ライフサイエンスコーパス: outside-out

1 levated NP(o) of unitary calcium currents in outside-out and cell-attached patches, and elevated calc

2 ion of caffeine or noradrenaline and in both outside-out and inside-out patches when the internal pat

3 ique (cell-attached, inside-out, whole-cell, outside-out and perforated patch) can be achieved, even

4 ced Cl- current (IGABA) was quantified using outside-out and whole-cell patch-clamp recordings beginn

5 r configurations (cell-attached, inside-out, outside-out, and whole-cell).

6 used to test this hypothesis in whole-cell, outside-out, cell-attached, and inside-out patches from

7 on (DRG) neurons were investigated using the outside-out configuration of patch-clamp technique.

8 Using the outside-out configuration of the patch clamp, we elicite

9 d the patch clamp recording technique in the outside-out configuration to investigate, at the single

10 Whole-cell and outside-out configurations of the patch clamp technique

11 oxinin and picrotin inhibited glycine-evoked outside-out currents, picrotin had a 30 times higher IC5

12 Dual somato-dendritic recording, outside-out dendritic recordings, and focal application

13 eceptors expressed in Xenopus oocytes, using outside-out excised patch recording.

14 ely dissociated preparation and a perforated outside-out macropatch of dendritic membrane.

15 LTD, produced in a perforated outside-out macropatch of Purkinje neuron dendrite, whic

16 R334C-CFTR channels in outside-out macropatches activated by ATP alone were mod

17 ent outward potassium current was studied in outside-out macropatches excised from the soma of CA1 py

18 Shaker K+ channels were expressed in outside-out macropatches excised from Xenopus oocytes, a

19 roM) in the presence of 10 microM glycine to outside-out macropatches resulted in openings with an av

20 the modification of R334C-CFTR, measured in outside-out macropatches using a rapid perfusion system,

21 and perforated patch whole-cell and excised outside-out membrane patch recording configurations.

22 ans of a piezoelectric translator to excised outside-out membrane patches allows concentration jumps

23 Brief (2-3 ms) applications of 1 mM GABA to outside-out membrane patches containing alpha1beta3, alp

24 ethyl-lactam potentiates currents in excised outside-out membrane patches elicited by the prolonged a

25 ents were resolved by pulses of glutamate to outside-out membrane patches from Purkinje cells.

26 n of BK-type Ca(2+)-activated K+ channels in outside-out membrane patches from rat olfactory bulb gra

27 Single-channel recordings were made from outside-out membrane patches of Xenopus oocytes injected

28 In outside-out membrane patches, co-application of ACh (4 m

29 In excised outside-out membrane patches, the effects of light on NM

30 brief pulses (1-10 ms) of 1 mM glutamate to outside-out membrane patches, we observed a low-conducta

31 Using excised outside-out membrane patches, we studied the mechanism b

32 duced by application of glutamate or NMDA to outside-out membrane patches.

33 lower when the drug is applied externally to outside-out membrane patches.

34 potency, unitary I(AC) currents recorded in outside-out membrane patches.

35 f single-channel and macroscopic currents in outside-out membrane patches.

36 be resolved in response to ATP (3 microM) in outside-out membrane patches.

37 robability of Kv channel opening in excised, outside-out membrane patches.

38 evoked by brief applications of glutamate to outside-out membrane patches.

39 Channels in patches excised in outside-out mode were blocked by 1 mM Ba2+ but were unaf

40 ell and a granule cell in the whole cell and outside-out modes, respectively, depolarizations of Berg

41 otein in proteoliposomes containing hSMVT in outside-out orientation yielded a catalytic turnover num

42 Second, simultaneous outside-out patch and whole-cell recordings reveal that

43 hole-cell recordings from HEK 293 cells, and outside-out patch clamp recordings from both cell types.

44 Outside-out patch clamp recordings revealed that the max

45 um currents, recorded in both whole-cell and outside-out patch configurations, demonstrated a selecti

46 fluorescent protein in growing axons, and an outside-out patch from mature neuronal membranes that co

47 these channels was assessed in whole-cell or outside-out patch recording as the degree of inward rect

48 We used outside-out patch recording of Xenopus alpha1beta3 Na/K

49 Outside-out patch recordings confirmed this segregation.

50 Outside-out patch recordings demonstrated that the ampli

51 Outside-out patch recordings from cerebellar Purkinje ce

52 binding (the first latency) was estimated in outside-out patch recordings from rat hippocampal neuron

53 Whole-cell recordings and outside-out patch recordings from TE671 cells were made

54 Whole-cell and outside-out patch recordings in slices from bacterial ar

55 By combining outside-out patch recordings of BG AMPA receptors and qu

56 Outside-out patch recordings revealed identical unitary

57 ependent potassium current was studied using outside-out patch recordings with rapid application of d

58 application of GABA + 3alpha5alphaP, and in outside-out patch recordings, suggesting that steroid di

59 its and the kinetic properties examined with outside-out patch recordings.

