ライフサイエンスコーパス: perilipin

1 calizes with the known lipid droplet protein perilipin.

2 PGC-1alpha and UCP-2, and down-regulation of perilipin.

3 ogue of the vertebrate lipid-storage protein perilipin.

4 phosphorylating hormone-sensitive lipase and perilipin.

5 e major lipid storage site and is defined by perilipin.

6 cilitate the PKA-mediated phosphorylation of perilipin.

7 requires the phosphorylation of both HSL and perilipin.

8 se of the loss of the protective function of perilipin.

9 ession levels of hormone-sensitive lipase or perilipin.

10 ts major lipid droplet-associated substrate, perilipin.

11 xpressed in 3T3-L1 preadipocytes, which lack perilipins.

12 -length perilipin A or control cells lacking perilipins.

13 protein, ADRP, and more distantly related to perilipins.

14 3T3-L1 pre-adipocytes that normally lack the perilipins.

15 ctions of ATGL and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differen

16 cytosolic hormone-sensitive lipase (HSL) and perilipin 1 (Plin1) in the lipid droplet by protein kina

17 se (FAS), hormone sensitive lipase (HSL) and perilipin 1 (PLIN1), was examined in the present study.

18 Phosphorylation of perilipin 1 disrupts these interactions and mobilizes CG

19 Furthermore, knockdown of perilipin 1 in adipocytes leads to replacement of perili

20 on affecting the carboxy-terminus (439fs) of perilipin 1 in two unrelated families.

21 Perilipin 1 is a lipid droplet coat protein predominantl

22 In contrast, neither perilipin 1 nor 2 interacted directly with ATGL.

23 Collectively these data suggest that whereas perilipin 1 potently suppresses basal lipolysis in adipo

24 mplementation studies suggested that whereas perilipin 1 prevents the activation of adipose tissue tr

25 ns between CGI-58 and the LD coating protein perilipin 1 restrain the ability of CGI-58 to activate A

26 served region within the carboxy terminus of perilipin 1 that binds and stabilizes ABHD5 by retarding

27 generated by fusing the carboxy terminus of perilipin 1 to the amino terminus of perilipins 2 or 3 s

28 o ubiquitinated proteins, possibly including perilipin 1, on LDs.

29 The discovery by Dr. Constantine Londos of perilipin 1, the major scaffold protein at the surface o

30 Perilipin 1, the most abundant lipid-coat protein in adi

31 basal lipolysis as effectively as wild-type perilipin 1.

32 ssues express perilipins 2 or 3, rather than perilipin 1.

33 We designate PPE15 as mycobacterial perilipin-1 (MPER1).

34 ) interacts with perilipin-5 (Plin5) but not perilipin-1 (Plin1).

35 mino acid sequence identities with mammalian perilipin-1 and was upregulated in Mtb dormancy.

36 ipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-related protein), a

37 (adenovirus [Ad]-PLIN5) and those expressing perilipin 2 (PLIN2) (Ad-PLIN2) had higher [(3)H]FA incor

38 ivity are related to increased expression of perilipin 2 (PLIN2) and perilipin 5 (PLIN5).

39 Perilipin 2 (PLIN2) is a lipid-droplet protein that is u

40 usly reported that the lipid droplet protein perilipin 2 (PLIN2) modulates lipid homeostasis in the l

41 ipose differentiation-related protein (Adrp)/perilipin 2 (Plin2), and investigated its effects on ath

42 Perilipin 2 (PLIN2/adipose differentiation-related prote

43 ipin 1 in adipocytes leads to replacement of perilipin 2 on LDs.

44 In contrast, perilipin 2, a lipid droplet-stabilizing protein, is pro

45 Perilipin 2, which coats lipid droplets, is markedly ind

46 that the sequestration of hepatic lipids in perilipin 2-coated droplets ameliorates insulin resistan

47 r, AB-hydrolase domain containing-5 (ABHD5), perilipins 2 and 3 do so less effectively.

48 ly suppresses basal lipolysis in adipocytes, perilipins 2 and 3 facilitate higher rates of basal lipo

49 We sought to understand the role of perilipins 2 and 3 in regulating basal lipolysis.

50 inus of perilipin 1 to the amino terminus of perilipins 2 or 3 stabilize ABHD5 and suppress basal lip

51 These tissues express perilipins 2 or 3, rather than perilipin 1.

52 ess basal lipolysis more effectively than WT perilipins 2 or 3.

