ライフサイエンスコーパス: phagosome

1 sed by pharmacological neutralization of the phagosome.

2 AP by excluding the p22phox subunit from the phagosome.

3 tant for ubiquitin dynamics on the bacterial phagosome.

4 lated proteins in response to the macrophage phagosome.

5 omotes virulence by detecting the macrophage phagosome.

6 increase in cryptococcal killing within the phagosome.

7 cellular membranes, including the bacterial phagosome.

8 of LLO-neutralizing molecules in the forming phagosome.

9 of the autophagy machinery, like LC3, to the phagosome.

10 erial interaction site before closure of the phagosome.

11 toplasma yeasts remain within the macrophage phagosome.

12 s required for LC3 recruitment to the fungal phagosome.

13 effector function of Irgm1 at the bacterial phagosome.

14 d induced more lipid raft recruitment to the phagosome.

15 important roles during the maturation of the phagosome.

16 esulting from an inability to neutralize the phagosome.

17 sure chloride levels in phagocytes and their phagosomes.

18 nd localized to the site of partially formed phagosomes.

19 rless strain of Yersinia remains confined to phagosomes.

20 3,4,5)P3, and it persists on PI(3)P-enriched phagosomes.

21 hat ensure efficient killing of pathogens in phagosomes.

22 s of spores, macrophages fail to form mature phagosomes.

23 uired for the recruitment of mitochondria to phagosomes.

24 ly and specifically recruited to early-stage phagosomes.

25 and antigen presentation among TLR-signaling phagosomes.

26 d progressively degrade them within maturing phagosomes.

27 eins, and Elmo1 to phagocytic cups and early phagosomes.

28 and intracellular survival within acidified phagosomes.

29 -associated protein 1 light chain 3 (LC3) to phagosomes.

30 spectrum--to compare the properties of their phagosomes.

31 ng, is recruited directly by LC3 to Dectin-1 phagosomes.

32 gocytosis, which sequesters pathogens within phagosomes.

33 rly p40phox(+) phagosomes into late LAMP1(+) phagosomes.

34 ced proton-pumping activity compared with M2 phagosomes.

35 at Irgm1 targets mycobacterial and listerial phagosomes.

36 hosphatidic acid was nearly identical in all phagosomes.

37 pecifically within the acidifying macrophage phagosomes.

38 ta stp2Delta cells, and occupied more acidic phagosomes.

39 many minutes to engulf these filaments into phagosomes.

40 mation, RVs persisted around bead-containing phagosomes.

41 osis, entrapping them into organelles called phagosomes.

42 I molecules enhances peptide accumulation in phagosomes.

43 ribution and accumulation of cleaved TLR9 to phagosomes.

44 ing Rac2 that maintains a near neutral pH of phagosomes.

45 -, A. fumigatus-, and C. albicans-containing phagosomes.

46 receptor 9 (TLR9)-mediated sensing of DNA in phagosomes.

47 sed inhibition of T3S function, allowing for phagosome acidification and bacterial killing.

48 r et al. discover an intriguing link between phagosome acidification and lipid signposts on their out

49 Indeed, T3S prevented phagosome acidification and resisted killing inside thes

50 vh mutant with virD4 also reversed entry and phagosome acidification defects in broth cultures.

51 ernalization, identifying a critical role of phagosome acidification to facilitate microbial digestio

52 d for the elimination of invading organisms, phagosome acidification, and maturation.

53 utants are defective in entry and evasion of phagosome acidification.

54 the MRSA USA300 clone, the USA300-containing phagosome acidified rapidly and acquired the late endoso

55 cells and organelles (e.g., residual bodies, phagosomes) across the seminiferous epithelium.

56 represent a general mechanism for restoring phagosome activity in conditions, where it is lost or im

57 LC3 recruitment to the phagosome also requires Syk signaling but is independent

58 s, including the popular SIINFEKL, can enter phagosomes also via a second unknown energy-dependent me

59 In addition, inhibitors of phagosome and autophagosome maturation reversed the bene

60 ELMO1 is present in the phagosome and enhances bacterial clearance by differenti

61 on, both at an early stage in the macrophage phagosome and later within the necrotic granuloma.

