ライフサイエンスコーパス: phage lambda

1 miniscent of the arrangement established for phage lambda.

2 y element" derived from the cI/Cro region of phage lambda.

3 omal region flanking the attachment site for phage lambda.

4 circuitry of HK620 seems to resemble that of phage lambda.

5 HK022 is a temperate coliphage related to phage lambda.

6 of phage HK022 are resistant to infection by phage lambda.

7 Escherichia coli ihf mutants for plating of phage lambda.

8 discriminate between different operators in phage lambda.

9 genes of Escherichia coli or to the rest of phage lambda.

10 n the interaction of IHF with the H' site of phage lambda.

11 ne activity in the DNA packaging reaction of phage lambda.

12 tion of the gpD protein during maturation of phage lambda.

13 lyze the stability of the lysogenic state of phage lambda.

14 2 intrinsic transcription terminator site of phage lambda.

15 ontrolling the direction of recombination in phage lambda.

16 tudied tyrosine recombinases such as that of phage lambda.

17 in E. coli and the lysis/lysogeny switch of phage lambda.

18 ve applied this approach to the circuitry of phage lambda.

19 chanistically distinct from the integrase of phage lambda.

20 reminiscent of the Rz/Rz1 lysis gene pair of phage lambda.

21 dified an existing bistable circuit, that of phage lambda.

22 ed to selection for immunity to infection by phage lambda.

23 human cDNA expression library constructed in phage lambda.

24 solated a full-length mouse P2X3 gene from a phage lambda-129/Sv genomic library.

25 The N protein of phage lambda acts with Escherichia coli Nus proteins at

26 complete understanding of gene regulation by phage lambda also requires detailed knowledge of the rol

27 e-stranded DNA binding proteins, such as the phage lambda and D108 repressors, which undergo substant

28 Using Phage Lambda and Escherichia coli as models we selective

29 wo sets of plasmids, pL and pE, that contain phage lambda and Escherichia coli K-12 chromosomal DNA f

30 e component is related to the exonuclease of phage lambda and is common to viruses with linear double

31 alpha-helical peptide from the N protein of phage lambda and its cognate 19 nucleotide box B RNA hai

32 ation at specific loci on the chromosomes of phage lambda and its Escherichia coli host.

33 long-circulating mutants of Escherichia coli phage lambda and of Salmonella typhimurium phage P22.

34 , and DNA sequencing analysis of recombinant phage lambda and P1 clones revealed that exons encoding

35 sed largely on work done on the integrase of phage lambda and recombinases like Cre, Flp, and XerC/D.

36 id family genome sequences, those of E. coli phage lambda and Salmonella typhimurium phage P22.

37 ut smears were found for digests of DNA from phage lambda and several plasmids.

38 d biophysical properties of DNA packaging in phage lambda and, in particular, the nucleoprotein compl

39 n latency and productive replication in both phage-lambda and HIV-1.

40 The DNA-packaging specificities of phages lambda and 21 depend on the specific DNA interact

41 The integrases of phages lambda and HK022 are closely related members of t

42 Analysis of phages lambda and HK022 by Weisberg and collaborators pr

43 Phages lambda and HK022 express proteins N and Nun, resp

44 nes are nearly identical to Escherichia coli phages lambda and HK97, S. enterica phage ST64T, or a Sh

45 Site-specific recombination by phages lambda and P22 is carried out by multiprotein-DNA

46 en evolutionarily diverse bacterial viruses (phages lambda and P22), as well as a eukaryotic virus, h

47 g to form lethal lesions, or "holes." In the phages lambda and T4, the holes have been shown to be la

48 sults to the interaction patterns of E. coli phages lambda and T7.

49 de a tyrosine recombinase similar to that of phage lambda, and xis (excisionase, sacV).

