ライフサイエンスコーパス: oxaloacetate

1 fixation and increased ATP yield (1 ATP per oxaloacetate).

2 ion of malate supplied from the cytoplasm to oxaloacetate.

3 e capacity to accommodate the TCA metabolite oxaloacetate.

4 but only AOX1A is additionally activated by oxaloacetate.

5 ysed reversible interconversion of malate to oxaloacetate.

6 l-CoA to pyruvate to yield propionyl-CoA and oxaloacetate.

7 -CoA to pyruvate, yielding propionyl-CoA and oxaloacetate.

8 ear high enough to abolish any channeling of oxaloacetate.

9 ehydrogenase specifically oxidizes malate to oxaloacetate.

10 izing 2-methylcitrate from propionyl-CoA and oxaloacetate.

11 ghly specific for the oxidation of malate to oxaloacetate.

12 itself or its temperature-labile substrate, oxaloacetate.

13 drogenase does not exhibit any channeling of oxaloacetate.

14 citrate, succinate, alpha-ketoglutarate, and oxaloacetate.

15 etyl-coenzyme A, in its ternary complex with oxaloacetate.

16 ent the negatively charged carboxyl group in oxaloacetate.

17 ibution from either plasma glucose or muscle oxaloacetate.

18 activity and was blocked by the CS substrate oxaloacetate.

19 m and an active site variant in complex with oxaloacetate.

20 action mechanism promotes decarboxylation of oxaloacetate.

21 miting step in the biosynthesis of AcCoA and oxaloacetate.

22 tamate abrogated inhibition of Complex II by oxaloacetate.

23 K) kinetic isotope effect observed on C-4 of oxaloacetate [(13)(V/K) = 1.0117 +/- 0.0005] indicates t

24 ic enzyme (275%); elevated concentrations of oxaloacetate (150%), malate (250%), citrate (140%), and

25 ial extracts was made to oscillate by adding oxaloacetate (5 micromol/l) to inhibit the enzyme.

26 ne with (k(cat)/K(m)(pyruvate))/(k(cat)/K(m)(oxaloacetate)) = 6.1 x 10(-9) to one with that ratio = 2

27 cosubstrates, 2-oxoglutarate, pyruvate, and oxaloacetate, Ab-ArAT4 possesses strong substrate prefer

28 mino acid sequence identity to the confirmed oxaloacetate acetyl hydrolase (OAH), an enzyme that belo

29 ough several different biochemical pathways, oxaloacetate acetylhydrolase (OAH)-catalyzed hydrolytic

30 Finally, deletion of the oxaloacetate acetylhydrolase gene in H915-1 eliminated o

31 exhibited a 52 +/- 7% reduction in cytosolic oxaloacetate, an 83 +/- 4% reduction in malonyl-CoA leve

32 MgATP-dependent carboxylation of pyruvate to oxaloacetate, an important anaplerotic reaction in mamma

33 tate with pyruvate, alpha-ketoglutarate, and oxaloacetate and (ii) mercaptopicolinate and pyruvate.

34 ed should cleave protein bound citryl-CoA to oxaloacetate and a protein-bound CoA derivative.

35 bisubstrate adduct indicate that each of the oxaloacetate and acetyl-CoA substrates is bound to an in

36 routes glutamine metabolism to generate both oxaloacetate and acetyl-CoA, enabling persistent tricarb

37 and MgATP-dependent cleavage of citrate into oxaloacetate and acetyl-CoA, representing a key step in

38 Simple dehydration of tartrates to oxaloacetate and an ensuing decarboxylation to form pyru

39 nd the TCA cycle, such as pyruvate, acetate, oxaloacetate and cholesterol.

40 enolpyruvate then reacts with CO(2) to form oxaloacetate and complete the reaction.

41 ates determined at varying concentrations of oxaloacetate and fixed concentrations of oxamate reveale

42 al transport fluxes of pyruvate, acetyl-CoA, oxaloacetate and glycine.

43 into acetyl-CoA and toward the formation of oxaloacetate and into the gluconeogenic pathway.

44 es the cell with only one pathway, involving oxaloacetate and l-glutamate, for de novo synthesis of h

45 ve determined the ternary complex bound with oxaloacetate and magnesium, revealing some of the conser

46 ed production of the TCA cycle intermediates oxaloacetate and NADPH, and impaired oxygen consumption.

