ライフサイエンスコーパス: peptide synthesis

1 ot horizontal lineTap-ketals for solid-phase peptide synthesis.

2 s compatible with Fmoc-chemistry solid-phase peptide synthesis.

3 resin by conventional Fmoc-based solid-phase peptide synthesis.

4 rotected analogue for Fmoc-based solid-phase peptide synthesis.

5 is compatible with standard Fmoc solid-phase peptide synthesis.

6 ntly suppresses aspartimide formation during peptide synthesis.

7 A caspase-3/7 substrate using solution-phase peptide synthesis.

8 ble of sequence-specific beta-homo (beta(3)) peptide synthesis.

9 ccupies the active site like a substrate for peptide synthesis.

10 dinary amino acids in Fmoc-based solid-phase peptide synthesis.

11 enzymatic sulfation, chemical sulfation and peptide synthesis.

12 ployed a new linker for Fmoc-based thioester peptide synthesis.

13 that is compatible with standard Fmoc-based peptide synthesis.

14 rated into peptides via standard solid-phase peptide synthesis.

15 n domain is necessary for factor-independent peptide synthesis.

16 antly shorter and comparable to that for any peptide synthesis.

17 ted into VM(11)VVQTK by standard solid-phase peptide synthesis.

18 s enzymology typically found in nonribosomal peptide synthesis.

19 opriately protected for Fmoc/Boc solid-phase peptide synthesis.

20 ion and are compatible with Fmoc solid phase peptide synthesis.

21 vitro without requiring intracellular viral peptide synthesis.

22 ized as a challenging target for solid-phase peptide synthesis.

23 thesized in a modular fashion by solid-phase peptide synthesis.

24 ta(2)-amino acids, which are useful for beta-peptide synthesis.

25 enylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis.

26 eloped from an early system of RNA-dependent peptide synthesis.

27 olid support in conjunction with solid-phase peptide synthesis.

28 s and 33 transfer RNAs of 19 amino acids for peptide synthesis.

29 N(alpha)-protection suitable for solid-phase peptide synthesis.

30 and applied to reactions used in solid-phase peptide synthesis.

31 rmediates to the various enzymes that effect peptide synthesis.

32 enylmethoxycarbonyl (Fmoc)-based solid-phase peptide synthesis.

33 ave and deprotect peptides after solid-phase peptide synthesis.

34 glycoamino acids can also serve as units for peptide synthesis.

35 the effect of altering the uORF sequence on peptide synthesis.

36 tance P analogue with the use of solid-phase peptide synthesis.

37 nd chemically incorporated using solid phase peptide synthesis.

38 JB3 was generated by solid phase peptide synthesis.

39 za B was synthesized by standard solid-phase peptide synthesis.

40 the aminoacyl-tRNA synthetases in ribosomal peptide synthesis.

41 ]-Thr-NH(2), 1) were prepared by solid-phase peptide synthesis.

42 e show here efficient 50S ribosome catalyzed peptide synthesis.

43 spectrometer was used to monitor solid-phase peptide synthesis.

44 to the peptides by standard Fmoc solid phase peptide synthesis.

45 storical development of RNA-based systems of peptide synthesis.

46 tion of TGFalpha transcription and increased peptide synthesis.

47 in I synthetase) that catalyse non-ribosomal peptide synthesis.

48 suggested as likely substrates for prebiotic peptide synthesis.

49 fat body, the primary organ of antimicrobial peptide synthesis.

50 y chromatography and prepared by solid phase peptide synthesis.

51 ntermediates for peptide ligation and cyclic peptide synthesis.

52 ) is a strong anion byproduct of solid-phase peptide synthesis.

53 readily used in subsequent Fmoc solid-phase peptide synthesis.

54 significant enough to form aggregates during peptide synthesis.

55 cid as a building block for Fmoc solid phase peptide synthesis.

56 utility of thioacids and oxidative dimers in peptide synthesis.

57 is an attractive alternative to solid-phase peptide synthesis.

58 tion of derivatives suitable for solid phase peptide synthesis.

59 oth the convergence and divergence of cyclic peptide synthesis.

60 terminus of a peptide segment by solid phase peptide synthesis.

61 have used them as units in Fmoc solid-phase peptide synthesis.

62 pounds are suitable for standard solid-phase peptide synthesis.

63 sequence, themselves obtained by solid-phase peptide synthesis.

64 fectively as melittin created by solid phase peptide synthesis.

65 analogs were synthesized through solid-phase peptide synthesis.

