ライフサイエンスコーパス: overhang

1 es of the strand terminating with a 5'-ssDNA overhang.

2 p decreases with increasing length of the 5'-overhang.

3 le to the full stretch of 3' human telomeric overhang.

4 ion of the correct single-stranded telomeric overhang.

5 hange (DeltaR(ct)) is found by extending the overhang.

6 ction of free DNA ends to produce a 3'-ssDNA overhang.

7 nd nasally, frequently with Bruch's membrane overhang.

8 same duplex with a 3'-overhang or without an overhang.

9 and to form 5' mononucleotides and a long 3' overhang.

10 ociation of these proteins from the telomere overhang.

11 another by the absence or presence of a stem overhang.

12 ibutes to the generation/maintenance of this overhang.

13 res terminate in a single-stranded 3' G-rich overhang.

14 e leader-proximal repeat to the prespacer 3' overhang.

15 e interaction between POT1 and the telomeric overhang.

16 ferentiates pre-miRNA species with different overhangs.

17 similarities to hairpins and fixed 5' and 3' overhangs.

18 roduce double-stranded DNA molecules with 5'-overhangs.

19 ds unexpectedly form long 5' single-stranded overhangs.

20 m all dsDNA ends: 5'-overhangs, blunt, or 3'-overhangs.

21 wild-type Metnase effectively cleaves ssDNA overhangs.

22 n which DSB ends are converted into 3'-ssDNA overhangs.

23 es in the enzyme that can generate longer 3' overhangs.

24 es out endonucleolytic cleavage of 5' and 3' overhangs.

25 st G to produce fragments with three-base 5'-overhangs.

26 n of DSBs to generate 3' single-stranded DNA overhangs.

27 and from DNA duplexes with single base pair overhangs.

28 define the steps in the processing of these overhangs.

29 RNA constructs, which retained 5' nucleotide overhangs.

30 d inhibiting unwinding of substrates with 3'-overhangs.

31 ike IDN2 and SGS3, FDM1 binds dsRNAs with 5' overhangs.

32 activity mediated loading of Exo1 onto ssDNA overhangs.

33 es with sufficiently long single-stranded 5' overhangs.

34 R mechanisms that rely on 3' single-stranded overhangs.

35 300 nt of G-rich single-stranded DNA (ssDNA) overhangs.

36 sed by lambda exonuclease, leaving behind 3' overhangs.

37 han with single- or double-stranded DNA with overhangs.

38 duplexes with 5'- and 3'-single-stranded DNA overhangs.

39 r to create classic 19-bp siRNAs with 3'-end overhangs.

40 -base-pair prespacer bearing 4-nucleotide 3' overhangs.

41 RNA duplexes, and for duplexes with short 3' overhangs.

42 ends, including blunt, 5' overhangs, and 3' overhangs.

43 o the target DNAs with different lengths and overhangs.

44 Apollo is required for maintaining telomeric overhangs.

45 a similar substrate containing 5-nucleotide overhangs.

46 ctures compared with linear duplex having 3' overhangs.

47 mulate Artemis activity at hairpins or at 5' overhangs.

48 -POT1 switch on telomeric single-stranded 3' overhangs.

49 ficant unwinding of substrates containing 3' overhangs.

50 3-fold lower compared with duplexes with 5' overhangs.

51 commonly used 19mer with two deoxythymidine overhangs (19merTT) variant performed similarly to canon

52 reaction (PCR) fragment containing a single overhanging 3' A to a plasmid vector containing a 3' T.

53 In contrast, 3'-overhang/3'-overhang and 5'-overhang/5'-overhang templat

54 Finally, dephosphorylation of the 5'-overhang/3'-overhang template reduced the efficiency of

55 In contrast, 3'-overhang/3'-overhang and 5'-overhang/5'-overhang templates were processed by resecti

56 blunt/3'-overhang, blunt/5'-overhang, and 3'-overhang/5'-overhang were predominantly repaired with fi

57 breaks (DSBs) providing single-stranded DNA overhangs after their processing.

58 tranded telomeric DNA (ssTEL) and protects G overhangs against RPA binding.

