ライフサイエンスコーパス: peptidyl transferase

1 folding of the particles and inactivation of peptidyl transferase.

2 NA selection and binding, translocation, and peptidyl transferase.

3 s the conserved 2555 loop of 23S rRNA at the peptidyl transferase A site and suggests that peptide bo

4 directly or indirectly, helps to create the peptidyl transferase A site.

5 s of 23S rRNA likely provide elements of the peptidyl transferase active center that bind the reactan

6 of the bacterial 50S subunit, can reach the peptidyl transferase active site and contribute to its f

7 onserved 23S rRNA nucleotides located in the peptidyl transferase active site for transpeptidation an

8 venient, and sensitive method for monitoring peptidyl transferase activity (SPARK).

9 results in decreased 60S ribosome associated peptidyl transferase activity and inhibition of total pr

10 bunits with mutated A2451 showed significant peptidyl transferase activity in several independent ass

11 mal function, including the presumed site of peptidyl transferase activity in the 23S rRNA.

12 Though peptidyl transferase activity in the absence of protein

13 efects in E. coli and in decreased levels of peptidyl transferase activity in vitro.

14 t indicates that His343 is not essential for peptidyl transferase activity in yeast mitochondria.

15 In addition, the peptidyl transferase activity of 70 S ribosomes containi

16 fragment and cause a modest decrease in the peptidyl transferase activity of reconstituted ribosomes

17 tion sytem has been used to characterize the peptidyl transferase activity of site-directed mutations

18 titution results in dramatic decrease of the peptidyl transferase activity of the assembled subunits.

19 However, the antibiotic failed to inhibit peptidyl transferase activity of the H. halobium ribosom

20 ile pre-steady-state kinetic analysis of the peptidyl transferase activity of the mutant ribosomes re

21 l ortholog EFP, is proposed to stimulate the peptidyl transferase activity of the ribosome and facili

22 , A2602 (Escherichia coli numbering), on the peptidyl transferase activity of the ribosome.

23 Peptidyl transferase activity of Thermus aquaticus ribos

24 lity of ribosome-bound tryptophan to inhibit peptidyl transferase activity rather than by reducing th

25 In neither case was the loss of binding or peptidyl transferase activity suppressed by mutations in

26 ing of the wild-type (CCA) tRNA fragment and peptidyl transferase activity using the wild-type tRNA f

27 Finally, 60S ribosome-associated peptidyl transferase activity, a key enzyme for peptide

28 a decrease in growth rate, an impairment in peptidyl transferase activity, and a sharp decline in th

29 air has significant deleterious effects upon peptidyl transferase activity, but because G*U mutation

30 ribosomal P-site, showed drastically reduced peptidyl transferase activity, whereas clindamycin resis

31 s been implicated in a direct involvement in peptidyl transferase activity.

32 rase center, where bound tryptophan inhibits peptidyl transferase activity.

33 ribosomal subunit association as well as its peptidyl-transferase activity) caused accumulation of mR

34 within 18 A from the active site, revealing peptidyl transferase as an RNA enzyme.

35 scribed rRNAs exhibited high activity in the peptidyl transferase assay and in a poly(U)-dependent ce

36 This simplified peptidyl transferase assay follows a rapid equilibrium r

37 that of assisting the assembly of ribosomal peptidyl transferase by correctly positioning functional

38 inding, including significant changes in the peptidyl-transferase catalytic site.

39 idyl-tRNA analog to form a peptide bond in a peptidyl transferase-catalyzed reaction.

40 RNAs containing deleterious mutations in the peptidyl transferase center (25S NRD).

41 beling, and mutational analyses revealed the peptidyl transferase center (PTC) as the focal point of

42 its peptide-bond formation in the ribosome's peptidyl transferase center (PTC) during its own transla

43 The effect of AAP and Arg on ribosome peptidyl transferase center (PTC) function was analyzed

44 ribosomal exit tunnel and the A-site of the peptidyl transferase center (PTC) in halting translation

45 the CCA-end of the A-site tRNA away from the peptidyl transferase center (PTC) is functionally signif

46 Here we demonstrate that the ribosomal peptidyl transferase center (PTC) is supported by a fram

47 x RNA helicase specifically activated by the peptidyl transferase center (PTC) of 23S rRNA.