62 al ingested inoculum survives within the PMN phagosome and likely contributes directly or indirectly

63 by releasing reactive oxygen species in the phagosome and release neutrophil extracellular traps (NE

64 f macrophages, the organism escapes from its phagosome and replicates to high density in the cytosol,

65 nslocation of the autophagy machinery to the phagosome and ultimately LC3 conjugation.

66 the coassembly of NADPH oxidase subunits at phagosome and/or plasma membranes.

67 h defects in the transport of spermatids and phagosomes and a disruption of cell adhesion most notabl

68 ycobacterium tuberculosis (Mtb) lives within phagosomes and also disrupts these organelles to access

69 ompartment, unable to be recycled to nascent phagosomes and autophagosomes.

70 utophagy pathway to facilitate maturation of phagosomes and digestion of their contents.

71 ins play critical roles in the maturation of phagosomes and endosomes following microbial recognition

72 rotease MarP for Mtb to survive in acidified phagosomes and establish and maintain infection in mice.

73 ive intracellular pathogen that escapes from phagosomes and grows in the cytosol of infected host cel

74 he spatiotemporal dynamics of Mtb-containing phagosomes and identified an interferon-gamma-stimulated

75 in the delivery of lysosomal hydrolases into phagosomes and in digestion of the cargo.

76 rsistent MPs, prevent fusion between damaged phagosomes and intact lysosomes and thereby preserve end

77 nd Slc30a7; the metal was shuttled away from phagosomes and into the Golgi apparatus.

78 ophils and macrophages, engulf microbes into phagosomes and launch chemical attacks to kill and degra

79 r one round of phagocytosis and suggest that phagosomes and lysosomes are capable of bi-directional s

80 nd occasionally oxidative environment within phagosomes and lysosomes.

81 somal trafficking, prevent the maturation of phagosomes and modify many signaling pathways inside of

82 ent macrophage mitochondria are recruited to phagosomes and produce mitochondrially derived reactive

83 nhibits the maturation of R. equi-containing phagosomes and promotes intracellular bacterial survival

84 , hence altering the centripetal movement of phagosomes and their maturation.

85 on of NADPH oxidase and the heterogeneity of phagosomes and, finally, consider the importance of two

86 ated with an inability to evade the maturing phagosome) and were significantly attenuated in murine a

87 or the cleavage of clathrin-coated vesicles, phagosomes, and mitochondria.

88 However, because pure and tightly sealed phagosomes are difficult to obtain, direct evidence for

89 These Atg8/LC3-positive phagosomes are formed around the antigen with TLR2 agoni

90 internal contents visible, while accumulated phagosomes are frequently docked to cathepsin D-positive

91 ce delivery of MHC-II-peptide complexes from phagosomes are not known.

92 at PtdIns(3)P behaves similarly in canonical phagosomes as well as endosomes.

93 odo-SAFs report pH in both the cytoplasm and phagosomes, as well the nucleus.

94 aB-kinase (IKK)2-mediated phosphorylation of phagosome-associated SNAP23.

95 uld correlate well with phagocytosis because phagosomes become acidified and the average fluorescence

96 The phagosome becomes a dynamic hub receiving traffic from m

97 t of Golgi-derived secretory vesicles during phagosome biogenesis, which was important for uptake of

98 nt generation occurs within an intracellular phagosome, but diffusible species can react with the neu

99 that GFP-Dectin-1 translocates to the fungal phagosome, but its signal decays after 2 h.

100 se with non-matured mycobacterium-containing phagosomes, but fusion events with mycobacterium-contain

101 uent in high zinc conditions, e.g. inside of phagosomes, but that it can be replaced by S18-2 when zi

102 ts confirm that TAP can import peptides into phagosomes, but they suggest that some peptides, includi

103 this study, we monitored peptide import into phagosomes by flow cytometry using two types of fluoresc

104 ciation with p85 results in encapsulation of phagosomes by lipidated LC3 in multi-membrane organelles

105 rbation that pathogens encounter within host phagosomes, carbon source deprivation, which leads to tr

106 These pools are rapidly mobilized to phagosomes carrying microbial antigens, and in a signal-

107 how that MHC-I selectively accumulate within phagosomes carrying microbial components, which engage T

108 howed normal particle engulfment but delayed phagosome clearance and reversed diurnal profiles of lev

109 tion, nuclear translocation of TFEB requires phagosome completion and fails to occur in cells silence

110 ant increased the proportion of DAG-positive phagosomes, concomitantly potentiating phagosomal NOX ac

111 ify GOP-1 as essential for the maturation of phagosomes containing apoptotic cells, through recruitme

112 al acidification blocks TLR9 accumulation on phagosomes containing beta-1,3 glucan beads.

