ライフサイエンスコーパス: pause

1 KOW5 specifically shift the location of the pause.

2 ity to promote microtubule polymerization or pause.

3 r alone, produce a substantial transcription pause.

4 polymerase II release from promoter-proximal pause.

5 ereas cargos driven by more motors tended to pause.

6 d higher MT coverage correlates with shorter pauses.

7 e between end-inspiratory and end-expiratory pauses.

8 ers, including synchronized burst firing and pauses.

9 ates of synthesis interspersed with distinct pauses.

10 loop induction correlates to transcriptional pausing.

11 EGR1 or MEF2C depending on RNA Polymerase II pausing.

12 cumulate at microtubule plus ends and induce pausing.

13 re regulated at the level of transcriptional pausing.

14 the regulation of RNA polymerase II (Pol II) pausing.

15 promoter-proximal RNA polymerase II (Pol II) pausing.

16 ts, with RelA activation requiring ribosomal pausing.

17 vivo evidence that MTERF1 stimulates TWINKLE pausing.

18 nscriptional initiation with transcriptional pausing.

19 protein structural motifs and translational pausing.

20 duction of unstable RNAs and transcriptional pausing.

21 hotspots does not appear to be stimulated by pausing.

22 EFb recruitment and promoter-proximal Pol II pausing.

23 g start times and coping with DNA polymerase pausing.

24 gation, is dispensable for promoter-proximal pausing.

25 ction neurons was sufficient to interrupt or pause a self-initiated bout of licking.

26 n SS activity, and then, following the CS, a pause, a rebound, and finally a late inhibition of SS ac

27 Despite the prevalence of Pol II pausing across the metazoan genomes, little is known abo

28 However, it is unclear how pausing affects the initiation of new transcripts during

29 imary miRNAs with significant RNA polymerase pausing alterations after JQ1 treatment; each miRNA was

30 vation of SK channels, leading to arrhythmic pauses alternating with bursts of pacing.

31 m the existence of a previously hypothesized paused and backtracked RNAP initiation intermediate and

32 rregular replication dynamics, with frequent pauses and direction reversals as the polymerase cycles

33 proteins are needed to explain the frequent pauses and occasional reversals observed in live-cell im

34 Lack of promoter-proximal pausing and a higher correlation of nascent and steady-s

35 c ablation of Cobra1, which encodes a Pol II-pausing and BRCA1-binding protein, ameliorates R-loop ac

36 ing domain of ZEB2 protein induces ribosomal pausing and compromises protein synthesis.

37 e translation process by increasing ribosome pausing and density on the mRNA.

38 RNA and influences transcription by inducing pausing and facilitating the process of transcription te

39 into the relationship between polymerase II pausing and gene regulation.

40 RNA polymerase II (Pol II) promoter-proximal pausing and its release.

41 n could be a major source of transcriptional pausing and lead to conflicts with other RNA polymerases

42 omoters have a strong disposition for Pol II pausing and often mediate faster, more synchronous chang

43 Frequent pausing and slipping caused by changes A78V and R79K sug

44 in that regulates RNA polymerase II (RNAPII) pausing and transcription elongation.

45 rapid transitions through phases of growth, pause, and catastrophe, continuously exploring and adapt

46 l to initiate timely MZT, undergo cell-cycle pause, and remain developmentally delayed throughout lar

47 anges, resulting in sinus bradycardia, sinus pauses, and a susceptibility to atrial arrhythmias, whic

48 d a lack of enhancer RNAs, promoter-proximal pausing, and divergent transcription in Arabidopsis seed

49 ssing KOW4 and KOW5 of Spt5 is essential for pausing, and mutations in KOW5 specifically shift the lo

50 portant roles in determining motor velocity, pausing, and processivity.

51 s detection of previously unknown mtDNA TIS, pausing, and transcription termination sites with unprec

52 Our work revealed a long pause ("initiation pause," approximately 20 s) after synthesis of a 6-mer R

53 ficant heart rate slowing and frequent sinus pauses are observed in iNICD mice when compared with con

54 pausing, while Pol II recruitment and Pol II pausing are not correlated among non-NL genes.