48 Peptide bond formation is catalyzed at the peptidyl transferase center (PTC) of the large ribosomal

49 protein synthesis inhibitors that target the peptidyl transferase center (PTC) on the large subunit o

50 nding of neither nucleotide (ATP or ADP) nor peptidyl transferase center (PTC) RNA, the presumed phys

51 ribosomal RNA (rRNA) helix 89 of the nascent peptidyl transferase center (PTC) through Nsa2.

52 tion axes for both subunits pass through the peptidyl transferase center (PTC), indicating a tendency

53 and catalyzing peptide bond formation at the peptidyl transferase center (PTC).

54 none molecule within its binding site in the peptidyl transferase center (PTC).

55 Thus, eIF5B interaction with the peptidyl transferase center A loop increases the accurac

56 All three are at or have been linked to the peptidyl transferase center according to the literature.

57 eptide exit tunnel at some distance from the peptidyl transferase center agrees with the proposed mod

58 contributes to the tertiary structure of the peptidyl transferase center and influences the conformat

59 he C-terminal domain of EF4 reaches into the peptidyl transferase center and interacts with the accep

60 the protein that interacts with the ribosome peptidyl transferase center and mimics the 3'-acceptor s

61 Both antibiotics bind at the peptidyl transferase center and sterically occlude the C

62 nd the subsequent expansions that shaped the peptidyl transferase center and the conserved core.

63 n of essential functional sites, such as the peptidyl transferase center and the decoding site.

64 end in tRNA interactions with the ribosomal peptidyl transferase center and the elongation factor Tu

65 ubunit along the path it follows between the peptidyl transferase center and the exit site on the dis

66 Two hydrophobic crevices, one at the peptidyl transferase center and the other at the entranc

67 a catalytic water can be coordinated in the peptidyl transferase center and, together with previous

68 d that the ribosome dynamics detected at the peptidyl transferase center are highly inhomogeneous.

69 es of tRNA substrate located in the ribosome peptidyl transferase center around the 2-fold axis, we h

70 oRNA binding causes little distortion of the peptidyl transferase center but do provide suggestive ev

71 fined to the nascent peptide residues in the peptidyl transferase center but not to the peptide segme

72 e ribosome is catalyzed in the large subunit peptidyl transferase center by release factors on recogn

73 confirm the topographical separation of the peptidyl transferase center from the E site domain.

74 bosomal subunits and the conformation of the peptidyl transferase center in the context of the intact

75 ce of these results for the structure of the peptidyl transferase center is considered.

76 ce of these results for the structure of the peptidyl transferase center is considered.

77 s to block assembly at a late stage when the peptidyl transferase center is formed, indicating a poss

78 The ribosomal peptidyl transferase center is responsible for two funda

79 gest that the positioning of Pro-tRNA in the peptidyl transferase center is the major determinant for

80 nity for CCdApPuro comparable to that of the peptidyl transferase center itself (Kd approximately 10

81 Three additional sites were at the peptidyl transferase center itself.

82 to interact with nucleotide residues in the peptidyl transferase center of domain V.

83 to pseudouridine (Psi) in a stem-loop at the peptidyl transferase center of Escherichia coli 23S rRNA

84 lmN and Cfr, both methylate A2503 within the peptidyl transferase center of prokaryotic ribosomes, yi

85 ance of pseudouridine formation (Psi) in the peptidyl transferase center of rRNA was examined by depl

86 These findings directly implicate the peptidyl transferase center of the 50S subunit in the me

87 on binding sites of the 30S subunit with the peptidyl transferase center of the 50S subunit via rRNA-

88 , inhibit protein synthesis by targeting the peptidyl transferase center of the bacterial ribosome.

