ライフサイエンスコーパス: paraspeckle

1 stributed in nuclei and is also localized to paraspeckles.

2 action of non-structural RNA components with paraspeckles.

3 nucleus, where hLincRNA-p21 colocalizes with paraspeckles.

4 ed to be components of nuclear bodies called paraspeckles.

5 asm and retained in subnuclear bodies called paraspeckles.

6 n turn affecting the organization of nuclear paraspeckles.

7 d cells, and FUS deficiency leads to loss of paraspeckles.

8 involved in the assembly of Cajal bodies and paraspeckles.

9 edited, consistent with a structural role in paraspeckles.

10 ression results in the disruption of nuclear paraspeckles.

11 tial structural/organizational components of paraspeckles.

12 nuclear bodies (38), nuclear speckles (27), paraspeckles (24), Cajal bodies (17), Sam68 nuclear bodi

13 ue, Hennig et al. show that formation of the paraspeckle, a nuclear body that regulates gene expressi

14 previously been shown to localize to nuclear paraspeckles, a structure implicated in retaining unspli

15 addition, in the absence of Neat1-nucleated paraspeckles, a subset of Ctn RNA localizes to the perin

16 Furthermore, the formation of paraspeckles, after release from transcriptional inhibit

17 on/beta transcripts are localized to nuclear paraspeckles and directly interact with NONO.

18 NEAT1 in HeLa cells results both in loss of paraspeckles and in enhanced nucleocytoplasmic export of

19 While interaction of protein components of paraspeckles and Neat1 is understood, there is limited i

20 can be displaced by transfected PS-ASO from paraspeckles and rapidly degraded.

21 that is an essential structural component of paraspeckles and the hypoxic induction of NEAT1 induces

22 red for its colocalization with NEAT1 RNA in paraspeckles, and biochemical analyses support that NEAT

23 d PSP1 alpha are all expressed in hESCs, but paraspeckles are absent and only appear upon differentia

24 Nuclear paraspeckles are built co-transcriptionally around a lon

25 dies, nuclear speckles, Polycomb bodies, and paraspeckles are membraneless subnuclear organelles.

26 Paraspeckles are multifunction nuclear structures that s

27 Paraspeckles are nuclear bodies form around the long non

28 Paraspeckles are nuclear bodies formed by a set of speci

29 Paraspeckles are sub-nuclear domains that are nucleated

30 eat1, a noncoding RNA (ncRNA) constituent of paraspeckles, as a p53 target gene broadly induced by mo

31 trate that PABPN1 and MATR3 are required for paraspeckles, as well as for adenosine to inosine (A to

32 localizes with the RNA/DNA helicase Dhx9 and paraspeckles; as well as GW/P-bodies in the cytoplasm.

33 Paraspeckle assembly and function depend on expression o

34 in of FUS function can trigger disruption of paraspeckle assembly, which may impair protective respon

35 with adenosine-to-inosine editing and is in paraspeckle-associated complexes containing the proteins

36 Such nuclear retention correlates with paraspeckle-associated protein complexes containing p54(

37 s uncorrelated with nuclear localization and paraspeckle association.

38 ts, suggesting that oriPts interact with the paraspeckle-based innate antiviral immune pathway.

39 gether, our results indicate that functional paraspeckles can form with short nucleic acids other tha

40 Purification of the paraspeckle complex from adipocytes further showed that

41 NA-binding protein PSPC1, a component of the paraspeckle complex, promotes adipogenesis in vitro and

42 ear export of these mRNAs by methylating the paraspeckle component p54(nrb), which reduces the bindin

43 ts, and down-regulating synthesis of another paraspeckle component, the long noncoding RNA NEAT1, whi

44 neurodegenerative disorders, is an essential paraspeckle component.

45 nation microscopic analyses revealed that in paraspeckles, Ctn RNA partially co-localized with Neat1,

46 Collectively, these results show that while paraspeckles do not influence nuclear retention of Ctn R

47 otein:protein interaction involving the NONA/paraspeckle domain, which is characteristic of the DBHS

48 expression increases paraspeckle number, and paraspeckles emanate exclusively from the NEAT1 transcri

49 s and the hypoxic induction of NEAT1 induces paraspeckle formation in a manner that is dependent upon

50 FUS also regulates NEAT1 levels and paraspeckle formation in cultured cells, and FUS deficie

51 ing Neat1 expression in mice, which prevents paraspeckle formation, sensitized preneoplastic cells to

52 the long noncoding RNA NEAT1, which inhibits paraspeckle formation.

