ライフサイエンスコーパス: paraoxon

1 rocatalytic activity toward the reduction of paraoxon.

2 laucoma drug echothiophate and the pesticide paraoxon.

3 ampal kainate and systemic administration of paraoxon.

4 osarin, VX, and the OP pesticide metabolite, paraoxon.

5 agnitude lower than with its best substrate, paraoxon.

6 the kinetic constants for the hydrolysis of paraoxon.

7 nsecticides such as the parathion metabolite paraoxon.

8 osphate pesticides and nerve agents, such as paraoxon.

9 ter inhibition by the organophosphorus agent paraoxon.

10 ve to inhibition by organophosphates such as paraoxon.

11 -nitrophenyl acetate, and the organophospate paraoxon.

12 bonds at rates that rival the hydrolysis of paraoxon.

13 tants such as 1,2-dichloroethane (72 years), paraoxon (13 months), atrazine (5 months), and aziridine

14 ferred phosphotriester (P-O bond) substrate, paraoxon (5-100% kcat).

15 the identical experiment was performed with paraoxon, a "penetrating" AChE inhibitor, high K+ still

16 Both free ghrelin and paraoxon, a substrate for paraoxonase, can inhibit this

17 The concentration of paraoxon, an acetylcholine esterase inhibitor, can be qu

18 of achiral, chiral, and racemic mixtures of paraoxon analogues containing various combinations of me

19 Individual enantiomers of paraoxon analogues were also synthesized and the stereoc

20 The kinetic constants for these paraoxon analogues with the enzyme varied significantly

21 limit was found to be ~0.5 for OP pesticide paraoxon and 1 muM for non-OP pesticide carbaryl, in a w

22 rotect animals from exposure to a pesticide, paraoxon and a warfare agent, VX.

23 Esterase activity was inhibited by paraoxon and dichlorvos.

24 The lower detection limits were 3 microM for paraoxon and parathion and 5 microM for coumaphos.

25 domonas diminuta catalyzes the hydrolysis of paraoxon and related acetylcholinesterase inhibitors wit

26 Two known substrates are considered, paraoxon and sarin, although their turnover rates vary a

27 ates such as the widely utilized insecticide paraoxon and the chemical warfare agent sarin.

28 ydrolyze VR but has improved activity toward paraoxon and VX.

29 d excess of diethyl 4-nitrophenyl phosphate (paraoxon) and subsequent dealkylation, the broad 16.1 pp

30 d against methyl-parathion, malathion, ethyl-paraoxon, and methyl-paraoxon, respectively.

31 conjugates formed by sarin, cyclosarin, VX, paraoxon, and tabun are enhanced severalfold in vitro.

32 everal variants to hydrolyze phenyl acetate, paraoxon, and V-type OP nerve agents were examined.

33 ghly sensitive electrochemical biosensor for paraoxon as a model of organophosphates.

34 Using spectrofluorimetry and paraoxon as a model organophosphate, paraoxon concentrat

35 ecognition of OP-AChE that was prepared with paraoxon as an OP model agent.

36 araoxonase 1 (PON1) activity determined with paraoxon as substrate has been found to be associated wi

37 ature, and weight of cells immobilized using paraoxon as substrate.

38 irreversibly inhibited by echothiophate and paraoxon, but G117H regained 100% activity within 2-3 mi

39 substitution enhances ySFGH reactivity with paraoxon by >1000-fold ( k i (W197I) = 16 +/- 2 mM (-1)

40 c activity of Dr-OPH toward ethyl and methyl paraoxon by 126- and 322-fold and raised the specificity

41 the reaction mechanism of the hydrolysis of paraoxon by phosphotriesterase (PTE).

42 000-fold for hydrolysis of echothiophate and paraoxon by the G117H mutant compared to the nonenzymati

43 Paraoxon, chosen as nerve agent simulant, is linearly de

44 The reduction peak current vs paraoxon concentration was linear over the range 50nM to

45 try and paraoxon as a model organophosphate, paraoxon concentrations as low as 8 x 10(-7) M could be

46 inear relationship with the logarithm of the paraoxon concentrations in the range of 0.05-50mugL(-1)

47 organophosphate (sarin, cyclosarin, VX, and paraoxon) conjugates of human acetylcholinesterase (hACh

48 d onto a quartz substrate for utilization in paraoxon detection.

49 d for all of the mutants with the substrates paraoxon, diethylphenylphosphate, acephate, and diisopro

50 exposure to lethal doses of soman, sarin, or paraoxon effectively and safely counteracted their toxic

51 Ps used were chlorpyriphos-oxon (CPO), ethyl paraoxon (EPOx) and malaoxon (MOx).

52 s employed for the amperometric detection of paraoxon-ethyl, fenitrothion and chlorpyrifos ranging fr

53 ere were significant increases in the Km for paraoxon for all mutants except F132H.

54 hos>methylparathion>parathion>methylparaoxon>paraoxon>fench lorphos>profenofos>malathion.

55 and a decrease in the kinetic constants for paraoxon (I).

