ライフサイエンスコーパス: phosducin

1 ma interacts with its regulators, Galpha and phosducin.

2 d ethanol-responsive gene, is a homologue of phosducin, a known major regulator of Gbetagamma signali

3 sponse, while also investigating the role of phosducin, a phosphoprotein binding transducin betagamma

4 A key component in dark/light adaptation is phosducin, a phosphorylatable protein that modulates the

5 e also show that Tbetagamma association with phosducin, a photoreceptor-specific protein of unknown p

6 We suggest that the mechanism of phosducin action is based on the reduction of transducin

7 transducin beta gamma subunits interact with phosducin along their entire intracellular translocation

8 the abundant photoreceptor-specific protein phosducin and found that the ON-bipolar cell responses i

9 udy, we identify new isoforms of the retinal phosducin and investigate the expression of the phosduci

10 Phosducin and its isoforms are widely distributed in bod

11 otoreceptor transduction proteins, cytosolic phosducin and membrane-bound rhodopsin, by the same enzy

12 Phosducin and phosducin-like protein (PhLP) bind G prote

13 d the functional roles of the two domains of phosducin and phosducin-like protein in binding retinal

14 Phosducin and phosducin-like protein regulate G protein

15 ice to unravel the function of recoverin and phosducin and to further define the role of the gamma su

16 ansgenic mouse models expressing full-length phosducin, and phosducin lacking phosphorylation sites s

17 Mammalian phosducins are known to bind G protein betagamma subunit

18 kinase 2 or membrane-targeted myristoylated-phosducin-attenuated or abolished Cav2.3 modulation.

19 al change upon interaction of betagamma with phosducin (beta1H311Agamma2, beta1R314Agamma2, and beta1

20 Together, these data indicate that phosducin binding to transducin beta gamma subunits faci

21 the Gtalpha binding surface, explaining how phosducin blocks Gtbetagamma's interaction with Gtalpha.

22 ng surface plasmon resonance showed that: 1) phosducin bound G(t)betagamma with a 2.5-fold greater af

23 Phosphorylation of phosducin by Ca(2+)/calmodulin-dependent protein kinase

24 These data indicate that phosducin can selectively regulate early signaling event

25 onformational change when the dimer binds to phosducin (conformational change mutants).

26 gamma dimer and two regions of defined Gbeta/phosducin contact points.

27 hosducin-like protein; 2) phosphorylation of phosducin decreased its affinity by 3-fold, principally

28 ovine retinal protein phosphatase 2A (PP2A), phosducin dephosphorylation activity peaks coelute with

29 with the B alpha regulatory subunit have no phosducin dephosphorylation activity.

30 the cytosolic fraction of mouse retina, the phosducin dephosphorylation of which occurs rapidly.

31 tein with limited homology to members of the phosducin family that associates with baculovirus Op-IAP

32 sducin and investigate the expression of the phosducin family, showing that an isoform, PhLP1, has se

33 omparable results were observed when the GST-phosducin fusion proteins selectively sequestered Gbeta

34 chemical mechanism for the regulation of the phosducin-Gt beta gamma interaction.

35 However, although phosducin has a well characterized role in retinal photo

36 Phosducin has two domains that wrap around Gtbetagamma t

37 These results support the role of phosducin in regulating signaling in the rod outer segme

38 We also found that the amount of phosducin in rods is sufficient to interact with practic

39 retina neurons and transgenic expression of phosducin in rods of phosducin knock-out mice rescued th

40 lines of evidence in support of the role of phosducin in transducin translocation.

41 Here we describe two homologues of phosducin in yeast, called PLP1 and PLP2.

42 nificant clusters of identity with mammalian phosducin, including a domain corresponding to a highly

43 Although the molecular basis of phosducin interaction with G proteins is well understood

44 Phosducin is a major phosphoprotein of rod photoreceptor

45 Phosducin is a photoreceptor-specific protein known to i

46 lls and that the subcellular distribution of phosducin is consistent with that of a soluble protein e

47 likely to be photoreceptor specific because phosducin is not expressed in other retina neurons and t

48 s may be generalized to other Gbetagamma and phosducin isoforms as well.

49 To test the functional interaction of all phosducin isoforms with Gbeta gamma in vitro, a glutathi

50 ransgenic expression of phosducin in rods of phosducin knock-out mice rescued the rod-specific phenot

51 from the sensitivity of phototransduction in phosducin knock-out rods being affected to a much lesser

52 Second, we generated a phosducin knockout mouse and found that the degree of li

53 models expressing full-length phosducin, and phosducin lacking phosphorylation sites serine 54 and 71

54 We have identified phosducin-like 3 (PDCL3, also known as PhLP2A), through

55 The phosducin-like orphan proteins (PhLOPs) fail to bind Gbe

56 ma binding capability, whereas two isoforms (phosducin-like orphan proteins, PhLOPs) share sequence h

57 A proposed function of PDC and phosducin-like protein (PDCL) is the sequestration of "f

58 Phosducin and phosducin-like protein (PhLP) bind G protein betagamma s

59 Phosducin-like protein (PhLP) is a broadly expressed mem

60 Phosducin-like protein (PhLP) is a widely expressed bind

61 Phosducin-like protein (PhLP) is a widely expressed bind

62 Phosducin (Pdc) and phosducin-like protein (PhLP) regulate G protein-mediate

63 Phosducin-like protein (PhLP), a widely expressed ethano

64 is facilitated by the ubiquitously expressed phosducin-like protein (PhLP), which is thought to act a

65 cytosolic chaperonin CCT and a cochaperone, phosducin-like protein 1 (PhLP1) for dimer formation.