59 In contrast, 3'-overhang/3'-overhang and 5'-overhang/5'-overhang templates were proc

60 ing the detailed structure of the telomere G-overhang and its maintenance will contribute greatly to

61 M may involve double-stranded RNAs with a 5' overhang and the partnering between RDM12 and RDR2.

62 covalent complex containing a single-strand overhang and then compared them with the same overhang d

63 fork substrates with 3' single-stranded DNA overhangs and also disrupts protein-DNA interactions whi

64 in maintaining newly replicated telomeric 3' overhangs and facilitating the switch from replication p

65 ) binds with nanomolar affinity to the ssDNA overhangs and forms a dimer with another telomere-end bi

66 by promoting unwinding of substrates with 5'-overhangs and inhibiting unwinding of substrates with 3'

67 (TERT)] in an effort to extend chromosomal G-overhangs and maintain telomere ends.

68 including human telomeric GQ (with different overhangs and polarities) and GQ formed by thrombin-bind

69 ates, this protein preferentially cleaved 3'-overhangs and RNA in blunt-ended DNA/RNA duplexes.

70 the telomeric C strand, causing extended 3' overhangs and stochastic telomere truncations that could

71 iRNAs by recognizing the presence of 2-nt 3' overhangs and the thermodynamic properties of 2-4 bp on

72 ISPR spacers through the formation of 3'-DNA overhangs and to the degradation of foreign DNA.

73 atible ends with blunt/3'-overhang, blunt/5'-overhang, and 3'-overhang/5'-overhang were predominantly

74 of varying the length of the single-stranded overhang, and studied A-form DNA-PNA duplexes to provide

75 rious types of DNA ends, including blunt, 5' overhangs, and 3' overhangs.

76 substantially shortened, harbored extended G-overhangs, and engaged in end-to-end fusions.

77 elicases, JFH-1 NS3 does not require long 3' overhangs, and it unwinds duplexes that are flanked by o

78 e nucleolytic resection of DNA with 5'-ssDNA overhangs, and that RecQ helicase can initiate resection

79 The overhang annealing is supposed to form circular plasmids

80 high-resolution insight into the telomeric G-overhang architecture under essentially physiological co

81 Telomere overhangs are essential for telomere end protection and

82 n and telomerase extension, but how telomere overhangs are generated is unknown.

83 Telomeric G-overhangs are required for the formation of the protecti

84 the repeat unit and produce a duplex with 5'-overhangs, are extended using a thermostable archaeal DN

85 p formation does not require a single strand overhang, arguing that both terminal strands insert into

86 d double-stranded DNA that had a 3'-poly(dT) overhang as compared with double-stranded DNA with a 5'-

87 lity of gene 6 protein to remove 5'-terminal overhangs as well as to remove nucleotides from the 5'-t

88 s with the binding stability of BLM to ssDNA overhang, as modulated by the nucleotide state, ionic co

89 '-overhanging termini, but not those with 3' overhangs, as found on miRNA precursors.

90 assessment of the rate of formation of each overhang at each nucleotide position.

91 subunit TRF2, initiates formation of the 3' overhang at leading-, but not lagging-end telomeres.

92 -stranded siRNA with at least a 2-nucleotide overhang at one 3' terminus in a dose-dependent manner,

93 a complementary sequence (12-nt) to a G-rich overhang at the 3'-end.

94 assays revealed the presence of a 5'-C-rich overhang at the telomeres of human and mouse chromosomes

95 olled process, ensuring functional telomeric overhangs at chromosome ends.

96 G-overhangs at lagging telomeres are lengthened in S phase

97 of C-strand fill-in, whereas the sizes of G-overhangs at leading telomeres remain stable throughout

98 ollo in the generation of 3' single-stranded overhangs at newly replicated leading-strand telomeres t

99 ned by the generation of 3 single-strand DNA overhangs at the break that are initiated by the action

100 Recent evidence for 5'-cytosine (C)-rich overhangs at the telomeres of the nematode Caenorhabditi

101 We present an overhang-based DNA block shuffling method to create a cu

102 lease activity on single-stranded DNA and 5'-overhangs, because this 5'-exonuclease is not dependent

103 This was the G-overhang binding-protein Pot1a.

104 two adjacently positioned pockets: a 2 nt 3'-overhang-binding pocket within the PAZ domain (3' pocket

105 Moreover, non-compatible ends with blunt/3'-overhang, blunt/5'-overhang, and 3'-overhang/5'-overhang

106 combinational repair from all dsDNA ends: 5'-overhangs, blunt, or 3'-overhangs.