53 specificity of urine aquaporin-1 (AQP1) and perilipin-2 (PLIN2) concentrations as unique, noninvasiv

54 Perilipin-2 (PLIN2) is a constitutively associated cytop

55 Mice with or without whole-body deletion of perilipin-2 (Plin2-null) were fed either Western or cont

56 ther verified by the increased expression of perilipin-2 and decreased activity of hormone-sensitive

57 Perilipin-2 deletion prevents obesity and insulin resist

58 reased lipid droplet accumulation, increased perilipin-2 expression, and decreased HSL activity.

59 converting it to triacylglycerol, attenuated perilipin 3 DG-induced ER recruitment.

60 ing trafficking with AlF(4)(-) redistributed perilipin 3 differently under conditions of triacylglyce

61 he DG lipase inhibitor RHC80267 enhanced the perilipin 3 recruited to lipid droplets and raised DG le

62 ion with triacsin C, enhanced recruitment of perilipin 3 to the ER.

63 cells with a membrane-permeable DG recruited perilipin 3 to the ER.

64 acid-induced triacylglycerol synthesis drove perilipin 3 to the tubular ER.

65 in 3, but when added together with AlF(4)(-) perilipin 3 was recruited to lipid droplets rather than

66 ates adenylate cyclase, did not redistribute perilipin 3, but when added together with AlF(4)(-) peri

67 We show that perilipin 3- and perilipin 4-coated lipid droplets emerg

68 rolytic product, affects the distribution of perilipin 3.

69 l droplets with a protein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12.

70 ting protein of 47 kDa (TIP47; also known as perilipin-3 and mannose-6-phosphate receptor-binding pro

71 Membrane-permeable DG also drove perilipin 4 and 5 onto the ER.

72 We show that perilipin 3- and perilipin 4-coated lipid droplets emerge along the endop

73 ein coat that includes perilipin 3/TIP47 and perilipin 4/S3-12.

74 dipose differentiation-related protein), and perilipin 5 (LSDP5) using multiple techniques as follows

75 r hypothesis that lipid droplet (LD) protein perilipin 5 (PLIN5) in beta-cells aids PP insulin secret

76 physiological studies related to the role of perilipin 5 (Plin5) in regulating lipid droplet accumula

77 Perilipin 5 (PLIN5) is a lipid droplet protein and is hi

78 M, trained athletes possess higher levels of perilipin 5 (PLIN5), a lipid droplet (LD) coating protei

79 tients, Trained subjects have high levels of perilipin 5 (PLIN5).

80 reased expression of perilipin 2 (PLIN2) and perilipin 5 (PLIN5).

81 Cells expressing both ectopic perilipin 5 and ATGL showed a 3-fold increase in lipolys

82 Here we identify the lipid droplet protein Perilipin 5 as a catecholamine-triggered interaction par

83 Our studies establish perilipin 5 as a novel ATGL partner and provide evidence

84 sed [(32)P]orthophosphate incorporation into perilipin 5 by 2-fold, whereas neither ATGL nor CGI-58 w

85 lipolysis, whereas interaction of ATGL with perilipin 5 decreased lipolysis.

86 improper delivery, potentially via decreased perilipin 5 expression and mitochondrial-LD tethering, a

87 We propose that Perilipin 5 is an important molecular link that couples

88 t during catecholamine-stimulated lipolysis, Perilipin 5 is phosphorylated by protein kinase A and fo

89 Perilipin 5 promotes PGC-1alpha co-activator function by

90 ss of function studies in cells that nuclear Perilipin 5 promotes transcription of genes that mediate

91 ript levels for the mitochondrial-LD tether, perilipin 5, in the failing myocardium (P = 0.003).

92 lts show that ATGL interacts with CGI-58 and perilipin 5; the latter is selectively expressed in oxid

93 se triglyceride lipase (Atgl) interacts with perilipin-5 (Plin5) but not perilipin-1 (Plin1).

94 The expression of OXPAT/perilipin-5, adipose triglyceride lipase, and stearoyl-C

95 tion of the lipid droplet-associated protein perilipin A (Peri A) mediates the actions of cyclic AMP-

96 kinase A (PKA)-dependent phosphorylation of perilipin A (Peri A), an essential lipid droplet (LD)-as

97 location of hormonesensitive lipase (HSL) to perilipin A (Plin)-containing droplets and increases the

98 d lipase, we conclude that the expression of perilipin A alone is sufficient to confer PKA-mediated l

99 h a decrease in total cellular expression of perilipin A and B, consistent with the hypothesis that a

100 ile much larger and stable increases in both perilipin A and C proteins were observed.

