) and orthophosphate (1.7 A resolution).

29 A resolution), in a complex with Mg(2+) and orthophosphate (1.8 A resolution), and in a complex with

30 hromatography revealed that it was all (32)P-orthophosphate ((32)P(i)).

31 ne-seeking radiopharmaceuticals, such as 32P-orthophosphate, 89Sr-chloride, 186Re-1,1 hydroxyethylide

32 In all experiments with orthophosphate addition of at least 1 mg/L as P, peaking

33 Orthophosphate addition, however, represses mitotic PHO5

34 Orthophosphate and 3'-phosphates were also detected in t

35 he similarity in pain relief afforded by 32P-orthophosphate and 89Sr-chloride, this hypothesis is exa

36 .0020 cGy/h/kBq and 0.0026 cGy/h/kBq for 32P-orthophosphate and 90Y-citrate, respectively.

37 vage of phosphonoacetaldehyde (Pald) to form orthophosphate and acetaldehyde.

38 nophosphate, while ADP is hydrolyzed to form orthophosphate and AMP.

39 vivo by pulse-labeling HeLa cells with [32P]orthophosphate and chasing using three different techniq

40 ADP is attacked by water with liberation of orthophosphate and formation of AMP.

41 At neutral pH, products of the reaction (orthophosphate and fructose 6-phosphate) bind to the act

42 used to determine the binding force between orthophosphate and iron (oxy)hydroxide that was coated o

43 in the raw activated sludge was dominated by orthophosphate and long-chain polyphosphates, whereas in

44 lar techniques and supplied them with (33) P-orthophosphate and O. vulgatum sporophytes with (14) CO2

45 C phosphorylation sites incorporated [(33)P]-orthophosphate and showed a progressive decrease with no

46 proteins, RASM cells were labeled with [32P]orthophosphate and stimulated with 100 nmol/L Ang II for

47 t 2B7 incorporated immunoprecipitable [(33)P]orthophosphate and that 2B7His, previously expressed in

48 egates were governed by the concentration of orthophosphate and the aggregates consisted of all casei

49 Orthophosphate and the oxoanion analogues orthovanadate,

50 ic degradation of long-chain phosphates into orthophosphate and trimetaphosphate whereas heating the

51 The competition between orthophosphate and water-extractable organic matter (WEO

52 Inhibitor blends (Zn+orthophosphate and Zn+NOM+orthophosphate) had stronger i

53 x formed as a result of the reaction between orthophosphates and molybdates ions where ascorbic acid

54 ntly validated through the quantification of orthophosphates and total dissolved phosphorus in pollut

55 -1beta (10 U/ml, 4 h) were labeled with [32P]orthophosphate, and E-selectin was immunoprecipitated us

56 te, hydrocerussite, chloropyromorphite, lead orthophosphate, and lead oxide solids; however, in the p

57 the other hand, AMP, in the presence of GDP, orthophosphate, and Mg(2+), adopts the binding mode of a

58 itivity to its negative allosteric effector, orthophosphate, and more stable interactions between lar

59 rophosphate, and phosphoenolpyruvate to ATP, orthophosphate, and pyruvate and provides diverse functi

60 and water to catalyze the formation of AMP, orthophosphate, and selenophosphate.

61 tosomes were metabolically labeled with [32P]orthophosphate, and solubilized homogenates were subject

62 the significant clinical experience with 32P-orthophosphate, and the similarity in pain relief afford

63 udy, we investigated the mechanistic role of orthophosphate as a corrosion inhibitor in controlling l

64 termine the overall lead exposure when using orthophosphate as a corrosion inhibitor.

65 tic phosphorylation reactions have relied on orthophosphate as the source of phosphorus.

66 very low soil solution concentration of free orthophosphate, as they contain high concentrations of s

67 itates resulted in gradual adsorption of the orthophosphate, causing re-dispersion of the casein-iron

68 phosphates, supports an in vivo role for the orthophosphate complex of Mn(2+) in resistance to oxidat

69 d AGP activity in the presence of a range of orthophosphate concentrations in vitro.

70 es isolated from induced plants had a higher orthophosphate content than granules from noninduced con

71 ing of the other reaction product (inorganic orthophosphate) could not be detected.

