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1 he barrier imposed by the outer membrane was permeabilized).
2 less both cell walls and membranes have been permeabilized.
3 : (i) alive; (ii) fixed, and (iii) fixed and permeabilized.
4 he parasitophorous vacuole membrane (PVM) is permeabilized 10-30 min before its PKG-triggered breakdo
7 ications that utilize peptides with membrane-permeabilizing activities that are triggered by physiolo
9 AMP have at least a small amount of membrane-permeabilizing activity against multiple classes of micr
10 of this system, PopB and PopD, have membrane permeabilizing activity based on previous dye leakage ex
11 cent to the cationic sector induced a strong permeabilizing activity from liposomes composed of a mix
13 erformed a detailed analysis of the membrane permeabilizing activity of Bax and Bak at the single ves
14 hanism is still debated because the membrane permeabilizing activity of ceramide remains poorly under
15 ions, we tested the effects of EsxA membrane-permeabilizing activity on mycobacterial virulence and c
16 to mycobacterial virulence with its membrane-permeabilizing activity that is required for cytosolic t
17 However, evidence that link EsxA membrane-permeabilizing activity to Mtb cytosolic translocation a
18 on Q5K significantly diminished the membrane-permeabilizing activity, while Q5V enhanced the activity
20 R3/Nur77 is up-regulated by several vascular permeabilizing agents and has critical roles in mediatin
21 therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers
22 t-activating factor, small molecule vascular permeabilizing agents, also increased TR3/Nur77 expressi
23 factor (VPF), suggesting that other vascular permeabilizing agents, such as histamine and serotonin,
25 ponins, often show a superior performance to permeabilize and lyse membranes and/or a better suitabil
27 ficient actin staining was achieved in fixed permeabilized and non-permeabilized cells using differen
28 nocytochemistry was performed under both non-permeabilized and permeabilized conditions to detect sur
29 ntly reported that VEGF-A, a potent vascular permeabilizing and angiogenic factor, exerts much of its
32 sol transport of misfolded proteins in cells permeabilized at high cell density by perfringolysin O,
38 residing on the surface of lipid bilayer and permeabilizing bacterial membranes at high concentration
40 ith saponin (plasma membrane and ER membrane permeabilized), BeauIII inhibited SOAT1 (IC50, 1.8 micro
41 a treatment with digitonin (plasma membrane permeabilized), BeauIII selectively inhibited SOAT1 (IC5
47 using cells in which the plasma membrane was permeabilized, but the ciliary membrane was left intact.
55 re here the effect of stretch of contracting permeabilized cardiac trabeculae of the rat on the rate
57 , we compared SR Ca(2+) transport in saponin-permeabilized cardiomyocytes before and after limiting S
60 and contractile behaviour in single membrane-permeabilized cardiomyocytes in human left ventricular h
71 Considered together, these results establish permeabilized cells as a viable system in which to eluci
72 e complex (NPC) or the actin cytoskeleton in permeabilized cells demonstrated that the ciliary diffus
73 Only 4 of the 5 serum samples reacted to permeabilized cells enough to be distinguished from back
74 lear envelope (NE) was observed in digitonin-permeabilized cells in the absence of cytosolic factors.
75 (Na(3)VO(4)) to medium containing digitonin-permeabilized cells inhibits all ADP-ATP-using reactions
76 ADP-ATP exchange rate mediated by the ANT in permeabilized cells is measured for the entire range of
77 Mitochondrial Ca(2+) uptake was examined in permeabilized cells loaded with the mitochondria-selecti
80 interconvert adenine nucleotides such as in permeabilized cells that harbor phosphorylases and kinas
83 was achieved in fixed permeabilized and non-permeabilized cells using different combinations of dye
86 es mitochondrial calcium entry in intact and permeabilized cells, and attenuates the metabolic coupli
87 ake capacity of mitochondria, as measured in permeabilized cells, but correlates with an increase in
89 s focused primarily on import pathways using permeabilized cells, it has recently proven feasible to
90 ubiquitination of reductase in membranes of permeabilized cells, leading to enhanced dislocation of
91 enome by small molecular dyes in selectively permeabilized cells, nuclease protection, and flotation
93 lpha/CAS complex assembly and disassembly in permeabilized cells, using single-molecule fluorescence
94 3 of Fpn (anti-Fpn 172) detected Fpn only in permeabilized cells, whereas anti-Fpn 232 (amino acids 2
108 n vitro (isolated mitochondria) and in situ (permeabilized cells/tissue) approaches provide direct ac
110 stem by NaD1 for direct binding of PIP2 that permeabilizes cells via a novel membrane disrupting mech
113 derstanding the concerted action of membrane-permeabilizing compounds in biology as well as for optim
114 s performed under both non-permeabilized and permeabilized conditions to detect surface and intracell
115 ized with GAD on immunohistochemistry and in permeabilized cultured cerebellar GABAergic neurons, as
116 rapidly generated using endogenous iron when permeabilized Deltarnr4 spheroplasts are supplemented wi
117 maximal specific calcium-activated force of permeabilized diaphragm fibers were all significantly de
118 nly either a alpha-helical structure able to permeabilize dimyristoylphosphatidylcholine (DMPC) and d
120 e Ca(2+) release evoked by submaximal IP3 in permeabilized DKO1 and DKO2 but was ineffective in DKO3
123 ut heterogeneous tumor vasculature, with the permeabilizing effects of mild (40-42 degrees C) hyperth
124 e activity is membrane-disruptive in that it permeabilizes Escherichia coli and induces rapid microbi
125 permeability (AVH) induced by these vascular permeabilizing factors were greatly decreased in Nur77(-
127 oxygen consumption rate of left ventricular permeabilized fibers after 4 weeks of treatment was redu
130 MCU-mediated mitochondrial calcium uptake in permeabilized fibroblasts but not in isolated mitochondr
133 lerates the kinetics of force development in permeabilized heart muscle, but its role in vivo is unkn
134 tochondrial intermembrane space of digitonin-permeabilized hepatocytes, indicative of VDAC closure.
