ライフサイエンスコーパス: perilipin A

1 creased expression of cytoplasmic lipids and perilipin A.

2 roduction of GFP-tagged HSL with and without perilipin A.

3 both protein kinase A-phosphorylated HSL and perilipin A.

4 lycerol lipase activity by the expression of perilipin A.

5 the mechanisms by which PAT family members, perilipin A, adipose differentiation-related protein (AD

6 d lipase, we conclude that the expression of perilipin A alone is sufficient to confer PKA-mediated l

7 h a decrease in total cellular expression of perilipin A and B, consistent with the hypothesis that a

8 ile much larger and stable increases in both perilipin A and C proteins were observed.

9 major splice variants of the perilipin gene, perilipins A and B, in Chinese hamster ovary fibroblasts

10 ich lipid storage droplets are encoated with perilipins A and C.

11 ipid accumulation (hormone-sensitive lipase, perilipin A, and LDs).

12 Full-length perilipin A associates with lipid droplets via hydrophob

13 ced a transient 6-fold increase in levels of perilipin A, but not C, mRNA, while much larger and stab

14 hydrolysis in adipocytes; phosphorylation of perilipin A by protein kinase A facilitates maximal lipo

15 ltured fibroblasts stably expressing ectopic perilipin A, clustered lipid droplets disperse throughou

16 Thus, CGI-58 binds to perilipin A-coated lipid droplets in a manner that is de

17 Perilipin A coats the lipid storage droplets in adipocyt

18 in cells that express fully phosphorylatable perilipin A, confirming that perilipin is required to el

19 d droplets to fragment into myriad dispersed perilipin A-covered microlipid droplets.

20 A peptide composed of the central domain of perilipin A directed a fused green fluorescent protein t

21 lipid droplet and cytoplasmic pools, whereas perilipin A does not.

22 A-mediated phosphorylation of serine 492 of perilipin A drives the fragmentation and dispersion of l

23 but activation of PKA and phosphorylation of perilipin A engenders a 7-fold lipolytic activation.

24 FP or LSDP5; in contrast, phosphorylation of perilipin A exerts the major control over HSL-mediated l

25 Perilipins, a family of phosphoproteins, are specificall

26 d droplets, we stably expressed fragments of perilipin A in 3T3-L1 fibroblasts.

27 The precocious expression of full-length perilipin A in 3T3-L1 preadipocytes aided more rapid sto

28 oxyl termini are critical to the function of perilipin A in facilitating triacylglycerol storage.

29 e roles of the amino and carboxyl termini of perilipin A in facilitating triacylglycerol storage.

30 We conclude that perilipin A increases the triacylglycerol content of cel

31 Perilipin A inhibits triacylglycerol hydrolysis by 87% w

32 Perilipin A is a key regulator of triacylglycerol storag

33 inal protein kinase A consensus sequences of perilipin A is required for HSL binding and maximal lipo

34 dicate that the unique C-terminal portion of perilipin A is responsible for its protection against li

35 Perilipin A is the most abundant lipid droplet-associate

36 droplets coated with perilipin B or mutated perilipin A lacking this sequence.

37 s using sucrose gradients confirmed that the perilipin A localized exclusively to lipid droplets.

38 escence microscopy revealed that the ectopic perilipin A localized to the surfaces of the tiny cluste

39 investigated the intracellular targeting of perilipin, a major lipid coat protein, and hormone-sensi

40 Epitope-tagged perilipin A mRNAs were efficiently loaded with polyribos

41 Adenovirus-mediated overexpression of perilipin A or B maintains perilipin protein levels on t

42 ed with that of cells expressing full-length perilipin A or control cells lacking perilipins.

43 CGI-58 binds to lipid droplets coated with perilipin A or mutated forms of perilipin with an intact

44 In contrast, overexpression of perilipin A or perilipin B does not inhibit isoprotereno

45 tion of the lipid droplet-associated protein perilipin A (Peri A) mediates the actions of cyclic AMP-

46 kinase A (PKA)-dependent phosphorylation of perilipin A (Peri A), an essential lipid droplet (LD)-as

47 L and its co-lipase CGI-58 with perilipin 1 (perilipin A), perilipin 2 (adipose differentiation-relat

48 These studies suggest that perilipin A plays a major role in the regulation of tria

49 location of hormonesensitive lipase (HSL) to perilipin A (Plin)-containing droplets and increases the

50 oplet function was differentially increased (perilipin A), reduced severalfold (adipose differentiati

51 tein, colocalizes around lipid droplets with perilipin, a regulator of lipolysis.

52 hus, we conclude that the central 25% of the perilipin A sequence contains all of the amino acids nec

53 Cells expressing perilipin A stored 6-30-fold more triacylglycerol than c

54 previously shown that the central region of perilipin A targets and anchors it to lipid droplets, at

55 imes slower in lipid-loaded cells expressing perilipin A than in lipid-loaded control cells, when tri

56 d droplets, while in cells stably expressing perilipin A, the lipid droplets were more numerous and t

57 To investigate perilipin function, perilipin A, the predominant isoform, was ectopically ex

58 amster ovary cells that express both HSL and perilipin A, these two proteins cooperate to produce a m

59 The mutation of serine 492 of perilipin A to alanine prevented the dispersion of clust

60 that the sequences responsible for anchoring perilipin A to lipid droplets are most likely domains of

61 To map the domains that target and anchor perilipin A to lipid droplets, we stably expressed fragm

62 the carboxyl terminus is required to target perilipin A to lipid droplets; however, there are multip

63 protein kinase A-mediated phosphorylation of perilipin A triggers the remodeling of lipid droplets.

64 otein levels of hormone-sensitive lipase and perilipin A, two proteins involved in adipocyte lipolysi

65 erminus of 112 amino acids that is unique to perilipin A were critical to facilitate triacylglycerol

66 boxyl-terminal truncation mutations of mouse perilipin A were stably expressed in 3T3-L1 preadipocyte

67 substitution of serines 433, 492, and 517 of perilipin A with glutamic acid residues blocked the disp