ライフサイエンスコーパス: oxacillin

1 The two VMEs were with oxacillin.

2 ree very major errors (VMEs; 1.7%) were with oxacillin.

3 ed with results obtained by CLSI methods for oxacillin.

4 sion septum was prevented after acylation by oxacillin.

5 d by their size and their susceptibility for oxacillin.

6 articularly sensitive to rifampin but not to oxacillin.

7 determine the susceptibility of S. aureus to oxacillin.

8 tional methods are functionally resistant to oxacillin.

9 nitive therapy with cefazolin, nafcillin, or oxacillin.

10 olates) were evaluated for susceptibility to oxacillin.

11 our of six tested isolates were resistant to oxacillin.

12 acillin, 25mug/kg dicloxacillin and 30mug/kg oxacillin.

13 s less expensive for outpatient therapy than oxacillin.

14 llin and 946/993 (95.3%) were susceptible to oxacillin.

15 ) received ceftriaxone and 50 (40%) received oxacillin.

16 r infections treated with ceftriaxone versus oxacillin.

17 cell wall stability, such as ampicillin and oxacillin.

18 determined by using Mueller-Hinton agar with oxacillin.

19 llin (6 microg), methicillin (5 microg), and oxacillin (1 microg) disks.

20 or errors (one each with chloramphenicol and oxacillin), 1 major error (chloramphenicol), and 15 mino

21 3.6%), coagulase-negative staphylococci with oxacillin (2 of 74, 2.7%), gram-negative bacilli with ce

22 ntration (sub-MIC) levels, lowers the MIC of oxacillin (2) against a number of MRSA strains by up to

23 le/trimethoprim, 77.5%; levofloxacin, 58.5%; oxacillin, 54.7%; ciprofloxacin, 51.0%; gatifloxacin, 51

24 ing intermediate) for hVISA were as follows: oxacillin, 82%; erythromycin, 82%; clindamycin, 73%; lev

25 ompared with patients receiving nafcillin or oxacillin, after controlling for other factors.

26 ared with patients who received nafcillin or oxacillin, after controlling for other factors.

27 ion with 4% salt (the conditions used in the oxacillin agar screen method), the oxacillin MICs of 16

28 Due to the poor sensitivity of the oxacillin agar screen plate for predicting resistance in

29 the 51 strains, 44 (86%) did not grow on the oxacillin agar screen plate, broth microdilution MICs we

30 eference broth microdilution method, and the oxacillin agar screen plate.

31 Another seven strains did grow on the oxacillin agar screen plate.

32 culated onto a pair of commercially prepared oxacillin agar screen plates containing 6 microg of oxac

33 Louis, Mo.), and the oxacillin agar screen test for detection of MRSA, with P

34 The oxacillin agar screen test, the VITEK-1 system, the VITE

35 n, 100/100; Velogene, 100/100; Vitek, 95/97; oxacillin agar screen, 90/92; disk diffusion, 100/89; Mi

36 ethods (broth microdilution, disk diffusion, oxacillin agar screen, MicroScan conventional panels, Mi

37 ant and should be verified as susceptible by oxacillin agar screening with incubation for 48 h.

38 itive therapy with cefazolin vs nafcillin or oxacillin among patients with MSSA infections complicate

39 Oxacillin and cefoxitin disk and MIC tests were evaluate

40 in screen agar (BD Diagnostics, Sparks, MD), oxacillin and cefoxitin Etests (AB Biodisk, Solna, Swede

41 The results for oxacillin and cefoxitin generated by the automated syste

42 found that the three tested systems, Vitek 2 oxacillin and cefoxitin testing and cefoxitin disk susce

43 The Vitek 2 oxacillin and cefoxitin tests had very major error rates

44 ted against oxacillin and the combination of oxacillin and clavulanic acid with the Vitek GPS-SA card

45 An additional PBP inducible by oxacillin and corresponding to S. aureus PBP2A was ident

46 is isolate was phenotypically susceptible to oxacillin and did not contain the mecA gene by Southern

47 the discovery of synergistic action between oxacillin and manuka honey against methicillin-resistant

48 However, oxacillin and methicillin disk testing remain excellent

49 ompared to an antistaphylococcal penicillin (oxacillin and nafcillin) or first-generation cephalospor

50 y, the efficacy of antibiotics (doxycycline, oxacillin and rifampicin) in preventing Staphylococcus a

51 ere 1 to 8 micrograms/ml were tested against oxacillin and the combination of oxacillin and clavulani

52 rA double mutants in the endocarditis model, oxacillin and vancomycin treatment of the mgrA/sarA doub

53 owed high and homogeneous resistance to both oxacillin and vancomycin.

