ライフサイエンスコーパス: pericentrin

1 ent cells that was suppressed by the loss of pericentrin.

2 ction to the central region of both LIC1 and pericentrin.

3 Only the LICs coimmunoprecipitated with pericentrin.

4 ma-TuRC fractions did not contain detectable pericentrin.

5 s using antibodies to the centrosome protein pericentrin.

6 e, extracentriolar foci of gamma-tubulin and pericentrin.

7 sponding site (ALRRLLD948-L949FGD) in murine pericentrin.

8 MMP targets an integral centrosomal protein, pericentrin.

9 psies, whereas normal tissues exhibit intact pericentrin.

10 er, our results confirm that MT1-MMP cleaves pericentrin-2 in humans but not in mice and that mouse m

11 ly important, integral, centrosomal protein, pericentrin-2, was a cleavage target of MT1-MMP in human

12 and cleaved the integral centrosomal protein pericentrin-2.

13 ffect of PKCbetaII inhibition on normalizing pericentrin (a protein regulating cytokinesis), especial

14 entrally located site which colocalized with pericentrin, a component of the MTOC.

15 Pericentrin, a critical centrosome component first ident

16 rin share significant sequence homology with pericentrin, a previously identified murine centrosome c

17 Proteins that contain a Pericentrin/AKAP450 centrosomal targeting (PACT) domain

18 Immunoprecipitation of endogenous pericentrin also pulled down cytoplasmic dynein in untra

19 Here, we show that pericentrin, an integral component of the pericentriolar

20 We conclude that pericentrin anchoring of gamma tubulin complexes at cent

21 how that the centrosomal coiled-coil protein pericentrin anchors gamma TuRCs at spindle poles through

22 rosome assembly and induced modifications of pericentrin and centrin-2, two essential proteins requir

23 From these studies we conclude that pericentrin and gamma tubulin are novel dynein cargoes t

24 Centrosomes in cells with reduced levels of pericentrin and gamma tubulin have a diminished capacity

25 cent protein particles containing endogenous pericentrin and gamma tubulin move along microtubules at

26 proteins involved in microtubule nucleation, pericentrin and gamma tubulin, is inhibited in the absen

27 Pericentrin and gamma-tubulin are integral centrosome pr

28 Our results indicate that pericentrin and gamma-tubulin assemble into a unique cen

29 duces the loss of the pericentriolar markers pericentrin and gamma-tubulin from centrosomes.

30 ut not CHD4, by RNA interference dissociated pericentrin and gamma-tubulin from centrosomes.

31 ome component based its co-localization with pericentrin and gamma-tubulin within microtubule organiz

32 ity were important for proper recruitment of pericentrin and gamma-tubulin, and, ultimately, for form

33 ent of pericentriolar material, specifically pericentrin and gamma-tubulin, to the centrosome.

34 rganizing center (MTOC)-associated proteins, pericentrin and gamma-tubulin.

35 These analyses demonstrate that pericentrin and kendrin are encoded by one gene, correct

36 ion reduced the level and mislocalization of pericentrin and normalized microtubule organization in t

37 ditions associated with loss or elevation of pericentrin and propose cellular and molecular models th

38 -expressed MT1-MMP with the wild type murine pericentrin and the D948G mutant.

39 ween the mouse chromosome 10 region encoding pericentrin and the human chromosome 21 region encoding

40 letion abolishes the ability of Chk1 to bind pericentrin and to localize at centrosomes, which, in it

41 ree centrosomal antigens (hsp 73, TCP-1, and pericentrin) and the recovery of microtubule regrowth pr

42 of PCM components, including gamma-tubulin, pericentrin, and Cdk5Rap2, with centrosomes exhibiting r

43 C1 and LIC2 for the ability to interact with pericentrin, and found that only LIC1 will bind.

44 roaches led to reduced targeting of centrin, pericentrin, and ninein to the centrosome.

45 red the ability to activate MMP-2, to cleave pericentrin, and to invade the Matrigel matrix.

46 ere evaluated with respect to gamma-tubulin, pericentrin, and total tubulin polymer fractions at spec

47 Both Cdc2 and pericentrin antibodies decorate the amorphous pericentri

48 material (PCM) containing gamma-tubulin and pericentrin appear in the cell.

49 Cytoplasmic dynein, dynactin, and pericentrin are all recruited to the interphase aMTOC, a

50 organizing center proteins gamma-tubulin and pericentrin, are major sites of muscle MT nucleation, in

51 of FTIs on centrosome separation and define pericentrin as a (indirect) target of FTIs affecting cen

52 creen, we identified the centrosomal protein pericentrin as a scaffold that tethers PKC betaII to cen

53 in B1 and proteasome-mediated degradation of pericentrin as critical components in FTI-induced "donut

54 An interaction between pPKCdelta(Thr505) and pericentrin as well as gamma-tubulin was confirmed by co

55 ein, only the former of which is involved in pericentrin association with dynein.

56 function is mediated by an interaction with pericentrin at centrosomes.

57 that endogenous PKC betaII colocalizes with pericentrin at centrosomes.

