ライフサイエンスコーパス: peptide mapping

1 spectrometry, ion mobility, and quantitative peptide mapping.

2 tion signals as determined by phosphotryptic peptide mapping.

3 ool to increase sequence coverage in tryptic peptide mapping.

4 esis-electrospray ionization (CE-ESI)-TOF MS peptide mapping.

5 by peptide sequencing and mass spectrometric peptide mapping.

6 residue reactivity with dithiodipyridine and peptide mapping.

7 ing to the partial amino acid sequences from peptide mapping.

8 n, followed by proteolytic and immunological peptide mapping.

9 and liquid chromatography/mass spectrography peptide mapping.

10 of DNA-dependent protein kinase (DNA-PK) by peptide mapping.

11 ee-disulfide species have been identified by peptide mapping.

12 gelsolin by amino acid sequencing following peptide mapping.

13 spectrometry and glycation sites located by peptide mapping.

14 ein variants that are difficult to detect by peptide mapping.

15 ing truncated GBS-PGK molecules, followed by peptide mapping.

16 ation was investigated by mass spectrometric peptide mapping.

17 uid chromatography-mass spectrometry (LC-MS) peptide mapping.

18 residue preceding lysine 222, determined by peptide mapping.

19 n vivo by [(32)P]orthophosphate labeling and peptide mapping.

20 ion by mass spectrometry and two-dimensional peptide mapping.

21 2-amino acid motif in the N-terminal CbpA by peptide mapping.

22 ve-like conformation was further verified by peptide mapping after limited trypsin proteolysis, and b

23 y mass (10000.5 Da) and amino acid sequence (peptide mapping after proteolysis) determined by matrix-

24 n sites of carboxylase by mass spectrometric peptide mapping analyses combined with site-directed mut

25 l and complementary technique to RPLC-MS for peptide mapping analyses of antibody-drug conjugates (AD

26 tified as casein kinase Ialpha (CKIalpha) by peptide mapping analysis and sequencing.

27 reduced by 60, 7, and 96%, respectively, and peptide mapping analysis of the mutant enzymes confirmed

28 as a result of molecular mass determination, peptide mapping analysis, and MS/MS sequencing.

29 ered as a result of intact mass measurement, peptide mapping analysis, and tandem mass spectroscopy s

30 Following peptide mapping analysis, significant amounts of asparty

31 ization on the isolated glycated material by peptide mapping analysis, using liquid chromatography-ma

32 Detailed tryptic peptide mapping analysis, using MALDI/TOF-MS, identified

33 ns) were phosphorylated in vivo, and tryptic peptide-mapping analysis suggested a single, similar pho

34 Bottom-up characterization using RP-HPLC/MS peptide mapping and accurate mass measurements identifie

35 ication was determined to be Tyr-236 by CNBr peptide mapping and automated peptide sequencing.

36 and the antigenic structure of B5R(275t) by peptide mapping and by reciprocal MAb blocking studies u

37 rimary structure of mefp-5 was determined by peptide mapping and cDNA sequencing.

38 nized with HCV-1 rE1E2 was conducted through peptide mapping and competition studies with a panel of

39 of high mass accuracy in mass spectrometric peptide mapping and database searching, selected protein

40 We used peptide mapping and epitope-specific immunoprecipitation

41 Subsequent trapping, proteolysis, peptide mapping and fragmentation by mass spectrometry,

42 MS proteolytic peptide mapping and genome database searching provide a

43 ed laser desorption-ionization (MALDI) TOFMS peptide mapping and intact MW so that a standard map is

44 Tryptic peptide mapping and MALDI-MS verify labeling at the engi

45 was demonstrated by two-dimensional tryptic peptide mapping and mass analysis to be either threonine

46 -inactivated CYP3A4(His)(6) followed by HPLC-peptide mapping and mass spectrometric (LC/MS/MS) analys

47 tion of various deamidated forms followed by peptide mapping and mass spectrometric analyses revealed

48 By peptide mapping and mass spectrometric analysis of a sol

49 Preliminary peptide mapping and mass spectrometry analysis suggest t

50 direct phosphorylation in vitro followed by peptide mapping and mass spectrometry sequencing.

51 Peptide mapping and matrix-assisted laser desorption ion

52 ported by quantitative results acquired from peptide mapping and methylamine labeling.