101 Full-length perilipin A associates with lipid droplets via hydrophob

102 hydrolysis in adipocytes; phosphorylation of perilipin A by protein kinase A facilitates maximal lipo

103 Perilipin A coats the lipid storage droplets in adipocyt

104 A peptide composed of the central domain of perilipin A directed a fused green fluorescent protein t

105 lipid droplet and cytoplasmic pools, whereas perilipin A does not.

106 A-mediated phosphorylation of serine 492 of perilipin A drives the fragmentation and dispersion of l

107 but activation of PKA and phosphorylation of perilipin A engenders a 7-fold lipolytic activation.

108 FP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated l

109 d droplets, we stably expressed fragments of perilipin A in 3T3-L1 fibroblasts.

110 The precocious expression of full-length perilipin A in 3T3-L1 preadipocytes aided more rapid sto

111 oxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage.

112 e roles of the amino and carboxyl termini of perilipin A in facilitating triacylglycerol storage.

113 We conclude that perilipin A increases the triacylglycerol content of cel

114 Perilipin A inhibits triacylglycerol hydrolysis by 87% w

115 Perilipin A is a key regulator of triacylglycerol storag

116 inal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipo

117 dicate that the unique C-terminal portion of perilipin A is responsible for its protection against li

118 Perilipin A is the most abundant lipid droplet-associate

119 droplets coated with perilipin B or mutated perilipin A lacking this sequence.

120 s using sucrose gradients confirmed that the perilipin A localized exclusively to lipid droplets.

121 escence microscopy revealed that the ectopic perilipin A localized to the surfaces of the tiny cluste

122 Epitope-tagged perilipin A mRNAs were efficiently loaded with polyribos

123 Adenovirus-mediated overexpression of perilipin A or B maintains perilipin protein levels on t

124 ed with that of cells expressing full-length perilipin A or control cells lacking perilipins.

125 CGI-58 binds to lipid droplets coated with perilipin A or mutated forms of perilipin with an intact

126 In contrast, overexpression of perilipin A or perilipin B does not inhibit isoprotereno

127 These studies suggest that perilipin A plays a major role in the regulation of tria

128 hus, we conclude that the central 25% of the perilipin A sequence contains all of the amino acids nec

129 Cells expressing perilipin A stored 6-30-fold more triacylglycerol than c

130 previously shown that the central region of perilipin A targets and anchors it to lipid droplets, at

131 imes slower in lipid-loaded cells expressing perilipin A than in lipid-loaded control cells, when tri

132 The mutation of serine 492 of perilipin A to alanine prevented the dispersion of clust

133 that the sequences responsible for anchoring perilipin A to lipid droplets are most likely domains of

134 To map the domains that target and anchor perilipin A to lipid droplets, we stably expressed fragm

135 the carboxyl terminus is required to target perilipin A to lipid droplets; however, there are multip

136 protein kinase A-mediated phosphorylation of perilipin A triggers the remodeling of lipid droplets.

137 erminus of 112 amino acids that is unique to perilipin A were critical to facilitate triacylglycerol

138 boxyl-terminal truncation mutations of mouse perilipin A were stably expressed in 3T3-L1 preadipocyte

139 substitution of serines 433, 492, and 517 of perilipin A with glutamic acid residues blocked the disp

140 L and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-relat

141 oplet function was differentially increased (perilipin A), reduced severalfold (adipose differentiati

142 the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (AD

143 ipid accumulation (hormone-sensitive lipase, perilipin A, and LDs).

144 ced a transient 6-fold increase in levels of perilipin A, but not C, mRNA, while much larger and stab

145 ltured fibroblasts stably expressing ectopic perilipin A, clustered lipid droplets disperse throughou

146 in cells that express fully phosphorylatable perilipin A, confirming that perilipin is required to el

147 d droplets, while in cells stably expressing perilipin A, the lipid droplets were more numerous and t

148 To investigate perilipin function, perilipin A, the predominant isoform, was ectopically ex

149 amster ovary cells that express both HSL and perilipin A, these two proteins cooperate to produce a m

150 otein levels of hormone-sensitive lipase and perilipin A, two proteins involved in adipocyte lipolysi

151 Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is de

152 d droplets to fragment into myriad dispersed perilipin A-covered microlipid droplets.

