ライフサイエンスコーパス: peptide N-glycosidase

1 ide and the peptide moieties are released by peptide-N-glycosidase.

2 Controlled deglycosylation using peptide : N-glycosidase F and endo-beta-N-acetylglucosam

3 saccharide mapping, bands were digested with peptide N-glycosidase F (PNGase F) in order to release t

4 ting membrane preparations were treated with peptide N-glycosidase F (PNGase-F).

5 Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues f

6 Moreover, treatment with peptide N-glycosidase F abrogated F240 neutralization, i

7 Peptide N-glycosidase F and endoglycosidase H deglycosyl

8 igestion with Flavobacterium meningosepticum peptide N-glycosidase F and trypsin, with matrix-assiste

9 PSA on NRP-2 is resistant to digestion with peptide N-glycosidase F but is sensitive to release unde

10 -1,4-galactosyltransferase or susceptible to peptide N-glycosidase F corresponded directly to their r

11 ther endoglycosidase F or H but sensitive to peptide N-glycosidase F digestion.

12 Pretreatment of the lactoferrin with peptide N-glycosidase F or addition of heparin or chondr

13 removal of the oligosaccharide chains using peptide N-glycosidase F or removal of the glucoses by ER

14 d ABCG2 are glycosylated, and treatment with peptide N-glycosidase F reduces the apparent molecular m

15 Treatment of kidney membrane proteins with peptide N-glycosidase F showed that GLUT9 and GLUT9Delta

16 Etanercept was first treated with peptide N-glycosidase F to release the N-glycans.

17 N-Glycosylation was responsible because peptide N-glycosidase F treatment of isolated 170-kDa EG

18 Peptide N-glycosidase F, but not endoglycosidase H, dige

19 zymatically released from glycoproteins with peptide N-glycosidase F, followed by purification with g

20 of 7.5 h and was sensitive to treatment with peptide N-glycosidase F, sialidase alone, or sialidase a

21 ATPase was examined by using tunicamycin and peptide N-glycosidase F, two agents used to prevent and

22 of the cells with trypsin, endoglycosidase F/peptide N-glycosidase F, Vibrio cholerae neuraminidase,

23 Using mass spectrometry on purified and peptide N-glycosidase F-deglycosylated CD36 and also by

24 f approximately 160 kDa after treatment with peptide N-glycosidase F.

25 ecifically inhibited by deglycosylation with peptide N-glycosidase F.

26 Deglycosylation of the receptor with peptide N:glycosidase F results in a decrease in molecul

27 formerly N-linked glycosylated peptides via peptide- N-glycosidase F (PNGase F).

28 not recognized by the classical glycosidases peptide-N-glycosidase F (PNGase F) and endoglycosidase H

29 specificities of endoglycosidases such as a peptide-N-glycosidase F (PNGase F) and of endo-N-acetlyg

30 Changes in electrophoretic mobility after peptide-N-glycosidase F (PNGase F) digestion suggest tha

31 A novel online enzyme reactor incorporating peptide-N-glycosidase F (PNGase F) on a monolithic polym

32 PO antibodies, dual digestion by trypsin and peptide-N-glycosidase F (PNGase F), and analysis by LC-M

33 transfected with GPVI, deglycosylation with peptide-N-glycosidase F (PNGase F; specific for complex

34 A deglycosylation step using Peptide-N-Glycosidase F (PNGaseF) has been introduced in

35 inked monosaccharides based on resistance to peptide-N-glycosidase F and analysis of saccharides rele

36 Deglycosylation using peptide-N-glycosidase F and site-directed mutagenesis es

37 Peptide-N-glycosidase F digestion of solubilized hKOR or

38 N-deglycosylation by peptide-N-glycosidase F digestion resolved these isoform

39 on analyses of Man(6)GlcNAc(2) released with peptide-N-glycosidase F from invertase secreted by Delta

40 After a simple and fast incubation using peptide-N-glycosidase F on target, sequential mass shift

41 Treatment with peptide-N-glycosidase F reduced the size of the mammalia

42 Treatment of AGMK and cr5 cell extracts with peptide-N-glycosidase F resulted in the collapse of the

43 Treatment of heart lysates with peptide-N-glycosidase F revealed that while giant obscur

44 were glycoproteins and molecular mass after peptide-N-glycosidase F treatment was 38 and 45 kDa, res

45 Both trifluoromethanesulfonic acid and peptide-N-glycosidase F treatments yielded a 50-kDa band

46 eleased from the antibody via digestion with peptide-N-glycosidase F, then derivatized with a charged

47 hy following release from the polypeptide by peptide-N-glycosidase F.

48 after deglycosylation with endoglycosidase F/peptide-N-glycosidase F.

49 in Xenopus oocytes and deglycosylation with peptide-N-glycosidase F.

50 hrough and bound fractions, and treated with peptide: N-glycosidase F to remove N-linked glycans.

51 rescent protein-tagged mCLCA6 with PNGase F (peptide: N-glycosidase F) to remove N-linked glycosyl gr

52 sing endo-beta-N-acetylglucosaminidase H and peptide:N-glycosidase F sensitivity assays on CDKAL1 con

53 proximately 120 kDa following treatment with peptide:N-glycosidase F, consistent with N-glycosylation

54 ological approaches, including use of either peptide:N-glycosidases F and A (PNGase F and A) or anhyd

55 purified enzyme could be deglycosylated with peptide N-glycosidase-F to a core molecular mass of 54 k

56 osylation of the full-length homotrimer with peptide N-glycosidase-F under native conditions abolishe

57 teins using sequential endoglycosidase H and peptide:N-glycosidase-F digestions.

58 ds were comparable to profiling by PNGase F (peptide N-glycosidase from Flavobacterium meningosepticu

59 [35S]methionine labeling of BRL-3A cells, a peptide:N-glycosidase-sensitive protein (45 kDa) was obs

60 Endoglycosidase H and peptide N-glycosidase treatment and cell surface immunop