50 g scanning calorimetry on a bacterial virus (phage lambda) as an experimental model system, we invest

51 A phage lambda-based recombination system, Red, can be use

52 In the wild-type phage lambda, binding of CI to O(R)2 helps polymerase bo

53 Nun binds the phage lambda boxB RNA sequence (BOXB) on nascent lambda

54 s left operator region (compared to three in phage lambda), but this has a minimal effect on 933W lys

55 from prophage HK022 excludes superinfecting phage lambda by arresting transcription on the lambda ch

56 The phage HK022 Nun protein excludes phage lambda by binding nascent lambda pL and pR transcr

57 Phage HK022 Nun protein excludes phage lambda by binding nascent lambda-nut RNA and induc

58 ich linear DNA fragments were generated from phage lambda by intracellular EcoRI restriction followin

59 ts obtained on the canonical tR2 template of phage lambda by means of complementary bulk gel electrop

60 d for DNA translocation into the capsid, the phage lambda capsid decoration protein gpD is essential

61 acing of double-stranded DNA encapsidated in phage lambda capsids.

62 Much of the gene regulatory circuitry of phage lambda centers on a complex region called the O(R)

63 atalyzes the integration and excision of the phage lambda chromosome into and out of the Esherichia c

64 Packaging a phage lambda chromosome involves cutting the chromosome

65 cherichia coli, we monitored the behavior of phage lambda chromosomes, repressed or not for lambda ge

66 LexA and other cleavable substrates, such as phage lambda CI repressor and E. coli UmuD, bind to a cl

67 en oligomerization of PrgX was tested with a phage lambda cI repressor fusion system, the oligomeriza

68 rized DNA-looping proteins Lac repressor and phage lambda CI to measure interactions between pairs of

69 tion and mapping of a cluster of overlapping phage lambda clones from a BCBL tumor DNA genomic librar

70 The phage lambda-derived Red recombination system is a power

71 the organization in the tail gene cluster of phage lambda, despite a lack of amino acid sequence simi

72 I and ECO:RV endonucleases has been shown on phage lambda DNA and with BCL:I and DPN:II endonucleases

73 Feasibility was tested using Bcl I and phage lambda DNA as a model enzyme and amplicon system,

74 anY, an E. coli protein that is required for phage lambda DNA injection, was found to localize to the

75 gp2.5 lowers the phage lambda DNA melting force as measured by single mol

76 six plasmids that contain BamHI fragments of phage lambda DNA were constructed and transformed into E

77 from a gamma-globin promoter by fragments of phage lambda DNA.

78 Phage lambda encodes two recombination proteins that are

79 In the case of wild-type phage lambda excision junctions, spermidine plays the do

80 se fold, whose structural prototypes are the phage lambda exonuclease, the very short patch repair nu

81 ch is required for site-specific excision of phage lambda from the bacterial chromosome, has a much s

82 omes that integrate and excise the genome of phage lambda from the Escherichia coli chromosome.

83 The product of phage lambda gene N appears to function in a stoichiomet

84 ase (RNAP) to an antiterminating form by the phage lambda gene Q protein.

85 ption pause, and simultaneously to allow the phage lambda gene Q transcription antiterminator to act.

86 perimental tests of this question, using the phage lambda gene regulatory circuit.

87 Here we test whether the behavior of the phage lambda gene regulatory circuitry is robust.

88 monstrate in this paper that Beta protein of phage lambda generates recombinants in chromosomal DNA b

89 nases, stimulate integration and excision of phage lambda genome, regulate the transcription of sever

90 te two strong Fis binding sites in the 50 kb phage lambda genome.

91 194M downstream of the Walker B motif in the phage lambda gpA packaging motor causes an 8-fold reduct

92 g the wheat transcriptome was constructed in phage lambda gt11 and screened with IgE antibodies from

93 esponding cDNA from an expression library in phage lambda gt11.

94 3' sequence: TC TAAGTAGTTGATTCATA, where the phage lambda H1 consensus sequence of IHF is underlined)

95 structure of the Cro repressor protein from phage lambda has been refined to a crystallographic R-va

96 or site specific recombination of Tn1545 and phage lambda, has been developed.