72 t run backward synthesizing ATP from ADP and orthophosphate; (d) that its mechanism is a ping-pong on

73 ng of wild-type and mutant B cells with [32P]orthophosphate demonstrated that each of the RFX subunit

74 aortic endothelial cells labeled with [(32)P]orthophosphate demonstrated that only phosphoserine was

75 avage of triphosphate into pyrophosphate and orthophosphate did not occur, indicating that triphospha

76 Increased total organic P and orthophosphate diesters by P NMR and accumulated inosito

77 e surface soils showed higher proportions of orthophosphate diesters under paddy than under non-paddy

78 emperatures with altered amounts of pyruvate orthophosphate dikinase (PPDK) and Rubisco or altered pr

79 mbined with the cytosolic/plastidic pyruvate orthophosphate dikinase (PPDK) catalyze two key steps du

80 horibosyl transferase (APRTase) and pyruvate orthophosphate dikinase (PPDK) convert adenine to ATP fo

81 The enzyme pyruvate,orthophosphate dikinase (PPDK) interconverts pyruvate an

82 Pyruvate orthophosphate dikinase (PPDK) is a critical enzyme for

83 Pyruvate orthophosphate dikinase (PPDK) is a key enzyme in C(4) p

84 Pyruvate, orthophosphate dikinase (PPDK) is a ubiquitous, low-abun

85 RT-DD also detected mRNA for pyruvate, orthophosphate dikinase (PPDK) which has already been sh

86 included the two known isoforms of pyruvate orthophosphate dikinase (PPDK), large and small subunits

87 sphoribosyl transferase (APRT), and pyruvate orthophosphate dikinase (PPDK), respectively.

88 bidopsis, and that the second uses pyruvate, orthophosphate dikinase (PPDK).

89 se in addition to the reduction of pyruvate, orthophosphate dikinase activity in o2 endosperm is comp

90 specific) and the genes that encode pyruvate orthophosphate dikinase and phosphoenolpyruvate carboxyl

91 sferase converts adenine to AMP and pyruvate orthophosphate dikinase converts AMP to ATP.

92 umulation of two isoforms of CA and pyruvate,orthophosphate dikinase in M cells.

93 g phosphoenolpyruvate carboxylase, pyruvate, orthophosphate dikinase, and the 2'-oxoglutarate/malate

94 Both in vivo radiolabeling (using [(32)P]orthophosphate) followed by thin-layer or high-performan

95 Phosphorus, in its orthophosphate form (P(i)), is one of the most limiting

96 Increasing orthophosphate from 0.5 to 1.0 mg L(-1) (as PO4(3-)) acc

97 '-labeling of the adducts by transfer of 32P-orthophosphate from [gamma-32P]ATP mediated by polynucle

98 oups bind irrotationally to bone, displacing orthophosphate from the bone mineral matrix.

99 hosphatase and atypically releases inorganic orthophosphate from triphosphates instead of pyrophospha

100 Gallium orthophosphate (GaPO4) is an alternative piezoelectric m

101 e-dispersed soluble complexes of casein-iron-orthophosphate generated using this process could be use

102 lyphosphate (polyP) is an anionic polymer of orthophosphate groups linked by high energy bonds that t

103 hibitor blends (Zn+orthophosphate and Zn+NOM+orthophosphate) had stronger inhibitory effects compared

104 Addition of orthophosphate has been commonly employed to suppress le

105 Orthophosphate immobilized oxidized lead as insoluble hy

106 iosynthesis of monoselenophosphate, AMP, and orthophosphate in a 1:1:1 ratio from selenide and ATP.

107 iosynthesis of monoselenophosphate, AMP, and orthophosphate in a 1:1:1 ratio from selenide and ATP.

108 is examined in this study using 32P- and 33P-orthophosphate in a mouse femur model.

109 monstrated increased incorporation of [32PO4]orthophosphate in drug-treated cells.

110 resulted in the production of only inorganic orthophosphate in the hydrochar.

111 rporation of 18O from H218O exclusively into orthophosphate in the overall selenide-dependent reactio

112 equivalent labeling of the protein with [32P]orthophosphate in the presence and absence of cyclohexim

113 endent inhibition of immunodetectable [(33)P]orthophosphate in UGTs and protein kinase Cepsilon (PKCe

114 replication by metabolic labeling with [32P]orthophosphate in vivo and obtained direct evidence that

115 P is converted quantitatively to AMP and two orthophosphates in a very slow partial reaction.

116 ar UGT1A7 and UGT1A10 activities and of [33P]orthophosphate incorporation into immunoprecipitable pro

117 n of protein kinase A (PKA) increased [(32)P]orthophosphate incorporation into perilipin 5 by 2-fold,

118 Use of [(32)P]orthophosphate incorporation, pervanadate treatment, and

119 1 followed by metabolic labeling with [(32)P]orthophosphate indicated that p42(mapk/erk2) phosphoryla

120 Metabolic labeling studies using [32P]orthophosphate indicated that the poor prenylation of th

121 f colloidal structures involving casein-iron-orthophosphate interactions.