107 inds forked dsDNA and DNA duplexes with a 3'-overhang but is inactive on blunt-ended dsDNA and 5'-ove

108 bstrates possessing a 3' single-stranded DNA overhang but not of 5' overhangs or blunt-ended DNA frag

109 and resection required the presence of these overhangs but did not require Metnase's DNA cleavage act

110 haped DNA structures having >/=18-nucleotide overhangs but not to a similar substrate containing 5-nu

111 s containing 5' overhangs relative to the 3' overhangs but not to blunt-ended duplex.

112 terminal phosphate and a two-nucleotide, 3' overhang, but does not require ATP.

113 Without ATP, dmDcr-2 binds 3' overhanging, but not blunt, termini.

114 ty, particularly for ends with mismatched 3' overhangs, but the mechanism has remained obscure.

115 V alone can stimulate Artemis activity on 3' overhangs, but this DNA-PKcs-independent endonuclease ac

116 res the generation of a 3' single-strand DNA overhang by exonuclease activities in a process called D

117 resection of DNA with blunt-ends or 3'-ssDNA overhangs by DNA unwinding.

118 preferentially inhibits cNHEJ at breaks with overhangs by protecting them.

119 se of subsurface pressure or via creation of overhangs by sublimation, may be a major mass loss proce

120 Here, we show that G-overhangs can undergo cell cycle-regulated changes indep

121 core, oscillating between the two equivalent overhanging carbonyl groups, coupled to an intermolecula

122 PbOAc exchanging between the two equivalent overhanging carboxylate groups (N-core(up) left arrow ov

123 tion in a bis-strapped porphyrin ligand with overhanging carboxylate groups has been investigated in

124 A bis-strap porphyrin ligand (1), with an overhanging carboxylic acid group on each side of the ma

125 omplex, larger DNA sequence that contains an overhang complementary to the CNA can also be encapsulat

126 ble-stranded DNA with nonpairing (nonsticky) overhangs, confined between two-dimensional (2D) lipid b

127 Artemis at blunt DNA ends is slower than at overhangs, consistent with a requirement for a slow DNA

128 self-complementary 14-mer RNAs (12-bp + 2-nt overhang) containing 2(')-OCH(3) and 2'-OH at their 3' e

129 The overhangs corresponded to 2',3'-cyclic phosphate, 3'-pho

130 ALT cells possess excessively long telomeric overhangs derived from telomere elongation processes tha

131 state, ionic conditions, overhang length and overhang directionality.

132 At native telomeres in dna2 mutants, GT-overhangs do clearly elongate during late S phase but ar

133 RNAs with 5' overhangs does not compete with DNA for binding by FDM1,

134 ((SUB)P) is unusually narrow because of the overhanging +domain.

135 lated nucleotide additions during NHEJ of 5'-overhang DSBs or in clastogen resistance.

136 DSBs affects NHEJ, we made site-specific 5'-overhanging DSBs (5' DSBs) in yeast using an optimized z

137 verhang and then compared them with the same overhang duplex in the absence of vTopo.

138 Depletion of TEN1 does not effect overhang elongation in mid-S phase, but it delays overha

139 in 5' TTAGGG 3', while a small portion of G-overhangs end in 5' TAGGGT 3'.

140 say, we found that most T. brucei telomere G-overhangs end in 5' TTAGGG 3', while a small portion of

141 By investigations of longer targets with overhangs exposed to the solution, we can demonstrate ap

142 protection involves the insertion of the 3' overhang facilitated by telomere repeat-binding factor 2

143 espect to stimulating Artemis activity at 3' overhangs, favoring the view that these NHEJ proteins ar

144 kness with this approach arises when melting overhanging features, which have no prior melted materia

145 Interestingly, MtDinG unwinds overhangs, flap structures, and forked duplexes but fail

146 3' overhang formation is thus a multistep, shelterin-contro

147 cleavage sites and each of the six expected overhangs formed at nascent termini adjacent to the clea

148 ein A (RPA), which binds single-stranded DNA overhangs formed by DSB resection.

149 into G-quadruplex arrangements inhibits the overhang from hybridizing with the RNA template of telom

150 NA binding region, DrII is able to remove 3' overhangs from RNA molecules closer to duplexes than do

151 However, the mechanism controlling G-overhang generation at human telomeres is poorly underst

152 d DNA2, which process DNA ends into 3' ssDNA overhangs; helicases such as BLM, which unwind DNA; and

153 ultispecies coprolite assemblage from a rock overhang in a montane river valley in southern New Zeala

154 DNA (dsDNA) junction and reels in the ssDNA overhang in an ATP-dependent manner.

155 the phosphate pocket while retaining the 3' overhang in the 3' pocket.