54 ganism is typically resistant to penicillin, oxacillin, and erythromycin (the latter mediated by msrA

55 robials, including cefoxitin, ciprofloxacin, oxacillin, and methicillin.

56 nvolves the use of three disks (methicillin, oxacillin, and penicillin) or two disks (methicillin and

57 egrees C, and a higher relative affinity for oxacillin as compared with cefoxitin.

58 Clinical Laboratory Standards methods using oxacillin as the class drug.

59 Oxacillin bactericidal assays showed that mecA- and PBP

60 cci present in the specimen are resistant to oxacillin (based on amplification of the mecA gene).

61 ve CoNS strains but gave better results than oxacillin BMD or oxacillin DD for mecA-negative strains

62 rolyzed benzylpenicillin-, methicillin-, and oxacillin-bound NDM-1 have been solved to 1.8, 1.2, and

63 ee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoints for broth microdilution and disk d

64 cefoxitin DD test performed equivalently to oxacillin broth microdilution (BMD) and to oxacillin DD

65 ally, a cefoxitin disk diffusion test and an oxacillin broth microdilution assay were examined.

66 The cefoxitin disk diffusion and oxacillin broth microdilution assays categorized 100% an

67 For four of the latter group of strains, oxacillin broth microdilution MICs were > 4 micrograms/m

68 flow cytometry (FCM) following incubation in oxacillin broth.

69 ne isolate was determined to be resistant to oxacillin by reference broth microdilution testing (MIC,

70 reus (MRSA) infections by demonstrating that oxacillin can be used to significantly attenuate the vir

71 First-line MSSA therapies (nafcillin, oxacillin, cefazolin) are generally avoided in the 10% o

72 he crystal structures of three beta-lactams (oxacillin, cefepime, ceftazidime) complexes with PBP2a-e

73 ty against beta-lactam antibiotics including oxacillin, cloxacillin, and dicloxacillin, but not abaca

74 iofur), ampicillin, cefazolin, penicillin G, oxacillin, cloxacillin, naficillin, and dicloxacillin.

75 arge detached clumps were highly tolerant to oxacillin compared with exponential-phase planktonic cul

76 ase and exhibit borderline susceptibility to oxacillin, comprised a greater percentage of the 120 wou

77 ntiated by FCM after 2 h of incubation in an oxacillin-containing liquid culture medium.

78 S. aureus isolates were incubated in oxacillin-containing Mueller-Hinton broth, stained using

79 ut gave better results than oxacillin BMD or oxacillin DD for mecA-negative strains of CoNS.

80 The cefoxitin DD test is preferred over the oxacillin DD test for predicting mecA-mediated oxacillin

81 o oxacillin broth microdilution (BMD) and to oxacillin DD tests among S. aureus and mecA-positive CoN

82 This effect is based primarily on the oxacillin-dependent repression of the accessory gene reg

83 reus strains to beta-lactam antibiotics (eg, oxacillin) depends on the production of penicillin-bindi

84 have shown a reasonable correlation between oxacillin disc and automated sensitivity testing, changi

85 e use of MSA containing lipovitellin with an oxacillin disk (MSAL(Ox)).

86 methods were compared with those obtained by oxacillin disk diffusion.

87 with those obtained by a PCR-based assay and oxacillin disk diffusion.

88 solates and that laboratories should perform oxacillin disk or MIC tests of these isolates when they

89 MRSA isolates were identified by the oxacillin disk-diffusion method.

90 d be used in conjunction with a 1-micrograms oxacillin disk.

