ライフサイエンスコーパス: pH dependence

1 M is dominated by one conformation with weak pH dependence.

2 the urease reaction with a bell-shaped rate-pH dependence.

3 to those of the native pigment exhibiting no pH dependence.

4 stimulation, and (as revealed in this study) pH dependence.

5 parable to those of the wt in efficiency and pH dependence.

6 actions at this position and/or change their pH dependence.

7 favored at lower pH, whereas the DM shows no pH dependence.

8 c activity, substrate affinity, and reaction pH dependence.

9 oxidation of phen by PMS features a complex pH dependence.

10 however, His378Asn/Arg variants show no such pH dependence.

11 t qualitatively agrees with the experimental pH dependence.

12 periments revealed three distinct regions of pH dependence.

13 ted mutagenesis indeed eliminated the strong pH dependence.

14 147) to Val, which had minimal effect on the pH dependence.

15 onohydrogen-phosphate, as can be seen by the pH dependence.

16 approximately 500-fold, with maintenance of pH dependence.

17 rption capacity, which demonstrated the same pH dependence.

18 hich increases kcat 23-fold and restores its pH dependence.

19 results of metal alteration, mutations, and pH dependence.

20 arrier for the rate-determining step and its pH dependence.

21 y residues in other proteins causing similar pH dependence.

22 electron transfer as well as changes in its pH dependence.

23 s of the wild-type and mutant VCPO and their pH-dependence.

24 experiments were small and showed no notable pH-dependence.

25 xhibiting greater longevity and a pronounced pH-dependence.

26 ere incorporated into a kinetic model of the pH dependence and compared to a pseudo-steady-state mode

27 of Ca2+ to its effector site eliminates this pH dependence and locks both g(eff) and A(Z) at values o

28 dues are likely the structural basis for the pH dependence and sensitivity to ionic strength of influ

29 The pH dependence and thermostability reveal that the enzyme

30 id residues suggested that the face-specific pH dependences and Na(+)/K(+) selectivities may arise fr

31 es, an increased affinity for ATP, different pH dependence, and a decreased sensitivity to azide inhi

32 ility of ZIP8, its subcellular localization, pH dependence, and regulation by iron.

33 On the basis of kinetic properties, pH dependence, and structural information, we propose an

34 copic ligand-receptor binding species, their pH dependence, and their contributions to the phenomenol

35 This observed pH-dependence appears to result from photooxidants react

36 enomenological binding affinities, and their pH dependence are all in good agreement with available e

37 stabilization was consistent with the strong pH dependence arising from the GKD tripeptide unit.

38 His51 cannot account for the observed pH dependence as neutral amino acid substitutions failed

39 exhibit the same kinetic behaviors (KIE and pH dependence) as the catalyzed chemical reaction.

40 The pH dependence, as determined from the primary isotope ef

41 The midpoint potential displays a distinct pH dependence at acidic pH values, indicative of proton-

42 a I(480) decay did not display the anomalous pH dependence characteristic of classical Meta II in the

43 (I) affinity of WT HAH1 exhibits a different pH-dependence compared to MNK1 and Lys60Ala HAH1.

44 rotein stability, the hydrogen bond network, pH dependence, conformational dynamics and protein funct

45 mutated to histidine, the enzyme develops a pH dependence corresponding to a loss of catalytic power

46 nd equilibrium experiments suggests that the pH dependences determining membrane association and tran

47 ble anodic photocurrent whose potential- and pH-dependences exhibit broad applicability.

48 The lack of pH dependence explains in part why NP4 cannot use the fe

49 ve distinct trafficking routes, we suggest a pH dependence for multiple cargo sorting events at the G

50 his hypothesis is examined in the context of pH dependence for other members of the Kv1 family, and m

51 revealed the structural determinants of the pH dependence for the interaction of these inhibitors wi

52 The disparate pH dependences for reactivation of ChE and the general b

53 for trace Tl adsorption indicated a moderate pH-dependence from pH 2.5 to 11.

