ライフサイエンスコーパス: peptide synthetase

1 f the termination module of the nonribosomal peptide synthetase.

2 ree-subunit (TycABC), 10-module nonribosomal peptide synthetase.

3 ntermediates on a multimodular, nonribosomal peptide synthetase.

4 ame (HTS1) encoding a multifunctional cyclic peptide synthetase.

5 (CepA, CepB, and CepC) modular nonribosomal peptide synthetase.

6 ety of ansatrienin from shikimic acid, and a peptide synthetase.

7 hesized on ribosomes and not by nonribosomal peptide synthetases.

8 ynthesized wholly or in part by nonribosomal peptide synthetases.

9 chain activities within dimeric nonribosomal peptide synthetases.

10 genes, orbI and orbJ, encoding nonribosomal peptide synthetases.

11 etases, firefly luciferase, and nonribosomal peptide synthetases.

12 assembled by hybrid polyketide/nonribosomal peptide synthetases.

13 nylation domains of the modular nonribosomal peptide synthetases.

14 between monomers and dimers in nonribosomal peptide synthetases.

15 me A ligases, luciferases, and non-ribosomal peptide synthetases.

16 ld enable engineering of novel non-ribosomal peptide synthetases.

17 been less well characterized in nonribosomal peptide synthetases.

18 ation and thiolation domains of nonribosomal peptide synthetases.

19 ally similar to the present day nonribosomal peptide synthetases.

20 nsation domain found in certain nonribosomal peptide synthetases.

21 oach has been unprecedented for nonribosomal peptide synthetases.

22 s, in contrast to the monomeric nonribosomal peptide synthetases.

23 presents a novel substrate for non-ribosomal peptide synthetases.

24 ed by two large multifunctional enzymes, the peptide synthetases 1 and 2.

25 ere we report an unprecedented non-ribosomal peptide synthetase activity that both assembles a serine

26 airwise Southern hybridizations identified a peptide synthetase acyladenylation module conserved amon

27 the substrate specificities of nonribosomal peptide synthetase adenylation (A) domains from DNA sequ

28 ate specificity prediction for non-ribosomal peptide synthetase adenylation domains based on the new

29 s a large rotation compared to structures of peptide synthetase adenylation domains.

30 trate specificities of related non-ribosomal peptide synthetase adenylation enzymes.

31 e screened syn-BNPs inspired by nonribosomal peptide synthetases against microbial pathogens, and dis

32 imodular polyketide synthase, a nonribosomal peptide synthetase, along with enzymes responsible for t

33 f a gene, HTS1, that encodes a 570-kD cyclic peptide synthetase and is lacking in Tox2- isolates.

34 The hybrid nonribosomal peptide synthetase and polyketide synthase biosynthetic

35 DszC, with DszC containing both nonribosomal peptide synthetase and polyketide synthase modules.

36 exploring interactions between nonribosomal peptide synthetase and polyketide synthase modules.

37 e multidomain enzymes, known as nonribosomal peptide synthetases and polyketide synthases (NRPSs and

38 in part by enzymes resembling non-ribosomal peptide synthetases and that the ABC transporter ExiT is

39 at includes firefly luciferase, nonribosomal peptide synthetases, and acyl- and aryl-CoA synthetases/

40 own formally classified family, nonribosomal peptide synthetases, and several hybrid clusters.

41 is methodology to other dimeric nonribosomal peptide synthetases, and to the related fatty acid and p

42 Non-ribosomal peptide synthetases are giant enzymes composed of module

43 hus, full reconstitution of the nonribosomal peptide synthetase assembly line by purified protein com

44 ay for this polyketide synthase/nonribosomal peptide synthetase assembly line.

45 successfully expressed a 67-kb nonribosomal peptide synthetase biosynthetic gene cluster from the ma

46 modular polyketide synthase and nonribosomal peptide synthetase biosynthetic pathways are a rich sour

47 he organization of the deduced TxtA and TxtB peptide synthetase catalytic domains is consistent with

48 not previously associated with nonribosomal peptide synthetase chemistry.

49 We show that nonribosomal peptide synthetases ClbN and ClbB assemble and process a

50 biosynthesis of L-PAPA and the nonribosomal peptide synthetase CmlP.