53 using the traditional bottom-up approach of peptide mapping and MS sequencing methodologies, two DMP

54 Peptide mapping and mutagenesis studies have also shown

55 vitro by ERK1, JNK and p38, and confirmed by peptide mapping and mutagenesis that Thr53 is phosphoryl

56 Using tryptic peptide mapping and mutagenesis, we have identified seri

57 Peptide mapping and mutational analyses localized the bu

58 e element of the GLUT1 ATP binding domain by peptide mapping and N-terminal sequence analysis of prot

59 Mass spectrometry-based peptide mapping and N-terminal sequencing demonstrated t

60 Peptide mapping and N-terminal sequencing has been used

61 Peptide mapping and phosphatase diagests showed that the

62 Further peptide mapping and sequence analysis of CB11 revealed e

63 Peptide mapping and sequencing data indicate that the pa

64 We report the first peptide mapping and sequencing of an in vivo isolevuglan

65 We used two-dimensional peptide mapping and sequencing to identify three residue

66 Furthermore, by peptide mapping and site-directed mutagenesis we demonst

67 Using peptide mapping and site-directed mutagenesis, we have i

68 Peptide mapping and structural analysis by Fourier trans

69 Peptide mapping and subsequent mass spectrometry analysi

70 Tryptic peptide mapping and tandem mass sequencing were used to

71 Tryptic peptide mapping and tandem mass spectrometry of the redu

72 By combining proteolytic digestion with peptide mapping and tandem mass spectrometry techniques,

73 aphy, were fully characterized using tryptic peptide mapping and tandem mass spectrometry.

74 ction/alkylation of the protein, followed by peptide mapping and tanden mass spectrometry (MS/MS) seq

75 Immunoreactive epitopes were searched for by peptide mapping, and 171 cleavable, biotinylated 17-mer

76 irected mutagenesis, chemical cross-linking, peptide mapping, and LC-MS/MS analyses.

77 complex with trypsin, followed by isolation, peptide mapping, and mass spectrometric and tandem mass

78 mance liquid chromatography, two-dimensional peptide mapping, and matrix-assisted laser desorption/io

79 ies of VEGF-D using a neutralizing antibody, peptide mapping, and mutagenesis to demonstrate that the

80 se serines, based on in vitro kinase assays, peptide mapping, and mutational analysis.

81 Using a combination of receptor mutagenesis, peptide mapping, and N-terminal sequencing, we identifie

82 (ESI-q-TOF-MS), N-terminal Edman sequencing, peptide mapping, and other techniques.

83 with monoclonal antibodies, one-dimensional peptide mapping, and partial amino acid sequencing demon

84 so subjected to proteolysis, two-dimensional peptide mapping, and phosphoamino acid analysis.

85 were identified by purification, proteolytic peptide mapping, and radiochemical sequencing of labeled

86 by absorbance and fluorescence spectroscopy, peptide mapping, and sequence analysis.

87 nces within EC2 and N terminus identified by peptide mapping are in close proximity in the equilibriu

88 ction of activated B-cells was identified by peptide mapping as alpha-tubulin.

89 n-labeled RTPR with endoproteinase Glu-C and peptide mapping at pH 5.8 revealed that C419 was predomi

90 inhibitor could no longer be detected after peptide mapping at this site or at the catalytic site.

91 By peptide mapping, automated sequencing, and mass spectrom

92 Two synthetic peptides mapping basic clusters of the cytosolic sClC3-C

93 Peptide mapping benefits from an efficient front-end sep

94 tics of the ABX and rituximab in AR160 using peptide mapping/Biacore approach.

95 Peptide mapping by high performance liquid chromatograph

96 We used peptide mapping by HPLC, Edman sequencing, and matrix-as

97 eins were digested with cyanogen bromide and peptide mapping by LC-MS was established.

98 Peptide mapping by matrix-assisted laser desorption/ioni

99 gel electrophoresis with in-gel proteolysis, peptide mapping by MS, and sequence database searches fo

100 of cross-linking were determined by tryptic peptide mapping by using time-of-flight MS.

101 he potential to be applied under the general peptide mapping conditions.

102 d fluorescence detection, the sensitivity of peptide mapping could be improved 2000 times compared to

103 Peptide mapping coupled with mass spectrometric analyses

104 d after limited proteolysis was confirmed by peptide mapping coupled with tandem mass spectrometry an

105 HPLC-ESI-MS peptide mapping data demonstrated that the purified muri

106 m protein databases using mass spectrometric peptide mapping data.

