ライフサイエンスコーパス: phage T4

1 quences were displayed on the HOC protein of phage T4.

2 vI, the other homing endonuclease encoded by phage T4.

3 hat have been invoked for intron mobility in phage T4.

4 and bacteriophages, including the dCTPase of phage T4.

5 cific regions of extensive conservation with phage T4.

6 in vitro and in vivo, compared with that of phage T4.

7 omain--a second RNA ligase (Rnl2) encoded by phage T4.

8 homologous TS gene (td) of Escherichia coli phage T4.

9 of single DNA molecule packaging dynamics in phage T4, a large, tailed Escherichia coli virus that is

10 a pattern similar to what has been noted in phage T4 and its relatives, in which there is minimal su

11 In Escherichia coli phage T4 and many of its phylogenetic relatives, gene 43

12 teria, introns have been encountered only in phage T4 and several of its close relatives.

13 ich includes the introns in Escherichia coli phage T4 and the Bacillus phages beta22 and SPO1.

14 e with those found in the gene 61 primase of phage T4 and the DnaG primase of Escherichia coli.

15 ination factor Rho, that block the growth of phages T4 and lambdar32.

16 e DNA polymerases (gp43s) of the two related phages T4 and RB69 are DNA-binding proteins that also fu

17 ent (KF) and two other DNA polymerases, from phages T4 and T7.

18 ral recombination is essential for growth of phage T4, because origin initiation of DNA replication i

19 uous packaged DNA structures are favored for phage T4 by a number of lines of evidence.

20 The data showed that phage T4 can successfully package and transduce 4 to 29

21 Unlike most phages, T4 can sense superinfection (which signals the d

22 The phage T4 capsid lattice provides a stable biological pla

23 and an engineered gp41 C-peptide trimer, on phage T4 capsid surface through Hoc-capsid interactions.

24 anthrax toxin molecules are assembled on the phage T4 capsid under controlled conditions.

25 6-adenine]-methyltransferase (Dam MTase) of phage T4 catalyzes methyl group transfer from S-adenosyl

26 sed on the crystallographic structure of its phage T4 counterpart.

27 ly revealed its sequence relationship to the phage T4 dCTPase, phosphoribosyl-ATP pyrophosphatase His

28 In phage T4 development the gene 59 protein (gp59) assemble

29 Mature phage T4 DNA and linearized plasmid DNAs containing or l

30 ides were used as templates for synthesis by phage T4 DNA polymerase holoenzyme proficient or deficie

31 uble-stranded DNA unwinding catalyzed by the phage T4 DNA replication helicase (gp41).

32 cesses occurring during the formation of the phage T4 DNA sliding clamp, we have performed direct sub

33 Phage T4 effects lysis by its holin T and its endolysin

34 We have identified the phage T4-encoded sigma factor gp55 and the co-activator

35 Phage T4 endonuclease VII (endo VII) was the first enzym

36 Like most phages with double-stranded DNA, phage T4 exits the infected host cell by a lytic process

37 protein, sCARfC6.5, that consisted of sCAR, phage T4 fibritin polypeptide, and C6.5 single-chain fra

38 different proteins: the Ad serotype 5 fiber, phage T4 fibritin, and the human CD40 ligand (CD40L).

39 Phage T4 gene 32 protein (gp32) is a zinc metalloprotein

40 ammed ribosomal bypassing occurs in decoding phage T4 gene 60 mRNA.

41 Half the ribosomes translating the mRNA for phage T4 gene 60 topoisomerase subunit bypass a 50 nucle

42 ncoded by Escherichia coli (SSB protein) and phage T4 (gene 32 protein) also have acidic COOH-termina

43 progeny of mixed infections with the related phage T4 (general, or phage exclusion).

44 terminases and bear great similarity to the phage T4 gp17 but are distinct from podovirus and siphov

45 e report single-molecule measurements of the phage T4 gp17 motor by using dual-trap optical tweezers

46 The phage T4 gp45 sliding clamp is a ring-shaped replication

47 nisms such as Escherichia coli, phage T7 and phage T4 has demonstrated the essential nature of primas

48 The tail of phage T4 has long served as the paradigm for understandi

49 Phage T4 has many optional homing endonuclease genes sim

50 Phage T4 has three group I introns, within the td, nrdB

51 Escherichia coli and selective phages T4 have been used as case study.

52 Here, the catalytic centre of phage T4 headful nuclease, present in the C-terminal dom

53 of the interaction was tested by means of a phage T4 HOC (highly antigenicoutercapsid protein) displ

54 The data suggest that the phage T4 hoc-soc system is an attractive system for disp

55 A new phage T4 in vitro DNA packaging assay employed purified

56 The end-healing and end-sealing steps of the phage T4-induced RNA restriction-repair pathway are perf

57 (EcoPrrC) underlies an antiviral response to phage T4 infection.

58 ccumulate in prohead and terminase defective phage T4 infections could be packaged in vitro to approx

59 The DNA-binding DNA polymerase (gp43) of phage T4 is also an RNA-binding protein that represses t

60 ve bacteria and AQUIFEX: Endonuclease VII of phage T4 is shown to serve as a structural template for

61 and "join-cut-copy," can be distinguished in phage T4: join-copy requires only early and middle genes

62 gnition and ATP hydrolysis in the pentameric phage T4 large "terminase" (gp17) motor.

63 We have probed the mechanism of the phage T4 large terminase subunit gp17 by analyzing linea

64 gp55-dependent phage T4 late promoter transcription is also resistant t

65 lease HI (RNase H) from Escherichia coli and phage T4 lysozyme (T4L) both exhibit such a phenomenon.

