ライフサイエンスコーパス: panning

1 purified from FVB/N mouse stomachs by lectin panning.

2 t sufficiently high to allow purification by panning.

3 raries of rANP-display phage by differential panning.

4 d a 195-fold enrichment after five cycles of panning.

5 ide Y, following screening by solution phage panning.

6 imized by using phage display and cell-based panning.

7 specificity of the interaction identified by panning.

8 ive antigens after as few as three rounds of panning.

9 After three rounds of subtractive panning, 239 of 673 clones analyzed bound selectively to

10 Nipah virus (NiV) and Hendra virus (HeV) by panning a large nonimmune antibody library against a sol

11 anti-Akt1 single-chain antibodies (scFv) by panning a mouse phage-displayed scFv recombinant antibod

12 isplay format and make it ideally suited for panning a wide variety of target antigens.

13 From this panning, a C-terminal fragment of FGF-BP1 (amino acids 1

14 r muscle-targeting capsids by direct in vivo panning after tail vein injection in mice.

15 ainst fresh neutrophils and independently by panning against a fixed cell line.

16 Panning against bovine trypsin resulted in enrichment of

17 Phage were selected by panning against digoxin, gitoxin (16-OH), and 16-acetylg

18 able fragments (scFvs) after three rounds of panning against FAP.

19 high-affinity human mAb, ANA15, selected by panning against fresh neutrophils and independently by p

20 ide phage display libraries using whole-cell panning against human melanoma cell line Me6652/4.

21 (scFv) phage-display library was used in bio-panning against live, native spores of Bacillus subtilis

22 s from peptide-presenting phage libraries by panning against primary patient CLL cancer cells.

23 Panning against rat trypsin resulted in enrichment as we

24 Panning against rat trypsin resulted in enrichment but n

25 In contrast, panning against secreted EBO glycoprotein (SGP) resulted

26 nesis of its heavy-chain variable domain and panning against sGHeV.

27 0 cDNA-bacteriophage library was enriched by panning against solid- and solution-phase PAI-1.

28 cted from a human nonimmune phage library by panning against the chimeric construct N(CCG)-gp41 (whic

29 specific cPTMs are significantly enriched in panning against the specific targets.

30 ry binding site of ecotin, was generated for panning against uPA and rat trypsin.

31 Panning against uPA resulted in enrichment as well as th

32 methodological innovation, termed iterative panning and blocking (IPAB), to extend the range of spec

33 the surface of 1264 and BEAS cells used for panning and counter-selection was estimated as 75,000 +/

34 A direct in vitro panning and enzyme-linked immunosorbent assay-based sele

35 d signaling networks with dynamical zooming, panning and linking capabilities.

36 s that could be missed by conventional phage panning and screening methods.

37 y bound or eluted phage at various stages of panning and screening procedures.

38 We use a solution-based panning and screening strategy conducted in the presence

39 Repeated panning and selection experiments against a 15-mer pepti

40 ystin LR and its selection using competitive panning and two novel panning strategies.

41 SurfaceSlide users can perform direct panning and zooming operations on digitised slide images

42 was provisionally mapped by peptide display panning, and a peptide sequence matching the epitope was

43 addition, offer the flexibility of zooming, panning, and being adjustable for slow image acquisition

44 lly support Tat transactivation was used for panning, and of the five unique anti-hCyclinT1 sFvs that

45 Here we applied an in vitro whole cell panning approach using antibody phage display technology

46 Through a whole-cell panning approach, we previously identified a panel of an

47 By this "epitope-blocked panning" approach, two novel Fabs, encoded by unique VH

48 an efficiency that exceeds surface-confined panning approaches; and (3) the co-detection of ssDNAs,

49 rovide a intuitive interface for zooming and panning around digital images.

50 Thus, phage library panning combined with expression cloning permits identif

51 selection by tailoring the stringency of the panning conditions.

52 Here, phage-peptide library panning coupled with screening using next generation seq

53 the antigenic profile of the cells used for panning determines the specificity of the preponderant p

54 Phage recovered from this second panning exhibited a motif (Ser-His-Tyr) that was subsequ

55 Using M13qPCR in phage-panning experiments on live leukemia and prostate cancer

56 lection of this peptide in multiple reported panning experiments.

