ライフサイエンスコーパス: pgRNA

1 pgRNA is a terminally redundant transcript whose synthes

2 pgRNA is reverse transcribed by the viral polymerase int

3 pgRNA is then reverse transcribed to double-stranded DNA

4 udies of capsid protein, DNA polymerase, and pgRNA between HBV and WHV suggest that HBV capsid protei

5 wn to be dispensable for protein priming and pgRNA binding, respectively.

6 sequence requirements for Hepsilon binding, pgRNA packaging, and protein priming allowed the classif

7 of the 3' region because isolation of capsid pgRNA by an alternative method that did not involve nucl

8 experiments show that the 3' ends of capsid pgRNA isolated by micrococcal nuclease treatment are het

9 Analysis of capsid pgRNA isolated by using an established method involving

10 de evidence that the 3' region of the capsid pgRNA has biochemical properties different from those of

11 ts indicate that the 3' region of the capsid pgRNA is susceptible to micrococcal nuclease digestion d

12 on can still be underrepresented when capsid pgRNA is isolated without nuclease digestion.

13 ions; "immature" NCs, i.e., those containing pgRNA or immature reverse transcription intermediates, a

14 between the various viral components during pgRNA encapsidation.

15 es (nt) 438 and 720 contributed to efficient pgRNA encapsidation, while the sequence between nt 538 a

16 Full protection of encapsidated pgRNA from nuclease was observed for HBcAg 1-171.

17 tervening sequence and region II facilitates pgRNA encapsidation.

18 capsid assembly although it is essential for pgRNA packaging.

19 and the capsid subunit (C) are necessary for pgRNA encapsidation.

20 Subsequently, the TP domain is necessary for pgRNA packaging into viral nucleocapsids and the initiat

21 nown requirement of core phosphorylation for pgRNA packaging and DNA synthesis, suggest that the NC u

22 Crucial residues for pgRNA packaging are contained within aa 141 to 160, indi

23 t core protein has intrinsic specificity for pgRNA, independent of the polymerase.

24 raction was necessary but not sufficient for pgRNA packaging.

25 virus (DHBV), a spliced RNA is derived from pgRNA by removal of a single intron.

26 Inhibition of Hsp90 led to diminished HBV pgRNA packaging into nucleocapsids in cells, which depen

27 ally via accelerating the degradation of HBV pgRNA independent of La protein.

28 s not involved in the polyadenylation of HBV pgRNA.

29 170-nucleotide region from the 5' end of HBV pgRNA; a large portion of this region is duplicated at t

30 iophysical chemistry of Cp caused defects in pgRNA packaging and synthesis of the second strand of DN

31 er, all of these mutations led to defects in pgRNA packaging.

32 her viral components in order to function in pgRNA encapsidation.

33 enotype G with genotype A sequence increased pgRNA transcription and genome replication, implicating

34 one encapsidated a nuclease-sensitive 3.5-kb pgRNA while the other encapsidated a nuclease-resistant

35 he IRSE, as IRSE-mutant HBV transcribed less pgRNA and could not be repressed by IFN-alpha treatment.

36 has a low replication capacity due to a low pgRNA level.

37 cludes the splicing machinery from modifying pgRNA.

38 Neither Cp nor pgRNA localized to the mitochondria during active replic

39 cient for capsid assembly, in the absence of pgRNA or any other viral or host factors, under conditio

40 ted base pairing reduced the accumulation of pgRNA and increased the accumulation of spliced RNA.

41 e activity of HBV enhancer and the amount of pgRNA transcribed from cccDNA) were significantly higher

42 r events that either inhibit the assembly of pgRNA-containing capsids or accelerate their degradation

43 One region is near the 5' end of pgRNA (region A), while the second is near the middle of

44 silon to an acceptor site near the 3' end of pgRNA and synthesis of a complete minus-strand DNA.

45 dation: epsilon, which is near the 5' end of pgRNA, and region II, located near the middle of pgRNA.

46 sidation signal (epsilon) near the 5' end of pgRNA; and (ii) a template switch of the four-nucleotide

47 ions of the DHBV genome reduced the level of pgRNA while increasing the level of spliced RNA.

48 n A), while the second is near the middle of pgRNA (region B).

49 A, and region II, located near the middle of pgRNA.

50 which is required for specific packaging of pgRNA into viral nucleocapsids and initiation of viral r

51 which is required for specific packaging of pgRNA into viral nucleocapsids and initiation of viral r

52 contained within the terminal redundancy of pgRNA, and the 5' copy of this sequence is essential for

53 ysical basis for electrostatic regulation of pgRNA packaging in HBV by using a coarse-grained molecul

54 tant in sequence, resulted in restoration of pgRNA accumulation with a decrease in the level of splic

55 We propose that this topology of pgRNA facilitates first-strand template switch and/or th

56 ignal, termed epsilon (Hepsilon), located on pgRNA, which is required for specific packaging of pgRNA

57 signal termed epsilon (Hepsilon) located on pgRNA, which is required for specific packaging of pgRNA

58 ) indicated that two cis-acting sequences on pgRNA are required for encapsidation: epsilon, which is

59 elf as a protein primer and an RNA signal on pgRNA, termed epsilon (Hepsilon), as the obligatory temp

60 ed in order to study the replication of only pgRNA.

61 s reverse transcription of an RNA pregenome (pgRNA) by a multifunctional polymerase (HP).

62 t is based on a lentiviral paired-guide RNA (pgRNA) library.

