ライフサイエンスコーパス: phage display

1 ved from combinatorial experiments (SELEX or phage display).

2 internalized by cells, designated z13, using phage display.

3 ctivities by expressing Evasin mutants using phage display.

4 ted TUPS among peptide sequences selected by phage display.

5 scovery of new peptides and proteins through phage display.

6 ression markers or peptides discovered using phage display.

7 phopeptide binding specificities in vitro by phage display.

8 E inhibitory human antibody using "two-step" phage display.

9 ecting variants with improved affinity using phage display.

10 immunoreagents are generated using antibody-phage display.

11 irs of recombinant affinity reagents through phage-display.

12 Three phage-displayed 9-mer disulfide-constrained peptides tha

13 Here, using phage display affinity maturation, we developed a high-a

14 selected broadly neutralizing nanobodies by phage display after immunization of dromedaries with dif

15 d from a minimalist synthetic library during phage display against a branched RNA target.

16 Phage display against D-VEGF-A was used to screen design

17 antibodies generated from large libraries by phage display against important human antigen targets, w

18 Here we used phage display against virus-like particles (VLPs) to iso

19 Phage display allows material scientists to design speci

20 M13 phage displaying an in vivo biotinylatable peptide (AviT

21 oach for generation of such antibodies using phage display and affinity maturation.

22 on-antibody binding proteins against GPC3 by phage display and developed a new sandwich chemiluminesc

23 Here we describe a system that combines phage display and efficient mammalian expression in a si

24 ral monoclonal antibodies were isolated from phage display and hybridoma platforms by functional scre

25 roteome of pancreatic cancer endothelium via phage display and identify hornerin as a critical protei

26 designed using structure-guided mirror-image phage display and linker optimization and is the first D

27 Monoclonal antibody technologies, phage display and mRNA display, are methods that could b

28 Aided by mirror image phage display and native chemical ligation, we have prev

29 We employed substrate phage display and positional proteomics to compare subst

30 es are now the most widely used vehicles for phage display and provide efficient means for epitope id

31 receptor ectodomain have been discovered by phage display and reported in the literature.

32 icular Ub-specific proteases (USPs) and used phage display and saturation scanning mutagenesis to com

33 This study suggests that phage display and selection with other MMPs may be an ef

34 methods such as structure-based drug design, phage display and surface science.

35 from a large synthetic antibody library with phage display and used to develop a single-step sandwich

36 protein libraries, screened them in vitro by phage display, and analyzed their response to selection

37 By combining genetic immunization, phage display, and biopanning, we identified two functio

38 ibe the use of computational protein design, phage display, and high-throughput binding assays to cre

39 HS-specific phage display antibodies (HS3A8 and RB4EA12) showed sign

40 Using a panel of phage display antibodies, we confirmed that these mutati

41 ration of a recombinant antibody library and phage-displayed antibodies.

42 ndependently of the surrounding scaffold, as phage display antibody libraries using these scaffolds y

43 oth HT-2 and T-2 toxins was developed from a phage display antibody library containing 6 x 10(7) diff

44 glycoprotein complex E1E2 from an HCV-immune phage-display antibody library by using an exhaustive-pa

45 the anti-Dsg3 IgG(+) repertoire by antibody phage display (APD) and PCR indicated that six clonal li

46 Numerous examples of phage display applied to soluble proteins demonstrate th

47 Here, using in vivo phage display approach and the partial carotid ligation

48 To improve binding affinity, we used a phage display approach by randomizing seven PCSK9 contac

49 We describe a phage display approach that we have previously used to g

50 We used a phage display approach to identify synthetic antibodies

51 A phage display approach was herein used to select motifs

52 tumor penetrating peptides identified using phage display are not known.

53 Affinity reagents that are generated by phage display are typically subcloned into an expression

54 Through phage display-based functional proteomics, immunohistoch

55 Phage display biopanning identified Gly-Ile-Arg-Leu-Arg-

56 tegration of enzymatic processing steps into phage display biopanning to expand the biocombinatorial

57 From an initial phage display biopanning, a series of peptide ligands fo

58 enerate M. bovis-specific peptide ligands by phage display biopanning.

59 that high-throughput sequencing can empower phage display by (i) enabling the analysis of complex bi

60 e we show that bicyclic peptides isolated by phage display can target the E2 binding sites on the HEC

61 a high-throughput method, we developed a T7 phage display cDNA library derived from mRNA isolated fr

62 In brief, random peptides were encoded by phage display, chemically cyclized with an azobenzene li

63 Using phage display combined to rational design, we developed

64 Here we demonstrate the use of lambda-phage displaying Cry1Aa13 toxin variants modified in dom

65 set, we use a considerable amount of recent phage display data that describe the peptide recognition

66 es that have structural information but lack phage display data.