89 has been implicated as a constituent of the peptidyl transferase center of the Escherichia coli 50 S

90 The action of the peptidyl transferase center of the large ribosomal unit

91 ffectively an unbranched tube connecting the peptidyl transferase center of the large subunit and the

92 lation of an adenosine nucleotide within the peptidyl transferase center of the ribosome mediated by

93 ired for introducing specific changes in the peptidyl transferase center of the ribosome that activat

94 This nucleotide is positioned within the peptidyl transferase center of the ribosome, which is a

95 of domain V, which forms a major part of the peptidyl transferase center of the ribosome.

96 catalytically productive orientation in the peptidyl transferase center of the ribosome.

97 o acids are polymerized into peptides in the peptidyl transferase center of the ribosome.

98 This modification is located in the peptidyl transferase center of the ribosome.

99 ndividual point mutations, in either the 25S peptidyl transferase center or 18S decoding site, that a

100 aphic structures of antibiotics bound to the peptidyl transferase center or the exit tunnel of archae

101 es specific nucleotides within the ribosomal peptidyl transferase center that appear to be essential

102 e essential macromolecular components of the peptidyl transferase center to 23S rRNA and ribosomal pr

103 s a hydrolytic reaction in the large subunit peptidyl transferase center to release the finished poly

104 subunit surface, connecting the tRNA in the peptidyl transferase center to the distally located nasc

105 changes occur at several nucleotides in the peptidyl transferase center upon alterations in pH, temp

106 es of 8 rRNA-modifying enzymes targeting the peptidyl transferase center were individually inactivate

107 rom the back of the 50 S particle toward the peptidyl transferase center within the 50 S subunit.

108 ming the three- dimensional structure of the peptidyl transferase center within the ribosome.

109 omain V (which is known to be a component of peptidyl transferase center) and a loop of the helix 35

110 ther universally conserved nucleotide in the peptidyl transferase center, A2451.

111 itionally, a nucleotide located close to the peptidyl transferase center, A2572, which was protected

112 the A loop and P loop, respectively, of the peptidyl transferase center, and G1735A, mapping near a

113 , including the neighborhood surrounding the peptidyl transferase center, and stable association of r

114 ons of two other nucleotide positions in the peptidyl transferase center, C2471 and U2519 (C2452 and

115 S rRNA nucleotides in the 2585 region of the peptidyl transferase center, G2583A and U2584C, were obs

116 onstruct that comprises much of the 23S rRNA peptidyl transferase center, including the central loop

117 -bound tRNAs, whose 3' termini reside in the peptidyl transferase center, label primarily nucleotides

118 in 23S rRNA, which is situated close to the peptidyl transferase center, may participate in one or m

119 Such placement, near the peptidyl transferase center, provides a rationale for th

120 discovered near the decoding center and the peptidyl transferase center, respectively.

121 mes, and their effect on conformation in the peptidyl transferase center, the GTPase-associated cente

122 erichia coli 23S rRNA, 14 are located in the peptidyl transferase center, the main antibiotic target

123 iary interactions between nucleotides in the peptidyl transferase center, the SRD, and the GTPase-ass

124 reates a free tryptophan-binding site in the peptidyl transferase center, where bound tryptophan inhi

125 as a function of the distance away from the peptidyl transferase center.

126 m to pause before allowing entrance into the peptidyl transferase center.

127 e accommodation of decoding factors into the peptidyl transferase center.

128 may generalize to other aaRS, as well as the peptidyl transferase center.

129 entified group thought to reside in the rRNA peptidyl transferase center.

130 the L27 N-terminus, which protrudes into the peptidyl transferase center.

131 to the E site but remains temporarily in the peptidyl transferase center.

132 re with another activity associated with the peptidyl transferase center.