47 complex ferricyanide, and 3) the keto-acids oxaloacetate and pyruvate (and phosphoenolpyruvate, a me

48 malate or that cannot convert malate to both oxaloacetate and pyruvate are also avirulent and protect

49 s and identified a high futile cycle between oxaloacetate and pyruvate, indicating a highly active in

50 virulence, malate must be converted to both oxaloacetate and pyruvate.

51 nitor the PC-catalyzed formation of [4-(13)C]oxaloacetate and subsequent transfer of (13)CO(2) from o

52 oor or moderate KDM5B inhibitors, except for oxaloacetate and succinate, which were shown to compete

53 0 forms a hydrogen bond with the carbonyl of oxaloacetate and the alcohols of the citryl-coenzyme A a

54 led pyruvate leads to formation of [1,2-13C2]oxaloacetate and to multiply labeled glutamate and succi

55 Structural analyses of the complexes with oxaloacetate and with a bisubstrate adduct indicate that

56 d vesicles catalyzed the exchange of malate, oxaloacetate, and aspartate for phosphate plus a proton

57 y the TCA intermediates alpha-ketoglutarate, oxaloacetate, and pyruvate, confirming that in infected

58 ate to pyruvate, via pyruvate carboxylase to oxaloacetate, and via PCK2 to phosphoenolpyruvate.

59 olase (OAH)-catalyzed hydrolytic cleavage of oxaloacetate appears to be an especially important route

60 In the aerobic regime, 75% of mitochondrial oxaloacetate arises from anaplerotic carboxylation of py

61 oxidative decarboxylation was stepwise, with oxaloacetate as an intermediate, or concerted.

62 is essential for the conversion of malate to oxaloacetate as part of the proper functioning of the Kr

63 that only d-tartrate is dehydrated, yielding oxaloacetate as product.

64 d [U-(13)C]aspartate is formed from [U-(13)C]oxaloacetate, as is [U-(13)C]lactate.

65 iet, HFD-fed mice displayed higher levels of oxaloacetate, aspartate, and malate, along with increase

66 ubsite, and uncouples the decarboxylation of oxaloacetate at subsite 2 from the formation of ATP at s

67 dehydrogenase (MDH) to assess the chances of oxaloacetate being channeled between the MDH and CS acti

68 d with the specific example of channeling of oxaloacetate between Escherichia coli aspartate aminotra

69 mitochondrial malate dehydrogenase channels oxaloacetate between the active sites.

70 Pyruvate and oxaloacetate bind to the 2-oxoglutarate site of HIF-1alp

71 through carbonyl bond polarization, not just oxaloacetate binding in the active site, is required for

72 enzyme A but also forms the back wall of the oxaloacetate-binding site.

73 the cytoplasm where it can be converted into oxaloacetate by aspartate transaminase (GOT1).

74 the enzyme fumarase and further oxidized to oxaloacetate by malate dehydrogenase with the accompanyi

75 Furthermore, the exclusive formation of oxaloacetate by phosphoenolpyruvate (PEP) carboxylation

76 f these mutants can activate the carbonyl of oxaloacetate by polarization.

77 is of 2-methylcitrate from propionyl-CoA and oxaloacetate by the PrpC protein.

78 The D375 mutants polarize the oxaloacetate carbonyl as well as wild-type.

79 olpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylating), EC 4.1.1.3

80 olpyruvate carboxylase [PEPC; orthophosphate:oxaloacetate carboxy-lyase (phosphorylating), EC 4.1.1.3

81 lely dependent on the hydrolytic cleavage of oxaloacetate catalyzed by OAH.

82 athway for the interconversion of malate and oxaloacetate catalyzed by the enzyme malate dehydrogenas

83 ated by a carbamylated lysine, except in the oxaloacetate complex in which the product's carboxylate

84 The dicarboxylate ligand in oxaloacetate-containing crystals appears to be the same

85 on the enzymatic conversion of glyoxylate to oxaloacetate coupled to the reduction of oxidized nicoti

86 Stimulation of this oxaloacetate decarboxylase activity in the presence of s

87 ervation with carboxyltransferase domains in oxaloacetate decarboxylase and transcarboxylase, the str

88 ceptor domain from the Klebsiella pneumoniae oxaloacetate decarboxylase are extracted with 5 M urea o

89 Aspartate aminotransferase and oxaloacetate decarboxylase were less effective competito

90 ge amounts of aspartate aminotransferase and oxaloacetate decarboxylase, as competing enzymes for the

91 inotransferase and then decarboxylation with oxaloacetate decarboxylase.

