ライフサイエンスコーパス: pastoris

1 meleagris recombinantly expressed in Pichia pastoris .

2 ecretion are necessary for sporulation in P. pastoris.

3 meric proteins in mammalian cells and Pichia pastoris.

4 ant-negative mutant forms of Sar1p in Pichia pastoris.

5 In this work, nicaTf was expressed in Pichia pastoris.

6 heterologously produced in the yeast Pichia pastoris.

7 man IgG1-Fc was first overproduced in Pichia pastoris.

8 f human ABC transporters in the yeast Pichia pastoris.

9 membrane insertion in both E.coli and Pichia pastoris.

10 as now been successfully expressed in Pichia pastoris.

11 gy of methanol-induced peroxisomes in Pichia pastoris.

12 protein expressed in and secreted by Pichia pastoris.

13 n of recombinant proteins produced by Pichia pastoris.

14 on pathway of the methylotropic yeast Pichia pastoris.

15 in 5 alone (Dom5His) was expressed in Pichia pastoris.

16 a recombinant proprotein in the yeast Pichia pastoris.

17 screte tER sites in the budding yeast Pichia pastoris.

18 Gase) were produced heterologously in Pichia pastoris.

19 CR, and the mutants were expressed in Pichia pastoris.

20 pressed recombinant F2 in E. coli and Pichia pastoris.

21 nt protein expression in both E. coli and P. pastoris.

22 cytoplasmic domains, was expressed in Pichia pastoris.

23 ombinant human clusterin in the yeast Pichia pastoris.

24 ecombinant Epo (R103A) from the yeast Pichia pastoris.

25 8 (SFA8) in the methylotrophic yeast Pichia pastoris.

26 and PH4 proteins after expression in Pichia pastoris.

27 le, secretory protein using the yeast Pichia pastoris.

28 tors designed to express foreign genes in P. pastoris.

29 nd URA3-from the methylotrophic yeast Pichia pastoris.

30 inal after heterologous expression in Pichia pastoris.

31 orm of AChE C was highly expressed by Pichia pastoris.

32 ) mutants of the methylotrophic yeast Pichia pastoris.

33 e methylotrophic yeast Komagataella (Pichia) pastoris.

34 ed single rings in cells of the yeast Pichia pastoris.

35 gulator of multiple metabolic pathways in P. pastoris.

36 gulator of multiple metabolic pathways in P. pastoris.

37 main of CA IX in methylotrophic yeast Pichia pastoris.

38 th Atg30 regulates pexophagy in the yeast P. pastoris.

39 of human growth hormone secreted from Pichia pastoris.

40 s thaliana in the host organism Komagataella pastoris.

41 oulardii, Saccharomyces paradoxus, or Pichia pastoris.

42 t data from the allopolyploid Capsella bursa-pastoris.

43 We tested these ideas using the yeast Pichia pastoris.

44 in 293T cells, Escherichia coli, and Pichia pastoris.

45 btilis xylanase A (XynA) expressed in Pichia pastoris.

46 e methanol metabolism antagonistically in P. pastoris.

47 y recombinant expression in the yeast Pichia pastoris.

48 sion of Mxr1-activated genes by 14-3-3 in P. pastoris.

49 in expression in Escherichia coli and Pichia pastoris.

50 The abf3 gene was thus expressed in Pichia pastoris.

51 l for the stability and function of MS in P. pastoris.

52 is genes in the methylotrophic yeast, Pichia pastoris.

53 lected for heterologous expression in Pichia pastoris.

54 LL1 could be recovered upon expression in P. pastoris.

55 non-covalent dimer from secretion in Pichia pastoris (115 mg/l) and was a potent inhibitor of alpha

56 structure similar to that of Capsella bursa-pastoris, a distant mustard relative of Arabidopsis, sug

57 Mutant enzymes were expressed in Pichia pastoris, a methylotrophic yeast strain, and their kinet

58 .6.1) were cytosolically expressed in Pichia pastoris, a methylotrophic yeast, using spinach (Spinaci

59 whole-genome resequencing in Capsella bursa-pastoris, a recently formed tetraploid with one of the m

60 Capsella bursa-pastoris, a widespread ruderal plant, is a recent allote

61 When Pichia pastoris adapts from methanol to glucose growth, peroxis

62 Expression of an intronless ckx1 in Pichia pastoris allowed production of large amounts of recombin

63 Recombinant Pichia pastoris AMA1-FVO (PpAMA1-FVO) and PpAMA1-3D7 are O-link

64 globiformis amine oxidase (AGAO), and Pichia pastoris amine oxidase (PPLO) have been examined.