67 croarray data and shows utility in analyzing phage display datasets.

68 Phage display empowered the development of proteins with

69 We have developed a phage display engineering strategy to generate synthetic

70 and MCP are ideally positioned for DGR-based phage-display engineering.

71 s of WWOX, we employed mass spectrometry and phage display experiments to identify putative WWOX-inte

72 oviding suitable starting points for seeding phage display experiments.

73 ic rabbit/human anti-Rev Fab was selected by phage display, expressed in a bacterial secretion system

74 entified from an MAA-enriched umbilical cord phage displayed Fab library, and a derived Fab with the

75 ement of metastatic prostate cancer, we used phage display fingerprinting to analyze sequentially acq

76 s, alternative methods such as comprehensive phage display, fluid-phase immunoassays, and antigen mic

77 n after three and six rounds of selection by phage display for binding to its peptide ligand.

78 hetic antibody-fragment (Fab) library in the phage-display format and isolated antibody-fragments tha

79 Phage display frequently identified recognition preferen

80 we characterized Dsg-specific mAbs cloned by phage display from 3 patients with pemphigus vulgaris an

81 ecificity and affinity, were retrieved after phage display from a large 'immune' library constructed

82 t-based discovery (GE-FBD) uses selection of phage-displayed glycopeptides to dock a glycan fragment

83 led nerve-binding peptide, NP41, selected by phage display, highlights peripheral nerves in vivo.

84 By using phage display in combination with the controlled deposit

85 es to random library screening methods (e.g. phage display), in vitro cellular-based experiments and

86 Phage display is a key technology for the identification

87 Phage display is a powerful method for identifying pepti

88 Phage display is a powerful method for selecting affinit

89 Phage display is a powerful technology that selects spec

90 Phage display is a prominent screening technique with a

91 The success of phage display is due to its robustness, ease of use, and

92 Phage display is used to discover peptides or proteins w

93 tide macrocycle that was recently evolved by phage display (Ki = 0.84 +/- 0.03 nM).

94 ers, we used H1N1pdm09 whole-genome-fragment phage display libraries (GFPDL) to evaluate the antibody

95 H5N1 whole-genome fragment phage display libraries (GFPDL) were used to elucidate t

96 Here we used whole-genome gene fragment phage display libraries (GFPDLs) expressing peptides of

97 ivors in Vietnam using whole-genome-fragment phage display libraries (GFPDLs).

98 ogether these data suggest that selection of phage display libraries against a clonal progenitor stem

99 Peptides were selected from 7- to 15mer phage display libraries by panning with hyaluronan-Sepha

100 hat peptides obtained from the biopanning of phage display libraries can be readily used as sensing p

101 Antibody phage display libraries combined with high-throughput se

102 Two 15 amino acid peptides selected from phage display libraries demonstrated a high affinity (av

103 mmunized mice with ACT and screened antibody phage display libraries for binding to purified ACT.

104 e-borne peptidomimetics can be selected from phage display libraries in a straightforward systematic

105 Screening phage display libraries is one approach to detecting suc

106 Starting from large phage display libraries of single-chain antibody fragmen

107 port the engineering and characterization of phage display libraries of stable human VH domains and t

108 tides of 7 to 12 amino acids identified from phage display libraries using both bioinformatics-based

109 Whole genome-fragment phage display libraries, expressing linear and conformat

110 nding of Salmonella to peptides derived from phage display libraries.

111 nst different conformations of betaarrs from phage display libraries.

112 ies have been detected by screening antibody phage display libraries.

113 n-domain monobodies originally selected from phage-display libraries.

114 we use next-generation sequencing to analyze phage-displayed libraries and uncover a strong bias indu

115 Using G2 as a scaffold, we constructed phage-displayed libraries of FHA1 variants and affinity

116 selection of the scaffold surface to vary in phage display, libraries can be designed that present se

117 -exposed individuals by using a whole-genome phage display library (H7N7-GFPDL) to explore the comple

118 he complete human proteome, the T7 peptidome phage display library (T7-Pep), and demonstrate its appl

119 We have generated an immunized phage display library against ApoB-100 and isolated four

120 Here we describe the construction of a VHH phage display library against the cyanobacterial hepatot

121 sequence space of a given scaffold through a phage display library and by (ii) panning multiple libra

122 and other species was isolated from a human phage display library and engineered to contain an IgG1

123 antibodies was generated by selection from a phage display library and extensively analyzed in vitro.