66 ly compatible with standard Fmoc solid-phase peptide synthesis.

67 solid phase synthesis procedures similar to peptide synthesis.

68 B and pPLB standards produced by solid-phase peptide synthesis.

69 ere generated through Fmoc-based solid phase peptide synthesis.

70 ragments, themselves prepared by solid phase peptide synthesis.

71 site-specifically using standard solid-phase peptide synthesis.

72 at this fragment, synthesized by solid-phase peptide synthesis, also forms fibrillar structures in wa

73 We used peptide synthesis and 2D NMR spectroscopy to assign all

74 by modification of strategies for linear aza-peptide synthesis and applied in the preparation of cycl

75 is synthesized using Fmoc-based solid-phase peptide synthesis and assembled using combined native ch

76 ave thus demonstrated that using solid phase peptide synthesis and chemical ligation it is feasible t

77 ystem to emphasize the enormous diversity in peptide synthesis and consequent complexity of the still

78 apeptide skeleton assembled via nonribosomal peptide synthesis and constrained through multiple cross

79 a 22-mer peptide, is prepared by solid-phase peptide synthesis and contains the F(n)Y at the desired

80 lated Gla domain of Factor IX by solid phase peptide synthesis and crystallized Factor IX-(1-47) in c

81 es were synthesized by solid-phase supported peptide synthesis and elongated with respective chelator

82 9-124) were prepared by stepwise solid-phase peptide synthesis and ligated to yield a peptide bond be

83 ed by a tandem combination using solid phase peptide synthesis and microwave-assisted reactions.

84 Although peptide synthesis and modification are well established,

85 Peptide synthesis and MS/MS confirmed the kynurenine ass

86 tion into peptides and proteins via existing peptide synthesis and protein expression methods, we bel

87 se release factors is essential for accurate peptide synthesis and ribosome recycling.

88 es were successfully utilized in solid-phase peptide synthesis and semi-synthesis of histone H4.

89 Although the cytoplasmic peptide synthesis and side-chain modifications are well

90 oth the influence of cation concentration on peptide synthesis and the effect of altering the uORF se

91 LLELI[13C615N1]R was prepared by solid-phase peptide synthesis and was used as an internal standard t

92 ptides using Boc-chemistry-based solid-phase peptide synthesis, and in three of the four cases the re

93 Determination of the swelling properties, peptide synthesis, and on-bead streptavidin-alkaline pho

94 onceptually innovative approach to amide and peptide synthesis, and one that might ultimately provide

95 could be recovered in up to 98% yield after peptide synthesis, and the recovered support was utilize

96 tides using Fmoc-chemistry-based solid-phase peptide synthesis, and the resulting peptides can be che

97 cation-pi binding energies using solid-phase peptide synthesis, and these analogues were modified by

98 study initiation and elongation in ribosomal peptide synthesis, and to screen for eubacterial-specifi

99 results show the power of optimized chemical peptide synthesis approaches for the efficient productio

100 Using popular terminology, what forms of peptide synthesis are appropriately termed "on-demand"?

101 and the implications for the origin of coded peptide synthesis are considered.

102 Several case studies in complex peptide synthesis are summarized here, each selected to

103 stly adopted in the solution and solid-phase peptide synthesis, are compatible to the adopted reactio

104 Translation, coded peptide synthesis, arguably exists at the heart of moder

105 , and Abeta(5-38(D23S)) by using solid phase peptide synthesis as internal standards for the CIEF imm

106 of this chemistry to reiterative solid-phase peptide synthesis as well as solid-phase fragment coupli

107 articularly convenient for hybrid alpha/beta-peptide synthesis, as demonstrated recently by our group

108 This quantitative in vitro peptide synthesis assay allowed us to analyze the abilit

109 e residues by using mutational analyses in a peptide synthesis assay with intact bacillamide syntheta

110 proline analogs require eIF5A for efficient peptide synthesis, azetidine-2-caboxylic acid, a more fl

111 These approaches include solid-phase peptide synthesis based on an adaption of native chemica

112 of genes previously implicated in interchain peptide synthesis but with unknown specific functions.

113 favor the lauroyl glycine synthesis over the peptide synthesis, but the immobilized protease has the

114 ally similar to the present-day nonribosomal peptide synthesis by multi-enzyme thiotemplate systems.

115 Endothelin-1, a peptide synthesised by vascular endothelial cells, is th

116 to climb the TR barrier in parallel with the peptide synthesis chemical step and that the nascent cha

117 s synthesized using conventional solid-phase peptide synthesis chemistry and a rhenium cyclization re

118 ze DDMs using solution phase and solid phase peptide synthesis chemistry.