113 Phagosomes containing Candida albicans also require Dect

114 LC3-associated phagocytosis (LAP), in which phagosomes containing engulfed particles, including dyin

115 Phagosomes containing live C. albicans cells became tran

116 However, fewer phagosomes containing live USA300 bacteria than those co

117 ike phagosomes containing wild-type bacilli, phagosomes containing the DeltacpsA mutant recruited NAD

118 Unlike phagosomes containing wild-type bacilli, phagosomes cont

119 The maturation of phagosomes, defined by the presence of late endocytic ma

120 The degradation of phagosomes, derived from the ingestion of photoreceptor

121 al tubules are not essential for delivery of phagosome-derived MHC-II-peptide complexes to the plasma

122 By contrast, M1 phagosomes displayed alkaline pH oscillations, which wer

123 the cell surface (and then ultimately to the phagosome during phagocytosis) in response to G-protein

124 als (autophagy), is recruited to traditional phagosomes during internalization of extracellular paylo

125 Phagosome-early endosome fusion required PI(3)P, yet did

126 o one another, exhibited profound defects in phagosome escape and intracellular growth, and appeared

127 ith SCHU S4 DeltapurMCD, a mutant capable of phagosome escape but of only limited cytosolic replicati

128 red for intracellular replication even after phagosome escape into the cell cytosol.

129 pathogenicity island, required for bacterial phagosome escape, intracellular replication, and virulen

130 ge death, as evidenced by the failure of the phagosome escape-deficient mutant SCHU S4 DeltafevR to i

131 beta-1,3-glucan phagosomes expressing a signaling incompetent Dectin-1 f

132 We show that in DCs, maturing phagosomes extend numerous long tubules several hours af

133 gested that NOX activation is precluded when phagosomes fail to reach a critical DAG concentration.

134 ts influence on the maturation of macrophage phagosomes following the engulfment of C. albicans cells

135 cquisition, intracellular chloride supply to phagosomes for oxidant production, and methods to measur

136 Mitochondria need to be juxtaposed to phagosomes for the synergistic production of ample react

137 Phagosomes form during engulfment of large particles and

138 HB2, to encompass bacteria both during early phagosome formation and after L. monocytogenes escaped t

139 atidylinositol 4-phosphate (PtdIns4P) during phagosome formation and maturation.

140 calcium sequestration, and calcium-dependent phagosome formation around secondarily encountered ACs i

141 gene expression from disease onset involving phagosome formation as well as natural killer cell and I

142 tein expression, cell-to-cell differences in phagosome formation, and number of bacterium engulfed.

143 intaining L. monocytogenes in a bactericidal phagosome from which it cannot escape.

144 osome, the events related to endolysosome-to-phagosome fusion do not significantly differ between the

145 stabilizes SNARE complexes orchestrating ERC-phagosome fusion, enrichment of phagosomes with ERC-deri

146 ive intracellular pathogen that escapes from phagosomes, grows in the host cytosol, and avoids autoph

147 LC3's association with phagosomes has been implicated in regulating microbial k

148 , direct evidence for peptide transport into phagosomes has remained limited.

149 , which generates superoxide within maturing phagosomes, has also been shown to regulate activities o

150 These bacteria can escape the phagosome in host cells by using the pore-forming toxin

151 lbicans infection but is dysregulated on the phagosome in the presence of the invasive hyphal form, w

152 xamined the fate of the S. aureus-containing phagosome in THP-1 macrophages by evaluating bacterial i

153 e or heat-killed Candida albicans-containing phagosomes in a Dectin-1-dependent manner in GM-CSF-deri

154 tide with high TAP affinity is imported into phagosomes in a TAP- and ATP-dependent manner, as expect

155 und that Keap1 associated with mycobacterial phagosomes in a time-dependent manner, whereas siRNA-med

156 Phagosomes in DCs are also signaling platforms for patte

157 V but not to T4SS mutant organism-containing phagosomes in human macrophage-like cells, primary human

158 hagy marker LC3 localized to single-membrane phagosomes independently of the ULK complex, which is re

159 TLR signaling adaptors TRAM and MyD88 to the phagosome, indicating that the TLR-MyD88 signaling compl

160 ecreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-

161 and phagocytosis, supported by evidence that phagosomes induced in Cln3(Deltaex1) (-) (6)-derived mou

162 macrophages, and is required to preserve Mtb phagosome integrity and control Mtb replication.