139 ers to elicit exocytosis when streptolysin O-permeabilized human sperm were loaded with inhibitory an
141 s fixed in situ; isolated rat cardiomyocytes permeabilized, incubated with 1 mmol/L Mg2+, and then fi
142 ty of exocytosis to Ca(2+) was documented in permeabilized INS-1 cells cultured in 11 versus 4 mm glu
143 optosis, the mitochondrial outer membrane is permeabilized, leading to the release of cytochrome c th
144 single 10 ns high-voltage electric pulse can permeabilize lipid vesicles and allow the delivery of si
145 The ability of these 14-mer peptides to permeabilize lipid vesicles therefore appears to be rela
148 more, adding purified cyclin C to unstressed permeabilized MEF cultures induced complete mitochondria
149 ng "nunchuck" CPPs, we demonstrate that CPPs permeabilize membranes by generating topologically activ
150 Certain antibiotic peptides are thought to permeabilize membranes of pathogens by effects that are
151 te the proapoptotic effectors BAX and BAK to permeabilize membranes without being universally sequest
153 f NaD1 has been attributed to its ability to permeabilize membranes; however, the molecular basis of
154 ant antimicrobial protein that opsonizes and permeabilizes membranes of microbial pathogens in mammal
157 0 known antimicrobial peptides (AMPs), which permeabilize microbial membranes but have diverse sequen
158 mediated by the ability of these peptides to permeabilize microbial membranes via the formation of am
159 a wide spectrum of antimicrobial activity by permeabilizing microbial membranes similar to other anti
161 TD-1, BTD-7), we show how these peptides all permeabilize model bacterial membranes but not model euk
162 urrent-voltage relationship of basolaterally permeabilized monolayers treated with Epac1 agonist 8-(4
163 showed that CLOOH-containing liposomes were permeabilized more readily by tBid plus Ca(2+) than CL-c
164 aling in situ, by analyzing Ca(2+) sparks in permeabilized mouse cardiomyocytes under a broad range o
165 ignificantly increased oxidative capacity in permeabilized muscle fibers (P-time x treatment < 0.05,
166 itochondrial OXPHOS capacity was measured in permeabilized muscle fibers by high-resolution respirome
168 Mitochondrial respiration was measured in permeabilized muscle fibers with high-resolution respiro
169 mal ADP-stimulated respiration was higher in permeabilized muscle fibers, which may contribute to the
170 of high-resolution respirometry performed in permeabilized muscle fibres prepared from muscle biopsie
171 ulated (State 3) respiration was measured in permeabilized muscle fibres using high-resolution respir
172 oteins increase force in partially activated permeabilized muscles, suggesting that they are sufficie
176 s determined by fluorescence measurements in permeabilized myocytes and isolated mitochondria), impai
177 endent fluorescent probe analyses in relaxed permeabilized myocytes corroborated these findings.
178 eitol reduced RyR-mediated SR Ca(2+) leak in permeabilized myocytes from old rabbit hearts to the lev
179 ng fluorescence resonance energy transfer in permeabilized myocytes, we specifically resolved RyR2-bo
182 cellular redox environment were performed in permeabilized myofibers and primary myotubes prepared fr
185 tial, abundance, and respiration assessed in permeabilized myofibers were not significantly altered i
186 fraction pattern analyses of single membrane-permeabilized myofibres showed, upon maximal Ca(2+) acti
188 tibody binding to live, fixed, and fixed and permeabilized neurons were observed, confirming that onl
189 ese inhibitors revealed intracellular PR3 in permeabilized neutrophils and on the surface of activate
196 rol the activity and selectivity of membrane-permeabilizing peptides, we screened a peptide library,
198 modulin-dependent protein kinase (CaMKII) in permeabilized phospholamban knockout (PLN-KO) mouse myoc
199 Moreover, sphingosine has been found to permeabilize phospholipid bilayers, giving rise to vesic
202 Previous studies using anti-syntaxin Abs in permeabilized platelets have suggested roles for both sy
203 cent dyes were released at higher rates from permeabilized polymersomes compared to unmodified ones.