55 hrink or pause, but the factors that promote pausing are poorly understood.

56 th bind to RNA polymerase (RNAP), regulating pausing as well as intrinsic and Rho-dependent terminati

57 5 patients (ventricular tachycardia, n = 44; pause/asystole, n = 36; polymorphic ventricular tachycar

58 tely reduces the duration of a translocation pause at Chi.

59 A major ribosome pause at CTC leucine codons in the native gene of FVIII

60 rmines axonal transport progression: cargoes pause at polymer termini, suggesting that switching MT t

61 Q protein (82Q) can also engage RNAP that is paused at a promoter-distal position and thus contains a

62 ages RNAP during early elongation when it is paused at a specific site just downstream of the phage l

63 hesis from the promoter; this synthesis then pauses at a defined site several nucleotides downstream

64 ET-seq, we have previously shown that Pol II pauses at both ends of protein-coding genes but with dif

65 5PSeq can identify translational pauses at rare codons that are often masked when using a

66 e observations explain the observed ribosome pausing at AAA codons during translation and demonstrate

67 We observed increased polymerase pausing at both genes and enhancers, suggesting that pau

68 Spt4/5 suppressed RNAPII pausing at the major H3/H4-induced arrest point, resulti

69 s), tRNA(Glu), and tRNA(Gln) causes ribosome pausing at the respective codons in yeast.

70 t major interference in the establishment of pausing at their cognate promoters.

71 premature dissociation, but not preferential pausing, at the annealed sites.

72 malities including bradycardic events, sinus pauses, atrioventricular block, premature ventricular co

73 tracked state during initiation and that the paused-backtracked initiation intermediate was populated

74 how translation can prevent transcriptional pausing, backtracking, and termination.

75 at a major function of the STN is to broadly pause behavior and cognition when stop signals, conflict

76 The slowdown and the changes in pausing behavior within the nucleosomal region allow us

77 ndary-onset music-CPS for both groups during pauses between musical phrases.

78 r vocalizations per unit time and had longer pauses between vocalizations and that the entropy of the

79 naturally diapaused blastocysts in vivo and paused blastocysts ex vivo display pronounced reductions

80 sable for establishing or maintaining Pol II pausing but is critical for the release of paused Pol II

81 c polymers that in cells can grow, shrink or pause, but the factors that promote pausing are poorly u

82 eplicative helicase may continue during such pauses, but a self-governing mechanism, where helicase s

83 nimals showed feeding patterns of bursts and pauses, but their function is unknown.

84 elling support in favor of inteins acting as pause buttons to arrest protein function until needed; t

85 Promoter-proximal pausing by RNA polymerase II (Pol II) is a key rate-limi

86 ein complex that regulates promoter-proximal pausing by RNA polymerase II (Pol II).

87 y 20 s) after synthesis of a 6-mer RNA; such pauses can serve as regulatory checkpoints.

88 no comparable evidence that similarly brief pauses can substitute for negative prediction errors.

89 Pausing can be induced directly in cultured ES cells and

90 Bivalent (poised or paused) chromatin comprises activating and repressing hi

91 preinitiation complex (PIC) and the adjacent paused complex (PC).

92 ed by an intermediate excitation, where each pause corresponds to one interstimulus interval.

93 In pausing-deficient embryos, the transforming growth facto

94 p blocking the RNA exit channel, was a major pausing determinant.

95 ing bursts increase and the duration of long pauses diminishes in environments richer in bacteria.

96 RNA polymerase II (Pol II) pauses downstream of the transcription initiation site b

97 CDK9 activity decreases the pause duration but also increases the productive initiat

98 ts illustrate that the torque causes shorter pause durations and fewer collisions between polymerases

99 with cell motility, we show that spontaneous pauses during T cell motility in vitro and in vivo coinc

100 dividual replisomes display both looping and pausing during priming, reconciling divergent models for

101 tream of the stop codon because of ribosomal pausing during translation termination.