119 st-generation cross-linker using solid-phase peptide synthesis chemistry.

120 acetyltransferase, and VibH, a non-ribosomal peptide synthesis condensation enzyme.

121 an be manipulated under standard solid-phase peptide synthesis conditions.

122 Peptide synthesis depended on sequence complementarity b

123 fostered in part by advances in large-scale peptide synthesis, development of peptides as therapeuti

124 acid substitutions have been constructed by peptide synthesis; each impairs binding to boxB.

125 ntacyclic regions that derive from ribosomal peptide synthesis followed by extensive posttranslationa

126 -OH (10), were synthesized using solid phase peptide synthesis followed by rhenium cyclization.

127 lding blocks are compatible with solid-phase peptide synthesis following the 9-fluorenylmethoxycarbon

128 ide carbonyl derivatives rely on solid-phase peptide synthesis for amide functionalization.

129 per, we provide the first direct evidence of peptide synthesis from the S-adenosylmethionine decarbox

130 ere involved in noncoded thioester-dependent peptide synthesis, functionally similar to the present d

131 ere involved in noncoded thioester-dependent peptide synthesis, functionally similar to the present-d

132 Solid-phase peptide synthesis has been an attractive method for synt

133 Solid-state peptide synthesis has been applied to link the acids to

134 Solid-phase peptide synthesis has been applied to the preparation of

135 When the peptide synthesis has been completed, activation of the

136 to defined biopolymers by automated DNA and peptide synthesis has fundamentally altered biological r

137 Multiple sequence analysis and peptide synthesis have identified a core set of residues

138 Molecular modeling associated with peptide synthesis improved bactericidal efficacy in addi

139 Combinatorial peptide synthesis in combination with affinity selection

140 of 100 amino acid residues using solid phase peptide synthesis in combination with native chemical li

141 as the detection method for the solid-phase peptide synthesis in combinatorial chemistry.

142 Peptide synthesis in eukaryotes terminates when eukaryot

143 ying computational simulation techniques and peptide synthesis in liquid phase.

144 IL-23 drive substance P gene expression and peptide synthesis in murine splenic T cells and macropha

145 The protecting group can be applied to peptide synthesis in solution as well as on a solid phas

146 al study of different possible mechanisms of peptide synthesis in the ribosome has been carried out u

147 n of proline, whose unique side-chain stalls peptide synthesis in vitro, also slows the ribosome in v

148 g blocks, suitably protected for solid-phase peptide synthesis, in 2-3 steps starting from inexpensiv

149 tion of microwave irradiation to solid-phase peptide synthesis increases product purity and reduces r

150 that PGRP-LC is important for antibacterial peptide synthesis induced by Escherichia coli both in vi

151 odules were incorporated through solid-phase peptide synthesis into a 13-residue helical oligoproline

152 Nonribosomal peptide synthesis involves the interplay between covalen

153 Peptide synthesis is a truly interdisciplinary tool, fam

154 The central chemical step of peptide synthesis is amide bond formation, which is typi

155 riately protected for Fmoc-based solid-phase peptide synthesis is described.

156 embodying a credible early history for coded peptide synthesis is readily constructed based on these

157 ded from a saturated sugar-transport system; peptide synthesis is reduced under high-light, high CO(2

158 herichia coli and most other microorganisms, peptide synthesis is started at methionine start codons

159 luding the Fmoc/tBu strategy for solid phase peptide synthesis, is stable under mild acidic condition

160 vel hybrid unnatural amino acids, during the peptide synthesis itself.

161 oncise assessment of the state-of-the-art in peptide synthesis look like?

162 s employs protocols derived from solid-phase peptide synthesis, making the methodology straightforwar

163 A solid-phase peptide synthesis methodology that allows for the rapid

164 = 2-6) have been synthesized by solid-phase peptide synthesis methods and characterized by 1H NMR sp

165 Using solid-phase peptide synthesis methods, two units of the mannosyl der

166 obutylglycine), were prepared by solid-phase peptide synthesis methods.