204 For comparison, single myosins in relaxed permeabilized porcine papillary muscle fibers indicated
205 nts confirming that amphipathic and membrane-permeabilizing properties of CSA-13 are sufficient to di
207 is spectrin-disrupting fragment into a novel permeabilized proplatelet system rapidly destabilizes pr
210 domain of p63RhoGEF (residues 331-580) into permeabilized rabbit portal vein inhibited Ca2+ sensitiz
216 ion of various substrates and cofactors onto permeabilized rat gastrocnemius muscle fibers, as well a
219 Although alpha(v)beta(3) integrins regulate permeabilizing responses directed by vascular endothelia
220 t actively close the involved pathologically permeabilized retinal artery and normalize the vessel wa
221 Stiffness measurements in single membrane-permeabilized RV cardiomyocytes demonstrated a gradual i
223 -ray diffraction patterns of single membrane-permeabilized skeletal muscle fibers from mice lacking T
226 re, oxygen concentration, and in the case of permeabilized skeletal muscle, the contractile state of
227 n, belonging to a diverse family of membrane-permeabilizing small polypeptides involved in replicatio
228 TPgammaS)-loaded ARF6 (active form) added to permeabilized sperm induces acrosome exocytosis even in
229 used on cTnI as troponin can be exchanged in permeabilized striated muscle cell preparations, and tes
233 2, but not their respective amyloid fibrils, permeabilized synthetic bilayer membranes in vitro.
235 try experiments confirmed that both peptides permeabilize the bacterial cell membrane but suggested s
237 n emerging noninvasive method to temporarily permeabilize the BBB and the "blood-tumor barrier".
240 hibited a significantly stronger capacity to permeabilize the cytoplasmic membrane of Bacillus megate
241 nly well after growth halts does the peptide permeabilize the cytoplasmic membrane to GFP and the sma
242 r results demonstrate that it is possible to permeabilize the endothelial cells of the BBB in a contr
244 conformation changes to homooligomerize then permeabilize the mitochondrial outer membrane during apo
245 age or developmental signals, BAX and/or BAK permeabilize the mitochondrial outer membrane, resulting
247 enylarsonium and perfluorobutylsulfonate, to permeabilize the NPCs to naturally impermeable macromole
249 The pro-apoptotic Bcl-2 protein Bax can permeabilize the outer mitochondrial membrane and theref
250 icroneedles (MNs) selectively and painlessly permeabilize the outermost skin layer, allowing otherwis
251 ow concentration of digitonin to selectively permeabilize the plasma membrane of infected cells, we m
254 , or as cancer therapeutics that selectively permeabilize the plasma membranes of malignant cells.
255 conserved C-terminal delta peptide fragments permeabilize the plasma membranes of nucleated cells of
256 efore FISH and use xylenes and detergents to permeabilize the tissue rather than proteinase K, which
257 al barrier could both increase perfusion and permeabilize the tumor, enhancing chemotherapy penetrati
258 ool enriched in chain prenylated isoflavones permeabilized the bacterial membrane within minutes of e
261 antimicrobial peptide (CAMP) antibiotic that permeabilizes the bacterial outer membrane (OM) and has
262 However, if the peptide translocates, it permeabilizes the inner vesicles as well, which then sho
264 that mefloquine disrupts lysosomes, directly permeabilizes the lysosome membrane, and releases cathep
265 to most antimicrobial peptides, cWFW neither permeabilizes the membrane nor translocates to the cytop
266 t to the mode of action of OV-3 alone, which permeabilizes the membrane without lipid oxidation.
269 l to mitochondria, where it oligomerizes and permeabilizes the mitochondrial outer membrane to promot
270 When activated, the proapoptotic protein Bax permeabilizes the mitochondrial outer membrane, allowing
271 escent dye (carboxyfluorescein), the peptide permeabilizes the outer membrane, and dye enters the out
274 that chicken cathelicidin-2 kills E. coli by permeabilizing the bacterial inner membrane and subseque
277 e investigated whether ultrasound, which can permeabilize tissue through a phenomenon known as transi
278 sting that passive diffusion into previously permeabilized tissue is the major mechanism for the incr
279 e (temperature-jump of 0.5-20 masculineC) of permeabilized trabeculae over a physiological range of s
280 IP(3) was also able to release Ca(2+) from permeabilized trypanosomes or isolated acidocalcisomes a
281 ochondrial Ca(2+) accumulation in intact and permeabilized trypanosomes, generates excessive mitochon
284 oscillations of circulating microbubbles to permeabilize vascular barriers such as the blood-brain b
285 osphatase phosphorylation in both intact and permeabilized vascular, gut, and airway SMs independentl
286 R2 channels in bilayers and Ca(2+) sparks in permeabilized ventricular cardiomyocytes is defined.
287 Ca(2+) flux amplitude and SR Ca(2+) load in permeabilized ventricular myocytes as an endogenous cell
288 reticulum (SR) Ca(2+) dynamics in intact and permeabilized ventricular myocytes using confocal micros
292 hibits the ability of preformed oligomers to permeabilize vesicles and induce cytotoxicity in a rat b
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