102 fest enhanced recognition of a promoter-like pause element positioned hundreds of nucleotides downstr

103 ed with the known RNA polymerase II (pol II) pausing/elongation factors SPT5 and TRIM28-KAP1-TIF1beta

104 We show that paused ES cells display a remarkable global suppression

105 red for a post-initiation, promoter-proximal pause essential for regulation of lambdoid phage late ge

106 amined the intrinsic conductances that shape pauses evoked by current injections and synaptic stimula

107 t of A-type potassium channels in prolonging pauses evoked by GABAergic inhibition.

108 moters previously bound by the transcription pausing factor M1BP, containing paused Pol II and enrich

109 ogether constitute an autonomous microtubule pausing factor.

110 actor P-TEFb and prevents phosphorylation of pausing factors.

111 during apnea, the mechanical ventilator was paused for up to 2 min during normal breathing.

112 Correlation analysis of pause forces demonstrated that individual Cin8 molecules

113 demonstrate that the duration of polymerase pausing generally limits the productive frequency of tra

114 Paused genes also have lower cell-to-cell expression var

115 t on gene expression levels, with moderately paused genes being expressed more highly than other paus

116 genes being expressed more highly than other paused genes.

117 Increased pausing has a non-linear effect on gene expression level

118 Unexpectedly, we identify a pause in early elongation, specific to S. pombe, that re

119 ent (IPSC) that resulted in only a transient pause in firing.

120 g exponential phase, and is accompanied by a pause in growth.

121 The pause in the atmospheric CO2 growth rate provides furthe

122 y elongation and causes RNA polymerase II to pause in the promoter-proximal region of genes.

123 vely raised in vivo to generate a sufficient pause in the zippering process for the regulators to set

124 ed the onset of spontaneous movements with a pause in their firing.

125 tion in north Melanesia and a nearly 2,000-y pause in West Polynesia.

126 ll division, stretch triggers cells that are paused in early G2 phase to activate calcium-dependent p

127 (NAc) while discrete aversive stimuli elicit pauses in dopamine neuron firing and reductions in NAc d

128 We show that pauses in dopamine neuron firing, evoked by either stimu

129 question of how intrinsic conductances shape pauses in dopamine neurons.

130 cell velocities by reducing the frequency of pauses in human T cells migrating through confined space

131 ostburst, and synaptically evoked inhibitory pauses in subpopulations of midbrain dopamine neurons.

132 subunit Spt4 and resembles promoter-proximal pausing in metazoans.

133 Stopping or pausing in response to threats, conflicting information,

134 decreasing the duration of RNA polymerase II pausing in the promoter-proximal region, but how this is

135 as a model to investigate the role of Pol II pausing in vertebrate organogenesis.

136 rway pressure drop during an end-inspiratory pause [in cm H2O]).

137 ion, and loss of H3K9ac increases the pol II pausing index on a subset of genes in HeLa cells.

138 s responsive to stimulus have slightly lower pausing index on average than non-responsive genes, and

139 n with microtubules, but rather enhances its pause-inducing activity by preventing KIF21B detachment

140 loped ChIP-nexus method, we find that Pol II pausing inhibits new initiation.

141 oses and may reflect a conserved state among paused, initiating eukaryotic RNA polymerase II enzymes.

142 Our work revealed a long pause ("initiation pause," approximately 20 s) after syn

143 suggesting that enhancers can influence the pause-initiation limit to regulate transcription.

144 of transcription initiation in human cells ('pause-initiation limit').

145 The paused intermediate population was further increased whe

146 This pause is a key component of metazoan gene expression reg

147 The pause is induced by sigma70 binding to a repeat of the p

148 Here, we reveal that this pause is linked to the cell cycle.

149 ce that the STN causally implements stops or pauses is lacking.

150 drive angiogenesis.Promoter proximal RNAPII pausing is a rate-limiting transcriptional mechanism.

151 Our studies show that Pol II pausing is an important contributor to BRCA1-associated

152 tin opening) and that GAF-facilitated Pol II pausing is critical for HS activation.

153 The mechanism of how DSIF establishes pausing is not known.

154 ep, but the mechanism that triggers ribosome pausing is not known.