153 roduction of GFP-tagged HSL with and without perilipin A.

154 both protein kinase A-phosphorylated HSL and perilipin A.

155 lycerol lipase activity by the expression of perilipin A.

156 creased expression of cytoplasmic lipids and perilipin A.

157 major splice variants of the perilipin gene, perilipins A and B, in Chinese hamster ovary fibroblasts

158 investigated the intracellular targeting of perilipin, a major lipid coat protein, and hormone-sensi

159 tein, colocalizes around lipid droplets with perilipin, a regulator of lipolysis.

160 Perilipins, a family of phosphoproteins, are specificall

161 of PKA sites within the N-terminal region of perilipin abrogates the PKA-mediated lipolytic response.

162 mbryonic fibroblasts stably transfected with perilipin accumulated approximately 4.5-fold less lipid

163 lipid droplet-associated PAT protein family (perilipin, ADFP, and Tip47).

164 Lipid droplet proteins of the PAT (perilipin, adipophilin, and TIP47) family regulate cellu

165 receptor-binding protein 1), a member of the perilipin, adipophilin, TIP47 (PAT) family of proteins i

166 olutionarily related family of PAT proteins (Perilipin, Adipophilin, TIP47), which is defined by sequ

167 lly contains at least one member of the PAT (perilipin, adipose differentiation-related protein, and

168 LDs are coated with perilipin, adipose differentiation-related protein, tail

169 The mammalian proteins Perilipin, ADRP, and TIP47 share extensive amino acid se

170 However, while Perilipin and ADRP localize exclusively to neutral lipid

171 Further, leptin decreased perilipin and fatty acid synthase expression, which play

172 ipid droplet fission, and phosphorylation of perilipin and hormone sensitive lipase - all hallmarks o

173 f attack by hormone-sensitive lipase, and 3) perilipin and hormone-sensitive lipase are continuously

174 Compared to controls, perilipin and leptin mRNA concentrations were lower (P <

175 c agonists triggers rapid phosphorylation of perilipin and translocation of hormone-sensitive lipase

176 into NIH 3T3 fibroblasts lacking endogenous perilipins and HSL but overexpressing acyl-CoA synthetas

177 ession of adipogenic markers (aP2, CD36, and perilipin) and low fatty-acid synthase enzymatic activit

178 CAAT/enhancer-binding protein (C/EBP) alpha, perilipin, and adipocyte-specific fatty acid-binding pro

179 FoxC2 inhibits the expression of C/EBPalpha, perilipin, and adiponectin even in the presence of poten

180 cell, most notably C/EBPalpha, adiponectin, perilipin, and the adipose-specific fatty acid-binding p

181 the adipocyte-specific lipid droplet protein perilipin, and the dead/dying adipocytes are surrounded

182 with mRNA levels of lipid droplet proteins, perilipin, and TIP47 in human subcutaneous adipose tissu

183 tissue and testes, tissues that also express perilipins, and at lower levels in liver, skin, kidney,

184 Both HSL and perilipin are substrates for polyphosphorylation by prot

185 The perilipins are the most abundant proteins at the surface

186 The perilipins are the most abundant proteins coating the su

187 ipin in adipose lipid metabolism and suggest perilipin as a potential target for attacking problems a

188 ide evidence that the protein composition of perilipins at the LD surface regulates lipolytic activit

189 indings, but also showed co-precipitation of perilipin B and CGI-58.

190 n contrast, overexpression of perilipin A or perilipin B does not inhibit isoproterenol-stimulated li

191 In contrast, perilipin B exerts only minimal protection against lipol

192 o 429, but not to lipid droplets coated with perilipin B or mutated perilipin A lacking this sequence

193 e distinct from those droplets surrounded by perilipin; but by 240 min, most perilipin-coated droplet

194 in ligand-induced complex formation between perilipin, caveolin-1, and the catalytic subunit of PKA

195 Like perilipin, Cidea and the related lipid droplet protein C

196 urrounded by perilipin; but by 240 min, most perilipin-coated droplets had some S3-12 on the surface

197 synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood.

198 st of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes.