124 adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and

125 A mutagenized substrate phage display library based on a 73-amino acid fragment

126 Fabs were recovered from a combinatorial Fab phage display library constructed from bone marrow-deriv

127 cDNA, and cloned into a phagemid vector for phage display library construction.

128 A phage display library covering 10(9) unique dodecapeptid

129 rabbit antibody repertoire represented by a phage display library encompassing >10 billion independe

130 ting agent to screen a randomized 12-residue phage display library for peptides that bind strongly to

131 We created a phage display library from llamas immunized with ricin t

132 A 9-mer pVIII M13 phage display library is screened against U937 to identi

133 vel technique by screening fibrinogen with a phage display library of 3 billion random, conformationa

134 Here, we describe the first fully synthetic phage display library of humanized llama single domain a

135 A phage display library representing a highly diverse arra

136 Using a phage display library screen, we identified a peptide, P

137 Here we report results from a random M13-phage display library screening to isolate 12-mer peptid

138 was identified by both Peptide Scanning and Phage Display Library screening, other approaches, such

139 in antibody (nanobody) isolated from a llama phage display library that confers potent neutralizing c

140 cells were used in a screen of a human scFv phage display library that included CDR3 engineered to o

141 We have utilized a high-diversity phage display library to engineer antibody fragments (Fa

142 MT-SP1 (membrane-type serine protease 1)], a phage display library was created with a natural reperto

143 specific binders were generated using a Fab phage display library with CBP-fused constructs.

144 Screening of a phage display library with CR as bait revealed a highly

145 faces facing the peritoneum, we subtracted a phage display library with female mouse peritoneum tissu

146 e identified through immunocreenings of a T7 phage display library with high accuracy, which may have

147 r three rounds of biopanning by 1E4 from the phage display library, a mimetic peptide, m1E41920, was

148 cterized HAIYPRH, from the M13-based Ph.D.-7 phage display library, as a propagation-related TUP resu

149 Using a combinatorial histidine-scanning phage display library, potential metal binding sites wer

150 By screening a peptide phage display library, we discovered a novel ligand (PDN

151 human fragment of the antigen binding (Fab) phage display library, we identified 12 unique human Fab

152 Using a human fragment antigen-binding phage display library, we identified a human antibody te

153 Using a phage display library, we identified an anti-PCSK9 Fab (

154 By using phage display library, we identified two highly specific

155 By screening a phage display library, we previously identified a peptid

156 ning of a naive llama single-domain antibody phage display library.

157 , TPFDLRPSSDTR, which is identified by using phage display library.

158 nerated, characterized, and used to screen a phage display library.

159 FVIII-specific scFv derived from a synthetic phage display library.

160 teome can be represented by a random peptide phage display library.

161 nI), previously identified from a polyvalent phage displayed library, has been immobilized on a gold

162 pertoires were elucidated by genome-fragment phage-display library analysis, and antibody avidities f

163 A phage-display library consisting of the VH H region cont

164 variable fragments (Fvs), and constructed a phage-display library containing Fvs that bind to the RI

165 We screened a phage-display library expressing the entire complement o

166 We isolated four distinct nanobodies from a phage-display library generated from an alpaca immunized

167 Reaction between M13 phage-displayed library of peptides terminated with an a

168 , Staquicini and colleagues apply an in vivo phage-displayed library of random peptides to identify d

169 Such phage-displayed ligands offer useful reagents for biosen

170 cific progenitor cell-binding peptides using phage display may be hindered by the large cellular hete

171 The results also suggest that phage display may offer a useful approach for rapid inve

172 for the infarct/border zone, we used in vivo phage display methods and an optical imaging approach: f

173 various size (15-450 nm) using combinatorial phage display methods.

174 d control samples confirmed a major issue in phage display, namely the selection of unspecific peptid

175 Here, we used next generation phage-display (NGPD) and a 2-proportion Z score analysis

176 a1 strand was discovered to be essential for phage display of a functional FHA1 domain.

177 ve mutants of the I domain, and screening of phage display of human antibody library against the acti

178 ith native proteins and for the selection by phage display of in vivo-matured Nanobodies that bind co

179 g and large-scale mutagenesis enabled by the phage display of MPs.