155 While promoter-proximal pausing is not observed in budding yeast, inhibition of

156 Most notably, in mammals pausing is positively correlated with histone H2A.Z occu

157 However, it is still unknown how Pol II pausing is regulated by Cet1.

158 pecific enhancer state and RNA Polymerase II pausing, linking transcription regulatory potential and

159 Cells appear to pause locomotion when WRAMP structures disassemble and t

160 Disrupting Pol II pausing machinery causes a severe reduction of HSC speci

161 nscription mechanism instructing HSC fate by pausing-mediated differential regulation of key signalin

162 The pauses occur during the elongation step, but the mechani

163 esophageal) pressure during end-inspiratory pause of a tidal breath and tidal stress as the transpul

164 Since misincorporation leads to a strong pause of transcription due to backtracking, our findings

165 T, providing an explanation for the frequent pauses of short MTs and the immobility of longer MTs.

166 r and promoter of growth, induces reversible pausing of mouse blastocyst development and allows their

167 on factor binding, mediate promoter-proximal pausing of Pol II, and/or interact with Pol II to modula

168 ral mechanism for inducing promoter-proximal pausing of Pol II.

169 n metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4

170 hat presents an accessible rut site promotes pausing of RNA polymerase (RNAP) at a single Rho-depende

171 Cet1, impairs promoter-proximal accumulation/pausing of RNA polymerase II (Pol II) independently of i

172 The promoter-proximal pausing of RNA polymerase II (Pol II) plays a critical r

173 In metazoans, the pausing of RNA polymerase II at the promoter (paused Pol

174 nucleosome in particular, contribute to the pausing of RNA polymerase II.

175 of a complex, which regulates transcription pausing of RNA-polymerase II.

176 nal enhancers regulate the promoter-proximal pausing of RNAPII, a key rate-limiting step required for

177 inG is a nonmotile kinesin that promotes the pausing of SHR-associated endosomes.

178 his was due, at least in part, to reversible pausing of the cell cycle preventing S phase associated

179 rase II (pol II) to escape promoter proximal pausing on chromatin.

180 is known about the in vivo effect of Pol II pausing on vertebrate development.

181 ion) and the second having an intermittently pausing or "stuttering" TW (i.e., stuttering trap; ST re

182 clinicians must decide if they should stop, pause, or continue treatment.

183 Likewise, the transition into a burst/pause pattern results from combinations of intrinsic ion

184 esponded very inefficiently to the cmlA(crb) pause peptide.

185 s responded normally to the secM translation pausing peptide, but the uL4 mutant responded very ineff

186 parable water volumes, the effect of shorter pause periods on BSF performance should be investigated.

187 ranscriptional burst, suggesting that Pol II pausing plays a dominant role in gene regulation.

188 ranscription pausing factor M1BP, containing paused Pol II and enriched with promoter-proximal Polyco

189 ide strong support for the residence time of paused Pol II elongation complexes being much shorter th

190 We propose that paused Pol II helps prevent new initiation between trans

191 ene expression by controlling the release of paused Pol II in a PAF1-dependent manner.

192 x of Cdk9 and cyclin T1, promotes release of paused Pol II into elongation, but the precise mechanism

193 ted factor 1 (PAF1) modulates the release of paused Pol II into productive elongation.

194 transcription factors play in transitioning paused Pol II into productive Pol II is, however, little

195 I pausing but is critical for the release of paused Pol II into the gene body at a subset of highly a

196 Here, we show that the release of the paused Pol II is cooperatively regulated by multiple P-T

197 However, it remains largely unclear how the paused Pol II is released in response to stimulation.

198 f genes stimulates the subsequent release of paused pol II needed for transcription elongation.

199 letion reduces gene expression and increases paused pol II occupancy.

200 egulated enhancers attenuates the release of paused Pol II on PAF1 target genes without major interfe

201 king transcription factors, PcG proteins and paused Pol II states, these data identify a two-step mec

202 s human super elongation complexes (SECs) to paused Pol II to overcome this restriction.