54 This pH dependence has been one of the major disadvantages th

55 The observed speed-pH dependence holds promise for sensitive pH measurement

56 The pH dependence, hydrogen/deuterium (H/D) isotope effect,

57 Steady-state kinetics exhibited a pH dependence in k(cat), which prompted us to elucidate

58 ion shifts at multiple sites, increasing the pH dependence in the mutant.

59 The FHA-FBM interactions exhibit significant pH dependence in the physiological range as a consequenc

60 acid (DMAPA, azinoyl = N(+)(O(-)) has a weak pH-dependence in D(2)O, with a slight preference for tra

61 There was a pH-dependence in the reduction of NO2(-) by magnetite; t

62 th a rate constant that showed a bell-shaped pH dependence indicative of participation of factor Xa a

63 onic stress in erythrocyte membranes had the pH dependence, ion dependence, and inhibitor sensitivity

64 gher pH, the functional significance of this pH dependence is unknown.

65 kcat 10(3.4)-fold and largely abolishes its pH dependence, is rescued by the Y103F mutation, which i

66 The absorption spectrum also exhibits a pH dependence larger than any other retinal protein.

67 , dark background, facile synthesis, and low pH dependence make NO(550) a superior probe for NO detec

68 n-hydroxyl co-adsorption causes the apparent pH dependence of "hydrogen" adsorption in the step sites

69 Although we earlier had observed the pH dependence of a (15)N NMR signal from each of the two

70 The Mn(II) concentration and pH dependence of a preceding lag phase indicates weak Mn

71 GMQ shifts the pH dependence of activation to more acidic pH in ASIC1a

72 ear single quantum coherence analysis of the pH dependence of amide chemical shifts in fragment C37 w

73 ombination of the ionic-strength effect, the pH dependence of anion adsorption, and the competition b

74 a surface complexation model describing the pH dependence of As(III) binding to the organic adsorben

75 The 5b molecule not only shifts pH dependence of ASIC1a activation but also inhibits its

76 The pH dependence of assembly kinetics and extent of assembl

77 Analysis of the pH dependence of assigned (13)C', (13)C(alpha), and (15)

78 The pH dependence of binding had an apparent pKa of 7.2, a v

79 The pH dependence of binding reveals a pK(a) of 6.7, suggest

80 The pH dependence of binding, obtained by nuclear magnetic r

81 A comparison of the pH dependence of both kred and kred/Kd from reductive ha

82 The impact of AdoMet binding and the pH dependence of catalysis are also quantitatively obser

83 The pH dependence of catalysis, the turnover frequency, and

84 Combination of acidity measurements with the pH dependence of catalytic rates unequivocally shows tha

85 The pH dependence of cell-expressed mutants (L1C and L2 swap

86 tral binding site) reduced also the internal pH dependence of ClC-5.

87 asparagines exhibits a much less pronounced pH dependence of collagen binding.

88 It is known that the pH dependence of conductance for the rat potassium chann

89 e of Molecular Microbiology, analyses of the pH dependence of cytoplasmic [Na(+)], [K(+)], pH and tra

90 We were also able to model the pH dependence of diffraction of the HEMA PCCA by using F

91 The pH dependence of digestion patterns shows that, in the p

92 hFc with the capture surface, influences the pH dependence of dissociation from the capture surface.

93 The pH dependence of enzyme activity and inhibition has been

94 , these variants showed a markedly different pH dependence of fibril formation versus that of PAPf39.

95 ough apparent pKa values determined from the pH dependence of fluorescence intensity shift in the bas

96 onomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightnes

97 H170 interaction in dimer stability and the pH dependence of fusion and provide evidence for stepwis

98 studies, mass spectrometry studies, and the pH dependence of guest encapsulation.