92 pyruvate, indicating a highly active in vivo oxaloacetate decarboxylase.

93 bject to enzymatic decarboxylation; however, oxaloacetate decarboxylases (ODx) were so far not identi

94 sults presented here indicate that dedicated oxaloacetate decarboxylases exist in eukaryotes.

95 n of the two enzymes required are described; oxaloacetate decarboxylating malic dehydrogenase is also

96 atase to convert fumarate to malate and uses oxaloacetate decarboxylating malic dehydrogenase to conv

97 no effect on the rate of biotin-independent oxaloacetate decarboxylation.

98 indicative of a glyoxylate-induced state of oxaloacetate deficiency, acetate overload, and ketoacido

99 ansfer preceding hydride transfer (malate to oxaloacetate direction), (2) the existence of two transi

100 xpressed to access the one-step synthesis of oxaloacetate directly from phosphoenolpyruvate without p

101 (alpha)-H cleavage, ketimine hydrolysis, and oxaloacetate dissociation to the rate limitation with th

102 cetyl cysteine or the TCA cycle intermediate oxaloacetate efficiently rescues Gln starvation-induced

103 tterns of Asp and Thr suggested formation of oxaloacetate exclusively via the phosphoenolpyruvate car

104 as a complete, albeit bifurcated, TCA cycle; oxaloacetate flows to succinate both through citrate/alp

105 erate reductive power (NADPH) and to restore oxaloacetate for continued TCA cycle function (anapleros

106 ter, respectively, whereas those for GTP and oxaloacetate (for the phosphoenolpyruvate formation acti

107 bound PEP, brought subtle effects, lowering oxaloacetate formation rate but enhancing PEP formation

108 eaction which produces acetyl-coenzyme A and oxaloacetate from citrate and coenzyme A (CoA).

109 phorylation), substrate channeling (e.g., of oxaloacetate from malate dehydrogenase to citrate syntha

110 The diffusion of oxaloacetate from one of the active sites of malate dehy

111 ial pyruvate metabolism; (e) the transfer of oxaloacetate from the cytosol to the mitochondria is lar

112 the substrate, fumarate, and the inhibitors oxaloacetate, glutarate, and 3-nitropropionate.

113 on the activities of MDH is unidirectional (oxaloacetate --> malate).

114 ed, and that for the physiological substrate oxaloacetate has been diminished, through the replacemen

115 ces the rate of enzymatic decarboxylation of oxaloacetate in the carboxyl transferase domain of pyruv

116 etyl-CoA (3 micromol/l), which combines with oxaloacetate in the citrate synthase reaction and lowers

117 Channeling of oxaloacetate in the malate dehydrogenase and citrate syn

118 talysis of the interconversion of malate and oxaloacetate in the tricarboxylic acid cycle.

119 ow that the glucose metabolites pyruvate and oxaloacetate inactivate HIF-1alpha decay in a manner sel

120 K(m) values for oxaloacetate increased 2-2.8-fold.

121 The high reactivity of PFR1 toward oxaloacetate indicates that in vivo, fermentation might

122 AP as well as glyceraldehyde 3-phosphate and oxaloacetate inhibited activity of both yeast and human

123 Oxaloacetate inhibits E1o activity at physiological conc

124 The channeling of the oxaloacetate intermediate was the same for the porcine f

125 ools through the conversion of mitochondrial oxaloacetate into phosphoenolpyruvate.

126 Oxaloacetate is also a substrate for HCS, but with lower

127 Synthesis of phosphoenolpyruvate (PEP) from oxaloacetate is an absolute requirement for gluconeogene

128 d (ii) intrinsic inhibition of Complex II by oxaloacetate is an inherent mechanism that protects agai

129 e presence of oxamate, the apparent K(m) for oxaloacetate is artificially suppressed (from 15 to 4-5

130 Subsequently, this oxaloacetate is converted into malate and then pyruvate,

131 ation rate, which suggests that an excess of oxaloacetate is converted to aspartate and reintroduced

132 The results show that oxaloacetate is not transferred directly from AATase to

133 H) catalyzed oxidation/reduction of L-malate/oxaloacetate is pH-dependent due to the proton generated

134 In bacteria, oxaloacetate is subject to enzymatic decarboxylation; ho

135 lycolysis; (c) the majority of the cytosolic oxaloacetate is synthesized via anaplerotic carboxylatio

136 random, suggesting that the enol tautomer of oxaloacetate is the product; this expectation was confir

137 to the discovery that PA4872 decarboxylates oxaloacetate (kcat = 7500 s(-1) and Km = 2.2 mM) and 3-m

138 the enzyme first, followed by the binding of oxaloacetate/L-malate.