107 p (Glu(345)), as demonstrated by proteolytic peptide mapping, deglycosylation, micropurification, and

108 Peptide mapping demonstrated that both TGF-beta 1 and p3

109 Here we describe a simple approach to peptide mapping designed for large sample sets that incl

110 Peptide mapping determined a minimum of five conserved e

111 pectrometry (CZE-MS) has great potential for peptide mapping due to high efficiency and outstanding s

112 ressed in COS-1 cells using a combination of peptide mapping, Edman degradation, and mass spectrometr

113 lytical methods such as amino acid analysis, peptide mapping, electrospray mass spectrometry, and Edm

114 ve approach for protein characterization via peptide mapping employing a data independent LC-MS acqui

115 as well as other information relevant to the peptide mapping experiment.

116 Peptide mapping experiments identified the mAb 2E1 cross

117 Two-dimensional peptide mapping experiments showed that one of the Cx45.

118 Our peptide mapping experiments showed that the Ser(363) sit

119 However, despite this considerable homology, peptide-mapping experiments also revealed that immunodom

120 munodepletion, in vitro phosphorylation, and peptide-mapping experiments indicated that Cdc2 is likel

121 Peptide mapping followed by sequence analysis revealed t

122 s labeled with lutetium-177 and subjected to peptide mapping followed by sequence analysis.

123 include CE as a complement to reverse-phase peptide mapping for the identification of small peptides

124 tion of the analytical artifact during LC-MS peptide mapping for the measurement of Met sulfoxide.

125 ns in linear MALDI/MS and was reconfirmed by peptide mapping for three of the proteins.

126 Although mass spectrometric peptide mapping has become an established technique for

127 labeling, trypsin digestion, two-dimensional peptide mapping, high performance liquid chromatography,

128 ct association between the two proteins, and peptide mapping identified an ERK2 binding site within t

129 Mass spectrometry peptide mapping identified arginine-18 as the hotspot si

130 Peptide mapping identified one disulfide bond between Cy

131 Peptide mapping identified two acidic clusters in DMP1 r

132 s the determination of such heterogeneity by peptide mapping in both the heavy chain and the light ch

133 Peptide mapping in conjunction with mass spectrometry sh

134 as further complemented experimentally using peptide mapping in tandem with mass spectrometry and sit

135 structure of the receptor was determined by peptide mapping in the absence and presence of reducing

136 artic acid and 80% aspartic acid detected by peptide mapping in the degraded sample (8 weeks, 45 degr

137 dified minimally with methylglyoxal, tryptic peptide mapping indicated a hotspot of modification at A

138 Peptide mapping is a useful technique for identifying po

139 The most commonly used method for peptide mapping is based on reverse phase liquid chromat

140 Liquid chromatography (LC)-based peptide mapping is extensively used for establishing pro

141 omatography with mass spectrometry (RPLC-MS) peptide mapping is routinely used for interrogating mole

142 LC-MS/MS peptide mapping is well suited to the discovery and quan

143 ng, and reduce cost and preparation time, of peptide mapping LC-MS workflows in protein analytical re

144 The automated peptide mapping LC/MS system has great utility in prepar

145 A completely automated peptide mapping liquid chromatography/mass spectrometry

146 Peptide mapping mass spectrometry indicated that host-sp

147 ied with fucose and through a combination of peptide mapping, mass spectrometry, and sequence analysi

148 In the work reported here, we used peptide mapping, mass spectrometry, and site-directed mu

149 Here we used peptide mapping, mass spectroscopy analysis, and mutagen

150 a high-resolution, high-sensitivity LC-UV-MS peptide mapping method for the therapeutic antibody, tra

151 A highly sensitive peptide mapping method using derivatization and fluoresc

152 MAM is a peptide mapping method utilizing mass spectrometry to de

153 ns accurately, an isotope labeling and LC-MS peptide mapping method was developed.