57 ns to be propagated as either phage for more panning from repertoires or expressed as soluble sdAb fo

58 After five rounds of panning, individual positive scFv clones were used to in

59 ii) selected functional recombinant phage by panning; (iv) purified the ScFv peptide; (v) shown that

60 ociated retinal cells by a modified two-step panning method and were cultured in serum-free medium co

61 Here, we use a novel cysteine panning method to identify the second cleavage site (Sit

62 ostnatal days 7 and 8 by a modified two-step panning method.

63 may not be accessible by conventional B cell panning methods.

64 through a phage display library and by (ii) panning multiple libraries encoding structurally distinc

65 phage-displayed heavy chain-only antibody by panning of a large (size, approximately 1.5x10(10)) huma

66 dra G (sG) which was used as the antigen for panning of a large naive human antibody library.

67 Affinity panning of a library of humanized A4.6.1 antibody mutant

68 dy FC5 was previously isolated by phenotypic panning of a naive llama single-domain antibody phage di

69 in soluble CD4 (sCD4) were alternated during panning of a phage-displayed human antibody library; the

70 G(M3)-derived reagents and their use in the panning of a phage-displayed human single-chain Fv (scFv

71 tence of such a motif was established by the panning of a random peptide library in which peptide seq

72 ssive molecule CD200 were identified by cell panning of an antibody phage display library derived fro

73 Thus, direct in vivo panning of capsid libraries is a simple tool for the de-

74 Panning of IEs on HBMECs led to a more dispersed binding

75 motifs that bind to GroEL by using affinity panning of immobilised GroEL minichaperones for a librar

76 er of structure-based design with sequential panning of large D1 mutant libraries against different H

77 Affinity panning of large libraries is a powerful tool to identif

78 Panning of phage display peptide libraries with mAb 763.

79 Panning of phage Fab libraries against purified antigens

80 regions of arrestin that bind to rhodopsin: panning of phage-displayed arrestin fragments against RP

81 Panning of such libraries enriched strongly peptides wit

82 Panning of the hot spot libraries yielded several mutant

83 Initial panning of the library isolated only non-pathogenic scFv

84 1.7 x 10(4) sequences obtained from repeated panning of the library.

85 Affinity panning of these resulted in the selection of variants w

86 patients to receive phage library for serial panning of tumor targeting ligands.

87 ingle-chain Fv antibody fragments (ScFvs) by panning on a prothrombin-coated surface.

88 By panning on B chain, we identified binders and have chara

89 Panning on carbonic anhydrase yielded a potent ligand, s

90 antibodies directly from phage libraries by panning on cells.

91 ffinities for digoxin increased 2-4-fold, by panning on digoxin-BSA yet retaining the specificity shi

92 approach involving preclearing steps before panning on HUVECs, we isolated and sequenced 140 individ

93 After three rounds of panning on recombinant mesothelin, a single-chain Fv (sc

94 on of profiles the phage library enriched by panning on the pool of cancer sera.

95 -specific T cells from animals with SGVHD by panning or by flow cytometric sorting.

96 odologies that rely on microdissection, cell panning or cell sorting, the TRAP methodology bypasses t

97 Panning peptide phage display libraries led to the ident

98 A third round of panning performed in the presence of both the fibrinogen

99 trate, S-adenosylmethionine (AdoMet), during panning permitted PIMT to retain aged phage in greater n

100 The CERVIGTGWVRC sequence was identified by panning phage display libraries on the inhibitory CD47 m

101 tended to specific application of M13qPCR in panning phage libraries on tissue sections of prostate a

102 e sera were then enriched using a multi-step panning procedure.

103 riments performed on this library during the panning process, this scFv bound 1200-fold less tightly

104 ealed by blockade of this epitope during the panning process.

105 es a number of aspects of conventional phage-panning protocols.

106 sequences that reacted specifically to their panning rAb by enzyme-linked immunosorbent assay.