51 We identified two peptide synthetase coding regions interrupted by a 10- t

52 is assembled by a four-subunit nonribosomal peptide synthetase complex, VibE, VibB, VibH, and VibF,

53 The nonribosomal peptide synthetase consisted of a priming module, six ex

54 tins are synthesized through a non-ribosomal peptide synthetase containing a terminal NAD(P)H-depende

55 e carboxy terminus carrying the nonribosomal peptide synthetase cores, as well as the site-directed m

56 encoding proteins homologous to nonribosomal peptide synthetases, cytochrome P450-related oxidases, f

57 The pathway builds on a nonribosomal peptide synthetase de-rived di-tyrosine piperazine inter

58 is a polyketide synthase (PKS)-non-ribosomal peptide synthetase-derived natural product with potent a

59 he largest continuous bacterial nonribosomal peptide synthetase discovered so far is described.

60 ary metabolites synthesized by non-ribosomal peptide synthetases display diverse and complex topologi

61 od are demonstrated on a 37 kDa nonribosomal peptide synthetase domain subject to spectral overlap.

62 genes encoding for a six-module nonribosomal peptide synthetase EcdA, an acyl-AMP ligase EcdI, and ox

63 e (2,3-DHB) and l-serine by the nonribosomal peptide synthetases EntB and EntF.

64 anine adenylation domain of the nonribosomal peptide synthetase enzyme gramicidin S synthetase A (Grs

65 odification of the product of a nonribosomal peptide synthetase enzyme, whose consequences are predic

66 heterocyclization ability in a nonribosomal peptide synthetase enzyme.

67 set of polyketide synthases and nonribosomal peptide synthetases evolve by concerted evolution, which

68 e whether CepA, a three-module 3,158-residue peptide synthetase expected to assemble the first three

69 se (PKS) subunits (fkbB, fkbC and fkbA), the peptide synthetase (fkbP), the 31-O-methyltransferase (f

70 VbsS, a nonribosomal peptide synthetase free-standing module, then activates

71 identified, the non-canonical non-ribosomal peptide synthetase Fub8.

72 tified a 59-kb cryptic inactive nonribosomal peptide synthetase gene cluster proposed to be responsib

73 in, a major product of the gli non-ribosomal peptide synthetase gene cluster, is strongly associated

74 dicted lipopeptide encoded by a nonribosomal peptide synthetase gene cluster.

75 a widely distributed family of nonribosomal peptide synthetase gene clusters in gut bacteria.

76 We have cloned a similar peptide synthetase gene from Neotyphodium lolii and inac

77 A peptide synthetase gene hypothesized to be important for

78 Each cosmid hybridized with one or more peptide synthetase gene probes and two also hybridized w

79 pregulate transcription of the non-ribosomal peptide synthetase gene required for nidulanin A biosynt

80 er of nine genes (including one nonribosomal peptide synthetase gene, ataP) that is required for acet

81 The complete nucleotide sequences of two peptide synthetase genes (txtAB) were determined from S.

82 ed portions of the acyladenylation module of peptide synthetase genes from genomic DNA of representat

83 We studied the non-ribosomal peptide synthetase genes involved in A21978C biosynthesi

84 Individual inactivation of the non-ribosomal peptide synthetase genes, xcnA and xcnK, and polyketide

85 gliP, the gene that encodes the nonribosomal peptide synthetase GliP.

86 sembly by polyketide synthases, nonribosomal peptide synthetases, glycosyltransferases and other enzy

87 A gene encoding a putative peptide synthetase has been cloned and partially sequenc

88 lar weight protein 2 (HMWP2), a nonribosomal peptide synthetase homologue, and YbtE in the initiation

89 Expression of all or parts of the peptide synthetase in Escherichia coli would facilitate

90 ed using the recently described nonribosomal peptide synthetase independent siderophore (NIS) biosynt

91 for both siderophores involve a nonribosomal peptide synthetase independent siderophore (NIS) synthet

92 acyl carrier protein-dependent, nonribosomal peptide synthetase-independent biosynthetic system.

93 a three-dimensional model of a nonribosomal peptide synthetase-independent siderophore (NIS) synthet

94 A non-ribosomal peptide synthetase-independent siderophore biosynthetic

95 AcsD and other members of the non-ribosomal peptide synthetase-independent siderophore superfamily m

96 a member of the superfamily of nonribosomal peptide synthetase-independent siderophore synthetases (

97 PMI0229-0239 encodes a non-ribosomal peptide synthetase-independent siderophore system for pr

98 respectively, which are highly homologous to peptide synthetases, indicating that exochelin biosynthe

99 esis of aminoacyl-thioesters by nonribosomal peptide synthetases is relaxed, whereas the amino acid s

100 NPS6, encoding a nonribosomal peptide synthetase, is a virulence determinant in the ma

101 ng hybrid PKS-PKS and PKS-NRPS (nonribosomal peptide synthetase) junctions and suggests fundamental g

102 t load carrier domains found in nonribosomal peptide synthetases, large molecule mass spectrometry is

103 quent enzymatic assays with the nonribosomal peptide synthetase-like AsbC, AsbD, and AsbE polypeptide

104 we discovered an unprecedented nonribosomal peptide synthetase-like-pteridine synthase hybrid biosyn

105 e is assembled from tyrosine by nonribosomal peptide synthetase logic.