58 ifically co-localizes with gamma-tubulin and pericentrin at microtubule-organizing centers (MTOCs) in

59 Studies on pericentrin at the cellular, molecular, and, more recent

60 of centriole assembly or genetic ablation of pericentrin attenuated interleukin-6, interleukin-10, an

61 Overexpression of the pericentrin-binding domain of CHD4 or another family mem

62 ganizing center containing gamma-tubulin and pericentrin, but did not regenerate centrioles.

63 ow that many NuRD components interacted with pericentrin by coimmunoprecipitation and that they local

64 Mutations in pericentrin cause the human genetic disorder Majewski/mi

65 rin (PCNT), encoding the centrosomal protein pericentrin, cause a form of osteodysplastic primordial

66 uence, and the 3' end of the published mouse pericentrin cDNA is a fragment from a different mouse ch

67 he stop codon present in the published mouse pericentrin cDNA is not found in the mouse genomic seque

68 However, comparison of the published mouse pericentrin cDNA sequence to mouse genomic DNA sequences

69 y one gene, correct the previously published pericentrin cDNA sequence, and describe the complex expr

70 proteins to MTNCs, including gamma-tubulin, pericentrin, Cep68, Cep170, and Cdk5RAP2.

71 exes with CHD3 and CHD4, but a distinct CHD3-pericentrin complex is required for centrosomal anchorin

72 Through these interactions, pericentrin contributes to a diversity of fundamental ce

73 differentiation may contribute to this, but pericentrin deficiency does not impair proximal insulin

74 Furthermore, pericentrin deficiency or mutation of a separase cleavag

75 level of PCM expansion, with a reduction in pericentrin-deficient or separase cleavage site mutant-e

76 protein remains associated with MTOCs, in a pericentrin dependent manner.

77 A and Plk1, targets them to centrosomes in a pericentrin-dependent manner, and promotes sequential ac

78 M to the nuclear DDR in which CHK1 regulates pericentrin-dependent PCM expansion to control its own a

79 ith PCNT defects and examined the effects of pericentrin depletion on insulin action using 3T3-L1 adi

80 To confirm that MT1-MMP itself cleaves pericentrin directly, rather than indirectly, we analyze

81 erexpression of the GCP2/3 binding domain of pericentrin disrupted the endogenous pericentrin-gamma T

82 ogical role for LIC, and they suggest that a pericentrin-dynein interaction in vivo contributes to th

83 ells that transiently or permanently express pericentrin exhibit severe centrosome and spindle defect

84 Pericentrin expression and localization also was determi

85 Moreover, both gamma-tubulin and pericentrin expression at MTOCs were decreased in pPKCde

86 Pericentrin expression in human tissues was profiled usi

87 Reducing pericentrin expression or ectopic expression of nonfarne

88 onstrated by continuing Ad localization with pericentrin for more than 5 h after infection, a strong

89 We conclude that pericentrin forms complexes with CHD3 and CHD4, but a di

90 or another family member (CHD3) dissociated pericentrin from centrosomes.

91 pericentrin (PCNT)--resulting in the loss of pericentrin from the centrosome, where it has key functi

92 ide a developmental basis for association of pericentrin function with interneuron defects in human s

93 To better understand pericentrin function, we overexpressed the protein in so

94 cells expressing a green fluorescent protein-pericentrin fusion protein, green fluorescent protein pa

95 The acentrosomal pole lacks pericentrin, gamma-tubulin, and centrioles, however.

96 main of pericentrin disrupted the endogenous pericentrin-gamma TuRC interaction and perturbed astral

97 The pericentrin-gamma-tubulin complex was distinct from the

98 lex is required for centrosomal anchoring of pericentrin/gamma-tubulin and for centrosome integrity.

99 ive northern blot analysis revealed that the pericentrin gene displays a complex expression pattern i

100 Although pericentrin has been known to be up-regulated in epithel

101 ed in fission yeast by overexpression of the pericentrin homolog Pcp1p.

102 d that PKA catalytic activity is enriched in pericentrin immunoprecipitates.

103 Knockdown of pericentrin in adipocytes had no effect on proximal insu

104 o-localizes with gamma-tubulin, centrin, and pericentrin in centrosomes.

105 Depletion of pericentrin in neural progenitors phenocopies effects of

106 y elevating levels of the centrosome protein pericentrin in prostate epithelial cell lines reproduces

107 MT1-MMP was colocalized with pericentrin in the centrosomal compartment and especiall

108 of MT1-MMP activity correlates with degraded pericentrin in tumor biopsies, whereas normal tissues ex

109 teins, pericentriolar material 1 (PCM-1) and pericentrin, in primary human fibroblasts.

110 centrosomal recruitment of gamma-tubulin and pericentrin, interferes with microtubule organization, d

111 Pericentrin is a conserved protein of the centrosome inv

112 Pericentrin is a critical centrosomal protein required f

113 , these results provide strong evidence that pericentrin is an AKAP in vivo.