122 The incorporation of [32P]orthophosphate into immunoprecipitated TTF-1 protein als

123 inase C, also enhanced incorporation of [32P]orthophosphate into TTF-1 protein; however, the DNA bind

124 Measuring orthophosphate is an important tool in biochemical analy

125 ymatic hydrolysis of these esters to produce orthophosphate is often a required reaction preceding ph

126 Free orthophosphate is the form of P taken up by plants, but

127 cies are long-term labeled in vivo with [32P]orthophosphate, isolated in a highly selective way, enzy

128 tes and eukaryotes synthesize long chains of orthophosphate, known as polyphosphate (polyP), which fo

129 By immunoprecipitation carried out on [(32)P]orthophosphate-labeled AtT-20 pituitary cells stably exp

130 First, [(32)P]orthophosphate-labeled cells (Sf9, 3T3-L1 fibroblasts, a

131 Separating tryptic peptides from [(32)P]orthophosphate-labeled cells and analyzing the phosphope

132 Furthermore, in [(32)P]orthophosphate-labeled cells, pantophysin was phosphoryl

133 [32P]Orthophosphate-labeled dUTPase was purified from HeLa ce

134 Phenol-chloroform extraction of [(32)P]orthophosphate-labeled Escherichia coli cells followed b

135 Exposure of [(32)P]orthophosphate-labeled human pulmonary artery endothelia

136 increased in response to TNFalpha in [(32)P]orthophosphate-labeled macrophages, although the level o

137 tion and ODC immunoprecipitation from [(32)P]orthophosphate-labeled NHEK lysates showed that a phosph

138 as confirmed by showing a 2-fold increase in orthophosphate-labeled Sp1 with EGF and okadaic acid.

139 or protein was markedly stimulated when [32P]orthophosphate-labeled Swiss 3T3 cells or CHO-mBR1 cells

140 However, immunoprecipitation of [32P] orthophosphate-labeled UBF from hypertrophying neonatal

141 Immunoprecipitation of [32P]orthophosphate-labeled UBF from hypertrophying, neonatal

142 Orthophosphate labeling and immunoprecipitation of an ep

143 irmed to be phosphorylated in vivo by [(32)P]orthophosphate labeling and peptide mapping.

144 Additionally, orthophosphate labeling coupled with phosphoamino acid a

145 Phosphatase treatment and orthophosphate labeling demonstrated that the species wi

146 In vivo [32P]orthophosphate labeling experiments demonstrated that PP

147 otransfected IRS-1 as demonstrated by [(32)P]orthophosphate labeling experiments.

148 In vivo [32P]orthophosphate labeling indicated that the rescue of the

149 Metabolic [(32)P]orthophosphate labeling of human ARNT-transfected COS-1

150 1) Based on [(32)P]orthophosphate labeling of protein-bound nucleotide, Rab

151 Using orthophosphate labeling we showed a decrease in phosphor

152 Immunoprecipitation studies with [32P]-orthophosphate-labelled cells demonstrated that IncG is

153 radioimmunoprecipitation of lysates of [32P]-orthophosphate-labelled infected HeLa cells with anti-In

154 osphorylation of hERG was measured by [(32)P]orthophosphate labelling of immunoprecipitated protein w

155 n intact neutrophils was confirmed by [(32)P]orthophosphate loading, followed by fMLP stimulation in

156 Using a [32P]orthophosphate metabolic labeling procedure to study HDV

157 consists of an aqueous suspension of silver orthophosphate microparticles under UV illumination, in

158 Polyphosphate (polyP), a polymer of orthophosphate moieties released from the dense granules

159 The effects of orthophosphate, nucleotide analogues, ADP, and covalent

160 After intravenous administration of 32P-orthophosphate or 90Y-citrate in Swiss Webster mice, DNA

161 ubstrate binding was not inhibited by either orthophosphate or glycerol 3-phosphate, indicating that

162 lowing metabolic labeling of cells with [32P]orthophosphate or in vitro in phosphorylation assays wit

163 tory effects compared to each inhibitor (Zn, orthophosphate or NOM) alone, whereas Zn+NOM showed a le

164 sensing protocol allows the determination of orthophosphates over the range from 0.5 to 20 mug L(-1)

165 leaf phosphoenolpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylati

166 , and phosphoenolpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylati

167 ur assay, malachite green is used to measure orthophosphate (P(i)) concentrations after degradation b

168 erichia coli detects environmental inorganic orthophosphate (P(i)) to regulate genes of the phosphate

169 nversion of adenosine 5'-triphosphate (ATP), orthophosphate (P(i)), and pyruvate with adenosine 5'-mo

170 In the presence of inorganic orthophosphate (P(i)), succinyl-CoA, and either GDP, ADP

171 dant complexes with small metabolites (e.g., orthophosphate, peptides), which exhibit narrow EPR sign

172 Pyruvate,orthophosphate (Pi) dikinase (PPDK) is best recognized a

173 ethionine (AdoMet), pyrophosphate (PPi), and orthophosphate (Pi) from ATP and L-methionine.