133 n establishing the tertiary structure of the peptidyl transferase center.

134 ics on the scale of seconds at the ribosomal peptidyl transferase center.

135 is located in the immediate vicinity of the peptidyl transferase center.

136 is known to be a component of the ribosomal peptidyl transferase center.

137 oning the activated ends of tRNAs within the peptidyl transferase center.

138 ial for the successful assembly of ribosomal peptidyl transferase center.

139 via tertiary interactions to features of the peptidyl transferase center.

140 f the A-tRNA from entering the A site of the peptidyl transferase center.

141 f the acceptor end of the A-site tRNA at the peptidyl transferase center.

142 within domains IV and V, which contains the peptidyl transferase center.

143 o position helices 77 and 78 relative to the peptidyl transferase center.

144 omal tunnel to the exit port, ~100A from the peptidyl transferase center.

145 S rRNA A-loop, an essential component of the peptidyl transferase center.

146 are elongated, one residue at a time, at the peptidyl transferase center.

147 he modern ribosome this remnant includes the peptidyl transferase center.

148 esidue was separated by 14 residues from the peptidyl transferase center.

149 l RNA (rRNA) that includes hairpin 92 of the peptidyl transferase center.

150 y conserved GGQ motif packs tightly into the peptidyl transferase center.

151 ents are normally performed in the ribosomal peptidyl transferase center.

152 23S ribosomal RNA, an important part of the peptidyl transferase center.

153 somal subunit in the cleft that contains the peptidyl transferase center.

154 the RNA-mediated catalysis of the ribosomal peptidyl transferase center.

155 3S rRNA at three sites, all located near the peptidyl transferase center.

156 inhibition of TnaC-tRNA(Pro) cleavage at the peptidyl transferase center.

157 interplay between the nascent chain and the peptidyl transferase center.

158 he subunit interface, and junctions near the peptidyl transferase center.

159 f the ribosome such as the tRNA path and the peptidyl transferase center.

160 Nucleotide A2572 is in the ribosomal peptidyl transferase center.

161 binding sites of several antibiotics in the peptidyl transferase center.

162 l differences are in the conformation of the peptidyl-transferase center (PTC) and the interface betw

163 the sarcin/ricin loop (SRL) and A2531 in the peptidyl-transferase center (PTC) has adverse effects on

164 owing a disruption of the A-site side of the peptidyl-transferase center (PTC).

165 d helix 44 of 18S rRNA, domain 4 is near the peptidyl-transferase center and its helical subdomain co

166 nd peptidyl-D-aa-tRNA can trap the ribosomal peptidyl-transferase center in a conformation in which p

167 centers in the large subunit, including the peptidyl-transferase center, for unnatural polymer synth

168 tRNAs for binding in the A-site cleft in the peptidyl-transferase center, which is universally conser

169 that docks the catalytic GGQ motif into the peptidyl-transferase center.

170 s access to both the decoding center and the peptidyl-transferase center.

171 It takes place in the peptidyl transferase centre of the large (50S) ribosomal

172 nce of the appropriate A-site substrate, the peptidyl transferase centre positions the ester link of

173 wise dominantly lethal rRNA mutations in the peptidyl transferase centre that facilitate the translat

174 tituted peptidyl-tRNA substrate and that the peptidyl transferase centre undergoes a slow inactivatio

175 5 may be a constituent part of the ribosomal peptidyl transferase centre.

176 t tunnel to relay the stalling signal to the peptidyl transferase centre.

177 f RF1 that promotes its interaction with the peptidyl transferase centre.

178 nd is believed to interact directly with the peptidyl-transferase centre (PTC) of the 50S ribosomal s

179 -containing domain of RF2 interacts with the peptidyl-transferase centre (PTC).

180 itioning the conserved C-terminus within the peptidyl-transferase centre to promote recoding.

