1  activity, as indicated by the production of phosphatidylethanol.

2 PLD, as shown by a sustained accumulation of phosphatidylethanol.

3 d both PMA-induced PLD-mediated formation of phosphatidylethanol (approximately 5-fold) and the hydro

4 -conjugated sphingols (C2 ceramides) blocked phosphatidylethanol formation indicating that phospholip

5  model membranes of phosphatidylcholines and phosphatidylethanols have been studied as a function of

6 alpha-thrombin-induced production of nuclear phosphatidylethanol in quiescent cells incubated in the

7 ned by formation of the PLD-specific product phosphatidylethanol in the presence of 0.5% ethanol.

8 te-labeled phosphatidylcholine conversion to phosphatidylethanol in the presence of 2% ethanol.

9                                              Phosphatidylethanol is formed when ethanol substitutes i

10 vo PLD activity, as assessed by detection of phosphatidylethanol levels using mass spectrometry (MS)

11 st, phosphatidylalcohols exhibited only 25% (phosphatidylethanol) or 5% (phosphatidylbutanol) of the

12 eased generation of the PLD-specific product phosphatidylethanol (PEt) (9.9-fold increases in [3H]-PE

13  (TPA) in the presence of ethanol synthesize phosphatidylethanol (PEt) instead of phosphatidic acid (

14 se in PLD activity, as indicated by elevated phosphatidylethanol (PEt) production.

15 ion reaction, resulting in the production of phosphatidylethanol rather than PA.