97 ntrol N protein dependent antitermination in phage lambda have counterparts in many eukaryotic system

98 upplied with either single host (allopatry), phage lambda improved its binding to the available recep

99 Studies of phage lambda in vivo have indicated that its own recombi

100 l S. aureus strain SA564, and in restricting phage lambda infection when the endonuclease is expresse

101 ulations using parameter values derived from phage lambda-infection also showed an optimal lysis time

102 The final step of lysis in phage lambda infections of Escherichia coli is mediated

103 IN on T-mediated lysis in the context of the phage lambda infective cycle, in the absence of other T4

104 cessary to resolve Holliday junctions during phage lambda Int-mediated recombination.

105 Escherichia coli phage lambda integrase (Int) is a 40 kilodalton, 356 ami

106 fferent site-specific recombination enzymes, phage lambda integrase and transposon Tn3 resolvase.

107 The Escherichia coli phage lambda integrase protein (Int) belongs to the larg

108 viously identified hexapeptide inhibitors of phage lambda integrase-mediated recombination block the

109 ange events leading to Holliday junctions in phage lambda integration and excision are asymmetric, pr

110 which terminase, the DNA packaging enzyme of phage lambda, introduces staggered nicks into viral conc

111 The integrase protein (Int) of phage lambda is a well-studied representative of the tyr

112 The gene regulatory circuitry of phage lambda is among the best-understood circuits.

113 The Rap protein of phage lambda is an endonuclease that nicks branched DNA

114 The lysogenic state of phage lambda is maintained by the CI repressor.

115 EGS construct is used in vivo, burst size of phage lambda is reduced by > or = 40%.

116 final steps in the morphogenetic pathway of phage lambda is the packaging of a single genome into a

117 the switch from lysogenic to lytic growth of phage lambda is the self-cleavage of lambda repressor, w

118 Ejection of the genome from the virus, phage lambda, is the initial step in the infection of it

119 g functionally similar to its counterpart in phage lambda, it shows no homology at the level of prote

120 laof plasmid pBR322; (iii) the PLpromoter of phage lambda; (iv) and (v) the replication control promo

121 In previous work with phage lambda, lethal mutations that changed ATP-reactive

122 l N-protein-dependent antitermination in the phage lambda life cycle have counterparts in the regulat

123 The phiKO2 virion is unusual in that its phage lambda-like tails have an exceptionally long (3,43

124 Here we show that regulated expression of phage lambda lysis genes S and R causes dramatic lysis o

125 ic phenotype selection is analyzed using the phage lambda lysis-lysogeny decision circuit as a model

126 mportant noise sources in gene expression of phage lambda lysogen are quantified using models describ

127 Some of the dispensable features of phage lambda may be evolutionary refinements.

128 e demonstrate by inserting the chromosome of phage lambda (minus a region apparently unstable in Esch

129 A td intron-phage lambda model system was developed to analyze exon

130 of Rho-dependent transcription termination, phage lambda N and rRNA transcription antitermination, a

131 RNase P from Escherichia coli can cleave phage lambda N mRNA in vitro or in vivo when the mRNA is

132 Stabilization of these contacts by phage lambda N protein leads to antitermination.

133 n vivo and in vitro at pause sites distal to phage lambda N-Utilization (nut) site RNA sequences.

134 h motif of the phage HK022 Nun protein binds phage lambda nascent mRNA transcripts while the carboxy-

135 nd binds to the N utilization (nut) sites on phage lambda nascent RNA and induces transcription termi

136 nine-rich motif of coliphage HK022 Nun binds phage lambda nascent transcript, whereas the carboxyl-te

137 HK022 Nun protein binds to NUT sequences in phage lambda nascent transcripts and induces transcripti

138 In this sense, phage WO might be likened to phage lambda of the endosymbiont world.

139 pression of a gene(s) from the nin region of phage lambda partially complemented both the viability a

140 studies of the closed complex formed on the phage lambda prmup-1 Delta265 promoter under reaction co

141 n and prenicking at various positions of the phage lambda prmup-1Delta265 promoter DNA on the rate of

142 sible; however, here we demonstrate that the phage lambda procapsid can be expanded with urea in vitr

143 tment of the phosphorylated CTF doublet with phage lambda protein phosphatase eliminated the 20- to 2

144 The enzymes of phage lambda provide a model for understanding a recombi

145 eam of the gene encoding the analogue of the phage lambda Q transcription activator with its site of

146 like the Redbeta synaptase component of the phage lambda recombinase.