167 ocell with an RNA genome, ribozyme-catalysed peptide synthesis might have been sufficient to initiate

168 nalogs at several steps along the pathway to peptide synthesis, much of the specificity resides at th

169 Ribosomes that stall before completing peptide synthesis must be recycled and returned to the c

170 e of the main techniques, namely solid phase peptide synthesis, native chemical ligation, Staudinger

171 heterogeneous reaction medium in solid-phase peptide synthesis necessitates the use of large equivale

172 al protein did not affect factor-independent peptide synthesis; nor did lethal mutations of nucleotid

173 e demonstrated functions in the nonribosomal peptide synthesis (NRPS)/polyketide (PK) synthesis or tr

174 le of mRNA translation, the process by which peptide synthesis occurs according to the genetic code t

175 ets" are synthesized by standard solid-phase peptide synthesis of a linear precursor followed by solu

176 ation of the products during the solid-phase peptide synthesis of glycine, alanine, and valine mediat

177 applied in the manual Fmoc-based solid-phase peptide synthesis of Leu-enkephalin and in microwave-ass

178 how here the first full stepwise solid phase peptide synthesis of mambalgin-1 and confirm the biologi

179 Solid-phase peptide synthesis of several flavopeptin species and der

180 rtain thioesterases involved in nonribosomal peptide synthesis of siderophores and antibiotics.

181 an be carried out as part of the solid-phase peptide synthesis, or it can be undertaken in the soluti

182 w and innovative applications in organic and peptide synthesis, polymer chemistry, material sciences,

183 ive heating occasionally has been applied to peptide synthesis, precise microwave irradiation to heat

184 esidue into peptides by standard solid-phase peptide synthesis procedures.

185 ked glycopeptide was extended using standard peptide synthesis procedures.

186 The microwave-assisted parallel peptide synthesis protocol has been used to generate a l

187 However, standard peptide synthesis protocols are not efficient enough to

188 lysine derivative can be used in solid-phase peptide synthesis, providing access to proteins that con

189 e conjugates were synthesized by solid-phase peptide synthesis, purified by reversed-phase high-perfo

190 s application is limited by the high cost of peptide synthesis, rapid proteolysis, and poor efficacy

191 d building blocks, followed by a solid-phase peptide synthesis sequence, featuring two resin-bound ma

192 e usefulness of this strategy in solid-phase peptide synthesis, several bioactive peptides have been

193 zing coupling reagents typically employed in peptide synthesis, several different deoxyguanosine nucl

194 nes are not recommended as intermediates for peptide synthesis, since model studies showed that lacto

195 ne [Trt-Cys(Xan)-OH] has been introduced for peptide synthesis, specifically for application to a new

196 ues, which are incompatible with solid-phase peptide synthesis (SPPS) due to the intrinsic acid labil

197 ha-Azido acids have been used in solid phase peptide synthesis (SPPS) for almost 20 years.

198 canoic acid) required to support solid phase peptide synthesis (SPPS) for structure-activity studies

199 Solid-phase peptide synthesis (SPPS) is a widely used technique in b

200 synthetic approach relies on the solid-phase peptide synthesis (SPPS) of N-terminal thioesters (inclu

201 They are prepared by solid-phase peptide synthesis (SPPS) or biosynthetically by protein

202 r-Ile-Asn-Gly-OH), following the solid-phase peptide synthesis (SPPS) protocol and Amyloid beta (39-4

203 The use of solid-phase peptide synthesis (SPPS) to prepare four such arrays, co

204 Solid-phase peptide synthesis (SPPS) using tert-butyloxycarbonyl (Bo

205 are directly incorporated after solid-phase peptide synthesis (SPPS) via on-resin derivatization of

206 elator to be incorporated during solid-phase peptide synthesis (SPPS) with total site specificity.

207 building blocks during automated solid phase peptide synthesis (SPPS), followed by orthogonal deprote

208 r thiopeptide cores prepared via solid-phase peptide synthesis (SPPS), giving an efficient and modula

209 riately protected for Fmoc-based solid-phase peptide synthesis (SPPS), is described.

210 e 20, suitable for Fmoc-strategy solid-phase peptide synthesis (SPPS), was achieved in four steps fro

211 ag, available via semi-automated solid-phase peptide synthesis (SPPS), while equipping the antibody w

212 ribe a simple and efficient Fmoc solid-phase peptide synthesis (SPPS)-based method for synthesizing d

213 enient use as building blocks in solid phase peptide synthesis (SPPS).

214 )-fluorenylmethoxycarbonyl-based solid-phase peptide synthesis (SPPS).

215 cal ligation (NCL) reaction with solid phase peptide synthesis (SPPS).

216 compatibility with standard Fmoc solid-phase peptide synthesis (SPPS).

217 amino acids at variable positions during the peptide synthesis step.