99 Alternative explanations for the pH dependence of hairpin ribozyme reactivity are discuss

100 -Changeux allosteric model revealed that the pH dependence of Hb-O(2) affinity stems primarily from c

101 take is more flexible as it accounts for the pH dependence of HONO uptake and bulk diffusion in the s

102 zation mass spectrometry (CIMS) to study the pH dependence of HONO uptake onto agricultural soil and

103 also present an initial investigation of the pH dependence of hPRL function in rat Nb2 cell prolifera

104 GMQ also induces an acidic shift of the pH dependence of inactivation of ASIC1a, -1b, -2a, and -

105 The pH dependence of inhibition (K(ii)), on the contrary, in

106 state and pre-steady-state kinetics, and the pH dependence of inhibition and binding.

107 ions tested; however, the measurement of the pH dependence of iodoacetamide alkylation revealed a pK

108 f ICRAC on intracellular pH strictly follows pH dependence of iPLA2beta activity, and 7) (S)-BEL, a c

109 Consistent with the pH dependence of iron binding, the main trigger for iron

110 By analyzing the pH dependence of isomerization and protonation equilibri

111 he pKa of 2 was estimated to be 6.9 from the pH dependence of its ring-closing to the pyrimidopurinon

112 operties of hPRL, we have noted a surprising pH dependence of its structural stability over a range f

113 The pH dependence of IYD binding and turnover also supports

114 The pH dependence of k cat for E155A in the presence of form

115 d 1.8 on V/ K AcCoA and V, respectively; the pH dependence of k cat was similar to that of the wt.

116 In contrast, the pH dependence of k(cat) values indicates a much lower ap

117 For the manganese substituted BcII, the pH dependence of k(cat)/K(m) for benzylpenicillin, cepha

118 The pH dependence of Kb revealed that the RA carboxylic form

119 Measurement of the pH dependence of kcat and kcat/Km values for both wild-t

120 The pH dependence of kcat/Km and of kcat for the wild-type P

121 The pH dependence of KCC in "euvolemic" SS RBCs treated with

122 euterium isotope effects, and changes in the pH dependence of kinetic parameters compared to that of

123 The pH dependence of kinetic parameters suggests that the hy

124 sm for HIcDH is proposed on the basis of the pH dependence of kinetic parameters, dissociation consta

125 al mechanism is proposed on the basis of the pH dependence of kinetic parameters, dissociation consta

126 Analysis of the pH dependence of L-glutamate stereoisomerization suggest

127 Kinetic analyses show a pH dependence of ligand binding that is consistent with

128 The pH dependence of lysozyme activity arises not from chang

129 This pH dependence of metal ion binding suggests that the loc

130 The pH dependence of metalation under physiological conditio

131 From the pH dependence of methyl chemical shifts, a pKa of 7.7 is

132 ersion, we have measured the temperature and pH dependence of microsecond to millisecond time scale b

133 a tautomeric or ionized base pair, with the pH dependence of misinsertion consistent with the latter

134 more His residues (pK(a) ~6.0) mediates the pH dependence of multimerization.

135 found to be responsible for controlling the pH dependence of NAADP synthesis by the cyclase.

136 anism by which these mutations influence the pH dependence of NhaA, the substrate dependence of the k

137 A conceptual model describing the pH dependence of PAPf39 aggregation is proposed and prov

138 ssary for fibril formation but modulates the pH dependence of PAPf39 fibril formation.

139 To do so we took advantage of the pH dependence of parallel Hoogsteen interactions and rat

140 The bell-shaped pH dependence of permeability suggests that, in addition

141 The single turnover pH dependence of pre-tRNA cleavage revealed a single ion

142 length PA83 protein in the pH 5-6 range, the pH dependence of prepore-to-pore conversion of (PA63)7 w

143 The pH dependence of protein stability calculated from NMR-d

144 mine the unfolded state pK(a) values and the pH dependence of protein stability for this enzyme.

145 Analysis of the pH dependence of quasi-reversible features in cyclic vol

146 The trend of pH dependence of rates for Trp (i.e., aromatic alpha-ami

147 The pH dependence of reduction was also determined, and a pK

148 The pH dependence of seHAS in the presence of synthetic or n

149 The pH dependence of silicate sorption exhibited a maximum b

150 The model calculations reveal a strong pH dependence of solubility, typically controlled by the

151 oncentrations at varying pH to establish the pH dependence of sorption.