147 mediated recombination distinguishes it from phage lambda recombination, in which the phage recombina

148 y be more relevant to the process of in vivo phage lambda recombination.

149 a two-subunit recombinase reminiscent of the phage lambda Red alpha/beta recombination system and tha

150 n this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under

151 we find that both Escherichia coli RecET and phage lambda Red recombination proteins function ineffic

152 model for viral recombination, based on the phage lambda Red recombination system, is proposed.

153 ets recombinant joint molecules arising from phage lambda Red-mediated genetic exchange.

154 PCR mutagenesis, overlap extension PCR, and phage lambda Red-mediated homologous recombination and t

155 Phage lambda-Red proteins are powerful tools for pulling

156 ucture and energy of the encapsidated DNA in phage lambda regulates the mobility required for its eje

157 We have addressed this question using the phage lambda regulatory circuit, which can persist in tw

158 ein, CI, is essential to the behavior of the phage lambda regulatory circuit.

159 s seen in other groups of phages such as the phage lambda-related group of phages of enteric hosts an

160 new high resolution crystal structure of the phage lambda repressor reveals the basis for repressor d

161 -studied cooperative interactions is that of phage lambda repressor, which binds cooperatively to two

162 orts real-time measurements of ejection from phage lambda, revealing how the speed depends on key phy

163 t of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chr

164 Cosmids are plasmids that contain the phage lambda sequences (cos) required for packaging of t

165 d to trap Holliday junction intermediates of phage lambda site-specific recombination in vivo.

166 Salmonella chromosome architecture using the phage lambda site-specific recombination system as a pro

167 Peptide inhibitors of phage lambda site-specific recombination were previously

168 fitness, we constructed a series of isogenic phage lambda strains that differ only in their late prom

169 Isogenic phage lambda-strains with different combinations of six

170 hod to the dynamics of reduced models of the phage lambda switch, and show that the switching times b

171 ally, we present data which demonstrate that phage lambda terminase can efficiently utilize DNA from

172 Phage lambda terminase carries out the cos cleavage reac

173 ve thus constructed a deletion mutant of the phage lambda terminase gpNu1 subunit which constitutes a

174 y are displayed as fusions to the surface of phage lambda that are marked with different selectable d

175 Compared to phage lambda, the covalent crosslinking at the icosahedr

176 n N regulates the transcriptional program of phage lambda through recognition of RNA enhancer element

177 p in bacteriophage infection, the docking of phage lambda to its membrane receptor maltoporin, at the

178 e results suggest a remarkable adaptation of phage lambda to the environment of its host bacteria in

179 ntly, the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized rec

180 predicted to adopt the beta-sandwich fold of phage lambda TTP.

181 We have recently found that DNA packaged in phage lambda undergoes a disordering transition triggere

182 Recently, the holin S gene of phage lambda was overexpressed and the holin protein was

183 minal portion of the cl repressor protein of phage lambda was used as a reporter of dimerization in E

184 e microscopy nanoindentation measurements of phage lambda, we previously proposed a minimal model des

185 al class I lambda holin, the S105 protein of phage lambda, which controls lysis by forming holes in t

186 previously shown that the Cro repressor from phage lambda, which is a dimer, can be converted into a

187 Phage lambda with the chimeric terminase is unable to fo

188 ction of each copy, a helper plasmid bearing phage lambda xis and int genes is introduced into the ce

189 ent on DNA similar to that described for the phage lambda Xis protein.