203 ausing of RNA polymerase II at the promoter (paused Pol II) has emerged as a widespread and conserved

204 d by CBP and GAGA factor have high levels of paused Pol II, a unique chromatin signature, and are hig

205 in reduction in PcG binding, the release of paused Pol II, increases in promoter H3K4me3 histone mar

206 rotein (CBP) in regulating promoter-proximal paused Pol II.

207 py most promoter-proximal regions containing paused Pol II.

208 This shows that CDK9 stimulates release of paused polymerase and activates transcription by increas

209 (P-TEFb) to phosphorylate and activate this paused polymerase.

210 y step in the fabrication process is a print-pause-print approach for integrating membranes directly

211 ive initial transcription and for additional pausing prior to escape.

212 increases CSB processivity by decreasing the pausing probability during translocation.

213 the result of PAF1 loss releases Pol II from paused promoters of nearby PAF1 target genes.

214 iting for Pol II recruitment to these highly paused promoters through an interaction with TFIIB but f

215 ribosylation sites on NELF-E promotes Pol II pausing, providing a clear functional link between PARP-

216 ma-H2A at sites of Sgs1 binding, replication pausing regions, and long genes.

217 gmental area neurons exhibit longer aversive pauses relative to SNc neurons.

218 area neurons tend to exhibit longer aversive pauses relative to SNc neurons.SIGNIFICANCE STATEMENT Ou

219 e, increased burst size, decreased number of pauses), relative to fructose, across training.

220 1-BRD4 interaction to broadly promote RNAPII pause release and drive angiogenesis.Promoter proximal R

221 The biological roles of RNAPII pause release and the mechanisms by which extracellular

222 nctional role for H3K9ac in promoting pol II pause release by directly recruiting the super elongatio

223 Here we show that VEGF stimulates RNAPII pause release by stimulating acetylation of ETS1, a mast

224 Although elongation factors promote pause release leading to transcription elongation, the r

225 cing its binding to BRD4, which recruits the pause release machinery and increases RNAPII pause relea

226 at both genes and enhancers, suggesting that pause release may be widely inhibited during the celastr

227 n histone H3 is necessary for maximal pol II pause release through SEC action, and loss of H3K9ac inc

228 pon heat shock that are largely modulated at pause release, and HSF1 plays a limited and specialized

229 scription factor ETS1 promotes global RNAPII pause release, and that this process is governed by VEGF

230 ription initiation, promoter-proximal Pol II pause release, and transcription termination; however, m

231 complex, which functions in RNA polymerase 2 pause release.

232 nase CDK9, which is implicated in polymerase pause release.

233 heir expression by broadly increasing RNAPII pause release.

234 phosphorylation of Pol II is dispensable for pause release.

235 iated through reduction of RNA polymerase II pause release.

236 transcription and thus cannot rely solely on pause release.

237 pause release machinery and increases RNAPII pause release.

238 We identified transcription pause-release and elongation factors as one set of in vi

239 bodies, but not at promoters, suggestive of pause-release defects.

240 on levels are often achieved through a novel pause-release mechanism driven by high polymerase II ini

241 ys through enhancer-mediated transcriptional pause-release, promoting cell survival specifically in v

242 pid gene activation is linked to conditional pause-release.

243 phosphorylates Ser2-CTD and regulates RNAPII pause-release.

244 urkinje cells that acquire a precisely timed pause response that drives the overt blink response.

245 e found that Purkinje cells can learn double pause responses, separated by an intermediate excitation

246 In order for EF-P to associate with paused ribosomes, certain tRNAs with specific d-arm resi

247 pbl co-localize at gene promoters containing paused RNA polymerase 2, and Integrator similarly regula

248 hat GAF acts upstream of promoter-proximally paused RNA polymerase II (Pol II) formation (likely at t

249 ies nascent transcripts in promoter-proximal paused RNA polymerase II (Pol II).

250 Release of promoter-proximally paused RNA polymerase II (RNAPII) is a recently recogniz

251 iciently initiate transcription but generate paused RNA polymerase II downstream from the start site.