66 f Gbetagamma, recent studies have shown that phosducin-like protein 1 (PhLP1) works as a co-chaperone

67 (CCT; also called TRiC) and its cochaperone phosducin-like protein 1 (PhLP1).

68 We identified phosducin-like protein 2, Plp2p (=PLP2), as an ATP-eluta

69 alpha, one G beta, one G gamma subunits and phosducin-like protein BDM-1 that have important roles i

70 al roles of the two domains of phosducin and phosducin-like protein in binding retinal G(t)betagamma.

71 association of the Gbeta-GPSM3 complex with phosducin-like protein PhLP and T-complex protein 1 subu

72 Phosducin and phosducin-like protein regulate G protein signaling path

73 A proteomics search for binding partners of phosducin-like protein, a co-chaperone for the cytosolic

74 agamma with a 2.5-fold greater affinity than phosducin-like protein; 2) phosphorylation of phosducin

75 Phosducin-like proteins are conserved regulatory compone

76 Given that the regulatory role of phosducin-like proteins may be influenced by protein kin

77 ter closing the channels by dark adaptation, phosducin or inactive Galphao (both sequester Gbetagamma

78 cells is confined to photoreceptors and that phosducin participates in the underlying molecular mecha

79 (PhLP) is a broadly expressed member of the phosducin (Pd) family of G protein betagamma subunit (Gb

80 Phosducin (Pd) is a Gbetagamma-binding protein that is h

81 Phosducin (Pd) is a widely expressed phosphoprotein that

82 The phosphoprotein phosducin (Pd) regulates many guanine nucleotide binding

83 Phosducin (Pd), a small protein found abundantly in phot

84 Phosducin (Pdc) and phosducin-like protein (PhLP) regula

85 Phosducin (PDC) has been shown in structural and biochem

86 Phosducin (Pdc) is a conserved phosphoprotein that, when

87 Phosducin (Pdc) is a G protein beta gamma dimer (G beta

88 Phosducin (Pdc), a highly conserved phosphoprotein invol

89 Phosducin (Pdc), a highly conserved phosphoprotein, play

90 From the subtraction library, cDNA for phosducin (PDC), a member of the phototransduction pathw

91 n or rod- and cone-specific proteins such as phosducin, peripherin/rds, and ROM1.

92 Phosducin (Phd) and Phd-like proteins (PhLPs) selectivel

93 We found that in the dark-adapted rods, phosducin phosphorylated at serine 54 is compartmentaliz

94 In contrast, phosducin phosphorylated at serine 71 is present in all

95 The degree of phosducin phosphorylation in the dark appeared to be les

96 In the phosducin phosphorylation mutants, the transducin alpha

97 this process is accelerated significantly by phosducin phosphorylation.

98 ion of phosducin; thus, it was proposed that phosducin plays a role in the light adaptation of G prot

99 icance of light-dependent phosphorylation of phosducin remains largely hypothetical.

100 s Ser-73, which when phosphorylated inhibits phosducin's function, points away from Gtbetagamma, towa

101 This transition disrupts phosducin's interface with Gt beta gamma, leading to the

102 Phosducin's Ser-73, which when phosphorylated inhibits p

103 pal light-regulated phosphorylation sites of phosducin, serine 54 and 71.

104 biquitin-proteasome pathway and suggest that phosducin serves to protect Tbetagamma following the lig

105 on by labeling with antibodies to rod opsin, phosducin, synaptophysin, calbindin D, and glial fibrill

106 -dependent protein kinase II, which inhibits phosducin-Tbetagamma complex formation, completely resto

107 netics, and contained approximately 50% more phosducin than wild-type controls.

108 e regulatory function proposed for mammalian phosducin, the genetic data presented in this report sug

109 Light promotes dephosphorylation of phosducin; thus, it was proposed that phosducin plays a

110 3) The binding of phosducin to G(t)betagamma diminishes the membrane parti

111 In mice expressing normal phosducin, transducin alpha and betagamma subunits retur

112 redistribution to the plasma membrane, less phosducin upregulation, and fewer calbindin D-labeled ho

113 In pursuit of the function of phosducin, we tested the hypothesis that it regulates th

114 Gbetagamma increases the current, whereas m-phosducin, which binds Gbetagamma without affecting the

115 The exception was phosducin, which localized to both rods and cones and, i

116 Moreover, phosducin, which produces dissociation of G(t)betagamma

117 ansducin's betagamma subunits complexed with phosducin, which regulates Gtbetagamma activity, has bee

118 ilon subunit, greatly prefers phosphorylated phosducin, with an activity several hundred times those