163 echanisms that contribute to maintaining Mtb phagosome integrity have not been investigated.

164 Escape of F. tularensis from the phagosome into the cytosol of the macrophage triggers th

165 t facilitates maturation of early p40phox(+) phagosomes into late LAMP1(+) phagosomes.

166 acteria they enclose them in small vesicles (phagosomes) into which superoxide is released by activat

167 lts indicate that the Histoplasma-containing phagosome is limiting for riboflavin and pantothenate an

168 at signals the transition from early to late phagosomes is accompanied by resurgence of PtdIns4P, whi

169 orting of luminal and membrane proteins into phagosomes is critical for the immune function of this o

170 osphoinositides (PIPs) have been detected on phagosomes, it remained unclear which PIPs actually gove

171 mitochondrial trafficking and mitochondrion-phagosome juxtaposition.

172 inase beta abrogated DAG accumulation at the phagosome, leading to impaired respiratory burst.

173 ptidoglycan in macrophage and dendritic cell phagosomes leads to activation of the NLRP3 inflammasome

174 However, fungal germination in the late phagosome led to macrophage necrosis.

175 ome speed, but run length was decreased, and phagosome localization and degradation were impaired.

176 s to characterize the third band as the cone phagosomes located in the top of the RPE.

177 ogen Mycobacterium tuberculosis by enhancing phagosome lysosome maturation, an event resulting from I

178 hagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic mach

179 Additionally, PIKfyve inactivation blocks phagosome-lysosome fusion in a manner that can be rescue

180 licates in macrophages in part by inhibiting phagosome-lysosome fusion, until interferon-gamma (IFNga

181 es that generate these PIPs, as mediators of phagosome-lysosome fusion.

182 nalized POS vesicles were colocalized with a phagosome marker, suggesting that ABCF1-mediated engulfm

183 esting that lysosomal Ca(2+) released during phagosome maturation activates TFEB.

184 in monocyte/macrophages and to have roles in phagosome maturation and cell activation.

185 ting lysosomal protein that are required for phagosome maturation and fusion with lysosomes.

186 IFN-gamma signaling via Mal was required for phagosome maturation and killing of intracellular Mycoba

187 the proneurotrophin receptor sortilin during phagosome maturation and mycobacterial killing.

188 By comparing the phagosome maturation and the subsequent MHC class II pre

189 . paratuberculosis pathogenesis, we examined phagosome maturation associated with transcriptional res

190 Thus, PI(3)P regulates phagosome maturation at early and late stages, whereas P

191 Therefore, Rab14 activity promotes phagosome maturation during C. albicans infection but is

192 bris buster (dsb), act at distinct stages of phagosome maturation for dendrite clearance.

193 how for the first time that M. bovis arrests phagosome maturation in a process that depends on P27.

194 sxG (TB9.8) and EsxH (TB10.4), which impairs phagosome maturation in macrophages and is essential for

195 In the Mreg(-/-) mouse where phagosome maturation is delayed, there was a temporal sh

196 epletion delayed the acquisition of the late phagosome maturation markers LAMP1 and lysosomal catheps

197 Phagosome maturation requires sequential fusion of the p

198 d propose a model in which PIKfyve modulates phagosome maturation through phosphatidylinositol-3,5-bi

199 h reporter peptides is shortened by enhanced phagosome maturation triggered by TLR signaling.

200 s in wild-type mice and mice with defects in phagosome maturation using a mouse RPE explant model.