180 Therefore, we report data from the phage display of representative types of membrane-associ

181 Phage display offers a powerful approach to engineer pro

182 ning antibody variable domains, generated by phage display or derived from human/humanized monoclonal

183 preserves their individual identities (e.g., phage display or one-bead one-compound).

184 performed myeloma cell surface screenings of phage-displayed patient transplant immunomes.

185 Phage display (PD) is frequently used to discover peptid

186 We discuss the contribution of phage display peptide libraries in determining dominant

187 g an aggregated mAb as bait for screening of phage display peptide library and identifying those pept

188 hat peptides identified from a combinatorial phage display peptide library assemble preferentially to

189 Previously, a phage display peptide library screen identified SM1, a p

190 Epitope mapping, using a phage display peptide library, revealed that cAb29 binds

191 of human leukemia cells with a combinatorial phage display peptide library, we isolated a peptide mot

192 ch is based on a unique mix of comprehensive phage displayed peptide screening processes, along with

193 Here we screened phage-displayed peptide libraries and identified the 13-

194 e screened two variable cysteine-constrained phage-displayed peptide libraries for factor H-binding p

195 Towards this goal, we biopanned three phage-displayed peptide libraries on a series of well-de

196 As a proof of concept, we produced phage-displayed peptide libraries Ser-[X]4-Gly-Gly-Gly,

197 o study the affinity and binding kinetics of phages, displaying peptide libraries.

198 ition of a small molecular ink with screened phage displayed peptides.

199 achieve successful in vitro selection of T7 phage-displayed peptides that recognize markers expresse

200 Competitive immunoscreening of phage-displayed peptides was applied to select mimotopes

201 ptor, from a library of approximately 10(11) phage-displayed peptides, which binds PSMA with high aff

202 nding parameters of 26 different filamentous phages, displaying peptides selective for enhanced Green

203 We adapted a competitive peptide phage display platform to identify candidate peptides bi

204 In this work, we established a phage-display platform to select for specific amidation,

205 The extended phage display procedure provides a generic way to non-na

206 oped a dedicated approach, proteomic peptide-phage display (ProP-PD), to identify domain-SLiM interac

207 y technology was used to screen a library of phage displaying random 12-mer peptides for those that b

208 e-containing tetrapeptides by constructing a phage-display random tetrapeptide library and conducting

209 In addition, epitope mapping with a phage-displayed random peptide library revealed that one

210 A phage-displayed recombinant antibody library was used to

211 ere fully replicated in the target-selected, phage-displayed repertoire.

212 We used random peptide phage display, reverse immunization, and next-generation

213 Phage-display scFv hybrid libraries of allergic donor-de

214 A T7 phage display screen against full-length human ERalpha,

215 al Syp1 cargo-sorting motifs, we performed a phage display screen and used biochemical methods to dem

216 rthermore, another peptide from the original phage display screen, midgut peptide 2 (MP2), strongly i

217 Using an in vivo phage-display screen, we identify a peptide, targeted ax

218 Phage display screening allows the study of functional p

219 eptide (sequence CAQK) identified by in vivo phage display screening in mice with acute brain injury.

220 Phage display screening is exploited to select 12mer pep

221 In vivo single-chain Fv (scFv) antibody phage display screening using a human synovial xenograft

222 15-amino acid peptide (15-mer), isolated via phage display screening, targeted Abeta and attenuated i

223 (HAP) of 15 residues was identified through phage-display screening followed by saturation mutagenes

224 t very high throughput using systems such as phage display, screening for functional properties (e.g.

225 We performed in vivo peptide phage display screens in mice bearing 4T1 metastatic bre

226 e sequences that are typically identified in phage display screens published to date.

227 Based on the reactive loop sequences of the phage display-selected inhibitors, we recombinantly expr

228 ed protein (GRP78), a receptor that binds to phage-display-selected ligands, such as the SNTRVAP moti

229 Phage display selection and downstream characterization

230 target-unrelated peptide (TUP) can arise in phage display selection experiments as a result of a pro

231 In vivo phage display selection is a powerful strategy for direc

232 SHC]OH (TCP-1), a small peptide derived from phage display selection, for targeting human CRC xenogra

233 hains, catechols, and sequences derived from phage display selection.