65 ch into recombinant protein production by P. pastoris and a synthetic biology approach to this indust

66 gD2 were produced in glycoengineered Pichia pastoris and administered intramuscularly as a bivalent

67 The proteins were expressed in Pichia pastoris and adsorbed on Alhydrogel.

68 lycosylated protease domain produced from P. pastoris and at 1.40 A for the mutagenically deglycosyla

69 mains were expressed in high yield in Pichia pastoris and baculovirus, respectively.

70 his report, we described two systems (Pichia pastoris and baculovirus/Sf9 cells) for expression of th

71 que feature of respiratory yeasts such as P. pastoris and C. albicans, and it may have novel moonligh

72 hat MS is localized to the nucleus of Pichia pastoris and Candida albicans but is cytoplasmic in Sacc

73 d O-linked mannosylation in the yeast Pichia pastoris and compared them to their unglycosylated count

74 Kv1.2 which has been overexpressed in Pichia pastoris and crystallised.

75 DF-2, are conserved between the yeast Pichia pastoris and D. discoideum.

76 DBL5 recombinant proteins produced in Pichia pastoris and developed a panel of seven chondroitin sulf

77 itro cell-free ER-budding assay using Pichia pastoris and followed two endogenous PMPs, Pex11p and Pe

78 enzyme was recombinantly produced in Pichia pastoris and homogeneously purified, and its glucose-oxi

79 the AvrLm4-7 protein was produced in Pichia pastoris and its crystal structure was determined.

80 GSA12 in Pichia pastoris and its Saccharomyces cerevisiae counterpart, C

81 perties of VKORC1L1 when expressed in Pichia pastoris and more particularly its susceptibility to vit

82 e former is additionally conserved in Pichia pastoris and Paracoccus denitrificans, suggesting that t

83 E proteins were also overexpressed in Pichia pastoris and purified for analysis.

84 ess P-glycoproteins were expressed in Pichia pastoris and purified in high yield in detergent-soluble

85 aquaporin in the methylotrophic yeast Pichia pastoris and purified the hexahistidine-tagged protein b

86 an ABCG5 and ABCG8 genes in the yeast Pichia pastoris and purified the proteins to near homogeneity.

87 GLU44 -encoded hydrolase was expressed in P. pastoris and purified to homogeneity.

88 an MATN-1 was cloned and expressed in Pichia pastoris and purified to homogeneity.

89 ple Bla g 2 mutants were expressed in Pichia pastoris and purified.

90 (sLRPs) can be produced in high yield in P. pastoris and readily purified.

91 se expressed in amylolytic strains of Pichia pastoris and Saccharomyces cerevisiae.

92 sed the human cytosolic ISCS in yeast Pichia pastoris and showed that the cytosolic form of ISCS is a

93 xpressed in both Escherichia coli and Pichia pastoris and shown to be active.

94 dopsis GT31 members were expressed in Pichia pastoris and tested for enzyme activity.

95 in glycoengineered lines of the yeast Pichia pastoris and that antibody-mediated effector functions c

96 e required for pexophagy and autophagy in P. pastoris and the Cvt pathway, autophagy, and pexophagy i

97 WCI5 and WCI2 were expressed in Pichia pastoris and the recombinant proteins were assayed again

98 the candidate genes were expressed in Pichia pastoris and their activities measured with the biochemi

99 ecombinant thaumatin was expressed in Pichia pastoris and through a co-expression strategy with a mol

100 re, we expressed recombinant hSMVT in Pichia pastoris and used affinity chromatography to purify the

101 CUA) pairs were shown to be orthogonal in P. pastoris and used to incorporate eight unnatural amino a

102 ls and the methylotropic yeast strain Pichia pastoris and was shown capable of penetrating into COS-7

103 tides were expressed recombinantly in Pichia pastoris and were tested for their ability to bind to hu

104 expressed in Escherichia coli and in Pichia pastoris, and analyzed for monoclonal antibody and IgE a

105 n 11, expressed these mutant forms in Pichia pastoris, and determined binding kinetics with human IGF

106 n cysteine protease, was expressed in Pichia pastoris, and its physicokinetic properties were determi

107 ichia coli, Saccharomyces cerevisiae, Pichia pastoris, and mammalian cell lines.