199 Perilipin coats the lipid droplets of adipocytes and is

200 umans, expression of Cidea, Cidec/FSP27, and perilipin correlates positively with insulin sensitivity

201 ases to substrate accessibility, mediated by perilipin-dependent protein sequestration and recruitmen

202 Perilipins drive triacylglycerol storage in adipocytes b

203 Perilipin (encoded by the gene Plin), an adipocyte prote

204 Thus, perilipin expression and phosphorylation state are criti

205 that the core functions of Pet10p and other perilipins extend beyond protection from lipases and inc

206 ted amino- or carboxyl-terminal mutations of perilipin failed to serve a dominant negative function i

207 The perilipin family is of ancient origin and has expanded t

208 Members of the perilipin family of lipid droplet scaffold proteins are

209 rator-activated receptor gamma, adiponectin, perilipin, fatty acid synthase, and lipoprotein lipase.

210 These ML adipocytes expressed adiponectin, perilipin, fatty acid-binding protein (FABP), leptin, C/

211 treatment caused an electrophoretic shift of perilipin from 65 to 67 kDa, consistent with perilipin h

212 alter the isoproterenol-induced migration of perilipins from the lipid droplet.

213 of the cells was quantified as a measure of perilipin function and was compared with that of cells e

214 To investigate perilipin function, perilipin A, the predominant isoform

215 se results provide the first evidence of how perilipin functions and suggest that TNF-alpha regulates

216 orms of the two major splice variants of the perilipin gene, perilipins A and B, in Chinese hamster o

217 The Perilipin homologue LSD2 has been identified as a regula

218 perilipin from 65 to 67 kDa, consistent with perilipin hyperphosphorylation by activated cAMP-depende

219 t with the hypothesis that TNF-alpha induces perilipin hyperphosphorylation by activating PKA, TNF-al

220 The data reveal a major role for perilipin in adipose lipid metabolism and suggest perili

221 Our results demonstrate a role for perilipin in reining in basal HSL activity and regulatin

222 To study the role of perilipin in vivo, we have created a perilipin knockout

223 lipolysis in adipocytes suggests a role for perilipins in this process.

224 ted disruption of the lipid droplet protein, perilipin, in mice leads to constitutional lipolysis ass

225 tion facilitates (either direct or indirect) perilipin interaction with LD-associated HSL.

226 Perilipin is a member of the evolutionarily related fami

227 imulated lipolytic activity, indicating that perilipin is required for maximal lipolytic activity.

228 hosphorylatable perilipin A, confirming that perilipin is required to elicit the HSL translocation re

229 otein kinase A (PKA), and phosphorylation of perilipin is required to induce HSL to translocate from

230 jor control over HSL-mediated lipolysis when perilipin is the main lipid droplet protein.

231 nerated from murine embryonic fibroblasts of perilipin knock-out mice.

232 role of perilipin in vivo, we have created a perilipin knockout mouse.

233 pported by adenoviral expression of a mutant perilipin lacking all six PKA sites (Peri Adelta1-6).

234 e culture medium; therefore, the increase in perilipin levels in the presence of fatty acids is likel

235 This study describes the dependence of perilipin levels on neutral lipid storage in cultured Y-

236 ipid droplet (LD) numbers and alterations in perilipin levels, supporting a role for spartin in LD ma

237 termined for hormone sensitive lipase (HSL), perilipin (lipid droplet-associated protein), and two ad

238 Perilipins localize to lipid droplet surfaces and displa

239 Similar to its mammalian counterpart Perilipin, LSD2 is responsible for regulating lipid home

240 Recent reports reveal a function for perilipins, major lipid droplet proteins in adipocytes,

241 energy balance; thus, agents that inactivate perilipin may prove useful as anti-obesity medications.

242 Universally, the perilipins modulate cellular lipid storage.

243 Perilipin null (peri(-/-)) and wild-type (peri(+/+)) mic

244 When fed a high-fat diet, the perilipin null animals are resistant to diet-induced obe

245 ineered from murine embryonic fibroblasts of perilipin null mice (Peri-/- MEF), we demonstrate by cel

246 Isolated adipocytes of perilipin null mice exhibit elevated basal lipolysis bec

247 rmed in differentiated brown adipocytes from perilipin null mice expressing an adipose-specific Peri

248 se to the constitutive lipolysis, we studied perilipin-null (plin(-/-)) mice in terms of their fatty

249 Adipocytes from perilipin-null animals have an elevated basal rate of li

250 ocytes derived from embryonic fibroblasts of perilipin-null mice.