201 Recent studies link TLR activation with phagosome maturation via the engagement of autophagy pro

202 nd lysosomal compartments, a process called 'phagosome maturation', which leads to the degradation of

203 inked to the biological functions autophagy, phagosome maturation, and lytic vacuole/lysosome, and co

204 erculosis lipoproteins and lipoglycans block phagosome maturation, inhibit class II MHC Ag presentati

205 te that corpses persist because of defective phagosome maturation, rather than recognition defects.

206 Tubular lysosomes have been implicated in phagosome maturation, retention of fluid phase, and anti

207 This subset includes genes involved in phagosome maturation, superoxide production, response to

208 uggests that TORC1 regulates Draper-mediated phagosome maturation.

209 d cellular activation affect many aspects of phagosome maturation.

210 remained unclear which PIPs actually govern phagosome maturation.

211 y viral protein (Vpr) was crucial to perturb phagosome maturation.

212 r efficient clearance of apoptotic cells and phagosome maturation.

213 tension generation, membrane recycling, and phagosome maturation.

214 feration of engulfed pathogens by expediting phagosome maturation.

215 brane recycling, and, in macrophages, blocks phagosome maturation.

216 oplasmic reticulum (calreticulin)-associated phagosome maturation.

217 yelinase (ASMase) and required for efficient phagosome maturation.

218 fusing with lysosomes, in a process termed "phagosome maturation." In this issue, Yin et al. identif

219 lating microbial killing, Ag processing, and phagosome maturation; however, the mechanism by which LC

220 Phagosomes mature into phagolysosomes by sequential fusi

221 rt of cytosolic peptides into dendritic cell phagosomes, mediated by TAP transporters recruited from

222 at ATP7A-dependent copper transport into the phagosome mediates host defense against S Typhimurium, w

223 with M.tb DK9897 being unable to disrupt the phagosome membrane and make contact to the cytosol.

224 rotein 1A/1B-light chain 3) is conjugated to phagosome membranes using a portion of the canonical aut

225 Rac signaling axis is critical for effective phagosome-mitochondrion function and bactericidal activi

226 Live-cell imaging showed that the phagosomes moved bidirectionally along microtubules in R

227 In the macrophage phagosome, NRAMP1 removes Mn and other essential metals

228 is (Mtb) survives the normally bacteriocidal phagosome of macrophages.

229 ability of this bacterium to escape from the phagosome of the host cells via the action of the pore-f

230 s that the redox microenvironment within the phagosomes of APCs is a determinant in MHC class II repe

231 The chemistries within phagosomes of APCs mediate microbial destruction as well

232 The phagosomes of M1 macrophages had a similar buffering pow

233 to survive and replicate in the endosomes or phagosomes of the host cells and establish persistent in

234 d as a direct effector molecule on bacterial phagosomes or on other organelles or as an inducer of au

235 Although some remodel phagosomes, others rapidly escape into the cytosol of in

236 cysteine cathepsin proteolytic efficiency in phagosomes, particularly in the absence of high NADPH ox

237 assical complement system and its associated phagosome pathway.

238 By contrast, M2 phagosomes proceed to acidify immediately in order to cl

239 cassette A4 transporter results in defective phagosome processing and accumulation of lipid debris.

240 the overall uptake of fungal cells, or early phagosome processing.

241 hat C. albicans-driven neutralization of the phagosome promotes hyphal morphogenesis, sufficient for

242 p91(phox) and p22(phox) were recruited to Gc phagosomes regardless of bacterial Opa expression.

243 w chloride is intracellularly transported to phagosomes remain largely unknown.

244 ermining peptide uptake by, and survival in, phagosomes remain little characterized.

245 LC3 II recruitment in A. fumigatus phagosomes required spleen tyrosine kinase (Syk) kinase-

246 LC3 recruitment to fungal phagosomes requires activation of the fungal pattern rec

247 king of TLR9 to A. fumigatus and C. albicans phagosomes requires Dectin-1 recognition.

248 ium tuberculosis, plays an essential role in phagosome rupture and bacterial cytosolic translocation

249 culosis (Mtb) and plays an essential role in phagosome rupture and translocation to the cytosol of ma

250 Soon after the phagosome seals, PtdIns4P levels drop precipitously due

251 n prolonged retention of GFP-Dectin-1 to the phagosome signifying a link between Syk and intraphagoso

252 Lack of KLC1 did not inhibit phagosome speed, but run length was decreased, and phago

253 When FYCO1 is lacking, phagosomes stay p40phox(+) longer and produce more react

254 Although TLR signaling from phagosomes stimulates presentation of phagocytosed antig

255 d lysosomal-associated membrane protein-1 to phagosomes, suggesting attenuated microbicidal activity.