91 e diameters for methicillin, penicillin, and oxacillin disks; (ii) the sum of the zone diameters for

92 methicillin-resistant S. aureus, addition of oxacillin does not result in delocalization of PBP2 indi

93 urrent infections compared with nafcillin or oxacillin for MSSA infections complicated by bacteremia.

94 In this comparison of ceftriaxone versus oxacillin for MSSA osteoarticular infections, there was

95 ylpenicillin, cloxacillin, dicloxacillin and oxacillin) from cows' milk, without prior protein precip

96 fepime, piperacillin-tazobactam, ampicillin, oxacillin, gentamicin, and a combination of gentamicin/p

97 t also potentiated the anti-MRSA activity of oxacillin in a synergistic fashion, resulting in an 8-fo

98 Live pneumococci, oxacillin-killed pneumococci, and pneumococcal cell wall

99 64.7 murine macrophages with pneumococci and oxacillin led to significantly higher inducible nitric o

100 bh variants display increased sensitivity to oxacillin (methicillin) as well as susceptibility to com

101 reus isolates were recovered; 208 (59%) were oxacillin (methicillin) susceptible and 146 (41%) were o

102 (Ox)), use of Mueller-Hinton agar containing oxacillin (MHA(Ox)), and the use of MSA containing lipov

103 point, the CLSI VET01-S2 S. pseudintermedius oxacillin MIC and disk breakpoints, and the European Com

104 No correlation was found between oxacillin MIC and vancomycin or daptomycin MIC.

105 S25 coagulase-negative Staphylococcus (CoNS) oxacillin MIC breakpoint and cefoxitin disk breakpoint,

106 presence of resistance mediated by mecA, the oxacillin MIC breakpoint for defining resistance in CoNS

107 hylococcus aureus/Staphylococcus lugdunensis oxacillin MIC breakpoints and cefoxitin disk and MIC bre

108 specificity, respectively, were as follows: oxacillin MIC by broth microdilution, 94.4% and 96.7%; o

109 Phoenix system, 7 on the Vitek 2 system), an oxacillin MIC in the susceptible range was correctly cha

110 Thus, an oxacillin MIC of > or = 2 micrograms/ml indicated resist

111 eporting isolates that test resistant by the oxacillin MIC or cefoxitin disk test as oxacillin resist

112 AT demonstrated 99% agreement with MicroScan oxacillin MIC results for 388 isolates of S. aureus.

113 mmercial automated susceptibility test panel oxacillin MIC results were also evaluated and demonstrat

114 For a different four isolates, the oxacillin MIC was < or =0.25 microg/ml on the Vitek GPS

115 /ml), one isolate was inducibly resistant to oxacillin (MIC of 16 microg/ml after overnight induction

116 lococcus aureus that developed resistance to oxacillin (MIC up to 16 mug/ml).

117 ed in the oxacillin agar screen method), the oxacillin MICs of 16 of the mecA-negative strains increa

118 ns of expression class 1 or 2 (demonstrating oxacillin MICs of 4 to >16 microg/ml) and 36 mecA-negati

119 The oxacillin MICs of the latter strains were 0.25 to 4 micr

120 l 61 challenge strains of CoNS for which the oxacillin MICs were 0.5 to 2 microg/ml were tested in a

121 ococcus aureus strains lacking mec for which oxacillin MICs were 1 to 8 micrograms/ml were tested aga

122 and 41 non-mecA-producing strains for which oxacillin MICs were near the susceptible breakpoint.

123 0 mug/ml) and a somewhat lower resistance to oxacillin (minimal inhibitory concentration = 200 mug/ml

124 use, each containing 4% NaCl and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied w

125 en plates prepared in house with 6 microg of oxacillin/ml and 4% NaCl using the four different inocul

126 ented with 4% NaCl and containing 0.6 microg oxacillin/ml and incubation at 35 degrees C for 48 h (on

127 nitol-salt agar (MSA), use of MSA containing oxacillin (MSA(Ox)), use of Mueller-Hinton agar containi

128 bactam and tazobactam, the reactions between oxacillin or 6alpha-hydroxyisopropylpenicillinate (both

129 nts can be predicted from the penicillin and oxacillin or cefoxitin results.