107 we have demonstrated that a synthetic G(M3) panning reagent can be used to isolate a fully human scF

108 The initial panning recovered phage that bore the consensus motif Gl

109 However, panning rounds are followed by the tedious re-screening

110 body binding phages was observed after three panning rounds, and sequence analysis of randomly select

111 ycoproteins (Envs) dubbed sequential antigen panning (SAP).

112 on of binders to conserved Env structures by panning sequentially against Envs from different isolate

113 Six phage clones identified from S-20-4 panning showed significant binding to both S-20-4 and A-

114 to identify drivers of vascular invasion by panning small hairpin RNA (shRNA) library-transduced non

115 hlight the importance of exploring different panning strategies to optimize the selection of antihapt

116 om a panel of 11 Fabs recovered by different panning strategies, three highly potent neutralizing ant

117 tion using competitive panning and two novel panning strategies.

118 e positive clones and by using a subtractive panning strategy to remove crossreactivity with B. liche

119 rized nonimmune human Ab-phage library and a panning strategy with proteoliposomes and cells to ident

120 play antibody library by using an exhaustive-panning strategy.

121 en, and failure of clonal enrichment by cell panning suggested that the neoantigen was not membrane e

122 of the scFv fragments that were isolated by panning synthetic Ab libraries with different melanoma c

123 the variable region (scFvs) were isolated by panning synthetic scFv library 1 on purified HMW-MAA.

124 This was the first report of a modified panning technique to isolate adult pig RGCs.

125 ated from other retinal tissue by a modified panning technique using Thy 1.1 antibody.

126 The antigens used for panning the antibodies comprised two soluble, disulfide-

127 cFv library of the melanoma patient and then panning the libraries against melanoma cells to enrich f

128 ce homologies between peptides obtained from panning the library against the antibodies and the nativ

129 ice, measuring Ab responses to immunization, panning the phage library, high-throughput screening of

130 fragment of the V region (scFv) fragments by panning the synthetic phage Ab library (#1) with the hum

131 n any detectable MV Ags; after six rounds of panning, the positive clones comprised 34% of all phage

132 Panning this library against PAb1620 resulted in three u

133 in Java that supports real-time zooming and panning through a genome; layout of genomic features and

134 bs, a representative Fab was included during panning to block this common epitope on the F Ag.

135 We screened a phage library in vivo by panning to identify peptides that bound the vascular end

136 n of VHHs recognizing BDE-47 was achieved by panning under carefully modified conditions.

137 stable C(H)3 heterodimers were recovered by panning using an anti-flag antibody.

138 A second round of panning was performed against the same target mixture in

139 ner(s) for sperm acrosomal ligands, affinity panning was performed with mouse oocyte lysates using sp

140 In three patients, repeat panning was possible using phage recovered and amplified

141 rified by counterflow elutriation and lectin panning, was validated by real-time quantitative reverse

142 Using phage panning we have also identified several short peptide se

143 The tool offers smooth zooming and panning which is more flexible than seen in other browse

144 d to search for tumor-associated antigens by panning with a lung adenocarcinoma cell line, 1264, and

145 Yeast panning with a nonimmune human single-chain antibody lib

146 Panning with an allogeneic breast cancer cell line enric

147 population in the spleens enriched either by panning with anti-guinea pig immunoglobulin G-coated pla

148 However, panning with autologous tumor tissue lysate increased bi

149 By panning with DNA, the SLE libraries each yielded IgG ant

150 Selection of phage by panning with fixed neutrophils yielded a 195-fold enrich

151 from 7- to 15mer phage display libraries by panning with hyaluronan-Sepharose beads and assayed for

152 elected from randomly generated libraries by panning with recombinant SCR1-2, followed by specific li

153 ated from phage display peptide libraries by panning with self-tumor-associated Ag (TAA)-specific mAb

154 r EGFRvIII was isolated from this library by panning with successively decreasing amounts of syntheti

155 quid cultures 24 hours after HIV exposure by panning with the ICH3 CD34 MoAb (ICH3/CD34+) and maintai

156 In the last round (round two) of panning with this library, none of the phage clones that

157 nsitions, instead offering smoothly animated panning, zooming, navigation, and track selection.