256 ion by enabling maximal cargo transfer among phagosomes that bear a TLR signature.

257 d rafts that are retained in the membrane of phagosomes that do not undergo lysosomal maturation.

258 with live bacteria inside flotillin-enriched phagosomes that had access to nutrients provided by the

259 of phagocytic cups as well as to the nascent phagosomes that harbor Gal/GalNAc lectin and actin.

260 hosphatidylinositol 3-monophosphate-positive phagosomes that mature into phagolysosomes using a pathw

261 des many natural environments, including the phagosomes that mediate cell-based immunity.

262 After formation of a phagosome, the events related to endolysosome-to-phagoso

263 Phagosomes then fuse with lysosomes and granules to kill

264 Phagosomes then fuse with lysosomes to mature into phago

265 AMP1)-positive and LysoTracker-positive late phagosomes; these characteristics were similar in both n

266 isms were degraded readily within neutrophil phagosomes, thus indicating that survival in the neutrop

267 n up into MSC endosomes colocalized with Mtb phagosomes, thus suggesting that the latter were fusion

268 This phagosome to vacuole invasion (PTVI) pathway may represe

269 was, in turn, promoted by the failure of M1 phagosomes to acidify.

270 g RILP, which were shown earlier to displace phagosomes toward perinuclear lysosomes.

271 critical regulator of endoplasmic reticulum-phagosome traffic required for cross-presentation.

272 However, how phagosomes transmit signals to recruit mitochondria has

273 These results elucidate mechanisms of POS phagosome transport in relation to degradation, and demo

274 KD testes displayed defects in spermatid and phagosome transport, and also spermatid polarity due to

275 RVs fused with lysosomes, whereas associated phagosomes typically did not.

276 Phagosomes undergo fusion and fission events with endoso

277 cruitment of the autophagy protein LC3 II in phagosomes upon fungal cell wall swelling.

278 ates of individual mycobacteria delivered to phagosomes versus phagolysosomes and discovered that bac

279 eptor is acquired by mycobacteria-containing phagosomes via interactions with the adaptor proteins AP

280 I molecules rather than SIINFEKL import into phagosomes via TAP.

281 Despite localization to acidified late phagosomes, viable G. bethesdensis cells were recovered

282 The paucity of V-ATPases in M1 phagosomes was associated with, and likely caused by, de

283 The duration of Rab14 retention on phagosomes was prolonged for hyphal cargo and was direct

284 P. stomatis, 52% of the bacterium-containing phagosomes were enriched for the specific granule marker

285 Here, POS phagosomes were observed to associate with myosin-7a, an

286 take of the NPs; ii) nanoparticle-containing phagosomes were rapidly processed into phagolysosomes, w

287 N expression increases inside the neutrophil phagosome, where MPO is located, compared with outside t

288 proteases, support the microbial killing in phagosomes, where most neutrophil-mediated antimicrobial

289 acrophages, facilitating its escape from the phagosome, which can result in host cell lysis.

290 s internalization of the microorganisms into phagosomes, which are then delivered to endolysosomes fo

291 R9 trafficking to beta-1,3 glucan-containing phagosomes, which may be critical in coordinating innate

292 t microbial intruders by engulfing them into phagosomes, which subsequently mature into microbicidal

293 maturation requires sequential fusion of the phagosome with early endosomes, late endosomes, and lyso

294 strating ERC-phagosome fusion, enrichment of phagosomes with ERC-derived MHC-I, and subsequent cross-

295 might be caused by a defect in the fusion of phagosomes with lysosomes, while this process occurs rap

296 e described in the endosomes, lysosomes, and phagosomes with the potential to catalyze the reduction

297 nalyzed the involvement of PIPs in fusion of phagosomes with various endocytic compartments and ident

298 Phagosome-with-lysosome fusion (PLF) results in the deli

299 homolog) complex to both macropinosomes and phagosomes within a minute of internalization.

300 ion, although noncanonical autophagy targets phagosomes, xenophagy was required to restrict the growt