ライフサイエンスコーパス: peptidyltransferase

1 extended FemABX family as novel nonribosomal peptidyltransferases.

2 d with sparsomycin, a known inhibitor of the peptidyltransferase A-site.

3 nd 23S rRNA, in the decoding site and in the peptidyltransferase active site of the ribosome.

4 affinities for aminoacyl-tRNA and diminished peptidyltransferase activities.

5 Peptidyltransferase activity and in vitro beta-lactamase

6 ent of anisomycin resistance and a decreased peptidyltransferase activity and increased frameshifting

7 Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the t

8 ffect ribosomal A-site associated functions, peptidyltransferase activity and subunit joining.

9 78 N-terminal residues, retained measurable peptidyltransferase activity and wild type substrate spe

10 ld interfere with PTC function by inhibiting peptidyltransferase activity and/or by restricting PTC A

11 es were shown to have essentially unimpaired peptidyltransferase activity at saturating substrate con

12 Kinetic analysis of ribosomal peptidyltransferase activity in a methanolic puromycin r

13 hat various oxazolidinones inhibit ribosomal peptidyltransferase activity in the simple reaction of 7

14 is that alterations affecting the ribosome's peptidyltransferase activity lead to changes in frameshi

15 The significant levels of peptidyltransferase activity of ribosomes with mutations

16 ucture formation that can interfere with the peptidyltransferase activity of the ribosome.

17 These changes were also found to inhibit peptidyltransferase activity, stimulating programmed -1

18 ecifically affects peptidyl-tRNA binding and peptidyltransferase activity.

19 elae, is an inhibitor of bacterial ribosomal peptidyltransferase activity.

20 We have examined the peptidyltransferase and protein synthesis activities of

21 n occur only after complex formation between peptidyltransferase and the P-site substrate.

22 though genetic data pointed to the ribosomal peptidyltransferase as the primary site of drug action,

23 In single-turnover peptidyltransferase assays, G2447A ribosomes were shown

24 In single-turnover peptidyltransferase assays, the rate of peptide bond for

25 re of an L/F-transferase and revealed that a peptidyltransferase catalyst may be constructed from app

26 osteric conformational rearrangements at the peptidyltransferase center (PTC) of the ribosome.

27 Here, two viable mutants located in the peptidyltransferase center (PTC) of yeast ribosomes were

28 ed for the peptidyl transfer activity of its peptidyltransferase center (PTC).

29 pport the hypothesis that alterations in the peptidyltransferase center affect programmed -1 ribosoma

30 n folds into a compact conformation near the peptidyltransferase center and remains folded as the seq

31 omoting Sec-tRNA(Sec) accommodation into the peptidyltransferase center and/or by stimulating the rib

32 The nascent peptide exit tunnel and peptidyltransferase center are implicated in this stalli

33 e alpha-sarcin loop has been placed near the peptidyltransferase center in Escherichia coli ribosomes

34 anges in the disposition of the AAP near the peptidyltransferase center in response to Arg.

35 ypeptide from the C terminus to the ribosome peptidyltransferase center is increased.

36 Finally, SRp38 can interact with the peptidyltransferase center of 28S rRNA, suggesting that

37 amphenicol, suggesting that Tcin targets the peptidyltransferase center of mitochondrial ribosomes.

38 0S subunit and the region below the presumed peptidyltransferase center of the 60S subunit.

39 others have shown that residue A2451 in the peptidyltransferase center of the Escherichia coli 50S r

40 n process, wherein aminoacyl-tRNA enters the peptidyltransferase center of the large ribosomal subuni

41 ow alpha-helix formation directly within the peptidyltransferase center of the ribosome interferes wi

42 rRNA, a region that constitutes part of the peptidyltransferase center of the ribosome.

43 locations of anticodon-codon interaction and peptidyltransferase center of the ribosome.

44 3, a highly conserved protein located at the peptidyltransferase center of the ribosomes, is involved

45 vestigated the effects of alterations at the peptidyltransferase center on the activity of PAP.

46 otein seems capable of contacting either the peptidyltransferase center or the decoding center, so it

47 s an essential RNA component of the ribosome peptidyltransferase center that directly interacts with

48 tide navigates along 100A of tunnel from the peptidyltransferase center to the exit port.

49 protein L27, whose N terminus may reach the peptidyltransferase center, and LepA, a protein homologo

50 approximately 38 residues from the ribosome peptidyltransferase center, and TM2-Sec61alpha photoaddu

51 large ribosomal subunit RNA belonging to the peptidyltransferase center, are encoded in all extensive

52 the elongation factor binding region and the peptidyltransferase center, facilitating coordination of

53 formation after moving 6-7 residues from the peptidyltransferase center, irrespective of loop size.

54 e structure of the active site in the E.coli peptidyltransferase center, its lack of conservation mak

55 h encodes a ribosomal protein located at the peptidyltransferase center, promote approximately three-

56 es in 25S rRNA that link the B1a bridge, the peptidyltransferase center, the GTPase-associated center

57 e to an extension domain that approaches the peptidyltransferase center.

58 r away from the protein in the A-site of the peptidyltransferase center.

59 dly when the probe was >80 residues from the peptidyltransferase center.

60 indispensable component for formation of the peptidyltransferase center.

61 es to convey pH-dependent flexibility to the peptidyltransferase center.

62 eptide (GGQ) interactions with the ribosomal peptidyltransferase center.

63 ng ribosome in the immediate vicinity of the peptidyltransferase center.

64 -tRNA binding to the P-site of the ribosomal peptidyltransferase center.

65 ides from helix 73 in domain V, close to the peptidyltransferase center.

66 ociates with the nascent chain distal to the peptidyltransferase center.

67 of the A loop must occur on docking into the peptidyltransferase center.

68 ce, in a region previously identified as the peptidyltransferase center.

69 lay pathway from the ribosomal tunnel to the peptidyltransferase centre (PTC).

70 the L/F-transferase is not a homolog of the peptidyltransferase enzymes involved in cell wall peptid

71 e bond formation, catalyzed by the ribosomal peptidyltransferase, has long been known to be sensitive

72 ing initiation, susceptibility/resistance to peptidyltransferase inhibitors, and the ability of ribos

73 bition is substrate-independent and that the peptidyltransferase itself is the oxazolidinone target.

74 ant of yeast, harboring the mak8-1 allele of peptidyltransferase-linked ribosomal protein L3 (RPL3),

75 ine to atom N6 of an adenine base within the peptidyltransferase loop of 23 S rRNA, thus conferring a

76 initiator fMet-tRNA(i)(Met) to the ribosomal peptidyltransferase P-site, which is vacant only prior t

77 and A-loops, partially overlapping with the peptidyltransferase P-site.

78 Inhibitors of the peptidyltransferase reaction (e.g. anisomycin) can trigg

79 he essential residues required to catalyze a peptidyltransferase reaction and revealed that <20% of t

80 ansferase) from Escherichia coli catalyzes a peptidyltransferase reaction that results in the N-termi

81 rapid equilibrium, ordered mechanism of the peptidyltransferase reaction, wherein binding of the A-s

82 somal frameshifting does not occur after the peptidyltransferase reaction.

83 Taken together, these results implicate the peptidyltransferase site as a regulator of both JNK/p38

84 rotein synthesis by binding to the ribosomal peptidyltransferase site.