53 ress NEAT1 transcription, leading to reduced paraspeckle formation.

54 on FUS mutations might be expected to affect paraspeckle function in human diseases because mislocali

55 together with PABPN1 is required for normal paraspeckle function.

56 lication stress, stimulated the formation of paraspeckles in mouse and human cells.

57 nd further substantiates a critical role for paraspeckles in the mechanism of action of Rev.

58 cts with p54nrb/NONO, a major constituent of paraspeckles, in an RNA-dependent manner and responds in

59 b was observed in canonical NEAT1-containing paraspeckles, in perinucleolar caps upon transcriptional

60 that, in pituitary cells, all components of paraspeckles including four major proteins and Neat1 dis

61 aspeckle number and size, we investigate how paraspeckles influence the nuclear organization of their

62 NEAT1 RNA, a long noncoding RNA required for paraspeckle integrity, abolished the ability of overexpr

63 dicating the dependence of RBM14 function on paraspeckle integrity.

64 The induced formation of paraspeckle-like and filament structures occurred in mou

65 s can serve as seeding molecules to assemble paraspeckle-like foci in the absence of NEAT1 RNA.

66 ization of Ctn RNA, where it formed enlarged paraspeckle-like foci.

67 nscriptional inhibition, and importantly, in paraspeckle-like or filament structures lacking NEAT1 RN

68 o paraspeckle-like structures, implying that paraspeckle-like structures assembled on PS-ASOs are fun

69 hologically normal and apparently functional paraspeckle-like structures containing no NEAT1 RNA.

70 raspeckles, was also observed to localize to paraspeckle-like structures, implying that paraspeckle-l

71 t1, and displayed a more heterogeneous intra-paraspeckle localization.

72 reactivation resulted in increased number of paraspeckle-localized Ctn RNA foci.

73 nuclear structures and co-localizes with the paraspeckle marker p54NRB/NONO, suggesting a role in tra

74 These results suggest that paraspeckles may potentially form through self-associati

75 mmon lncRNA target with downstream impact on paraspeckle morphology and function.

76 mouse model of OPMD and demonstrate altered paraspeckle morphology in the presence of endogenous lev

77 1 is an essential architectural component of paraspeckle nuclear bodies, whose pathophysiological rel

78 that we show are completely coincident with paraspeckles, nuclear domains implicated in mRNA nuclear

79 Here, by varying paraspeckle number and size, we investigate how paraspec

80 sion of PSP1, NEAT1 overexpression increases paraspeckle number, and paraspeckles emanate exclusively

81 establishes a key genetic link between NEAT1 paraspeckles, p53 biology and tumorigenesis and identifi

82 of Neat1, Ctn RNA continues to interact with paraspeckle protein NonO to form residual nuclear foci.

83 Further, the multifunctional paraspeckle protein, NONO, was found to bind to oriPt tr

84 is (ALS)-linked FUS variants sequester other paraspeckle proteins into aggregates formed in cultured

85 esting that it controls sequestration of the paraspeckle proteins PSP1 and p54, factors linked to A-I

86 manner and responds in the same way as other paraspeckle proteins to alterations in cellular homeosta

87 ntisense oligonucleotides (ASOs) can recruit paraspeckle proteins to form morphologically normal and

88 PS-ASOs can associate with paraspeckle proteins, including P54nrb, PSF, PSPC1 and h

89 -complexity prion-like domains (PLDs) within paraspeckle proteins.

90 l analyses support that NEAT1 RNA binds with paraspeckle proteins.

91 ns as an essential structural determinant of paraspeckles, providing a precedent for a ncRNA as the f

92 demonstrated that RBM14, a protein found in paraspeckle structures in the nucleus, is involved in HI

93 l emanating from either end of the dimer for paraspeckle subnuclear body formation.

94 oncoding RNA NEAT1 (Menepsilon/beta) to form paraspeckles, subnuclear bodies that alter gene expressi

95 Depletion of NEAT1 RNA via RNAi eradicates paraspeckles, suggesting that it controls sequestration

96 ns nuclear-retained in the absence of intact paraspeckles, suggesting that they do not regulate nucle

97 This study shows that paraspeckles, thanks to their circadian expression, cont

98 Concomitant with increase in number of paraspeckles, transcriptional reactivation resulted in i

99 export of transcripts containing IRAlus from paraspeckles under certain cellular stresses, such as po

100 RNA reported to be functionally retained in paraspeckles, was also observed to localize to paraspeck

101 etention of the egfp mRNA that was lost when paraspeckles were disrupted whereas insertion of a singl