ライフサイエンスコーパス: pectate

1 tions, but the Pel fragment bound to calcium pectate, a major constituent of the plant cell wall.

2 , the primary sequence alignment between the pectate and pectin lyase subfamilies has been corrected

3 atively charged pectin to create an extensin pectate coacervate that may template further orderly dep

4 ordered and represent an atomic view of the pectate component in plant cell walls.

5 thogen Erwinia chrysanthemi and degrades the pectate component of plant cell walls in soft rot diseas

6 ity (anhydrouronic acid, methoxy and calcium pectate contents and degree of esterification).

7 The conformation of the pectate fragment is a mix of 21 and 31 right-handed heli

8 he pel gene from an Amycolata sp. encoding a pectate lyase (EC 4.2.2.2) was isolated by activity scre

9 winia carotovora secrete several isozymes of pectate lyase (Pel) by the out-encoded type II pathway.

10 es showing sequence homology to higher-plant pectate lyase (Pel) genes were isolated from ripening ba

11 uppress host defense responses, and secretes pectate lyase (Pel) to degrade the plant cell wall.

12 ora subsp. carotovora produces extracellular pectate lyase (Pel), polygalacturonase (Peh), cellulase

13 tococca, a plant pathogen, has two inducible pectate lyase (PL) genes (pel) encoding PL that can help

14 erase (PME), pectin acetylesterase (PAE) and pectate lyase (PL) where all highly expressed and latex-

15 hat the production of extracellular enzymes (pectate lyase [Pel], polygalacturonase [Peh], cellulase

16 we report a gene for a cellulosomal subunit, pectate lyase A (PelA), lying downstream of the engY gen

17 a significant increase in calcium-dependent pectate lyase activity during ripening.

18 el gene in S. lividans TK24 resulted in high pectate lyase activity in the culture supernatant, conco

19 Pectate lyase activity is detected in elongating and dif

20 No pectate lyase activity was detected in E. coli BL21 with

21 Although no pectate lyase activity was detected, the C-terminal regi

22 The purified LjNPL protein had pectate lyase activity, demonstrating that this activity

23 el clone produced a recombinant protein with pectate lyase activity, demonstrating that this sequence

24 Escherichia coli possesses calcium-dependent pectate lyase activity.

25 e show that two pectin modification genes, a pectate lyase and pectinesterase, are targets of both bH

26 The folding mechanism of pectate lyase C (pelC) involves two slow phases that hav

27 Pectate lyase C (pelC) is a member of the class of prote

28 mensional structure of a complex between the pectate lyase C (PelC) R218K mutant and a plant cell wal

29 Pectate lyase C (pelC) was the first protein in which th

30 n with the structure of Erwinia chrysanthemi pectate lyase C (PelC), the primary sequence alignment b

31 or in vitro activity of Erwinia chrysanthemi pectate lyase C (PelC).

32 When modeled based on pectate lyase C of Erwinia chrysanthemi, the RHbetaH of

33 he first avirulence gene and determined that pectate lyase C possessed a novel structural motif, know

34 The Amycolata pectate lyase clearly belongs to the pectate lyase super

35 The Amycolata pectate lyase contains a signal peptide of 26 amino acid

36 ase family, in contrast to that found in the pectate lyase enzymes.

37 High xylanase or pectate lyase expression was observed when C. cellulovor

38 uronate lyases (OGLs; now also classified as pectate lyase family 22) are cytoplasmic enzymes found i

39 e invariant and conserved amino acids in the pectate lyase family of proteins.

40 bserved between the cellular distribution of pectate lyase folding and the distinct metal coordinatio

41 harpins have C-terminal enzyme-like domains: pectate lyase for HopAK1 and lytic transglycosylase for

42 with PelE from Erwinia chrysanthemi and the pectate lyase from Glomerella cingulata.

43 inal amino-acid sequence match the wild-type pectate lyase from the Amycolata sp.

44 ere we identify a Lotus japonicus nodulation pectate lyase gene (LjNPL), which is induced in roots an

45 re have been no reports available to date on pectate lyase genes from Clostridia.

46 orial extracts and high expression levels of pectate lyase genes suggest that the parasite contribute

47 cc71) produces extracellular enzymes such as pectate lyase isozymes (Pels), cellulase (Cel), polygala

48 mutants displayed a significant reduction in pectate lyase production, a virulence factor of this bac

49 ng that this sequence was likely to encode a pectate lyase protein in planta.

50 l reactive arginine, analogous to the pectin/pectate lyase reaction site, is accessible to the solven

51 In contrast, PelE, a pectate lyase secreted via the type II protein secretion

52 that an arginine, which is invariant in the pectate lyase superfamily, is the amino acid that initia

53 ycolata pectate lyase clearly belongs to the pectate lyase superfamily, sharing all functional amino

54 hlI (gene for AHL synthase), pel-1 (gene for pectate lyase), or rsmB (gene for regulatory RNA that bi

55 alin A), and parallel beta-helical proteins (pectate lyase).

56 One of these cDNAs shows homology to pectate lyase, a pectin-degrading enzyme.

57 ) for the bHLH transcription factors and the pectate lyase, but not for the pectinesterase, complemen

58 gh levels of the exoenzyme virulence factors pectate lyase, cellulase and protease.

59 ta-helix as seen in the pectinolytic enzymes pectate lyase, pectin lyase, polygalacturonase and rhamn

60 The basal levels of extracellular pectate lyase, polygalacturonase, and cellulase as well

61 FlhDC(-) mutant produces very low levels of pectate lyase, polygalacturonase, cellulase, protease, a

62 rate-binding clefts of polygalacturonase and pectate lyase, which bind and cleave the same substrate,

63 e in a glycosylphosphatidylinositol-anchored pectate lyase-like gene) exhibited a strong increase in

64 a previously described member of a family of pectate lyase-like genes.

65 PMR6 encodes a pectate lyase-like protein with a novel C-terminal domai

66 re we report direct interaction of NPG1 with pectate lyase-like proteins (PLLs).

67 e active site and substrate-binding cleft of pectate lyase.

68 of ripening, by silencing a gene encoding a pectate lyase.

69 combined activities of polygalacturonase and pectate lyase.

70 ough the action of pectin-methylesterase and pectate-lyase that possibly originated from a microbial

71 nd Erwinia carotovora secrete extra-cellular pectate lyases (Pels) using the type II or Out pathway.

72 protein with domains resembling harpins and pectate lyases (Pels), respectively.

73 nearly identical to that found in the pectin/pectate lyases from several plant pathogenic microorgani

74 e C-terminal region of HrpW is homologous to pectate lyases of a unique class, suggesting that HrpW m

75 l DNA libraries more than 40 novel microbial pectate lyases were discovered, and their enzymatic prop

76 rwinia chrysanthemi belonging to family 1 of pectate lyases, a putative cellulose-binding domain, a c

77 E. chrysanthemi that belongs to family 4 of pectate lyases, and a duplicated sequence (or dockerin)

78 xpressed in mature pollen, shows homology to pectate lyases, and is the putative homologue of the tom

79 e distinct metal coordination chemistries of pectate lyases.

80 ructure and function of Erwinia chrysanthemi pectate lysase C, a plant virulence factor, is reviewed