156 s, does not lead to further shortening of GT-overhangs in dna2 mutants.

157 Cdk1 induces persistent ssDNA-RPA overhangs in M phase, thereby preventing both classical

158 employs two restriction enzymes to generate overhangs in opposite orientations to which (single-stra

159 le-stranded DNA breaks to form long 3'-ssDNA overhangs in preparation for recombinational repair is c

160 telomere ends, generating transient long 3' overhangs in S/G2.

161 s blunt-ended or staggered-ended breaks with overhangs in the DNA.

162 emonstrated that the PCR enzyme fills the 5'-overhangs in the early cycles, and the product is then u

163 levels, implicating the involvement of 5'-C-overhangs in the HR-dependent pathway of telomere mainte

164 significantly affect the 5' TAGGGT 3'-ending overhangs, indicating that telomerase-mediated telomere

165 A 3' overhang is critical for the protection and maintenance

166 quence can be designed in a way that a small overhang is exposed to the electrode surface.

167 These data establish that the telomeric overhang is required for the protection of telomeres fro

168 more than one Srs2 molecule on the 3' ssDNA overhang is required to initiate DNA unwinding.

169 The universally conserved ssDNA overhang is sequence-specifically bound and regulated by

170 ffinity for binding to short single-stranded overhangs is much lower than for blunt DNA ends.

171 The dsDNA product, with a 3'-ssDNA overhang, is an optimal substrate for RecQ, which unwind

172 ivity is most efficient on DNA containing 3' overhangs, is facilitated by an insertion loop and conse

173 eighbor models of DNA, due to the additional overhang it engenders at the junction.

174 her preference for duplex substrates with 5' overhangs, it could also catalyze significant unwinding

175 Further, blunt termini, but not 3' overhangs, led to increased siRNAs from internal regions

176 d by the nucleotide state, ionic conditions, overhang length and overhang directionality.

177 The overhang length dictates the frequency (but not duration

178 G-overhang length increases with time after CTC1 disruptio

179 As the overhang length is reduced from 10 to 5 and 2 T for 24 a

180 S3DeltaC7 is independent of the substrate 3'-overhang length, implying that a monomeric form of the p

181 evels exhibit shorter telomeres, increased G overhang levels, and altered levels of non-homologous en

182 1, RAD52, and XRCC3 resulted in changes in C-overhang levels, implicating the involvement of 5'-C-ove

183 st that Drosophila telomeres possess a ssDNA overhang like the other eukaryotes, and that the termini

184 RT interaction with telomeres and leads to G-overhang loss.

185 The DNA contained a 15 nt overhang made entirely of thymidine residues adjacent to

186 In addition to 3' overhangs, many of these DNA ends unexpectedly form long

187 sults suggest that GQ formation of telomeric overhangs may contribute to suppression of DNA damage si

188 urfaces are created by designing an array of overhanging microdisks on a polymer film through two ste

189 f double-stranded (ds) DNA fragments with 3' overhangs mimicking double-strand breaks, and prevents r

190 rods with single-stranded oligo-thymine (T) overhangs modulating the end-to-end interactions.

191 The method is based on replication of overhanging nanostructures from an aluminum tube templat

192 demonstrate that the character of the two 3'-overhang nucleotides of the guide strand of siRNAs is a

193 gase IV, and PALF, PALF is able to resect 3' overhanging nucleotides and permit XRCC4-DNA ligase IV t

194 s of the hepatitis C virus (HCV) RNA with 3' overhanging nucleotides, masking the 5' terminal sequenc

195 -3', including a 3' terminal single-stranded overhang of 100-200 nucleotides that can fold into quadr

196 that preferentially acts on ssDNA and ssDNA-overhang of a partial duplex DNA.

197 We find that the overhang of a pre-miRNA is the key structural element th

198 e terminal 5'-triphosphate of RNA and the 3'-overhang of DNA results in a stable complex between p58C

199 higher when dsDNA is GC-rich or when the 3'-overhang of forked DNA is <15 bases.

200 In addition, we show that a single-stranded overhang of greater than 6 nucleotides is required for e

201 rt the prediction that in the full-length 3' overhang of human telomeres, G-quadruplexes with shortes

202 hat deoxyribonucleotides in the guide strand overhang of siRNAs have a negative impact on maintenance

203 igonucleotide homologous to the 3'-telomeric overhang of telomeres, elicits potent DNA-damage respons

204 tructure derived from the single-stranded 3'-overhang of the telomeric DNA is an attractive strategy

205 ferredoxin-like fold, which recognizes a 3'-overhang of U6 snRNA, and a preceding peptide, which bin

206 cifically targeting generic 2-nucleotide, 3' overhangs of any dsRNA.