152 Last, the pH dependence of SrtA inactivation by iodoacetamide reve

153 The mutants D302A and H305A remove the pH dependence of stability.

154 ate-inhibited, but unlike the H287C variant, pH dependence of substrate inhibition was largely elimin

155 the site of protonation responsible for the pH dependence of sugar binding.

156 A breakpoint in the pH dependence of the 2'-O-transphosphorylation rate to a

157 We have measured and analyzed the pH dependence of the 695 nm charge transfer band of hors

158 The binding-linked protonation and pH dependence of the association constant (Kb) for the e

159 data by QM/MM calculations suggest that the pH dependence of the binding of NO, but not of CN(-) and

160 The pH dependence of the catalytic activity also implicates

161 site-directed mutagenesis, evaluation of the pH dependence of the catalytic efficiency (V(max)/K(M)),

162 in the pili, as well as the temperature and pH dependence of the charge density, were similar to tho

163 e and found a strong correlation between the pH dependence of the cleavage reaction and the intrinsic

164 The pH dependence of the competitive inhibition constant, K(

165 Based upon the observed pH dependence of the current amplitude and oxidation/red

166 studied the external and internal Cl(-) and pH dependence of the currents of E268A.

167 substrates was investigated by measuring the pH dependence of the D(kcat/KNE) value with 1,1-[2H2]-ni

168 recognition helix accounted for most of the pH dependence of the DNA binding.

169 sfer processes and can be interpreted by the pH dependence of the emitted light.

170 e binding and catalysis without changing the pH dependence of the enzyme.

171 te similar to that obtained by analyzing the pH dependence of the epsilon(240) of wild-type AhpC (5.8

172 The pH dependence of the F3Y122* left arrow over right arrow

173 The pH dependence of the fast phase kinetic data shows that

174 The pH dependence of the first-order rate constant for decay

175 The rate's pH sensitivity reflects the pH dependence of the four-electron O2-H2O couple.

176 The pH dependence of the free energy of denaturation was des

177 Likewise, studies of the pH dependence of the histidine substitution indicate a s

178 We have found that the pH dependence of the hydrolysis reaction is log-linear,

179 The pH dependence of the IrOx open circuit potential (OCP) w

180 The pH dependence of the kcat/Km and kcat values with betain

181 ng catalysis was examined by determining the pH dependence of the kcat/Km values with ethylnitronate

182 The pH dependence of the Ki for glyoxylate, a competitive in

183 The pH dependence of the kinetic isotope effects with [1,1-(

184 The pH dependence of the kinetic parameters revealed that th

185 The pH dependence of the kinetic parameters reveals that one

186 The pH dependence of the kinetic parameters was determined t

187 The pH dependence of the kinetic parameters with the WT enzy

188 On the basis of kinetic properties, the pH dependence of the kinetic parameters, and structural

189 In this report, we have determined the pH dependence of the kinetic parameters, revealing that

190 The pH dependence of the kinetics was found to be fully cons

191 duced and quantified the previously observed pH dependence of the major conformation of Lys48-linked

192 A detailed study of the pH dependence of the midpoint potential of skeletal hors

193 Here we examine the pH dependence of the monomer-dimer-tetramer reaction, of

194 The pH dependence of the nonenzymatic pathway for conversion

195 The calculated strong pH dependence of the number of chlorides bound and the a

196 d with dried buffer that prevents any sample pH dependence of the observed color change.

197 The pH dependence of the observed rate constants for O-acyli

198 The pH dependence of the on- and off-rate constants for suga

199 esulting acid triad is central to the marked pH dependence of the optical-absorption relaxation kinet

200 sfully reproduce the experimentally observed pH dependence of the overall rate and H/D kinetic isotop

201 The experiments revealed a strong pH dependence of the oxidation of ACV and carboxy-ACV wi

202 ion being the key species accounting for the pH dependence of the oxidation.