56 anato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10(-9) to 10(

57 easuring the hydrolysis of phenylacetate and paraoxon in serum samples of 87 patients with type 2 DM

58 roduction from hydrolysis of an insecticide, paraoxon, in a coupled assay involving phosphotriesteras

59 properties of the developed electrode toward paraoxon indicated that the nanocomposite possesses a pr

60 drolysis and oxidation of acetylcholine, and paraoxon-induced inhibition of acetylcholinesterase acti

61 ther administered during (kainate) or after (paraoxon) induction of SE.

62 m oximes to reactivate VX-, tabun- and ethyl paraoxon-inhibited human AChE.

63 The structure of the paraoxon-inhibited W197I variant was determined by molec

64 In comparison, the insecticide paraoxon irreversibly inhibited mosquito and human AChEs

65 The P=O bond of the substrate paraoxon is activated by adopting a tight coordination t

66 Low serum PON1 activity toward paraoxon is an independent risk factor for coronary even

67 VR inhibits H115W HuPON1 competitively when paraoxon is the substrate and noncompetitively when VX i

68 ncement of the rates of phosphorylation with paraoxon ( k i = 42 or 80 mM (-1) h (-1), respectively)

69 Km of (2.0 +/- 1.3) x 10(-1) M(-1)s(-1)) and paraoxon (kcat/Km of (4.6 +/- 0.8) x 10(3) M(-1)s(-1)),

70 or was used to measure as low as 2 microM of paraoxon, methyl parathion, and diazinon.

71 2000 V, baseline resolution is observed for paraoxon, methyl parathion, fenitrothion, and ethyl para

72 osure but does not protect against parathion/paraoxon or nerve agents.

73 optimized conditions, the biosensor measured paraoxon, parathion, and coumaphos pesticides with high

74 his set included analogues of the pesticides paraoxon, parathion, and dimefox, and the nerve agents D

75 ble microplate format with three substrates: paraoxon, phenyl acetate and the lactone dihydrocoumarin

76 ociation between PON1 substrate specificity (paraoxon/phenylacetate substrate activity ratios) and -9

77 However, paraoxon prevented the K(+)-induced loss of [14C]ACh fro

78 The Arg192 (R192) PON1 isoform hydrolyses paraoxon rapidly, while the Gln192 (Q191) isoform hydrol

79 thion, malathion, ethyl-paraoxon, and methyl-paraoxon, respectively.

80 e of hydrolyzing beta-lactam antibiotics and paraoxon, respectively.

81 Phosphorylation of the enzyme by paraoxon results in covalent modification of the active

82 The cholinesterase inhibitor methyl paraoxon significantly decreased AChE staining intensity

83 with IC50 = 10(-7) M for the organophosphate paraoxon, similar to mammalian cell culture-derived AChE

84 , while the Gln192 (Q191) isoform hydrolyses paraoxon slowly.

85 ity in addition to the ability to react with paraoxon solutions.

86 Here we report the structures of paraoxon, soman, and sarin complexes of group-VIII phosp

87 icate that low PON1 activity determined with paraoxon substrate is independently associated with SLE

88 ith findings in controls, PON1 activity with paraoxon substrate was reduced both in white lupus patie

89 ose proximity to the pro-S-ethoxy arm of the paraoxon substrate, was mutated to arginine, alanine, hi

90 ys catalytic constants for the hydrolysis of paraoxon that are essentially the same as those of the w

91 of the nerve agents soman and sarin, and of paraoxon, the active metabolite of the insecticide parat

92 he substrate and can range from 6000 s-1 for paraoxon to 0.03 s-1 for the slower substrates such as d

93 AChE (mAChE) were fractionally inhibited by paraoxon to form diethyl phosphoryl enzyme.

94 d inhibitor 4-nitrophenyl diethyl phosphate (paraoxon) to the free enzyme at pH 7.5, and subsequent d

95 ly hydrolyse 4-methylumbelliferyl acetate in paraoxon-treated cells, while the native enzyme was foun

96 ilar antiepileptogenic effect was found when paraoxon-treated rats were exposed to isoflurane after S

97 cally toward the pesticides ethyl and methyl paraoxon, using structure-based and random approaches.

98 es for the mutant enzymes with the substrate paraoxon varied from near wild type values to a 4-order

99 H on the cell surface degraded parathion and paraoxon very effectively without any diffusional limita

100 se reacted with the organophosphate compound paraoxon via its active site Ser273.

101 The detection limit for paraoxon was found to be 1.1 ppb and that for carbofuran

102 Serum PON1 activity toward paraoxon was measured in 1353 participants.

103 plete protection against the lethal doses of paraoxon was observed with nano-OPH administered iv and

104 Paraoxon was studied as an example of OPs.

105 Echothiophate and paraoxon were hydrolyzed with the same kcat of 0.75 min-

106 N1s are better able to promote hydrolysis of paraoxon, whereas HuPON1 is considerably better at catal

107 drolase (OPH), and to detect the presence of paraoxon, which is an organophosphorus compound, using t

108 osensor was used to measure as low as 0.4 pM paraoxon with a 6-min inhibition time.

109 F26G/C72I mutant catalyzed the hydrolysis of paraoxon with a kcat of 1.14 min-1, an increase of 16-fo

110 ound to catalyze the very slow hydrolysis of paraoxon with values of kcat and kcat/Km of 0.07 min-1 a

111 N male mice challenged with a lethal dose of paraoxon, with complete elimination of short-term clinic