106 ergopeptines requires the activities of two peptide synthetases, LPS1 and LPS2.

107 ng family of enzymes, including nonribosomal peptide synthetases, luciferase, and aryl- and acyl-CoA

108 er (siderophore) and product of nonribosomal peptide synthetase machinery, to the C-terminal serine r

109 ts loading module, which uses a nonribosomal peptide synthetase mechanism, and its initial elongation

110 dule (KS-AT-MT(2)-KR-ACP) and a nonribosomal peptide synthetase module (Cy(3)-MT(3)-PCP(3)-TE).

111 ical analysis of the N-terminal nonribosomal peptide synthetase module from the Thalassospira TtcA me

112 re, TxtB was identified as the non-ribosomal peptide synthetase module specific for 4-nitrotryptophan

113 Here we show that DdaD, a nonribosomal peptide synthetase module, activates and sequesters N(be

114 through the actions of CmaA, a non-ribosomal peptide synthetase module, and CmaE, an unusual acyltran

115 mposed of a loading module, one nonribosomal peptide synthetase module, eight polyketide synthase mod

116 produced by the action of two non-ribosomal peptide synthetase modules (TxtA and TxtB) and a complem

117 pected polyketide synthase and non-ribosomal peptide synthetase modules and tailoring genes.

118 e predicted protein structure consists of 18 peptide synthetase modules with additional modifying dom

119 ructures of two different holo-non-ribosomal peptide synthetase modules, each revealing a distinct st

120 odifies polyketide synthase and nonribosomal peptide synthetase modules.

121 VbsS, which is similar to many non-ribosomal peptide synthetase multienzymes, has a central role.

122 mechanistic studies of the two nonribosomal peptide synthetases NocA and NocB that lie at the heart

123 zymatic assembly line with both nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS)

124 They are synthesized on modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS)

125 ach to clone a thiazole-forming nonribosomal peptide synthetase (NRPS) as a probe, we localized a 172

126 tapeptide on the thio-templated nonribosomal peptide synthetase (NRPS) assembly line protein PacH.

127 e or intermediates bound to the nonribosomal peptide synthetase (NRPS) assembly line.

128 canonical epimerase domains of nonribosomal peptide synthetase (NRPS) assembly lines found in the Yb

129 FUM14 was predicted to encode a nonribosomal peptide synthetase (NRPS) containing two domains, peptid

130 ssociated nature of the encoded nonribosomal peptide synthetase (NRPS) domains and modules.

131 fungal anthranilate-activating nonribosomal peptide synthetase (NRPS) domains through bioinformatics

132 All three are homologous to nonribosomal peptide synthetase (NRPS) domains: VibE is a 2,3-dihydro

133 ed by polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) enzymes EpoA-F.

134 The activity of several nonribosomal peptide synthetase (NRPS) enzymes is used to combine the

135 yclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes.

136 rio harveyi BAA-1116 contains a nonribosomal peptide synthetase (NRPS) gene cluster (aebA-F) resembli

137 ajor product of the cryptic has nonribosomal peptide synthetase (NRPS) gene cluster in the human path

138 e applied to the known PKS and non-ribosomal peptide synthetase (NRPS) gene clusters in the Bacillus

139 self-resistance gene (vph) and non-ribosomal peptide synthetase (NRPS) gene probes amplified from S.

140 by a polyketide synthase (PKS)-nonribosomal peptide synthetase (NRPS) hybrid involving proteins EpoA

141 Genetic engineering of the nonribosomal peptide synthetase (NRPS) in the daptomycin biosynthetic

142 main organization of the ncpA-B nonribosomal peptide synthetase (NRPS) is co-linear in arrangement wi

143 tin (Ybt) synthetase, a 230 kDa nonribosomal peptide synthetase (NRPS) making the N-terminus of the Y

144 guillarum, is synthesized via a nonribosomal peptide synthetase (NRPS) mechanism.

145 CrpD-M2 is a unique non-ribosomal peptide synthetase (NRPS) module comprised of condensati

146 acyltransferase, a stand-alone nonribosomal peptide synthetase (NRPS) module, and four flavin-depend

147 ent-state kinetic analysis of a nonribosomal peptide synthetase (NRPS) module.