63 addition to packaging viral pregenomic RNA (pgRNA) and DNA polymerase complex into nucleocapsids for

64 rmed to measure encapsidated pregenomic RNA (pgRNA) and minus-strand DNA synthesized in cell culture.

65 tein (Cp) packages the viral pregenomic RNA (pgRNA) and polymerase to form the HBV core.

66 reasing the transcription of pregenomic RNA (pgRNA) and subgenomic RNA from the HBV covalently closed

67 ly encapsidates a complex of pregenomic RNA (pgRNA) and viral polymerase; it has been suggested that

68 ns (Cps) assemble around the pregenomic RNA (pgRNA) and viral reverse transcriptase (P).

69 ging signal, epsilon) on the pregenomic RNA (pgRNA) by the viral reverse transcriptase (RT).

70 h reverse transcription of a pregenomic RNA (pgRNA) by using a multifunctional polymerase (HP).

71 h reverse transcription of a pregenomic RNA (pgRNA) by using a multifunctional polymerase (HP).

72 s of hepatitis B virus (HBV) pregenomic RNA (pgRNA) harbors sites governing many essential functions

73 (Cp), polymerase (Pol), and pregenomic RNA (pgRNA) in infected cells.

74 Packaging of hepadnavirus pregenomic RNA (pgRNA) into capsids, or encapsidation, requires several

75 begins with packaging of the pregenomic RNA (pgRNA) into immature nucleocapsids (NC), which are conve

76 cis-acting sequences on the pregenomic RNA (pgRNA) involved in the synthesis of minus-strand DNA.

77 The pregenomic RNA (pgRNA) of hepadnaviruses is packaged into capsids where

78 The pregenomic RNA (pgRNA) of hepatitis B virus (HBV) serves as the messenge

79 ction with those for in vivo pregenomic RNA (pgRNA) packaging clearly indicated that RT-epsilon inter

80 Pregenomic RNA (pgRNA) plays two major roles in the hepadnavirus life cy

81 owed that HNF6 reduced viral pregenomic RNA (pgRNA) posttranscriptionally via accelerating the degrad

82 opies in and adjacent to the pregenomic RNA (pgRNA) terminal redundancy, that were specifically recog

83 ficient encapsidation of its pregenomic RNA (pgRNA), epsilon and region II.

84 In addition to the 3.5-kb pregenomic RNA (pgRNA), the mutant preferentially encapsidated the 2.2-k

85 the pool of cytoplasmic HBV pregenomic RNA (pgRNA)-containing capsids is reduced 10-fold within 9 h

86 tly inhibit the formation of pregenomic RNA (pgRNA)-containing nucleocapsids of HBV but not other ani

87 a reverse transcription of a pregenomic RNA (pgRNA).

88 ntribute to encapsidation of pregenomic RNA (pgRNA).

89 f an RNA intermediate termed pregenomic RNA (pgRNA).

90 fically binding to the viral pregenomic RNA (pgRNA).

91 psid that packages the viral pregenomic RNA (pgRNA).

92 e (C) ORF on the bicistronic pregenomic RNA (pgRNA).

93 ugh an RNA intermediate (the pregenomic RNA, pgRNA) by reverse transcription.

94 Mutagenesis experiments suggest that pgRNA encapsidation hinges on its strong electrostatic i

95 erse transcription within NCs converting the pgRNA into the characteristic dsDNA genome.

96 HBV replication begins with packaging of the pgRNA and P protein into core protein particles, followe

97 rated reduced levels of the 3' region of the pgRNA compared to the 5' region.

98 We found that large portions of the pgRNA could be deleted or substituted without an appreci

99 % of scanning ribosomes at the 5' end of the pgRNA greatly inhibited C and P synthesis.

100 al assembly begins with the packaging of the pgRNA into nucleocapsids (NCs), with subsequent reverse

101 DNA synthesized, indicating that most of the pgRNA is dispensable and that a specific size of the pgR

102 Possibly, the 3' region of the pgRNA is not packaged into the interior of the capsid bu

103 dispensable and that a specific size of the pgRNA is not required for this process.

104 istent with a model in which splicing of the pgRNA is suppressed by a secondary structure between reg

105 tween the 3' region and other regions of the pgRNA isolated from capsids.

106 sequence, which is located downstream of the pgRNA polyadenylation site, overlaps the core (C) protei

107 d from a donor region near the 5' end of the pgRNA to an acceptor site at or near the P AUG, and the

108 n and region II disrupted the ability of the pgRNA to be encapsidated efficiently.

109 reverse transcription for conversion of the pgRNA to viral DNA.

110 Translation of the pgRNA was found to be cap dependent, because inserting a

111 lon, an RNA stem-loop near the 5' end of the pgRNA, has been characterized in detail, while region II

112 hile region II, located in the middle of the pgRNA, is not as well defined.

113 ing sequences present near the 3' end of the pgRNA, was introduced at that location.

114 A are present near the 5' and 3' ends of the pgRNA.

115 ed C and P translation in the context of the pgRNA.

116 ly studied function of Pol is to package the pgRNA and reverse transcribe it to double-stranded DNA w

117 Our high-throughput pgRNA genome deletion method will enable rapid identific

118 various viral components that contribute to pgRNA encapsidation.

119 TD increase the proportion of spliced RNA to pgRNA that are encapsidated and reverse transcribed.

120 neous cytoplasmic accumulation of unspliced (pgRNA) and spliced RNA was not known.