60 of how fast solutions can be exchanged on an outside-out patch using a dual stream switcher.

61 Following tetanic stimulation an outside-out patch was excised from the apical dendrite n

62 s in response to ATP (whole cell and excised outside-out patch) showed that all formed functional cha

63 ucleus (MNV) of the rat using whole-cell and outside-out patch-clamp recording in coronal brainstem s

64 Using whole-cell and outside-out patch-clamp recordings from granule cells in

65 postnatal development, using whole-cell and outside-out patch-clamp recordings in acute cerebellar s

66 Instead, using outside-out patch-clamp recordings, we demonstrate that

67 is and detected by the use of whole-cell and outside-out patch-clamp techniques on freshly dissociate

68 Moreover, the consistent blockade seen in outside out patches might be ascribed to the confinement

69 and concentration jump techniques applied to outside out patches to evaluate the impact of these muta

70 gonist concentration jumps were performed on outside out patches with multiple NR1/NR2C channels, whi

71 When applied to GABA(A) receptor traces (outside out patches, alpha 1 beta 2 gamma 2S, 1 mM GABA,

72 receptor response to 100 microM serotonin in outside-out patches (n = 19) and whole cells (n = 41) de

73 modelling of currents from cell-attached and outside-out patches (where the number of channels in the

74 y 3 ms, 30 mM) applied to mutant channels in outside-out patches activated currents with a slower ris

75 excitatory postsynaptic conductance, we used outside-out patches and a fast application system to cha

76 single GABA channels from cell-attached and outside-out patches and also introduced some of the prel

77 pionic acid (AMPA) receptors was examined in outside-out patches and at glutamatergic synapses in neu

78 ophysiological measurements on nucleated and outside-out patches and in the whole-cell mode also yiel

79 ificantly reduced the A-type K(+) current in outside-out patches and nearly eliminated the distance-d

80 his agent was applied externally to cells or outside-out patches at concentrations of 5 to 10 microM.

81 diated currents were also studied in somatic outside-out patches at P13-P15 with fast application of

82 ition of acetylcholine-activated currents in outside-out patches by (+)-tubocurarine, pancuronium and

83 ng, we recorded single-channel currents from outside-out patches containing a single active NR1/NR2C

84 apidity to mediate prejunctional depression, outside-out patches containing both ATP-gated and ACh-ga

85 Rapid applications of glutamate on outside-out patches containing NR1a/NR2D(T692A) receptor

86 s studied using ultra-fast drug perfusion of outside-out patches containing rat GluR-A or GluR6 subun

87 In isolated outside-out patches CPA did not evoke SOCs but noradrena

88 Recordings from outside-out patches demonstrated that inward rectificati

89 Outside-out patches displayed two distinct patterns of s

90 econd applications of 1 mM GABA to nucleated outside-out patches elicited rapidly rising biexponentia

91 Fast application of GABA (10 mM) onto outside-out patches excised from bipolar cell terminals

92 Outside-out patches excised from cells transfected with

93 Outside-out patches excised from epsilon subunit-contain

94 applications of GABA (1 mM) to nucleated and outside-out patches excised from granule neurons in cere

95 Single-channel activities were observed in outside-out patches excised from oocytes expressing a ma

96 by using rapid applications of glutamate to outside-out patches excised from Purkinje neurons.

97 (A) receptor antagonist-sensitive current in outside-out patches excised from RGCs, indicating that a

98 NMDA currents recorded from outside-out patches excised from the distal dendrites of

99 In outside-out patches excised from the nonsynaptic face of

100 d using rapid applications of L-glutamate to outside-out patches excised from transfected human embry

101 nel number times open probability (nP(o)) in outside-out patches excised from Xenopus oocytes, with n

102 by their excitatory transmitter, we examined outside-out patches exposed to glutamate.

103 Using outside-out patches formed <or=250 microm away from the

104 educed gamma of 5-HT(3A)(R436C) receptors in outside-out patches from 7.8 +/- 0.5 to 5.0 +/- 0.5 pico

105 brief (1 ms) application of 5-10 mm GABA to outside-out patches from acutely isolated CA1 hippocampa

106 Transporter currents in outside-out patches from astrocytes have faster kinetics

107 perties and distributions of ion channels in outside-out patches from axons and somata of layer 5 pyr

108 tors expressed in Xenopus laevis oocytes and outside-out patches from BOSC 23 cells.