174 Variation in the concentration of orthophosphate (Pi) in actively contracting, chemically

175 the ability to convert phosphite (Phi) into orthophosphate (Pi) offers an alternative selectable mar

176 However, plants can only acquire inorganic orthophosphate (Pi), meaning global crop production is f

177 dominated blooms to nutrient amendments with orthophosphate (PO4) and inorganic and organic forms of

178 kinesis, we labeled eggs in vivo with [(32)P]orthophosphate, prepared cortices, and mapped LC20 phosp

179 ferred from UDP-N-acetyl[18O]muramate to the orthophosphate produced in the reaction.

180 However, we could document orthophosphate production from ATP catalyzed by the ATP-

181 Additionally, orthophosphate, pyrophosphate and phosphonates were also

182 haracterization of inorganic polyphosphates [orthophosphate, pyrophosphate, tripolyphosphate, trimeta

183 rgy transfer, co-immunoprecipitation, [(32)P]orthophosphate radiolabeling, and measurement of lipolys

184 iron to sodium caseinate solution containing orthophosphate reduced the diffusible phosphorus content

185 Our findings provide insights to how orthophosphate reduces lead levels under drinking water

186 cess releases carbon to the solution whereas orthophosphate remains adsorbed on goethite.

187 d day after injection of 90Y-citrate and 32P-orthophosphate, respectively.

188 dition of iron to caseins in the presence of orthophosphate results in the formation of colloidal str

189 ciated GAPDH by in vivo labeling with [(32)P]orthophosphate revealed the presence of multiple phospho

190 stressed HeLa cells, labeled in vivo by [32P]orthophosphate, revealed four major phosphopeptides A to

191 of epimastigotes labeled for 3 h with (32)P-orthophosphate showed a significant incorporation of the

192 est importance, in vivo labeling with [(32)P]orthophosphate showed that the tryptic phosphopeptide ma

193 egions, with a corresponding increase in the orthophosphate signal, as compared to unhydrolyzed extra

194 The addition of different concentrations of orthophosphate solution to the casein-iron precipitates

195 rea and choline chloride), utilizing various orthophosphate sources.

196 with alanine reduces incorporation of (32)P-orthophosphate substantially.

197 ermeabilized Jurkat T cells using a specific orthophosphate substrate.

198 In the presence of inorganic orthophosphate, succinyl-CoA, and an equimolar amount of

199 of a novel assay procedure for quantitating orthophosphate that is extremely sensitive, reproducible

200 rganic polyphosphates are linear polymers of orthophosphate that modulate blood clotting and inflamma

201 pared with those obtained previously for 32P-orthophosphate, the radiochemical 117mSn(4+)DTPA yields

202 -01 and NIH 3T3 cells were labeled with [32P]orthophosphate to investigate COX phosphorylation in viv

203 lved in these responses, we used radioactive orthophosphate to pulse-label suspension-cultured cells

204 than 600 Da and the reduced binding force of orthophosphate to WEOM-adsorbed iron (oxy)hydroxide AFM

205 in polyphosphate metabolism and V-ATPase in orthophosphate transport were absent from CAP IB HKU-1.

206 e (Omegacalcite = 13), demonstrated that Zn, orthophosphate, tripolyphosphate, and hexametaphosphate

207 Darby bovine kidney cells, labeled with [32P]orthophosphate under normal culture conditions.

208 ine phosphorus excretion, and reduced [(33)P]orthophosphate uptake in rats.

209 complete digestion of the oligomer sample to orthophosphate using acid at high temperature and subseq

210 , all forms of phosphorus are converted into orthophosphates via sample digestion (heating and acidif

211 ssed in transfected cells labeled with [32P] orthophosphate was phosphorylated following the addition

212 th chloramine, with intermittent flow, or if orthophosphate was present.

213 pH buffer with strong complexing properties (orthophosphate) was employed in the background electroly

214 A model distribution system, dosed with orthophosphate, was used to evaluate the effect of corro

215 32P- and 33P-orthophosphate were administered intravenously, and GM-C

216 he nucleus incorporates radiolabel from [32P]orthophosphate whereas cytoplasmic VP22 does not.

217 totic activation is repressed by addition of orthophosphate, which significantly increases cellular p

218 f divalent cations, fructose 6-phosphate and orthophosphate, which together stabilize an R-state conf

219 This study explored the interactions of orthophosphate with casein-iron precipitates.

220 )] which is achieved by activation of [(32)P]orthophosphate with ethyl isocyanate followed by aminoly

221 The interactions of added orthophosphate with iron in the presence and absence of

222 W complexes with nitrogenous metabolites and orthophosphate, with negligible EPR signal from Mn(2+) o

223 o sodium caseinate solution containing 32 mM orthophosphate without any protein precipitation.

224 titation of radiolabeled ATP and radioactive orthophosphate without the generation of large quantitie