181 the protein for interaction with rRNA in the peptidyl transferase cleft of the subunit, allowing it t

182 dApPuro, a high-affinity ligand of ribosomal peptidyl transferase designed as a transition-state anal

183 minal domain with the base of the stalk, the peptidyl transferase domain, and the head of the 30 S su

184 In this method, the ribosomal peptidyl transferase forms a peptide bond between two li

185 tivate free tryptophan binding, resulting in peptidyl transferase inhibition.

186 Here, we show that the peptidyl transferase inhibitor sparsomycin triggers accu

187 Chloramphenicol, a peptidyl transferase inhibitor, affected translational f

188 ational frameshifting in the presence of the peptidyl-transferase inhibitor puromycin.

189 ledge on the fundamental mechanisms by which peptidyl transferase inhibitors modulate the catalytic a

190 y increase the susceptibility of bacteria to peptidyl transferase inhibitors.

191 lls harboring this mutation are resistant to peptidyl-transferase inhibitors (e.g., anisomycin).

192 The effects of two peptidyl-transferase inhibitors, anisomycin and sparsomy

193 The ribosomal peptidyl transferase is a biologically essential catalys

194 both aminoacyl-transfer RNA selection and in peptidyl transferase; it may also play an important role

195 toribosomes arose from two cooperating RNAs: peptidyl transferase (large subunit) and mRNA-binder (sm

196 Binding of CCdApPuro by a peptidyl transferase-like motif in the absence of protei

197 h similarity to conserved nucleotides of the peptidyl transferase loop domain of 23S rRNA and is cons

198 erferes with protein synthesis by inhibiting peptidyl transferase or the 80S ribosomal function.

199 ssential integral component of the ribosomal peptidyl transferase, oxazolidinones do not inhibit pept

200 er of macromolecular components required for peptidyl transferase, particles obtained by extraction o

201 ymes (196 nucleotides) that perform the same peptidyl transferase reaction as the ribosome: that is,

202 es are valuable for studies of the ribosomal peptidyl transferase reaction by complete kinetic isotop

203 due with the neutral pK(a) important for the peptidyl transferase reaction cannot be fully supported

204 The ribosome-catalyzed peptidyl transferase reaction displays a complex pH prof

205 tion inhibitor anisomycin, which affects the peptidyl transferase reaction in translation elongation,

206 eature will simplify characterization of the peptidyl transferase reaction mechanism.

207 or the transition state intermediate of the peptidyl transferase reaction show that this reaction pr

208 , translocation, translational accuracy, the peptidyl transferase reaction, and ribosome recycling.

209 s important mechanistic implications for the peptidyl transferase reaction.

210 active-site residues reposition to allow the peptidyl transferase reaction.

211 te acts as an "oxyanion hole" to promote the peptidyl transferase reaction.

212 h is about the same as that reported for the peptidyl transferase reaction.

213 tent with a proton shuttle mechanism for the peptidyl transferase reaction.

214 me in which ribosomal RNA is responsible for peptidyl-transferase reaction catalysis.

215 he 50S subunit of the ribosome catalyzes the peptidyl-transferase reaction of protein synthesis.

216 and specificity to G2553 of 23S rRNA and is peptidyl transferase reactive in its cross-linked state,

217 ivity of A2451 in the center of the 23S rRNA peptidyl transferase region, ascribed to a perturbed pKa

218 Haloarcula marismortui (Hma) complexed with peptidyl transferase substrate analogues that reveal an

219 Proper placement of the peptidyl transferase substrates, peptidyl-tRNA and amino

220 all peptidoglycan and are novel nonribosomal peptidyl transferases that use aminoacyl-tRNA as the ami

221 rRNA plays an important role in function of peptidyl transferase, the catalytic center of the riboso

222 The previously studied peptidyl transferase transition state analog CC-dA-phosp

223 he G-protein superfamily, catalyzes the post-peptidyl transferase translocation of deacylated tRNA an

224 -encoded peptides are in vitro inhibitors of peptidyl transferase, which is thought to be the basis f