154 The CZE-MS peptide mapping method with the modified BGE produced si

155 mine, by a sodium borohydride-dependent mass peptide mapping method, the galactation sites in HSA; an

156 Both LC-MS and peptide mapping methodologies were found to be useful in

157 This study showed that the new peptide mapping methodology with a combination of mass s

158 e chromophores were further located by a new peptide mapping methodology with a combination of mass s

159 The peptide mapping methods developed for characterization a

160 However, current LC-UV/MS peptide mapping methods require multiple analyses and MS

161 ific modification with 4-vinylpyridine, HPLC peptide mapping methods, and mass spectrometry to analyz

162 lytic (lysylendopeptidase-C) digestion, HPLC-peptide mapping, microEdman sequencing, and mass spectro

163 Classical peptide mapping narrowed the major phosphorylation site

164 drug-target interaction network and protein-peptide mapping network.

165 Peptide mapping of >80% of the residues was accomplished

166 Peptide mapping of 3H-methyl-labeled H3 indicated methyl

167 Peptide mapping of a 20 fmol amount of tagged digest was

168 s regions to the immune system was tested by peptide mapping of antiserum specificities against sets

169 hogonal technique with growing attention for peptide mapping of biotherapeutic proteins due to its hi

170 er516 was confirmed by tryptic digestion and peptide mapping of COX-2 labeled with [1-14C-acetyl]sali

171 Tryptic digestion and peptide mapping of COX-2 reacted with [1-14C-acetyl]-36

172 By two-dimensional tryptic peptide mapping of immunoprecipitated NHE-1, we identify

173 Tryptic peptide mapping of in vivo labeled IRS-1 and the S612A m

174 Using two-dimensional peptide mapping of in vivo radiolabeled p53 tryptic phos

175 Peptide mapping of labeled wild-type and mutant receptor

176 Peptide mapping of mono(3'-dADP-ribosyl)ated-PARP follow

177 The system was used for peptide mapping of monoclonal antibodies (mAbs), known a

178 1) and Cys(32)-Cys(39) by protease-generated peptide mapping of partially reduced and S-alkylated rSM

179 A novel multimodal method for peptide mapping of proteins by multiplexed capillary ele

180 e-of-flight mass spectrometry (MALDI-TOF MS) peptide mapping of proteins isolated by PAGE.

181 identification of phosphopeptides from HPLC peptide mapping of proteolytic digests of phosphoprotein

182 Moreover, peptide mapping of sera from animals receiving cross-lin

183 In vitro PKA phosphorylation and tryptic peptide mapping of SNS and mutant SNS(SA) I-II loops exp

184 proved mixing experiments and by comparative peptide mapping of specific polypeptides recovered from

185 nto two subgroups based on serological data, peptide mapping of the coat protein, nucleic acid hybrid

186 Peptide mapping of the Cu2+-inactivated enzyme revealed

187 Peptide mapping of the irreversibly bound heme adduct in

188 for differences in Km and thermal stability, peptide mapping of the LDH-As of all six species was fir

189 to be proximal to the major groove of DNA by peptide mapping of the region of TBP cross-linked at bp

190 Peptide mapping of the tagged digest reviews a larger nu

191 The peptide mapping of the tagged digest was conducted with

192 Peptide mapping of the tBPA-modified protein provides ev

193 Comparative peptide mapping of these B15 allotypes further pinpoints

194 Peptide mapping of tryptic digests of the inactivated CY

195 Using peptide mapping of tryptic digests, LC/MS, and amino aci

196 During peptide mapping of unalkylated hemoglobins with Staphylo

197 Protein microsequencing and peptide mapping of wild-type and mutant fusion proteins

198 from an isolated protein followed by either peptide mapping or tandem MS (MS/MS) to obtain sequence

199 yed included various proteolytic digestions, peptide mapping, partial reduction, and assignment of di

200 Consistent with the reconstructions, peptide mapping places the ubiquitin linkage on lysine 1

201 A new peptide mapping procedure, incorporating derivatization

202 s with this new method compared to a typical peptide mapping procedure.

203 is, and data interpretation than traditional peptide mapping procedures.