109 Outside-out patches from both cell types under both trea

110 ACh receptors (nAChRs) were investigated in outside-out patches from CA1 stratum radiatum interneuro

111 We find that application of L-glutamate to outside-out patches from cerebellar Bergmann glia activa

112 In outside-out patches from CG neurones containing a 38 pS

113 In outside-out patches from CG neurones, the K+ channel wit

114 Rapid application of 10-300 microM NMDA to outside-out patches from cultured cortical neurons evoke

115 functional binding sites on AMPA channels on outside-out patches from cultured hippocampal neurons.

116 NMDA-activated patch current were studied in outside-out patches from cultured rat cortical neurons.

117 Outside-out patches from Golgi cells exhibited a populat

118 ysed using concentration-jump techniques and outside-out patches from HEK 293 cells.

119 d also activated TRPV4 currents in cell-free outside-out patches from HEK293T cells overexpressing TR

120 Here, in outside-out patches from heterologous cells stably expre

121 es of P2X ATP receptors were investigated in outside-out patches from hippocampal granule cells in br

122 ity of expressed alpha1beta1gamma2 GABARs in outside-out patches from human embryonic kidney 293 cell

123 e to the rapid application of l-glutamate to outside-out patches from human embryonic kidney cells ex

124 Furthermore, D-aspartate-induced currents in outside-out patches from IPCs exhibited larger steady-st

125 ed under hyposmolar recording conditions for outside-out patches from L/M interneurons; no changes we

126 Voltage clamp of outside-out patches from L2/3 neurons revealed that the

127 rations, the open probability of channels in outside-out patches from migrating cells was very high,

128 and kainate receptors show this behavior in outside-out patches from neurons in situ by measuring co

129 Voltage-clamp recordings from outside-out patches from NTS neurones revealed an outwar

130 annel kainate-type currents were observed in outside-out patches from proliferating granule cells in

131 we recorded TTX-sensitive sodium currents in outside-out patches from Purkinje cells acutely isolated

132 Two inactivating currents were present in outside-out patches from pyramidal cells: a rapidly inac

133 ansporter currents were also not detected in outside-out patches from pyramidal neurons.

134 tive cation channel currents were studied in outside-out patches from rabbit portal vein smooth muscl

135 ated channels (10 +/- 1 pS) in inside-out or outside-out patches from rat cultured hippocampal neuron

136 ock of NMDA receptors was studied in excised outside-out patches from rat hippocampal neurons and Xen

137 GABA(A) receptor agonists and antagonists in outside-out patches from rat hippocampal neurons.

138 d, anion-potentiated transporter currents in outside-out patches from these cells exhibited larger am

139 We rapidly applied acetylcholine to outside-out patches from transfected BOSC23 cells and me

140 y 80% of the mechanically induced current of outside-out patches from transfected HEK293 cells.

141 ) glutamate receptor channels was studied in outside-out patches from transiently transfected HEK 293

142 Rs that gave inwardly rectifying currents in outside-out patches from TTX-treated cells was six times

143 iflumic acid (NFA) in excised inside-out and outside-out patches from Xenopus oocytes.

144 Outside-out patches gave a much higher success rate, but

145 ntaneous single-channel events recorded from outside-out patches had the same chord conductance as GA

146 In whole-cell and somatic outside-out patches I(A) activated at -60 mV (half-activ

147 by rapid applications of GABA onto nucleated outside-out patches in cultured postnatal rat hippocampa

148 With isolated outside-out patches Ins(1,4,5)P3 markedly increased the

149 Pulses of ACh were applied to outside-out patches of cell membrane by means of a fast

150 In single-channel recordings from excised outside-out patches of cortical neurons, ethanol inhibit

151 methyl-D-aspartate (NMDA) were recorded from outside-out patches of cultured rat cortical neurons in

152 Agonist pulses applied to outside-out patches of cultured rat hippocampal neurons

153 BA-activated ion channels were recorded from outside-out patches of granule cell bodies.

154 es P(OPEN) in single-channel recordings from outside-out patches of HEK 293 cells.

155 nown superfamilies of NGICs in fast-perfused outside-out patches of membrane.