108 fragment was efficiently (15)N-labeled in P. pastoris, and proton cross-peaks were well dispersed in

109 Trp, the mutant proteins expressed in Pichia pastoris, and purified to homogeneity.

110 peptide was recombinantly produced in Pichia pastoris, and the three-dimensional structure was solved

111 eglycosylated form, both expressed in Pichia pastoris, are investigated and compared as biocatalysts

112 y RACE-PCR and expressed in the yeast Pichia pastoris as a secreted enzyme.

113 ults demonstrate the utility of using Pichia pastoris as an efficient eukaryotic host to express larg

114 expressed in the methylotrophic yeast Pichia pastoris at a secretion yield of approximately 10 mg x L

115 ms of AMA1 that were both produced in Pichia pastoris at a sufficient economy of scale to be usable f

116 d by autophagy receptors, such as the Pichia pastoris autophagy-related protein 30 (Atg30), which con

117 When expressed in Pichia pastoris, AxlA had activity comparable to the native enz

118 8.0 was purified from the engineered Pichia pastoris broth to homogeneity by anion exchange chromato

119 cted in most pex mutants of the yeast Pichia pastoris but is severely reduced in pex4 and pex22 mutan

120 n pathway in the methylotrophic yeast Pichia pastoris by binding to Mxr1p response elements (MXREs) p

121 rm of P. falciparum AMA1, produced in Pichia pastoris, by vaccinating Aotus vociferans monkeys and th

122 cerevisiae, the methylotrophic yeast Pichia pastoris can assimilate amino acids as the sole source o

123 staphylokinase (SakSTAR) expressed in Pichia pastoris cells have been determined.

124 L72Y], was generated and expressed in Pichia pastoris cells in yields exceeding 100 mg/liter.

125 uncharacterized 14-3-3 family protein in P. pastoris complements Saccharomyces cerevisiae 14-3-3 fun

126 TaGT47-13, TaGT75-3, and TaGT75-4 in Pichia pastoris confirmed that these proteins form a complex.

127 The budding yeast Pichia pastoris contains discrete tER sites and is, therefore,

128 The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete

129 ified recombinant PHACS, expressed in Pichia pastoris, contains bound pyridoxal-5'-phosphate (PLP), b

130 f expression of the RK gene in yeast (Pichia pastoris), COS-1 cells and in an HEK293 stable cell line

131 ction.We have identified Cvt9 and its Pichia pastoris counterpart Gsa9.

132 ect on AOXI gene expression and growth of P. pastoris cultured in a minimal medium containing yeast n

133 acts as a negative regulator of PEPCK in P. pastoris cultured in biotin-deficient, glucose-ammonium

134 Deletion of Mxr1 retards the growth of P. pastoris cultured in YNBA supplemented with casamino aci

135 ression of AOXI and growth retardation of P. pastoris cultured in YPM medium.

136 ase of recombinant AMA1, the E. coli- and P. pastoris-derived antigens are immunologically and functi

137 of the natural allergen, whereas the Pichia pastoris-derived glycosylation does not.

138 tors that are composed almost entirely of P. pastoris DNA (except for the recombinant gene) and are d

139 on processes where strains containing non-P. pastoris DNA sequences, particularly bacterial antibioti

140 demonstrated on a flux experiment of Pichia pastoris employing two different tracers, i.e., 1,6(13)C

141 ts suggest that polyploidization in C. bursa-pastoris enhanced its plasticity of response to light an

142 The recombinant enzyme expressed in Pichia pastoris established a 1.3:1 equilibrium between UDP-D-g

143 btained by heterologous expression in Pichia pastoris exhibited greater XET activity against xylogluc

144 All three Na-GSTs, when expressed in Pichia pastoris, exhibited low lipid peroxidase and glutathione

145 mlined workflow for the generation of Pichia pastoris expression strains, reducing the timeline by a

146 ch domain of PyP140/RON4 by using the Pichia pastoris expression system and characterized the recombi

147 or domain 9 alone were expressed in a Pichia pastoris expression system and tested for their ability

148 The Pichia pastoris expression system offers economy, ease of manip

149 human MD-2 (rhMD-2) was produced in a Pichia pastoris expression system, and the interaction between

150 Using the Pichia pastoris expression system, we show that cleavage of inh

151 ring of the DNA encoding VCP into the Pichia pastoris expression system, were used to localize the re

152 by small-scale fermentation using the Pichia pastoris expression system.

153 produced using Escherichia coli and a Pichia pastoris expression system.

154 er its heterologous expression in the Pichia pastoris expression system.