252 The release of paused RNA polymerase II into productive elongation is h

253 y show that the release of promoter-proximal paused RNA polymerase into elongation functions as a cri

254 cleotide addition and translocation by a non-paused RNA polymerase.

255 igh-occupancy TP53 enhancers, high levels of paused RNA polymerases, and accessible chromatin.

256 ed unwrapping, whereas inhibiting release of paused RNAPII or reducing RNAPII elongation decreased un

257 onal enhancers might modulate the release of paused RNAPII via 3D chromatin looping.

258 ed transduction of VEGF signaling to release paused RNAPII.

259 sigma70 binding and the elemental pause sequence together, but neither alone, produce a su

260 bilized, scrunched complex is the 'elemental pause sequence' recognized from its frequent occurrence

261 correlated with the presence of a bacterial pausing sequence motif, with reduced SNP density, and wi

262 esponsible for recognition of the trp operon pause signals.

263 stinct phases: activation, translocation and pausing, similar to ACF.

264 We show that the actual pause site in the stabilized, scrunched complex is the '

265 on results in "dribbling" of Pol II from the pause site to positions further downstream but impedes t

266 This pausing site correlated with the presence of a bacterial

267 nds revealed a novel conserved transcription pausing site near the light-strand TIS.

268 g codons can be as biologically important as pause sites in coordinating cotranslational folding.

269 These two regulatory pause sites participate in transcription attenuation and

270 f RNA Polymerase II from a proximal promoter paused state is a rate-limiting event in human gene cont

271 stimulation was aversive, and instrumentally pausing stimulation could reinforce lever-pressing.

272 Uniquely, the pause-stimulatory activity of Gfh proteins depends on th

273 its interactions, explaining why Nun acts on paused TECs.

274 Liquid reagents were integrated by briefly pausing the printing before resuming for sealing the dev

275 This strategy enables pausing the virus entry process at a specific stage and

276 Midbrain dopamine neurons recorded in vivo pause their firing in response to reward omission and av

277 Unlike animals, plants can pause their life cycle as dormant seeds.

278 ly, shortened synaptically evoked inhibitory pauses, thereby demonstrating the involvement of A-type

279 t that KOW5 is involved in promoter-proximal pausing through contact with the nascent RNA.

280 We fit the model directly to the acquired pause-time and run-time distributions.

281 cal rheology, we quantify real-time platelet pause times and translocation velocities across a Cu(2+)

282 d under shear stress, platelet translocation pause times on collagen-bound A1A2A3 are either normal o

283 esis of chronic kidney disease have given us pause to reconsider the current "glomerulocentric" parad

284 advancement in endophyte ecology warrants a pause to synthesize our understanding of endophyte disea

285 d mechanisms, ranging from RNA Polymerase II pausing to cotranscriptional histone modifications.

286 their capacity to restore promoter-proximal pausing to DSIF-depleted Drosophila nuclear extracts.

287 ol II, thereby transiting transcription from pausing to elongation.

288 g the gate loop displays moderate defects in pausing, transcript cleavage, and termination, it is ful

289 While the initiation of pauses typically involves synaptic or modulatory input,

290 hell is dominated by synchronized bursts and pauses, whereas signaling is uniform for core-projecting

291 eak upstream exons through RNA polymerase II pausing, whereas 5-methylcytosine evicts CTCF, leading t

292 atal pathway causes synchronous FSI activity pauses, which allow a transient window of disinhibition

293 elongation of RNAPs is often interrupted by pauses, which has been observed to cause RNAP traffic ja

294 verall termination efficiency by stimulating pausing, which increases the flux of ECs into the termin

295 cessing events and linked to transcriptional pausing, which is released by Bre5-Ubp3 associated with

296 els tend to display Pol II promoter-proximal pausing, while Pol II recruitment and Pol II pausing are

297 te stage-specific gene expression and Pol II pausing will contribute to our continuous search for nov

298 incidence of atrioventricular block or sinus pause with ozanimod.

299 near the transcription start site influence pausing, with divergent features between mammals and Dro

300 activated by scenarios involving stopping or pausing, yet evidence that the STN causally implements s