148 ), including highly dissociated nonribosomal peptide synthetase (NRPS) modules and a variety of tailo

149 cluster encodes a series of six nonribosomal peptide synthetase (NRPS) modules distributed over three

150 lized metabolites derived from non-ribosomal peptide synthetase (NRPS) or polyketide synthase (PKS).

151 a polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) pathways.

152 Many bacteria use nonribosomal peptide synthetase (NRPS) proteins to produce peptide an

153 cymA, which encodes a 7-module nonribosomal peptide synthetase (NRPS) responsible for assembly of th

154 rmined the function of a novel non-ribosomal peptide synthetase (NRPS) system carried by a streptococ

155 ybrid polyketide synthase (PKS)/nonribosomal peptide synthetase (NRPS) systems in streptomycetes.

156 The EpoB protein is a non-ribosomal peptide synthetase (NRPS) that catalyzes formation of th

157 PKS is fused to a single module nonribosomal peptide synthetase (NRPS) to synthesize polyketides conj

158 The six-domain, 270 kDa nonribosomal peptide synthetase (NRPS) VibF, a component of vibriobac

159 from the multidomain tyrocidine nonribosomal peptide synthetase (NRPS) was recently shown to catalyze

160 of polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), and shikimate pathway compone

161 scent product of the LNM hybrid nonribosomal peptide synthetase (NRPS)-acyltransferase (AT)-less type

162 ecursor of chloramphenicol in a nonribosomal peptide synthetase (NRPS)-based pathway to yield the nit

163 protein (EntE, B, F) six-module nonribosomal peptide synthetase (NRPS).

164 inates from a fungal trimodular nonribosomal peptide synthetase (NRPS).

165 of the MBT backbone by a hybrid nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) mega

166 Non-ribosomal peptide synthetases (NRPS) and polyketide synthases (PKS

167 Type II nonribosomal peptide synthetases (NRPS) generate exotic amino acid de

168 The initiation module of non-ribosomal peptide synthetases (NRPS) selects and activates the fir

169 ing systems can be assembled by nonribosomal peptide synthetases (NRPS) using the conformationally re

170 es, the adenylation domains of non-ribosomal peptide synthetases (NRPS), and firefly luciferase, perf

171 Nonribosomal peptide synthetases (NRPS), fatty acid synthases (FAS),

172 Nonribosomal peptide synthetases (NRPS), fatty acid synthases (FAS),

173 Nonribosomal peptide synthetases (NRPS), polyketide synthases (PKS),

174 five Aspergillus species, the non-ribosomal peptide synthetases (NRPS).

175 domain-domain communication in nonribosomal peptide synthetases (NRPSs) and lay the groundwork for t

176 ackbone of PVD is assembled by non-ribosomal peptide synthetases (NRPSs) and modified by accessory en

177 l properties are synthesized by nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PK

178 Nonribosomal peptide synthetases (NRPSs) and polyketide synthases (PK

179 Nonribosomal peptide synthetases (NRPSs) and polyketide synthases are

180 Nonribosomal peptide synthetases (NRPSs) are a family of multidomain,

181 Nonribosomal peptide synthetases (NRPSs) are microbial enzymes that p

182 The nonribosomal peptide synthetases (NRPSs) are one of the most promisin

183 Polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) are two related families of

184 Nonribosomal peptide synthetases (NRPSs) assemble a large group of st

185 Ribosomes and nonribosomal peptide synthetases (NRPSs) carry out instructed peptide

186 Nonribosomal peptide synthetases (NRPSs) catalyze the biosynthesis of

187 thioesterase (TE) domains from nonribosomal peptide synthetases (NRPSs) catalyze the final step in t

188 polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) comprise giant multidomain e

189 Nonribosomal peptide synthetases (NRPSs) make many natural products o

190 s on the carrier domains of the nonribosomal peptide synthetases (NRPSs) or polyketide synthases (PKS

191 Nonribosomal peptide synthetases (NRPSs) produce a wide variety of pe

192 teria, polyketide synthases and nonribosomal peptide synthetases (NRPSs) produce complex lipidic meta

193 olyketide synthases (PKSs) and non-ribosomal peptide synthetases (NRPSs) that contain repeated, coord

194 arison with the large number of nonribosomal peptide synthetases (NRPSs) that release their peptide p

195 Nonribosomal peptide synthetases (NRPSs) use phosphopantetheine (pPan

196 ng polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and mixed PKS/NRPS systems,

197 cluster predicted that the two nonribosomal peptide synthetases (NRPSs), BlmIV and BlmIII, are respo

198 f enzyme-bound intermediates in nonribosomal peptide synthetases (NRPSs), mass spectrometry is used t

199 cin A gene cluster encodes two non-ribosomal peptide synthetases (NRPSs), NocA and NocB, predicted to

200 nes encoding fatty acid ligase, nonribosomal peptide synthetases (NRPSs), regulators, transporters, a

201 d from amino acid precursors by nonribosomal peptide synthetases (NRPSs), which are organized into mo

202 ts are produced by multidomain non-ribosomal peptide synthetases (NRPSs).