155 ide the FLD1 selection system in a set of P. pastoris expression vectors that are composed almost ent

156 e chitinase, rChiA, was purified from Pichia pastoris extracellular medium by differential precipitat

157 tandardization by fully (13)C labeled Pichia pastoris extracts enabled absolute quantification of the

158 mplified using unclarified broth from Pichia pastoris fermentation as feedstock.

159 used as a marker in transformations of a P. pastoris fld1 host by selection on plates containing met

160 eins expressed in Escherichia coli or Pichia pastoris for their ability to induce immunity and protec

161 the use of a novel selectable marker, the P. pastoris formaldehyde dehydrogenase gene (FLD1) for DNA-

162 ort that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost normal gro

163 However, FBP1 when expressed in Pichia pastoris generated H2O2 using cysteine at pH 7.2, a spec

164 structed a high-quality assembly of C. bursa-pastoris genome and a transcriptome atlas covering a bro

165 elements and stably incorporated into the P. pastoris genome.

166 In P. pastoris, Golgi stacks are adjacent to discrete tER site

167 Coexpression of the two genes in P. pastoris greatly increased the yield of pure proteins, i

168 in (Pgp; mouse MDR3) was expressed in Pichia pastoris, grown in fermentor culture, and purified.

169 In P. pastoris, Gsa12 appears to be required for an early even

170 In P. pastoris Gsa9 is recruited to concentrated regions on th

171 In P. pastoris, Gsa9 is required for glucose-induced pexophagy

172 ion was obtained using methanol-inducible P. pastoris (> 95% pure protein, yield approximately 48 mg

173 Recombinant EhCP5 expressed in Pichia pastoris had kinetic properties similar to those of the

174 We propose that P. pastoris has discrete tER sites and therefore generates

175 P. pastoris has the capacity to produce large quantities of

176 The methylotrophic yeast, Pichia pastoris, has been genetically engineered to produce man

177 Because yeasts such as Pichia pastoris have been shown to O-glycosylate some proteins

178 e salt-stimulated lipase expressed in Pichia pastoris, hen ovalbumin, bovine fetuin, bovine thyroglob

179 Finally, we demonstrate that the Pichia pastoris homologue Gsa7p that is required for peroxisome

180 cal results demonstrate that Cvt9 and the P. pastoris homologue Gsa9 may function at the step of sele

181 We propose that MXR1 is the P. pastoris homologue of S. cerevisiae ADR1 but that it has

182 expressed in the methylotropic yeast Pichia pastoris indicate that it catalyzes the 4-epimerization

183 de ABC transporter TAPL, expressed in Pichia pastoris, into lipid vesicles (liposomes) and performed

184 Pichia pastoris is a methylotrophic yeast that has been genetic

185 The methylotrophic yeast Pichia pastoris is a popular host for the production of a varie

186 Pichia pastoris is a simple and powerful expression platform th

187 P. pastoris is an excellent system for producing the large

188 ethanol-inducible expression of AOXI when P. pastoris is cultured in a nutrient-rich medium containin

189 nol assimilation are synthesized when Pichia pastoris is grown in methanol.

190 When P. pastoris is grown on a mixed carbon source containing bo

191 1 protein heterologously expressed in Pichia pastoris is labeled by using all-trans [3H]retinal, sugg

192 1p, the ER-to-peroxisome translocation in P. pastoris is phosphorylation independent, and the phospho

193 The yeast Pichia pastoris is used extensively as the host cell for large-

194 gatus Cu,Zn SOD has been expressed in Pichia pastoris, is enzymatically active, and has biochemical a

195 r transporting aquaporin of the yeast Pichia pastoris, is suggested to be gated by chemo-mechanical s

196 promoter of the methylotrophic yeast, Pichia pastoris, is used widely for the production of recombina

197 were synthesized in the yeast species Pichia pastoris: K195M, K199M, F211V, W214L, R218M, R222M, H242

198 , Pisum sativum amine oxidase (PSAO), Pichia pastoris lysyl oxidase (PPLO), bovine plasma amine oxida

199 richia coli amine oxidase (ECAO), and Pichia pastoris lysyl oxidase (PPLO).