114 Pericentrin is an integral centrosomal component that an

115 Pericentrin is an integral component of the centrosome t

116 Pericentrin is known to be essential to the normal funct

117 Whereas the localization of pericentrin is not affected, alpha- and gamma-tubulin lo

118 These findings indicate that pericentrin is required for proper migration of olfactor

119 Additionally, overexpressed pericentrin is seen to interact with endogenous LIC1 exc

120 We found that pericentrin is up-regulated in human tumor endothelium c

121 ytic subunit of PKA coimmunoprecipitate with pericentrin isolated from HEK-293 cell extracts and that

122 evels of Pcp1, the fission yeast ortholog of pericentrin/kentrin, and reducing pcp1(+) expression sig

123 The effect of pericentrin knockdown on insulin action and adipogenesis

124 Cdc2 and to the centrosome-specific protein, pericentrin, label the centrosome in an apparently cell

125 Here we show that fission-yeast pericentrin-like Pcp1 regulates multiple functions of th

126 We identify pericentrin-like protein (PLP) as a negative regulator o

127 We identify an unprecedented role for Pericentrin-like protein (PLP), which localizes to the t

128 domain protein in Drosophila (the Drosophila pericentrin-like protein [D-PLP]) is associated with bot

129 Here we use Drosophila pericentrin-like-protein (PLP) to understand how the PAC

130 ed Gle1 levels are correlated with decreased pericentrin localization at the centrosome and microtubu

131 and dynactin, are lost from centrosomes, but pericentrin localization persists.

132 ction to the pericentriolar material protein pericentrin, loss of function of which is associated wit

133 the complexity of phenotypes associated with pericentrin-mediated disorders is somewhat daunting, ins

134 l phenotypes were significantly reduced in a pericentrin mutant with diminished GCP2/3 binding and we

135 entrioles but cannot assemble gamma-tubulin, pericentrin, or other pericentriolar proteins into an or

136 includes the proteins Spc42, Spc110 (kendrin/pericentrin ortholog), calmodulin (Cmd1), Spc29, and Cnm

137 Spindles in pericentrin-overexpressing cells were disorganized and m

138 Like pericentrin, p-MARCKS staining at the MI spindle poles w

139 The large coiled-coil protein, pericentrin, participates in PCM assembly and has been i

140 alization of centrosomal proteins, including Pericentrin, Pcm1, and gamma-tubulin, depends on Nesprin

141 Here, we show that the centrosome protein pericentrin (Pcnt) colocalizes with IFT proteins to the

142 t and premature termination mutations in the pericentrin (PCNT) gene were identified in individuals w

143 we identified a frame shift mutation in the pericentrin (Pcnt) gene.

144 As in MOPDII patients, disruption of pericentrin (Pcnt) in mice caused a number of abnormalit

145 s caused by mutations in the centrosome gene pericentrin (PCNT) that lead to severe pre- and postnata

146 Genetic defects in human pericentrin (PCNT), encoding the centrosomal protein per

147 w report that mutations in the gene encoding pericentrin (PCNT)--resulting in the loss of pericentrin

148 ), myosin IIB (MYOIIB), aPKCzeta, LGL, PAR3, pericentrin, PROM1] is lost.

149 The centrosomal pericentrin-related proteins play pivotal roles in vario

150 n by expression of the interacting region of pericentrin results in release of PKC from the centrosom

151 corresponds to the 3' end for a 7.1-kb mouse pericentrin RNA encoded on chromosome 10.

152 Germaine to pericentrin's function in organizing PCM is its ability

153 We found that cells depleted of PCM-1 or pericentrin show lower levels of markers for S phase and

154 Pericentrin silencing by small interfering RNAs in somat

155 Additionally, pericentrin silencing or overexpression induced G2/antep

156 as confirmed by (i) the assembly of multiple pericentrin-stained centrioles at the apical surface, (i

157 In addition, virtually all pericentrin-staining structures in tumor cells nucleated

158 nein coimmunoprecipitated with overexpressed pericentrin, suggesting that the motor was sequestered i

159 Recent studies link pericentrin to a growing list of human disorders.

160 nt with these observations is the ability of pericentrin to cosediment with taxol-stabilized microtub

161 n that recruits Spc110, a protein related to pericentrin, to the SPB.

162 To define the basis for this interaction, pericentrin was coexpressed with cytoplasmic dynein heav

163 Pericentrin was highly expressed in human skeletal muscl

164 recently identified, but its relationship to pericentrin was not clear.

165 sitive to MT1-MMP, whereas unmodified murine pericentrin was resistant to proteolysis.

166 hat exhibited the cleavage sequence of human pericentrin was sensitive to MT1-MMP, whereas unmodified

167 In a yeast two-hybrid screen with pericentrin we identified chromodomain helicase DNA-bind

168 along with the centrosome-specific protein, pericentrin, were also present within an enriched prepar

169 he LICs: binding to the centrosomal protein, pericentrin, which represents a novel, non-dynactin-base

170 somal proteins, including Akap450, Pcm1, and Pericentrin, whose association with Nesprin-1alpha is in

171 The interaction of pericentrin with RII is mediated through a binding domai

172 ed by the C1A domain of PKC and a segment of pericentrin within residues 494-593.