156 Using outside-out patches of mPFC pyramidal neurons, which pre

157 Outside-out patches pulled from PKD1(115-226)-expressing

158 with voltage clamp, we applied glutamate to outside-out patches pulled from transiently transfected

159 Focally applied GABA and glycine onto outside-out patches revealed that the GABAergic to glyci

160 High-resolution recordings from outside-out patches showed that in all Kir2 channels cur

161 ps (1 ms) of 10 mM glutamate were applied to outside-out patches so that a comparison between the mac

162 In about 45 % of isolated outside-out patches spontaneous channel currents with a

163 Recordings from outside-out patches that contain a single active channel

164 n techniques were used to apply glutamate to outside-out patches that contained GluK1, GluK1/5, or Gl

165 e of open and closed durations recorded from outside-out patches that contained one active NR1/NR2C c

166 Here we report data from outside-out patches that indicate GluR1-containing recep

167 In outside-out patches there was spontaneous cation channel

168 NPo) of the NMDA receptor channel in excised outside-out patches was attenuated by 3HPG but not by 4C

169 Outside-out patches were equilibrated with cisatracurium

170 l currents caused by ACh and ATP in the same outside-out patches were less than additive (85 +/- 10 %

171 Channels in excised outside-out patches were modulated by ligand, indicating

172 cations of GABA (from 10 microM to 10 mM) to outside-out patches were used to study the role that the

173 e ASICs show extremely rapid deactivation in outside-out patches when jumping from a pH 5 stimulus to

174 In outside-out patches with 250 mM external NaCl, the singl

175 2+ inhibited spontaneous channel activity in outside-out patches with IC50 values of 6.8 microm, 25 n

176 inhibited single-channel currents in excised outside-out patches without significantly changing mean

177 in wild-type mouse cells (observed in 50% of outside-out patches), but never observed in mdx cells.

178 d beta-BuTX, unitary currents were recorded (outside-out patches, -60 or -50 mV) that were smaller th

179 In outside-out patches, 11,12-EET also increased the KCa ch

180 With outside-out patches, a transient early suppression of th

181 used membrane breakdown; however, in excised outside-out patches, Ag NPs activated Gd(3+) -sensitive

182 annel open probability determined in excised outside-out patches, but has no effect on single-channel

183 In both inside-out and outside-out patches, channel activity was depressed and

184 the off rate was nearly identical to that of outside-out patches, differences were observed for the o

185 In outside-out patches, ethylbromide tamoxifen increased BK

186 In outside-out patches, GTP was present in the pipette and

187 In outside-out patches, just as desensitized states prolong

188 In outside-out patches, nicotine activated brief 60- and 80

189 In nucleated outside-out patches, PS depressed peak currents and spee

190 In outside-out patches, QA ions in which ethyl groups of TE

191 In excised outside-out patches, the addition of dibutyryl cAMP cons

192 In outside-out patches, the TASK-like background K+ channel

193 In outside-out patches, the type I channel had an almost li

194 Under voltage-clamp conditions in outside-out patches, this crosslink was reduced by DTT a

195 In outside-out patches, troglitazone inhibited KATP channel

196 recordings were made of calcium channels in outside-out patches, under conditions which favoured the

197 In outside-out patches, unedited homomeric GluR6(Q) recepto

198 1 ms pulses of high concentration glycine to outside-out patches.

199 ck by Mg(2+)(i) similar in cell-attached and outside-out patches.

200 examined using cell-attached, inside-out and outside-out patches.

201 aleimide, and could be elicited in cell-free outside-out patches.

202 homomeric GluR1(flip) receptors recorded in outside-out patches.

203 annel activity of Kir2.3 currents in excised outside-out patches.

204 currents of the mechanical channel TREK-1 on outside-out patches.

205 cts on IPSCs with GABAA channel responses in outside-out patches.

206 nels and 15-45 pS TRPC6 channels in the same outside-out patches.

207 ATP activated single channel currents in outside-out patches.

208 nm Ang II induced TRPC6 channel activity in outside-out patches.

209 hannel activity evoked by 1-100 nm Ang II in outside-out patches.

210 tenuated I(A) in both whole-cell and somatic outside-out patches.

211 nts elicited by rapid glycine application to outside-out patches.

212 patches but failed to do so in inside-out or outside-out patches.

213 pulses, and high-frequency trains of GABA to outside-out patches.

214 ties of individual NMDA receptor channels in outside-out patches.

215 ution exchange to study receptor kinetics in outside-out patches.

216 steady-state currents from cell-attached and outside-out patches.

217 , when 5 microm ruthenium red was applied to outside-out patches.

218 channel openings in any capsaicin-sensitive outside-out patches.

219 microcystin also stimulated SOCs in isolated outside-out patches.

220 perties of the 5-HT(3)R were investigated in outside-out patches.

221 que in cell-attached and excised (inside-out/outside-out) patches from embryonic rat dorsal root gang

222 ugh single channels were studied in excised (outside-out) patches.

223 MDARs and alpha7 nAChRs, and recordings from outside-out pulled patches of enlarged presynaptic bouto

224 channels was investigated in parallel using outside-out single channel recording.

225 Outside-out sniffer patches pulled from neurons in the a