200 ncreatic alpha-amylase, concanavalin, Pichia pastoris lysyl oxidase, and Klebsiella pneumoniae acetol

201 gnaporthe oryzae(Mo), was expressed inPichia pastoris.Mo-MnLOX was deglycosylated, purified to homoge

202 Nuclear localization of P. pastoris MS (PpMS) was abrogated by the deletion of 107

203 We isolated a Pichia pastoris mutant that was unable to grow on the peroxisom

204 In oleate medium, the P. pastoris mutants pex11A (constitutively unphosphorylated

205 this gene heterologously in the yeast Pichia pastoris, obtaining a relatively high yield of 2.2 mg of

206 D1 gene (Fld1p) is required for growth of P. pastoris on methanol as a carbon source and methylamine

207 Growth of the yeast Pichia pastoris on methanol induces the expression of genes who

208 lds cultivating the heterologous host Pichia pastoris on the 5L bioreactor scale (reUmChlE; 45.9UL(-1

209 The protein expressed in P. pastoris, on the other hand, was expressed as a secreted

210 nding-like (DBL) domains expressed in Pichia pastoris or var2csa plasmid DNA and sera were screened o

211 In this study, we show the P. pastoris ortholog of Atg9, a novel membrane protein is e

212 eraction between Pex19p and all known Pichia pastoris Pex proteins by the yeast two-hybrid assay.

213 As has been shown previously for P. pastoris pex1, pex6, and pex22 mutant cells, we show her

214 we examined the behavior of PMPs in a Pichia pastoris pex17 mutant.

215 Pichia pastoris PEX17 was cloned by complementation of a peroxi

216 t the cloning and characterization of Pichia pastoris PEX19 by complementation of a peroxisome-defici

217 in, Yaf5p, is the functional homologue of P. pastoris Pex22p.

218 We show that Pichia pastoris Pex8p (PpPex8p) enters the peroxisome matrix us

219 The Pichia pastoris pexophagy receptor Atg30 is recruited to peroxi

220 as tested with human LAL expressed in Pichia pastoris (phLAL) and CHO cells (chLAL), respectively.

221 ose terminated human LAL expressed in Pichia pastoris (phLAL), purified, and administered by tail vei

222 Pichia pastoris (Pp) Pex8p, the only known intraperoxisomal per

223 ced in both H. jecorina (HjCel3A) and Pichia pastoris (Pp-HjCel3A).

224 EcCSP) or in the methylotrophic yeast Pichia pastoris (PpCSP) for structural analyses.

225 e show that the ScPex11p homologue in Pichia pastoris (PpPex11p) is phosphorylated at serine 173.

226 rity of glycosylated enzymes expressed in P. pastoris presented increased thermostability in comparis

227 at both sites, whereas in Pp-HjCel3A, the P. pastoris-produced HjCel3A enzyme, the glycan chains cons

228 The P. pastoris-produced protein was superior to that produced

229 urthermore, we also demonstrated that Pichia pastoris produces XynCDBFV with higher catalytic activit

230 at neural agrin (AgG3z8) expressed in Pichia pastoris promoted AChR clustering on surface of C2C12 my

231 We show how a Pichia pastoris protein, PpAtg30, mediates peroxisome selection

232 Glycoengineered P. pastoris provides a general platform for producing recom

233 ts of bile acids on ATP hydrolysis in Pichia pastoris purified ABCG5/G8 and found that they stimulate

234 eta1, alpha2beta2, and alpha2beta3 in Pichia pastoris, purified the complexes, and compared their fun

235 fferent variants of MDR3 in the yeast Pichia pastoris, purified the proteins via tandem affinity chro

236 e show that the integrity of tER sites in P. pastoris requires the peripheral membrane protein Sec16.

237 tion in a cysteine protease, G79E, in Pichia pastoris resulted in an unstable precursor protein, cons

238 expression of this gene in the yeast Pichia pastoris resulted in the production of a beta-1,4 glucan

239 xpressed recombinantly in E. coli and Pichia pastoris, resulting in unglycosylated and mannosylated p

240 We find that P. pastoris Sec12 exchanges rapidly between tER sites and t

241 Redistribution of P. pastoris Sec12 to the general ER does not perturb the lo

242 P. pastoris Sec16 is an order of magnitude less abundant th

243 g alpha-1,6-mannosyltransferase gene from P. pastoris, several combinatorial genetic libraries were c

244 P3, and sLRP4) have been expressed in Pichia pastoris SMD1168 with constitutive coexpression of the r

245 um-5 produced in Escherichia coli and Pichia Pastoris specifically inhibited proliferation and caused

246 ced in the absence of SG, indicating that P. pastoris specifically uses sterol conversion by Atg26 to

247 Komagataella phaffii (formerly called Pichia pastoris) specifically, the indirect traffic of Pex2, bu

248 toplasmic in Saccharomyces cerevisiae The P. pastoris strain carrying a deletion of the MET6 gene enc

249 The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydr

250 dase (BfrA) secreted by a recombinant Pichia pastoris strain was optimally immobilised on Glyoxyl-Sep

251 platform using genetically engineered Pichia pastoris strains designed to secrete multiple proteins o

252 ted proteins and two different strains of P. pastoris , suggesting its general nature.

