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3 primarily associated with nutrient storage (phaseolin) and carbohydrate metabolism (lectins) were si
4 nd that the levels of phosphorylation of the phaseolin changed remarkably in the seed transition from
6 ved for another assembly defective mutant of phaseolin, delta360, which remains monomeric and does no
8 oduction of transcripts encoding the protein phaseolin during seed embryogenesis but is silent in veg
10 in isolate fractions, and B1, B2, and B3 for phaseolin fractions) were assayed for antioxidant and me
13 e results suggest that ABA induction of beta-phaseolin gene expression is modulated by developmental
15 Thus, fractions coming from the isolate and phaseolin had similar activities except for iron chelati
17 orts the hypothesis that mobilization of the phaseolin in germinating seeds occurs through the degrad
18 e synthesis, accumulation and degradation of phaseolin in the common bean seed are not yet sufficient
19 difference in the electrophoretic pattern of phaseolin, indicating an interaction between phaseolin a
21 s except for iron chelation, suggesting that phaseolin is the major contributor to the antioxidant an
25 our results indicate that post-translational phaseolin modifications through phosphorylations need to
29 sence of transcriptional expression from the phaseolin (phas) gene promoter in leaf tissue and that t
36 us to identify differentially phosphorylated phaseolin polypeptides in dormant and germinating seeds
37 on the phaseolin digestibility in vitro and phaseolin-polyphenol complexation obtained by SDS-PAGE o
38 e show that in tobacco, expression of a beta-phaseolin promoter-GUS (PHSbeta-uidA) mRNA and the corre
39 ng for phytohemagglutinin L-subunit and beta-phaseolin, respectively, is coordinately induced in bean
41 and Trp-rich sequence to the C-terminus of a phaseolin variant lacking its vacuolar sorting signal, w
42 d tryptophan of the trimeric storage protein phaseolin, we fused a Met- and Trp-rich sequence to the
44 nits of type alpha and beta that compose the phaseolin were identified by matrix-assisted laser desor
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