130 Routine testing of penicillin and oxacillin or cefoxitin should be used to infer susceptib

131 lococcal beta-lactams except for penicillin, oxacillin or cefoxitin, and ceftaroline.

132 comycin and a beta-lactam (either nafcillin, oxacillin, or cefazolin) for staphylococcal bacteremia m

133 FP), cloxacillin (CLO), dicloxacillin (DCL), oxacillin (OXA) and phenoxymethylpenicillin (PEV), in Ma

134 (AMP), penicillin G (PG), penicillin V (PV), oxacillin (OXA), cloxacillin (CLO), dicloxacillin (DICLO

135 arried the mecA gene but were susceptible to oxacillin (oxacillin-susceptible methicillin-resistant S

136 0.04), erythromycin (P<0.0001), methicillin/oxacillin (P<0.0001), ampicillin (P = 0.01), and ceftria

137 31 of 74 [42%] ceftriaxone vs 25 of 50 [50%] oxacillin; P = .4).

138 43 of 56 [77%] ceftriaxone vs 26 of 32 [81%] oxacillin; P = .6).

139 50 of 60 [83%] ceftriaxone vs 32 of 37 [86%] oxacillin; P = .7) and >6 months (43 of 56 [77%] ceftria

140 elated beta-lactam antibiotics (amoxicillin, oxacillin, penicillin G).

141 in agar screen plates containing 6 microg of oxacillin per ml and 4% NaCl.

142 decreased 10-fold (from 6.0 to 0.6 microg of oxacillin per ml) for the agar swab screen method, fully

143 e media containing either 0.625 microgram of oxacillin per ml, 40 microgram of cephalexin per ml, or

144 reen containing 4% NaCl plus-6 micrograms of oxacillin per ml, the sensitivities in detecting the 44

145 esistance and biofilm formation in vitro and oxacillin persistence in an experimental endocarditis mo

146 fashion, resulting in an 8-fold increase in oxacillin potency, for a MIC of 16 mug mL(-1).

147 nce methods: mecA gene detection and MICs of oxacillin previously determined by broth microdilution a

148 ontrols) displayed significant reductions in oxacillin resistance and biofilm formation in vitro and

149 LAT provided rapid and reliable detection of oxacillin resistance and proved a useful adjunct to the

150 phenotypic and genotypic characteristics of oxacillin resistance both in vitro and in an experimenta

151 ncreasing vancomycin MICs and the changes in oxacillin resistance could be reproduced by appropriate

152 Oxacillin resistance depended on the overtranscribed S.

153 tection was reported but was correlated with oxacillin resistance in a species other than S. aureus o

154 tination test for detection of mecA-mediated oxacillin resistance in canine staphylococci.

155 were evaluated for their abilities to detect oxacillin resistance in coagulase-negative staphylococci

156 software version VTK-R07.01 for detection of oxacillin resistance in coagulase-negative staphylococci

157 d a sensitivity and specificity at detecting oxacillin resistance in CoNS at a level that was accepta

158 These results suggested that sarA regulates oxacillin resistance in mecA-positive MRSA.

159 s spp., had low sensitivity for detection of oxacillin resistance in members of the Staphylococcus in

160 acillin DD test for predicting mecA-mediated oxacillin resistance in S. aureus and CoNS.

161 foxitin DD test for predicting mecA-mediated oxacillin resistance in staphylococci and revised Table

162 usion (DD) test for predicting mecA-mediated oxacillin resistance in staphylococci was assessed durin

163 transcription of mecA, the gene required for oxacillin resistance in staphylococci, was quantified in

164 hring, West Sacramento, CA) for detection of oxacillin resistance in Staphylococcus aureus.

165 and mecA real-time PCR for the detection of oxacillin resistance in Staphylococcus aureus.

166 ) were evaluated for their ability to detect oxacillin resistance in Staphylococcus aureus.