204 g is monitored using mass spectrometry-based peptide mapping, providing spatially resolved measuremen

205 pha 2(I) chains as determined by V8 protease peptide mapping, reached the highest intracellular level

206 Our peptide mapping results provide a more detailed structur

207 Detailed peptide mapping revealed as many as seven covalent cross

208 Peptide mapping revealed that ferritin binds to a 22-aa

209 Peptide mapping revealed that the modification occurred

210 present results from optimization of CZE-MS peptide mapping separation using mixed aqueous-aprotic d

211 The resulting proteins were characterized by peptide mapping, sequence analysis, and mass spectrometr

212 In the case of peptide mapping, several peptide masses are needed to un

213 Remarkably, peptide mapping showed most epitopes recognized by naive

214 Two-dimensional peptide mapping showed only a single phosphopeptide; two

215 dimensional gel electrophoresis and tryptic peptide mapping showed that entry into the nucleus resul

216 high performance liquid chromatography, and peptide mapping showed that it was the same in the two e

217 Peptide mapping showed that the alpha beta and (alpha be

218 Tryptic peptide mapping showed that the CpcSU-dependent reaction

219 Systematic peptide mapping showed that the site of interaction betw

220 mbination with mass spectrometry and tryptic peptide mapping showed unambiguously that RLF is larger

221 with FPR are consistent with cross-linking, peptide mapping, spectroscopic, and electron transfer da

222 Peptide mapping studies covering 88% of the deduced amin

223 Previous epitope and peptide mapping studies have also indicated that the PIL

224 Furthermore, peptide mapping studies indicated that BDV-P is phosphor

225 Southern blot and peptide mapping studies indicated that this 31-kDa antig

226 Peptide mapping studies of in vivo phosphorylated TXA2 r

227 Peptide mapping studies revealed that more positive reca

228 sult, taken together with the results of the peptide mapping studies, establishes that the site of Bp

229 rroborated the chemical modification and the peptide mapping studies, establishing the importance of

230 Peptide-mapping studies of IgG subclass responses identi

231 protein subunits using a high-accuracy mass peptide-mapping technique.

232 Peptide mapping, thiol titrations, UV-vis spectrophotome

233 This dimerization interface is validated by peptide mapping through hydrogen/deuterium exchange mass

234 psilon to the gamma subunit was localized by peptide mapping to a region of the gamma subunit between

235 used radioactive iodide labeling followed by peptide mapping to gain insight into the structure of P.

236 es, we used photo affinity cross-linking and peptide mapping to identify the substrate-binding sites

237 Ultimately, the proteins can be studied by peptide mapping to search for posttranslational modifica

238 In this study, we have used peptide mapping to study the oxidation kinetics of each

239 Peptides mapping to 2784 proteins in 1168 protein groups

240 sequence coverage by the number of distinct peptides mapping to each protein identification, the CIT

241 ssays was demonstrated against, or shown by, peptides mapping to the third and fourth predicted surfa

242 Subsequent peptide mapping using chemical and proteinase cleavages

243 e Ser/Thr kinase domain of PKCdelta based on peptide mapping using liquid chromatography/mass spectro

244 as identified as the phosphorylation site by peptide mapping using mass spectrometry, site-directed m

245 nium-containing fractions were identified by peptide mapping using nano LC-ESI/LTQMS.

246 Peptide mapping using proteolytic enzymes is one useful

247 Peptide mapping using Urea-PAGE followed by CA revealed

248 Peptide mapping was performed using high-resolution MS,

249 Peptide mapping was used to identify a PKA phosphorylati

250 Using two-dimensional peptide mapping, we demonstrate that peptides correspond

251 Using deletion mutagenesis and peptide mapping, we have identified the sequences in Cdc

252 study, mass spectrometry and two-dimensional peptide mapping were used to determine that tyrosines 22

253 ntages were evaluated through application to peptide mapping, wherein CSH C18 was found to aid the de

254 e alternative to conventional time-intensive peptide mapping which is prone to artificial oxidation d

255 with Girard's Reagent T (GRT) and subsequent peptide mapping with high-resolution mass spectrometry.

256 Peptide mapping with liquid chromatography-tandem mass s

257 Peptide mapping with mass spectrometry (MS) detection is

258 18O, and time point samples were analyzed by peptide mapping with mass spectrometry to measure the ra

259 a8 was isolated by column chromatography for peptide mapping with mass spectrometry.

260 pwise reduction and alkylation at acidic pH, peptide mapping with matrix-assisted laser desorption io

261 Its structure was elucidated by peptide mapping with multiple proteases with various spe

262 lonal antibody by LC-MS and nonreduced Lys-C peptide mapping with tandem mass spectrometry.

263 ing on the beta1 chain was localized by CNBr peptide mapping within residues 130-146, a region that c

264 s method adds just one step to the classical peptide mapping workflow.

265 phosphoserine as the putative PKA site, and peptide mapping yielded one phosphopeptide.