207 tivity at DNA hairpins and at 5'- and 3'-DNA overhangs of duplex DNA, and this endonucleolytic activi

208 ease activity, cleaving the 3' single-strand overhangs of duplex RNA.

209 nuclease activity on single-stranded DNA or overhangs of duplex substrates.

210 3'-PG and 3'-phosphate termini on 1-base 3' overhangs of NCS-C-induced DSBs were readily detected in

211 either 5'- or 3'-single-stranded DNA (ssDNA) overhangs of undefined length.

212 moval of CTC1 results in elongation of the 3 overhang on the G-rich strand.

213 The effect of a target DNA overhang on the hybridization efficiency was shown to en

214 icase activity required a 3'-single-stranded overhang on the third strand and was dependent on ATP hy

215 ependent upon and guided by complementary 5' overhangs on the donor DNA.

216 Nucleotide overhangs on the electrode side affected the signal more

217 electrode side affected the signal more than overhangs on the solution side due to the greater insula

218 5'-overhang versus the same duplex with a 3'-overhang or without an overhang.

219 aired nicks, but, surprisingly, only when 5' overhangs or blunt ends can be generated.

220 ' single-stranded DNA overhang but not of 5' overhangs or blunt-ended DNA fragments.

221 in heterodimer, TauF4 is characterized by an overhanging peptide corresponding to the first of the fo

222 on of 3' DSBs, likely through recognition of overhang polarity by the Mre11 nuclease.

223 To better understand how overhang polarity of chromosomal DSBs affects NHEJ, we m

224 sults suggest distinct DSB handling based on overhang polarity that impacts NHEJ kinetics and fidelit

225 Like CTC1 and STN1, TEN1 is needed for G-overhang processing.

226 actor, binds POT1b and shortens the extended overhangs produced by Exo1, likely through fill-in synth

227 The 3' human telomeric overhang provides ample opportunities for the formation

228 After harvesting the complex, 3' overhang regions of the TRs were labeled with three dist

229 randed DNA and linear duplexes containing 5' overhangs relative to the 3' overhangs but not to blunt-

230 The 11 bp sDNA rods with 5- and 10-T overhangs remain in the I phase, consistent with their s

231 telomere damage signalling, nor in telomere overhang removal, which are critical for telomere fusion

232 break ends generates 3' single-stranded DNA overhangs required for homology-based DNA repair and act

233 n differ by up to three mismatches and three overhanging residues from their virtual center.

234 er specific to DNA polymerase containing the overhang sequence and the complementary blocker DNA, whi

235 ent target enzymes by simply redesigning the overhang sequence of detection probe, while keeping TaqM

236 can be estimated by the calculator from the overhang sequences or provided by the user from direct e

237 orked DNA, and the terminal 3'-OH of each 3' overhang serves as an attacking nucleophile during integ

238 nteractions between 11 bp sDNA rods with 2-T overhangs set in dramatically, and a novel 2D columnar N

239 ang elongation in mid-S phase, but it delays overhang shortening in late S/G2.

240 howed unwinding of only RNA duplexes with 5' overhangs showing 5'-to-3' polarity.

241 of DNA constructs with or without GQ in the overhang shows that GQ unfolding is achieved in 50-70% o

242 accompanied by a reduction in the telomeric overhang signal.

243 on by capturing two adjacent single-stranded overhangs simultaneously.

244 cated the Apollo nuclease in maintaining the overhang specifically at those telomeres generated by le

245 The G overhang spontaneously folds into various G-quadruplex (

246 strate that the presence and polarity of the overhang structure is a critical determinant of double-s

247 re synthesis is important for the telomere G-overhang structure.

248 ntially binds synthetic forked DNA or 3'-DNA overhang substrates resembling structures used during HR

249 ex bound to forked substrates than to single-overhang substrates.

250 Here, we determine the mechanism for 3' overhang synthesis in mouse cells by evaluating changes

251 in close proximity, such as the 3'-telomeric overhang, telomeric DNA bubbles and the D-loop at the ba

252 lly, dephosphorylation of the 5'-overhang/3'-overhang template reduced the efficiency of DNA repair w

253 , 3'-overhang/3'-overhang and 5'-overhang/5'-overhang templates were processed by resection of 3-5 ba

254 ermini promote processive cleavage, while 3' overhanging termini are cleaved distributively.