167 ve and specific for detecting staphylococcal oxacillin resistance in the clinical microbiology labora

168 Major changes were observed in the oxacillin resistance phenotype of several of the isolate

169 ar dilution methods, more CoNS isolates with oxacillin resistance related to the mecA gene were detec

170 ens were also cultured on CHROMagar MRSA and oxacillin resistance screening agar base (ORSAB) and in

171 Oxacillin resistance was induced in 6 of the 10 MecA+ is

172 attributed to the Vitek 2 system overcalling oxacillin resistance.

173 tance as elsewhere in the country, including oxacillin resistance; however, the rate of fluoroquinolo

174 aboratories were able to detect methicillin (oxacillin) resistance in Staphylococcus aureus, high-lev

175 (methicillin) susceptible and 146 (41%) were oxacillin resistant (MRSA).

176 ulase-negative staphylococci tested, 81 were oxacillin resistant and 37 oxacillin susceptible by the

177 the oxacillin MIC or cefoxitin disk test as oxacillin resistant, following such guidelines produces

178 S were falsely classified as methicillin and oxacillin resistant.

179 cillin sensitive and 1 isolate was MecA- but oxacillin resistant.

180 rocedural infections from which methicillin (oxacillin)-resistant Staphylococcus aureus (MRSA) strain

181 ureus PBP2A, and was greatly overproduced in oxacillin-resistant clinical isolate S. sciuri SS37 and

182 to S. aureus PBP2A was identified in another oxacillin-resistant clinical isolate, S. sciuri K3, whic

183 3 oxacillin-sensitive S. aureus isolates, 17 oxacillin-resistant CNS, and 7 oxacillin-sensitive CNS).

184 All 249 oxacillin-resistant isolates gave strong positive reacti

185 vate dehydrogenase gene were detected in the oxacillin-resistant isolates.

186 tant S. aureus (MRSA) and 27 were borderline oxacillin-resistant S. aureus (BORSA).

187 Both methods provided reliable detection of oxacillin-resistant S. aureus and facilitated the discov

188 from each of 60 staphylococcal isolates (13 oxacillin-resistant S. aureus isolates, 23 oxacillin-sen

189 for 48 h) of the methods tested revealed all oxacillin-resistant S. aureus isolates, and no growth fa

190 mmended for the detection of vancomycin- and oxacillin-resistant S. aureus.

191 Locally generated data showed that oxacillin-resistant staphylococci (57.0% overall) had sl

192 lin-susceptible staphylococci, and 97.4% for oxacillin-resistant staphylococci.

193 n-susceptible Staphylococcus aureus (14.3%), oxacillin-resistant Staphylococcus aureus (4.4%), coagul

194 Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodst

195 Oxacillin-resistant Staphylococcus aureus (ORSA) is a vi

196 In the present study, 21 oxacillin-resistant Staphylococcus aureus (ORSA) isolate

197 Oxacillin-resistant Staphylococcus aureus (ORSA) was fou

198 Pure cultures were noted for 20% of oxacillin-resistant Staphylococcus aureus cultures, 9.2%

199 methods for the detection of vancomycin- and oxacillin-resistant Staphylococcus aureus in </=6 h: (i)

200 with lower susceptibility rates seen for the oxacillin-resistant strains.

201 s of antibiotics; 46.6% of CNS isolates were oxacillin-resistant, and they were more resistant to ant

202 Staphylococcus aureus breakpoints to report oxacillin results for Staphylococcus saprophyticus.

203 The oxacillin results interpreted by the VET01-S2 (disk and

204 The oxacillin-salt agar screen (OS) test, the reference brot

205 cillin resistance was confirmed by growth on oxacillin-salt screening agar.

206 s with discrepant results was done using BBL oxacillin screen agar (BD Diagnostics, Sparks, MD), oxac

207 g a blood culture pellet was compared to the oxacillin screen agar method using isolated colonies.

208 MIC by broth microdilution, 94.4% and 96.7%; oxacillin screen agar, 94.3% and 96.7%; PBP2' latex aggl

209 Those lots of oxacillin screen medium that fail to grow heteroresistan

210 ly the inoculation methods to be used in the oxacillin screen test for Staphylococcus aureus, we test

211 biology system (BD Diagnostics, Sparks, MD), oxacillin screening agar (BD Diagnostics), BBL CHROMagar

212 wo isolates that were mecA positive but were oxacillin sensitive according to conventional methods.