203 penetration of the blood-brain barrier, the pH dependence of the oxime and amine ionizing groups of

204 The pH dependence of the PHM-catalyzed monooxygenation of da

205 The difference in pH dependence of the pKESB for cysteine and serine, the

206 dditionally, the structural models also show pH dependence of the protein structure itself, which pro

207 The zeta-potential/pH dependence of the Pu(IV) colloids is similar to that

208 Analysis of the pH dependence of the rate constant for tyrosyl radical g

209 The pH dependence of the rate constants, the correlation bet

210 -base catalysis, and this in accord with the pH dependence of the reaction rate for the natural and m

211 nism is also consistent with the bell-shaped pH dependence of the reaction rate.

212 A nonmonotonic super-Nernstian pH dependence of the redox peaks with increasing Fe cont

213 The pH dependence of the reduction potential E degrees for a

214 The pH dependence of the second-order rate constant k cat/ K

215 The pH dependence of the second-order rate constants of inhi

216 e stability of the autoinhibited complex and pH dependence of the secondary cleavage provide means fo

217 The pH dependence of the solid-state (67)Zn NMR lineshapes h

218 Additionally, the pH dependence of the solution and enzymatic reactions pr

219 Although H290 is important for pH dependence of the stability, it is not critical for d

220 sis of these isotope effects, along with the pH dependence of the steady-state kinetic parameters, li

221 Global analysis of the pH dependence of the trimer-dodecamer equilibrium reveal

222 The pH dependence of the uptake of [(3)H]-methyltetrahydrofo

223 The pH dependence of the water splitting reaction suggests a

224 We determined the pH dependence of these bimolecular rate constants and fo

225 Herein, we report the pH dependence of these tautomeric forms providing the fi

226 To unlock the pH dependence of these transient states with atomistic-l

227 fferent from wild-type, and furthermore, the pH dependence of this potential is not the same as for w

228 of conserved aspartate residues explains the pH dependence of this transition, and biochemical studie

229 The pH dependence of Trp-245 unquenching exhibits a pK(a) of

230 The pH dependence of V(max) for both glutamate variants yiel

231 and showed modest change or no change in the pH dependence of virus-membrane fusion.

232 The pH dependence of Vmax for Arg83 and Asn328 mutants is si

233 The pH dependence of Vmax for wild-type DmTrxR has pKa value

234 We have previously attributed the pH dependence of Vmax to the deprotonation of the metal-

235 ines to alanine residues did not abolish the pH dependence of ZnR/GPR39.

236 Analysis of the pH dependences of the gating and open channel parameters

237 The pH-dependence of backbone amide NMR resonances demonstra

238 folding led to apparent cooperativity in the pH-dependence of folding, although each group titrated i

239 The pH-dependence of HWKK binding to polyU provides no evide

240 The pH-dependence of log 1/K(M) is a sigmoidal curve reachin

241 Substantial pH-dependence of maturation was observed upon substituti

242 ase pairing, was confirmed by studies of the pH-dependence of mismatching.

243 Through these structural effects and the pH-dependence of Mn(II)-metal competitive adsorption, sy

244 w the PA:receptor interaction influences the pH-dependence of pore formation; and how the pore functi

245 These results demonstrate that the acid pH-dependence of protein stability in these hyperthermop

246 e overcome this challenge by quantifying the pH-dependence of quinary interactions in living Escheric

247 The simple DLM describes well the pH-dependence of single adsorption edges.

248 optimized biochemical methods, we dissected pH-dependence of spontaneous and DM-DO-mediated class II

249 es in low salt, indicating that the observed pH-dependence of stability was primarily due to these th

250 take/release and thus resulting in different pH-dependence of the binding energy.