255 Our data suggest that blunt or 5'-overhanging termini engage Dicer's helicase domain to fa

256 ssential for cleaving dsRNA with blunt or 5'-overhanging termini, but not those with 3' overhangs, as

257 in is required for binding blunt, but not 3' overhanging, termini.

258 end in a short, single-stranded DNA (ssDNA) overhang that is recognized and bound by two telomere pr

259 alian telomeres contain a single-stranded 3' overhang that is thought to mediate telomere protection.

260 uently resected to form long single-stranded overhangs that can be repaired by mutagenic pathways.

261 Overhangs that contain ribonucleotides or 2'-O-methyl mo

262 d TbTR) and TbKu are required for telomere G-overhangs that end in 5' TTAGGG 3' but do not significan

263 by telomerase and resection to produce 3'-GT-overhangs that extend beyond the complementary 5'-CA-ric

264 blique) and the presence of Bruch's membrane overhanging the border tissue.

265 Ray Exposure ages demonstrate that the cliff overhanging the Chauvet cave has collapsed several times

266 ch to the full length of the human telomeric overhang, the presence of higher-order quadruplex-quadru

267 r suppressed stacking interactions with 10-T overhangs, the volume fraction of sDNA at which the 2D i

268 Although FDM1 and IDN2 bind RNAs with 5' overhangs, their functions in the RdDM pathway remain to

269 sembly of short DNA blocks with dinucleotide overhangs through a simple ligation process.

270 estores the canonical end structure (2-nt 3' overhang) through mono-uridylation, thereby promoting mi

271 use cells by evaluating changes in telomeric overhangs throughout the cell cycle and at leading- and

272 amer and thereby bring the end of the G-rich overhang to close proximity to Ag NCs, resulting in a si

273 These overhangs, together with the RADiation sensitive51 (RAD5

274 f roof and gutter hygiene and elimination of overhanging tree branches and other structures where pos

275 or group II pre-miRNAs, which have a 1-nt 3' overhang, TUT7 restores the canonical end structure (2-n

276 of a polymerase bound to a DNA end with a 3' overhang, two metal ions, and an incoming nucleotide but

277 ding an RNA duplex with a single-stranded 5'-overhang versus the same duplex with a 3'-overhang or wi

278 A longer target DNA overhang was found to provide a better response.

279 When the overhang was on the electrode side the signal enhancemen

280 signal enhancement was greater than when the overhang was on the solution side due to the increased t

281 Bruch's membrane overhang was regionally present in the majority of patie

282 vidual duplex DNA molecules with symmetrical overhangs was carried out by pulling one strand of the d

283 A (pdDNA) constructs with different 5' ssDNA overhangs, we show that BLM localizes in the vicinity of

284 rhang, blunt/5'-overhang, and 3'-overhang/5'-overhang were predominantly repaired with fill-in and li

285 The results showed that compatible 5'-overhangs were accurately joined in all mutants, that KU

286 C-rich overhangs were far more prevalent in tumor cells engaged

287 C-overhangs were prominent in G1/S arrested as well as ter

288 ays only edited DNA when 5' sgRNA nucleotide overhangs were removed, suggesting a novel processing me

289 test if the abasic site is within a short 5' overhang, when this activity is necessary and sufficient

290 ssed DNA end with a 40-nucleotide (nt) ssDNA overhang, where it localized to the ssDNA-dsDNA junction

291 omeres terminate with a single-stranded 3' G overhang, which can be recognized as a DNA damage site b

292 e and facilitate the formation of a 3' ssDNA overhang, which is a necessary intermediate for recombin

293 nded DNA or duplex DNA with a four-base pair overhang, which is consistent with the known structure o

294 ent directions producing single-stranded DNA overhangs, which are potential intermediates for the syn

295 ed to yield long single-stranded DNA (ssDNA) overhangs, which are quickly bound by replication protei

296 led duplexes containing a 5' single-stranded overhang with an excess of unlabeled DNA to initiate the

297 etitive DNA sequence terminating in an ssDNA overhang with many associated proteins.

298 rtebrates, followed by a single-stranded DNA overhang with the same sequence.

299 stimulated Artemis to cut near the end of 3' overhangs without the involvement of other NHEJ proteins

300 e advantages enable the fabrication of large overhangs without the use of supports, reduction of the