251 ith lipid droplet surfaces at a low level in perilipin nulls, but that stimulated translocation from

252 interactions, as shown by the persistence of perilipins on lipid droplets after centrifugation throug

253 tances, a set of structural proteins such as perilipin or adipose differentiation-related protein, en

254 To assess whether perilipins participate directly in PKA-mediated lipolysi

255 Adipophilin (ADPH/Plin2), a member of the perilipin/PAT family of lipid droplet-associated protein

256 Perilipin (Peri) A is a lipid droplet-associated phospho

257 Perilipin (Peri) A is a phosphoprotein located at the su

258 This suggests that PKA-dependent perilipin phosphorylation facilitates (either direct or

259 It is believed that perilipin phosphorylation is essential for the transloca

260 defects in white fat were caused by impaired perilipin phosphorylation, and the reduced triglyceride

261 HSL at the LD surface requires PKA-dependent perilipin phosphorylation.

262 induced HSL translocation was independent of perilipin phosphorylation.

263 nd of the hormone-sensitive lipase (HSL) and perilipin phosphorylation.

264 whether the presence of polymorphisms at the perilipin (PLIN) locus (PLIN1, 6209T-->C; PLIN4, 11482G-

265 Several perilipin (PLIN) polymorphic sites have been studied for

266 Perilipin (PLIN) proteins constitute an ancient family i

267 T: Because the lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyce

268 EY POINTS: The lipid droplet (LD)-associated perilipin (PLIN) proteins promote intramuscular triglyce

269 fat cells, and there is strong evidence that perilipin (Plin), a lipid droplet scaffold, and adipose

270 hagosome or autophagolysosome formation, and perilipin (PLIN), a lipid droplet-associated protein, su

271 ported that the inactivation of the gene for perilipin (plin), an adipocyte lipid droplet surface pro

272 Perilipins (PLINs) play a key role in energy storage by

273 orescently tagged proteins indicate that: 1) perilipin preferentially targets a special class of peri

274 ter assay and induced lipid accumulation and perilipin protein expression in BMS2 cells.

275 stent with the hypothesis that a decrease in perilipin protein expression is required for TNF-alpha-i

276 s are coated with one or more members of the perilipin protein family, which serve important function

277 regulates lipolysis, in part, by decreasing perilipin protein levels at the lipid droplet surface.

278 overexpression of perilipin A or B maintains perilipin protein levels on the lipid droplet and blocks

279 ocytes of OVX-E2 mice contained >3-fold more perilipin protein than adipocytes of pairfed control (OV

280 let translocation or ABHD5 interactions with perilipin proteins and ABHD5 ligands, demonstrating that

281 We investigated mechanisms by which three perilipin proteins control lipolysis by adipocyte trigly

282 Analysis of chimeric and mutant perilipin proteins demonstrated that amino acids 200-463

283 redefine and expand our understanding of how perilipin regulates HSL-mediated lipolysis in adipocytes

284 In mammals, perilipin regulates lipid droplet homeostasis but no suc

285 eins identified in both preparations include perilipin, S3-12, vimentin, and TIP47; in contrast, adip

286 d lipolysis and the electrophoretic shift of perilipin, suggesting a role for PKA in TNF-alpha-induce

287 raction between hormone-sensitive lipase and perilipin, the protein that coats the adipocyte lipid dr

288 Perilipins, the major structural proteins coating the su

289 Perilipins, the most abundant proteins on these lipid dr

290 ion with TNF-alpha and MEK inhibitors caused perilipin to migrate as a single 65-kDa band.

291 review provides a summary that connects the perilipins to both cellular and whole-body homeostasis.

292 lated approximately 4.5-fold less lipid than perilipin-transfected wild-type cells.

293 in fibroblasts stably expressing the mutated perilipin upon incubation with forskolin and IBMX.

294 -L1 cells stably expressing mutated forms of perilipin using microscopy.

295 in-1 null adipocytes, the phosphorylation of perilipin was dramatically reduced, indicating that cave

296 tion of lipolysis associated with absence of perilipin, WAT activated pathways to rid itself of the p

297 kinase 1 (ERK1) and ERK2 and to downregulate perilipin (which has been implicated in the lipolytic ef

298 sitive lipase (HSL), a cytosolic enzyme, and perilipin, which coats the lipid droplet surface in adip

299 found in a wide array of cell types, and the perilipins, which are restricted to adipocytes and stero

300 ontains a PAT domain, a defining property of perilipins, which was not previously known to exist in y

301 coated with perilipin A or mutated forms of perilipin with an intact C-terminal sequence from amino