251 The pH-dependence of the catalytic parameters was measured u

252 etermining the substrate specificity and the pH-dependence of the catalytic rates, is poorly understo

253 The results indicate pH-dependence of the fumarate modulation with opposite b

254 The pH-dependence of the pre-steady-state phosphorylation of

255 of the C41 base triple to the metal ion- and pH-dependence of the reaction are examined.

256 t this site II, as well as at site I, confer pH dependence on HN's receptor avidity.

257 The pH dependence on the kinetic constants suggests that a s

258 Further studies of the pH dependence on the observed rectification support a su

259 es, suggesting that multiple residues confer pH dependence on the transport mechanism.

260 a function of extracellular solution pH, the pH-dependence on Mg isotope fractionation is thus due to

261 Model analysis suggests that the pH-dependence on turnover is primarily due to the pKa va

262 hylene shorter (Orn) resulted in significant pH dependence or lack of interaction, suggesting that na

263 This favorable pH dependence originates from several elements of struct

264 n the switches, we can rationally tune their pH dependence over more than 5 pH units.

265 Ca2+, the EPR-active Mn3+ exhibits a strong pH dependence (pH approximately 6.5-9) of its ligand-fie

266 However, their differences in pH dependence, phosphate and fluoride inhibition pattern

267 ty to design DNA-based switches with tunable pH dependence provides the opportunity to engineer pH na

268 ith the charge recombination process shows a pH dependence ranging from 2.3 eV at pH </= 7 and 1.2 eV

269 The near absence of an intrinsic pH dependence represents an advantage compared to hydrog

270 ts transport of riboflavin to have an acidic pH dependence, saturability (apparent Km = 1.4 +/- 0.5 m

271 Analysis of binding coefficients and pH dependence showed that (125)I-STa-binding in GC-C KO

272 Kinetic analyses and pH dependence studies of the wild type and variant enzym

273 pH dependence studies revealed no inflection points in t

274 pH dependence studies show that the reaction rate increa

275 In addition, pH dependence studies show that the relative abundance o

276 and Asn593, and kinetic isotope effects and pH-dependence studies of the wild-type enzyme.

277 A pH dependence study allowed the determination of the pK(

278 The EPR spectrum and its pH dependence suggest that the coordination environment

279 We found that H216 is responsible for this pH dependence, suggesting that protonation of H216 could

280 The pH dependence suggests that the active form is the neutr

281 This novel pH dependence suggests that the hydrolysis is determined

282 ics of imatinib binding to Abl kinase have a pH dependence that disappears when the DFG aspartate is

283 e concentration of the peptide, also shows a pH dependence that is described and discussed.

284 excited state by dissolved oxygen exhibits a pH dependence that parallels that of the excited state l

285 e voltages of the transistors demonstrated a pH-dependence that was linear over a wide range and coul

286 charge movements within the PR and shift its pH dependence to acidic values.

287 red to bind with higher affinity and reduced pH dependence to FcRn potently inhibits FcRn-IgG interac

288 lecular fragments narrows the source of this pH dependence to its N-methylthiazolium fragment.

289 Thermodynamic modeling of the pH dependence to receptor binding affinity reveals large

290 for inactivation, k(i), both show a similar pH dependence to that exhibited by the beta-lactamase-ca

291 e investigated the mechanism underlying this pH dependence using a combined approach of site-directed

292 ) and pH(i) independently revealed that this pH dependence was entirely due to effects of pH(i) and t

293 The pH dependence was found to be dominated by k(cat), with

294 The origin of the observed pH dependence was further narrowed to the protonation eq

295 Lead uptake was larger and pH dependence was greater for the (012) and (110) surfac

296 the temperature, concentration, solvent, and pH dependence, we extract a thermodynamic and kinetic ch

297 rt to explain the microscopic origin of this pH dependence, we studied Abeta11-25 fibrils using metho

298 sidues that are likely to play a role in the pH dependence were identified.

299 Na(+)/H(+) antiporters show a marked pH dependence, which is important for their physiologica

300 A/(k(cat)/KM)LA ratio was observed to have a pH dependence, which was fit with a titration curve (pKa