139 dentified (2R)-ethyl, (3S)-methylmalate, and oxaloacetate [likely to bind as the hydrate, C(2)(OH)(2)

140 tabolic end products of lignin (pyruvate and oxaloacetate) must enter the tricarboxylic acid (TCA) cy

141 To test how one intermediate, oxaloacetate (OAA) affects brain bioenergetics, insulin

142 he crystal structure of AaCS, complexed with oxaloacetate (OAA) and the inhibitor carboxymethyldethia

143 recognition of phosphoenolpyruvate (PEP) and oxaloacetate (OAA) by cytosolic phosphoenolpyruvate carb

144 -oxidation (redox) homeostasis is the malate-oxaloacetate (OAA) shuttle.

145 y intriguing condensation of acetyl-CoA with oxaloacetate (OAA) to form citryl-CoA and the subsequent

146 uconeogenesis by conversion of mitochondrial oxaloacetate (OAA) to phosphoenolpyruvate, regulates glu

147 Oxaloacetate (OAA), pyruvate, and glutarate behave as de

148 mation of the binary complex with substrate, oxaloacetate (OAA).

149 ner, and recuperates photorespiratory CO2 as oxaloacetate (OAA).

150 H274G cannot properly activate either oxaloacetate or acetyl-coenzyme A, and the condensation

151 gative charge of the substrate side-chain of oxaloacetate or alpha-ketomalonate, charge repulsion wou

152 he ketimine of pyridoxamine 5'-phosphate and oxaloacetate or pyruvate.

153 talysis, such as the conversion of malate to oxaloacetate or the activation of the toxin 3-nitropropi

154 and bound to its substrate pyruvate, product oxaloacetate, or inhibitor 2-ketobutyrate.

155 r via measurement of D(V/K), T(V/K), and the oxaloacetate partition ratio.

156 zes of amino acids derived from pyruvate and oxaloacetate, polyamine precursors, and compatible solut

157 pathways, including pyruvate, glutamate and oxaloacetate pools, and urea production from arginine, w

158 PpcA was the only recognizable oxaloacetate-producing enzyme in Methanopyrus kandleri,

159 analyses with succinate, fumarate, L-malate, oxaloacetate, pyruvate and D- and L-2HG support the kine

160 ate, succinate, 2-hydroxyglutarate, citrate, oxaloacetate, pyruvate, isocitrate, and lactate using a

161 sparagine), yielding alpha-ketoglutarate and oxaloacetate, respectively.

162 catalyzes the readily reversible reaction of oxaloacetate reversible malate using either NADH or NADP

163 In catalysis, oxaloacetate serves as a nucleophile by forming an enola

164 that in glucose grown cells, both the malate/oxaloacetate shuttle and a glycerol-3-phosphate dehydrog

165 are transferred to the cytosol by the malate/oxaloacetate shuttle.

166 The results with the series of oxaloacetate site mutants, H320X, strongly suggest that

167 vely, and (3) reactant (malate) and product (oxaloacetate) states that are nearly isoenergetic.

168 es identified in PCS, destabilization of the oxaloacetate substrate carbonyl and stabilization of the

169 ained decarboxylase activity for the smaller oxaloacetate substrate, which is not inhibited by excess

170 Burkholderia species utilize acetyl-CoA and oxaloacetate, substrates for citrate synthase in the TCA

171 decrease in malic acid, and lower amounts of oxaloacetate, suggesting that malate metabolism plays an

172 In Methanothermobacter thermautotrophicus, oxaloacetate synthesis is a major and essential CO(2)-fi

173 is presented that, in Methanosarcina barkeri oxaloacetate synthesis, an essential and major CO(2) fix

174 1) For oxaloacetate synthesis, V(max) decreased 1.4-4-fold.

175 provision for pyruvate carboxylase-mediated oxaloacetate synthesis.