203 e also shown to originate from non-ribosomal peptide synthetases (NRPSs).

204 rge, multidomain enzymes called nonribosomal peptide synthetases (NRPSs).

205 nthesis of natural products by non-ribosomal peptide synthetases (NRPSs).

206 creted proteins, a multisubunit nonribosomal peptide synthetase operon, and genes encoding two member

207 irst direct characterization of nonribosomal peptide synthetase oxidase domain activity and will lead

208 bacterin is biosynthesized by a nonribosomal peptide synthetase pathway.

209 ty of future engineering within nonribosomal peptide synthetase pathways using oxidase domains.

210 two module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) that generates cyclo-aceto

211 two module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) that makes and releases cy

212 ngle-module polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS), although two separate hex

213 es a hybrid polyketide synthase nonribosomal peptide synthetase (PKS-NRPS), which resembles iterative

214 lyketide siderophores involves non-ribosomal peptide synthetase, polyketide synthase and non-ribosoma

215 tors biosynthesized by a hybrid nonribosomal peptide synthetase-polyketide synthase (NRPS-PKS) system

216 of the nos synthetase, a mixed non-ribosomal peptide synthetase-polyketide synthase, is co-linear in

217 sized by the action of a hybrid nonribosomal peptide synthetase/polyketide synthase in the myxobacter

218 y backbone assembly by a hybrid nonribosomal peptide synthetase/polyketide synthase.

219 osynthetic loci encoding hybrid nonribosomal peptide synthetase/polyketide synthases consistent with

220 II thioesterase from the hybrid nonribosomal peptide synthetases/polyketide synthase rifamycin biosyn

221 uced genes showed homology with nonribosomal peptide synthetases/polyketide synthases.

222 Peptide synthetases produce a wide variety of peptide se

223 inal thioesterase domain of the nonribosomal peptide synthetase producing the lipopetide surfactin (S

224 500 microM sample of the 33 kDa nonribosomal peptide synthetase protein EntB.

225 le motifs characteristic of the nonribosomal peptide synthetase protein family were identified in eac

226 end of the sequence reported here encodes a peptide synthetase required for the activation and incor

227 The excised TE domain from the nonribosomal peptide synthetase responsible for the production of the

228 n domains of PchE and PchF, the nonribosomal peptide synthetases responsible for the biosynthesis of

229 Nonribosomal peptide synthetases responsible for the production of ma

230 nd the following spacer is typical of fungal peptide synthetases, showing good conservation of the si

231 evealed the central role of the nonribosomal peptide synthetase Sln9 in constructing and installing t

232 covalently modified serines in nonribosomal peptide synthetases, streamlined methodologies described

233 All six domains of this nonribosomal peptide synthetase subunit, three phosphopantetheinylata

234 d by a four-subunit, ten-domain nonribosomal peptide synthetase system, VibE, VibB, VibH, and VibF, u

235 nstalled into the scaffold by a nonribosomal peptide synthetase that also performs the subsequent mac

236 ) domain of the Yersinia pestis nonribosomal peptide synthetase that biosynthesizes the siderophore y

237 The non-ribosomal peptide synthetase that synthesizes HC-toxin has only on

238 ins of polyketide synthases and nonribosomal peptide synthetases to macrocycles via covalent enzyme b

239 zed from a classically derived non-ribosomal peptide synthetase tripeptide (from delta-(L-alpha-amino

240 ssion of nrps1 which encodes a non-ribosomal peptide synthetase was elevated in the ompR and envZ str

241 ogous to adenylation domains of nonribosomal peptide synthetases, was identified as specific for the

242 mately 450 residue E domains of nonribosomal peptide synthetases were constructed, and the PheATE der

243 ynthesis gene clusters encoding nonribosomal peptide synthetases were identified, emphasizing the pos

244 syringomycin are synthesized by nonribosomal peptide synthetases which are encoded by the syringomyci

245 sual hybrid polyketide synthase-nonribosomal peptide synthetase, which resembles iterative polyketide

246 tural basis for catalysis with non-ribosomal peptide synthetases will facilitate bioengineering to cr

247 We identify a nonribosomal peptide synthetase with an unusual domain architecture a

248 on of polyketide synthases and non-ribosomal peptide synthetases with unusual domain structures, incl