253 oleate- and amine-induced peroxisomes in P. pastoris, suggesting that the function of sterol glucosi

254 In the Pichia pastoris system, the protease domain was expressed as a

255 were overproduced recombinantly in a Pichia pastoris system, they displayed the dual inhibitory prop

256 The experimental approach was to analyze P. pastoris tER-Golgi units by using cryofixed and freeze-s

257 ulans) and in a methylotrophic yeast (Pichia pastoris), the latter expression system producing as muc

258 with a glycosylated form expressed in Pichia pastoris, the [(15)N,(1)H]-correlation spectra of both e

259 In Pichia pastoris, the orientation of a 138-kb invertible region

260 In Pichia pastoris, the peroxisomal targeting signal 2 (PTS2)-depe

261 Opinion piece, we use Pichia (Komagataella) pastoris to illustrate the limitations of the available

262 expressed in the methylotrophic yeast Pichia pastoris to obtain a post-translationally modified and f

263 expressed in the methylotrophic yeast Pichia pastoris to probe for the proposed phosphatidylcholine (

264 e established an expression system in Pichia pastoris to recombinantly produce and purify Cx43 as wel

265 he glycosylation pathway in the yeast Pichia pastoris to secrete a human glycoprotein with uniform co

266 its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully compl

267 Here, we have used P. pastoris to test various models for Golgi trafficking.

268 s to alter the N-glycosylation pathway in P. pastoris to yield N-linked oligosaccharides with hybrid

269 terodimers overexpressed in the yeast Pichia pastoris, together with docking analysis and crosslinkin

270 Here we successfully generated a Pichia pastoris transformant expressing and secreting apidaecin

271 ltransferase I (L-CPT I) expressed in Pichia pastoris, two contiguous discrete sequences within its N

272 he genome of the methylotrophic yeast Pichia pastoris under the control of an AOX promoter and the ye

273 ssed as a secreted soluble protein in Pichia pastoris under the regulation of alcohol oxidase 1 promo

274 is agglutinin; GNA) were expressed in Pichia pastoris using native signal peptides, or the Saccharomy

275 We also show that the P. pastoris Vac8 armadillo repeat protein is not essential

276 observed against both rh-Endo and the Pichia pastoris vector, but no allergic reactions were observed

277 The istk gene was expressed in Pichia pastoris vectors.

278 on system in the methylotrophic yeast Pichia pastoris was developed.

279 binant protein expressed in the yeast Pichia pastoris was found to have activity against the importan

280 The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequen

281 an Fv-p53 fusion protein produced in Pichia pastoris was tested on CT26.CL25 colon cancer cells in v

282 In-cell NMR in the yeast Pichia pastoris was used to study the influence of metabolic ch

283 recombinant cathepsin F, produced in Pichia pastoris, was processed to its active mature form autoca

284 is, we expressed human CFH mutants in Pichia pastoris We found that recombinant I62-CFH (protective a

285 ays of BdCSLF6 expressed in the yeast Pichia pastoris, we also demonstrate that the catalytic domain,

286 oleate- and amine-induced peroxisomes in P. pastoris were reduced in the absence of SG, indicating t

287 length enzyme (expressed in the yeast Pichia pastoris) were quantified.

288 idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharom

289 ACC synthase is now expressed in Pichia pastoris with an improved yield of 10 mg/L.

290 ing damage, we transformed the yeast, Pichia pastoris, with an inducible DEA1 construct.

291 verexpressing PfCRT to high levels in Pichia pastoris yeast by synthesizing a codon-optimized version

292 single Escherichia coli bacteria and Pichia pastoris yeast cell in the current study.

293 n vectors and transfected into E. coli or P. pastoris yeast cells.

294 so observed in another expression system, P. pastoris yeast cells.

295 um malarial parasite transporter PfCRT in P. pastoris yeast.

296 cessfully overexpressed PfMDR1 protein in P. pastoris yeast.

297 brane of Saccharomyces cerevisiae and Pichia pastoris yeast.

298 we expressed recombinant alpha2AP in Pichia pastoris yeast.

299 from Escherichia coli (bacteria) and Pichia pastoris (yeast) immobilized in a microfluidic chamber,

300 Chy1 was expressed in Pichia pastoris yielding an enzyme with a chymotrypsin specific