176 results and the existence of an alternative oxaloacetate synthesizing enzyme phosphoenolpyruvate car

177 This methanogenic archaeon possesses two oxaloacetate-synthesizing enzymes, pyruvate carboxylase

178 ctivity, but with K(m) values for malate and oxaloacetate that are surprisingly unaffected.

179 tenuated by addition of the energy substrate oxaloacetate, the activator of pyruvate dehydrogenase, d

180 For producing oxaloacetate, the enzyme utilized both GDP and IDP; ADP

181 gATP, the oxamate-induced decarboxylation of oxaloacetate, the phosphorylation of MgADP by carbamoyl

182 determined for the forward reaction to form oxaloacetate, the reverse reaction to form MgATP, the ox

183 sion reduced TCA cycle activity and diverted oxaloacetate, the substrate of CS, into production of th

184 gest that activation of the first substrate, oxaloacetate, through carbonyl bond polarization, not ju

185 ase reaction and lowers the concentration of oxaloacetate, thus beginning another oscillation.

186 th the portion of the citric acid cycle from oxaloacetate to alpha-ketoglutarate via cis-aconitate.

187 glutarate with the concomitant conversion of oxaloacetate to aspartate.

188 e base, its failure to further condense with oxaloacetate to form a sulfur-less analog of citryl-coen

189 sible decarboxylation and phosphorylation of oxaloacetate to form phosphoenolpyruvate.

190 (PEPCK), forward TCA cycle flux of [4-(13)C]oxaloacetate to generate (13)CO(2) at isocitrate dehydro

191 rease the rate of the coupled L-aspartate to oxaloacetate to malate sequence only if the direct metab

192 f the enzymatic conversion of hyperpolarized oxaloacetate to malate, the two signal components are se

193 atalyzed by pyruvate carboxylase will supply oxaloacetate to mitochondrial aspartate aminotransferase

194 te and subsequent transfer of (13)CO(2) from oxaloacetate to oxamate.

195 provides the acetyl-CoA that condenses with oxaloacetate to support citrate production.

196 The diet may limit the availability of oxaloacetate to the aspartate aminotransferase reaction,

197 can be productively metabolized by glutamate oxaloacetate transaminase (GOT) to maintain cellular ene

198 s work demonstrated the ability of glutamate oxaloacetate transaminase (GOT) to metabolize neurotoxic

199 uced significant increase in serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruva

200 gage the neuroprotective effect of glutamate oxaloacetate transaminase against stroke.

201 C-transformed cells depend on both glutamate-oxaloacetate transaminase and glutamate dehydrogenase to

202 Glutamate oxaloacetate transaminase enables anaplerotic refilling

203 e, DR, which is closely related to glutamate-oxaloacetate transaminase, EC 2.6.1.1.

204 to the mitochondria and cytoplasm, glutamate oxaloacetate transaminases (GOT), and malate dehydrogena

205 ansported via malate, which when oxidized to oxaloacetate, transfers an electron pair to reduce NAD t

206 t understanding would not have predicted the oxaloacetate transforming activity of Ser101Leu102 or th

207 ing cataplerotic decarboxylation of [4-(13)C]oxaloacetate via phosphoenolpyruvate carboxykinase (PEPC

208 se (DET0724-0727) and pyruvate conversion to oxaloacetate via pyruvate carboxylase (DET0119-0120).

209 arboxylation was suppressed, and anaplerotic oxaloacetate was derived from glutamine.

210 An unexpected binding mode for oxaloacetate was observed in which it coordinates the ac

211 bstrate analog: the gem-diol of 3,3-difluoro-oxaloacetate) was determined for the purpose of identify

212 e, as competing enzymes for the intermediate oxaloacetate, was examined.

213 protein could account for the channeling of oxaloacetate we observed with the yeast fusion protein.

214 late through protocatechuate to pyruvate and oxaloacetate were demonstrated in cells or cell extracts

215 oglutarate, succinate, fumarate, malate, and oxaloacetate) were tested for their influence on AOX1A,

216 boxylase were less effective competitors for oxaloacetate when precipitated citrate synthase and mito

217 rate of decarboxylation of the intermediate oxaloacetate which occurs at 11 s-1.

218 liberated enzymatically by transamination to oxaloacetate with aspartate aminotransferase and then de

219 Substrate channeling of oxaloacetate with citrate synthase-mitochondrial malate