234 Phage display selections against peptides were used to g

235 selectively bind to CMG2, here we performed phage display selections using magnetic beads having bou

236 NT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.

237 Using a phage display single-chain antibody (scFv) library, we d

238 cognized by a monoclonal antibody (3C3G3) by phage display, site-directed mutagenesis, and surface pl

239 Particularly, a critical element in phage display sorting is functional immobilization of ta

240 ng peptide (CBP) as an immobilization tag in phage display sorting.

241 A phage-display strategy is presented whereby approximatel

242 The phage display system permitted the generation of protein

243 Using a novel phage display system, we discovered an adhiron that shar

244 terminal tail of bZIP28 were identified in a phage display system.

245 element (DGR) provides a naturally occurring phage-display system, but engineering efforts are hamper

246 the affinity selection capabilities of other phage display systems.

247 e then subjected to in vitro selection using phage display technique and 3 clones (CSP3, CSP4 and CSP

248 cted from a random peptide library using the phage display technique.

249 of allergen mimotopes identified through the phage display technique.

250 ed diagnostic and therapeutic agents, is the phage-display technique.

251 g a competitive inhibition strategy, we used phage display techniques to identify 53 single-chain var

252 IgG)4kappa- and IgG4lambda-Fab library using phage display technology and by Epstein-Barr virus trans

253 Phage display technology and combinatorial peptide chemi

254 In this paper, we combined the phage display technology and electrochemical impedance s

255 ed using sequences from immunized alpaca and phage display technology for antibody selection.

256 ors that reduce intestinal toxicity, we used phage display technology to generate highly specialized

257 vel treatment for these individuals, we used phage display technology to target the insulin receptor

258 In this study, phage display technology was used to develop a monoclona

259 In this study, phage display technology was used to screen a library of

260 Phage display technology was used to select affibody mol

261 Using phage display technology, Fynomers were generated inhibi

262 Using phage display technology, human antigen-binding fragment

263 onisin B1 using a novel mimotope selected by phage display technology.

264 We selected Fab fragments using the phage display technology.

265 Taking advantage of phage-display technology, we engineered a fully human si

266 this challenge using a peptide identified by phage display termed skin penetrating and cell entering

267 we generated high-affinity SUMO2 variants by phage display that bind the back side binding site of Ub

268 eukaryotic display technology comparable to phage display that would overcome the protein translatio

269 A naturally occurring analog to phage display, the Bordetella bronchiseptica bacteriopha

270 Here, we used phage display to develop specific ubiquitin-based inhibi

271 E and other pKal-mediated disorders, we used phage display to discover a fully human IgG1 monoclonal

272 next-generation sequencing-assisted antibody phage display to establish a highly myeloma-specific epi

273 We present the first report of the use of phage display to identify novel activities toward insect

274 parallel in vivo and in vitro selection with phage display to identify novel tumor-homing ACPPs with

275 Recent reports have expanded phage display to include membrane proteins (MPs).

276 We used phage display to isolate peptides that possess bona fide

277 Here, to further characterize HBeAg, we used phage display to produce a panel of chimeric rabbit/huma

278 cted-diversity combinatorial Fab library and phage display to rapidly generate synthetic antibodies (

279 Here, we use phage display to select a peptide that binds preferentia

280 We have used Phage Display to select scFv (single-chain variable frag

281 We used phage-display to produce a melanoma-specific TCR (alpha2

282 Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide m

283 Phage-displayed tumor antigens were enriched by biopanni

284 We employed a phage-displayed ubiquitin variant (UbV) library to devel

285 TP4 was obtained by phage display using the four-repeat Tau construct K18Del

286 from their heavy-chain only antibodies in a phage display vector and selected nanobodies (VHHs) agai

287 Selection via phage display was used first to enrich the library betwe

288 Previously, a Fab against Rev generated by phage display was used to crystallize and solve the stru

289 Phage display was used to further enhance the affinity o

290 Here, phage display was used to identify variants of human TIM

291 contact beta-lactamase were randomized, and phage display was used to sort the libraries for tight b

292 By using phage display we identified specific positions that clus

293 Through the use of a T7 phage display, we discovered a novel interaction between

294 With the aid of secretome phage display, we identified a highly conserved protein

295 Using structure-based design and phage display, we modified the initial inhibitors to gen

296 by native chemical ligation and mirror image phage display, we recently identified a D-peptide inhibi

297 By using combinatorial phage display, we screened selected VH genes paired with

298 Using in vivo phage display, we searched for molecular markers of the

299 chain encoded by VH3-30, was isolated using phage display with immobilized hemagglutinin (HA) from i

300 ative high-throughput approach that combines phage display with second generation sequencing.