218 s from the crude preparations of solid-phase peptide synthesis step.

219 te amino acids are amenable to standard Fmoc peptide synthesis strategy, and the resulting compounds

220 s been developed on the basis of solid-phase peptide synthesis techniques and is accomplished through

221 orated into peptides by standard solid-phase peptide synthesis techniques.

222 PAGE) with peptides produced via solid-phase peptide synthesis that correspond to the TM domain of FG

223 ent study describes innovations in poly-beta-peptide synthesis that enable the preparation of diverse

224 ions, carries out a function in nonribosomal peptide synthesis that is analogous to the function of t

225 that might ultimately provide for efficient peptide synthesis that is fully reliant on enantioselect

226 To investigate one kind of template-like peptide synthesis that might emerge from an RNA world, w

227 After AQUA peptide synthesis, the development, optimization and app

228 After peptide synthesis, the Hyp protecting group is orthogona

229 After peptide synthesis, the resin bound peptide was cleaved u

230 Standard solid-phase peptide synthesis then resulted in the desired amide-lin

231 mino acids are not accommodated in ribosomal peptide synthesis, these results suggest that the same p

232 ide synthetases (NRPSs) carry out instructed peptide synthesis through a series of directed intermodu

233 ng materials and was employed in solid-phase peptide synthesis to afford the desired cyclic peptide s

234 e building blocks for Fmoc-based solid-phase peptide synthesis to allow convenient incorporation of t

235 this reason, some may perceive even complex peptide synthesis to be a "solved problem", while others

236 lactam linkage were prepared by solid phase peptide synthesis to explore possible biologically activ

237 cal methods, taking advantage of solid-phase peptide synthesis to incorporate approximately isosteric

238 ides are synthesized by standard solid-phase peptide synthesis to incorporate Fmoc-hydroxyproline (4R

239 emolysin (alphaHL) pore by using solid-phase peptide synthesis to make the central segment of the pol

240 (N-(9-fluorenyl)methoxycarbonyl) solid-phase peptide synthesis to prepare alpha-amino-n-butyric acid

241 1-carboxylic acid (1), which will be used in peptide synthesis to prepare glycopeptides containing ca

242 aced with diaminopimelate during solid-phase peptide synthesis to produce several analogues.

243 According to the RNA world hypothesis, coded peptide synthesis (translation) must have been first cat

244 The method is based on solid-phase peptide synthesis using 2-chlorotrityl resin as the soli

245 stone N termini were prepared by solid phase peptide synthesis using an acid labile Boc/HF assembly s

246 ing an N-Me group during regular solid-phase peptide synthesis using Boc protection.

247 n proteolysis was synthesized by solid-phase peptide synthesis using known mixtures of natural abunda

248 Peptide synthesis using soluble, yet isolable, supports

249 conjugated to bombesin(7-14) by solid-phase peptide synthesis using standard Fmoc chemistry.

250 challenging in the past because solid-phase peptide synthesis usually starts from the C-terminus, wh

251 eselected synthetic sequence via solid phase peptide synthesis was designed to produce 2,3-diaminopro

252 Solid-phase peptide synthesis was employed to elaborate the requisit

253 oc-protected DOTAla suitable for solid phase peptide synthesis was synthesized and integrated into po

254 Peptide synthesis was used to create a fully functional

255 Peptide synthesis was used to verify the determined sequ

256 Here, using chemical peptide synthesis, we further confirmed the importance o

257 Using the SPOTs technique of peptide synthesis, we identified the sequences in Mena t

258 alogues synthesized by Fmoc/t-Bu solid phase peptide synthesis were used to analyze their enzymatic d

259 54-375 of R2 was generated using solid-phase peptide synthesis where 354, a serine in the wild-type (

260 refers to a hypothetical era prior to coded peptide synthesis, where RNA was the major structural, g

261 ith HOAt are excellent coupling reagents for peptide synthesis which are generally superior to their

262 5-9) and blocking amino acids in peptide synthesis with 2-nitrobenzyl compounds.

263 nthesized in Boc-protected form suitable for peptide synthesis with an overall yield of 20% in 10 ste

264 ished through the combination of solid-phase peptide synthesis with detailed liquid chromatography-ma

265 ese peptides were prepared using solid-phase peptide synthesis with Fmoc alpha-amino protection.

266 iew of the advances in microwave heating for peptide synthesis, with a focus on systematic studies an

267 bably through the processing of a regulatory peptide synthesised within the developing cyst.