66 lease HI from Escherichia coli (RNase H) and phage T4 lysozyme (T4L) reveal that, for both proteins,

67 The complexes of phage T4 lysozyme L99A with noble gases have been studie

68 solution with a number of cavity containing phage T4 lysozyme mutants show that xenon can report on

69 ned 434 cro variants, as well as a series of phage T4 lysozyme variants.

70 e A2, proteinase A, rubredoxin, thrombin and phage T4 lysozyme) and appear with similar coordination

71 This strategy was tested on phage T4 lysozyme, a protein whose crystallization as a

72 and another that is structurally similar to phage T4 lysozyme.

73 gram ORBIT, was used to redesign the core of phage T4 lysozyme.

74 seudo wild-type and cavity-containing mutant phage T4 lysozymes in the presence of argon, krypton, an

75 The availability of a series of phage T4 lysozymes with up to 14 methionine residues inc

76 The phage T4 motor is composed of a dodecameric portal and s

77 The phage T4 motor is composed of the small terminase protei

78 The phage T4 motor is constituted by gene product 16 (gp16)

79 sity twice that of an automobile engine, the phage T4 motor is the fastest and most powerful reported

80 The phage T4 motor, a pentamer of 70-kDa large terminase, gp

81 he new approach is based on using label-free phages (T4), obligate parasites of bacteria, which are a

82 Intron homing in phage T4 occurs in the context of recombination-dependen

83 ely 2,000 bp/s, consistent with packaging by phage T4 of an enormous, 171-kb genome in <10 min during

84 These results suggest that the phage T4 packaging machine consists of a motor (gp17) an

85 The phage T4 packaging machine consists of three components:

86 id packaging results show that initiation of phage T4 packaging on "endless" concatemeric DNA in vivo

87 g experiments, we observed that the in vitro phage T4 packaging system can package and transduce DNA

88 A defined phage T4 packaging system consisting of only two compone

89 A bipartite phage T4 peptide library was created by displaying on te

90 DNA polymerase (gp43) of phage T4 plays two biological roles, one as an essential

91 Structural analysis of phage T4 portal (gp20) has been hampered because of its

92 Phage T4 protects its DNA from the two-gene-encoded gmrS

93 s (equivalent to the 11 residues of 43P from phage T4) protrude from the thumb domain and are free to

94 e two-dimensional gel analysis also revealed phage T4 replication intermediates not previously detect

95 ication structures generated by the in vitro phage T4 replication system were analyzed using two-dime

96 ted sliding-clamp processivity factor of the phage T4 replisome (gp45, the activator).

97 Loading of the phage T4 sliding clamp gp45 by the gp44/62 clamp loader

98 First, optimization experiments with phage T4 spiked in complex matrices (without a phage amp

99 , the ribosome bypass (or "hop") sequence of phage T4 stands out as a uniquely extreme example of pro

100 The phage T4 system offers new direction and insights for HI

101 DNA polymerase of phage T4 (T4 gp43), an essential component of the T4 DNA

102 ome were inserted upstream of a promoterless phage T4 td gene, and fragments that led to complementat

103 contrasts with efficient inheritance of the phage T4 td group I intron and its endonuclease, I-TevI,

104 The phage T4 td intron endonuclease, I-TevI, is responsible

105 Homing of the phage T4 td intron is initiated by the intron-encoded en

106 Escherichia coli genetic assay based on the phage T4 td intron to systematically test the ability of

107 Escherichia coli genetic assay based on the phage T4 td intron to test the ability of the Neurospora

108 I-TevI, the phage T4 td intron-encoded endonuclease, recognizes a le

109 ent and stabilization of functional sites in phage T4 terminase and other double-stranded DNA phage t

110 ET has shown a decrease in distance from the phage T4 terminase C terminus to portal consistent with

111 Mutations in the phage T4 terminase C-motif lead to loss of stimulated AT

112 Phage T4 terminase is a two-subunit enzyme that binds to

113 Phage T4 terminase is an enzyme that binds to the portal

114 bipartite library was biopanned against the phage T4 terminase large subunit gp17 to identify T4 gen

115 We have constructed new phage T4 terminase recombinants under the control of pha

116 packaged into empty viral procapsids by the phage T4 terminase with high efficiency in vitro.

117 t that mutations of basic residues that line phage T4 TerS (gp16) channel do not disrupt DNA binding.

118 G family endonuclease adjacent to gene 32 of phage T4 that is absent from phage T2.

119 For phage T4, the most obvious change is the contraction of

120 In phage T4, the pseudoknot is contained within 28 contiguo

121 In phage T4, there is evidence that the large terminase pro

122 In phage T4, there is evidence that this vertex, constitute

123 In phage T4 they are genes 16 and 17.

124 In phage T4 this complex consists of a trimeric clamp of gp

125 g capabilities that were observed, may allow phage T4 to coordinate DNA packaging with other ongoing

126 vsY, 46 and 59) increased the sensitivity of phage T4 to m-AMSA, strongly suggesting that recombinati

127 f RNase BN is not required for maturation of phage T4 tRNA precursors, a known specific function of t

128 Phage T4, unlike the phages with defined ends (e.g. lamb

129 The phage T4 UvsW protein has been shown to play a crucial r

130 hat targets the thymidylate synthase gene of phage T4, we readily isolated variants that dramatically

131 x-site terminated plasmid DNAs injected from phage T4 were recircularized by T4 ligase in vivo with a