213 0 coagulase-negative isolates were MecA+ but oxacillin sensitive and 1 isolate was MecA- but oxacilli

214 e 10 MecA+ isolates previously classified as oxacillin sensitive.

215 isolates, 17 oxacillin-resistant CNS, and 7 oxacillin-sensitive CNS).

216 ere more resistant to antibiotics than their oxacillin-sensitive counterparts (P < .001), including f

217 3 oxacillin-resistant S. aureus isolates, 23 oxacillin-sensitive S. aureus isolates, 17 oxacillin-res

218 hylococcus aureus isolates and found that an oxacillin-sensitive/cefoxitin-resistant profile had a se

219 2 for expression of high level resistance to oxacillin, suggesting that the PBP2A homolog may prefere

220 haracterized strains of CoNS were tested for oxacillin susceptibility by the NCCLS broth microdilutio

221 rrent disk diffusion breakpoint criteria for oxacillin susceptibility for S. aureus showed a very-maj

222 Positive correlation between methicillin and oxacillin susceptibility test results and the detection

223 After screening for oxacillin susceptibility, MRSA and selected methicillin-

224 ved by AST devices was "cefoxitin resistance/oxacillin susceptibility," ranging from 54.1% (Phoenix)

225 rror rate when cefoxitin was used to predict oxacillin susceptibility.

226 change in the function of PBP2a resulting in oxacillin susceptibility.

227 i tested, 81 were oxacillin resistant and 37 oxacillin susceptible by the Vitek II assay compared wit

228 The GPS-SA card classified 86% of strains as oxacillin susceptible.

229 tested by agar dilution, 24 of 28 (86%) were oxacillin susceptible.

230 ycin resistance rates of 26.0% and 55.0% for oxacillin-susceptible and -resistant Staphylococcus aure

231 Three of the 139 oxacillin-susceptible isolates were also strongly positi

232 Ninety-two oxacillin-susceptible isolates were selected to assess t

233 The three oxacillin-susceptible isolates with strongly positive re

234 mecA gene but were susceptible to oxacillin (oxacillin-susceptible methicillin-resistant S. aureus [O

235 SA800, seven of which included both ORSA and oxacillin-susceptible S. aureus isolates.

236 amicin, and tetracycline were active against oxacillin-susceptible staphylococci (82 to 99% susceptib

237 method was 91.5% for enterococci, 99.8% for oxacillin-susceptible staphylococci, and 97.4% for oxaci

238 The predominant aerobic organisms were oxacillin-susceptible Staphylococcus aureus (14.3%), oxa

239 avancin MIC(90) values (0.19 microg/ml) than oxacillin-susceptible strains (0.125 microg/ml).

240 Oxacillin-susceptible, mecA-positive Staphylococcus aure

241 tes in AST systems based on cefoxitin and/or oxacillin testing yielded overall positive agreements wi

242 d between the response of the hemB mutant to oxacillin therapy and that of the parent strain in any t

243 In contrast, oxacillin therapy did not significantly reduce bacterial

244 invasive pneumonia and sepsis, we show that oxacillin-treated MRSA strains are significantly attenua

245 discontinued due to toxicity (9 of 50 [18%] oxacillin vs 3 of 74 [4%] ceftriaxone; P = .01).

246 and 6 microg of oxacillin/ml (0.6-microg/ml oxacillin was also studied with MH agar prepared in-hous

247 Oxacillin was ineffective for MSSA colonization in appro

248 Oxacillin was more often discontinued due to toxicity (9

249 itivity and specificity compared to those of oxacillin were 97.3% and 100%, respectively.

250 ubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling

251 Patients receiving oxacillin were more likely to have it discontinued due t

252 Inoculum volume and induction with oxacillin were PBP 2a testing variables.

253 ratories generate disk diffusion results for oxacillin when testing S. aureus ATCC 25923 and S. pneum

254 was inhibited by the beta-lactam antibiotic oxacillin, which slowed inactivation of daptomycin and e