ライフサイエンスコーパス: phosphoglycolate

1 ycin-induced DSBs possess 3'-ends blocked by phosphoglycolate.

2 to ionize the C-2 proton of either 2-PGA or phosphoglycolate.

3 bound to the reaction-intermediate analog, 2-phosphoglycolate (2-PGA).

4 several previous studies, Ape1 hydrolyzed 3'-phosphoglycolates 25-fold more slowly than C-4-keto-C-1-

5 structure of the transition-state analogue 2-phosphoglycolate (2PG) bound to methylglyoxal synthase (

6 2-Phosphoglycolate (2PG) is one of the shared metabolites

7 tion of base-propenal and gapped DNA with 3'-phosphoglycolate (3'-PG) and 5'-phosphate (5'-P) ends.

8 DNA strand breaks containing 3'-phosphoglycolate (3'-PG) ends are the major lesions indu

9 sion [DNA single-strand breaks containing 3'-phosphoglycolate (3'-PG)] was examined using a novel, ch

10 mum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3' to the cleavage site, as well as 2

11 to 2',3'-cyclic phosphate, 3'-phosphate, 3'-phosphoglycolate, 5'- hydroxyl and 5'- phosphate, which

12 2) and leads to the same set of products (3'-phosphoglycolate, 5'-phosphate, and base propenal) as fo

13 methylene-2-furanone (6), oligonucleotide 3'-phosphoglycolates (7), malondialdehyde equivalents (8 or

14 les that of the substrate but differs from 2-phosphoglycolate, a tight binding inhibitor, and phospha

15 an energy-intensive process that recycles 2-phosphoglycolate, a toxic product of the Rubisco oxygena

16 of crystal structures with either Fru-6-P or phosphoglycolate, an analog of PEP, bound have shown tha

17 ze other 3'-end DNA alterations including 3'-phosphoglycolate and 3'-abasic sites, and exhibits 3'-nu

18 at mimic the binding geometry of PEP, namely phosphoglycolate and 3-phosphonopropionate, are found to

19 sites and formation of strand breaks with 3'-phosphoglycolate and 5'-phosphate ends.

20 2-phosphosulfolactate, (S)-2-phospholactate, phosphoglycolate and both enantiomers of 2-phosphomalate

21 vels of the photorespiratory intermediates 2-phosphoglycolate and glycine are increased under high CO

22 variety of other 3' adducts from DNA such as phosphoglycolates and abasic or apurinic/apyrimidinic (A

23 y and repair activities on 3'-phosphates, 3'-phosphoglycolates, and 3'-trans-4-hydroxy-2-pentenal-5-p

24 Upon the binding of phosphoglycolate, Arg-162 is virtually replaced by Glu-1

25 th earlier data showing that Tdp1 can use 3'-phosphoglycolate as a substrate, these data suggest that

26 of EDTA, catalyzed removal of glycolate from phosphoglycolate at a single-stranded 3' terminus to lea

27 a 38mer duplex, but acted more slowly on 3'-phosphoglycolates at a 19 base-recessed 3'-terminus, at

28 In combination with the results of 2-phosphoglycolate binding studies, a catalytic mechanism

29 observed that the structure with inhibitor (phosphoglycolate) bound, compared to the structure of wi

30 In the Mn(2+)-phosphoglycolate complex of lobster muscle enolase, the

31 Vectors with blunt or cohesive 3'-phosphoglycolate ends yielded single repair products cor

32 substrate with 5'-overhangs and recessed 3'-phosphoglycolate ends.

33 C-1-aldehydes in unbroken DNA strands and 3'-phosphoglycolate esters terminating strand breaks.

34 nown factors may contribute to removal of 3'-phosphoglycolate esters.

35 ls contain potent activity for removal of 3'-phosphoglycolates from single-stranded oligomers and fro

36 Recombinant Ape efficiently removed phosphoglycolates from the 3'-terminus of an internal 1

37 the photorespiratory pathway intermediates 2-phosphoglycolate, glycolate, and glycine, suggest that t

38 A 3'-phosphoglycolate had little effect on Artemis-mediated t

39 is enzyme catalyzed the dephosphorylation of phosphoglycolate in vitro with similar kinetic propertie

40 mplex differs from the Pb(2+)-PEP and Mn(2+)-phosphoglycolate interactions in two enzymatically inact

41 G).d(CCCAGTACTTTGG), where X represents a 3'-phosphoglycolate lesion next to a 5'-phosphate.

42 substrates, bearing either 3'-hydroxyl or 3'-phosphoglycolate moieties, was examined.

43 d containing a double strand break with a 3'-phosphoglycolate on a 3-base 3' overhang was incubated i

44 sequence ions and confirmed the presence of phosphoglycolate on the 3'-terminal fragments only.

45 was no detectable activity of Ape toward 3'-phosphoglycolates on 1 or 2 base protruding single-stran

46 However, 3'-phosphoglycolates on overhangs of 4-5 bases promoted Art

47 For a blunt end with either a 3'-phosphoglycolate or 3'-hydroxyl terminus, endonucleolyti

48 One 2-phosphoglycolate (P-glycolate) molecule is produced for

49 hen applied to the indirect effect, detected phosphoglycolate (pg) and thymine glycol (Tg).

50 Glycolate and 2-phosphoglycolate (PG) are 2-carbon monocarboxylic acids

51 s devised for quantitative measurement of 3'-phosphoglycolate (PG) termini on DSBs induced by the non

52 plex with Mn(2+) and the substrate analog, 2-phosphoglycolate (PGL), was determined by molecular repl

53 to the 3' end of DNA, and can also remove 3'-phosphoglycolates (PGs) formed by free radical-mediated

54 ate docking showed that the high-specificity phosphoglycolate phosphatase (PDB entry ) uses a single

55 totrophic bacteria, cbbZ was shown to encode phosphoglycolate phosphatase (PGP), whereas the identiti

56 ic analysis, we have characterized TA0175 as phosphoglycolate phosphatase from Thermoplasma acidophil

57 explored HAD family member (gene annotation, phosphoglycolate phosphatase), which we termed AUM, for

58 ucture with those of other type IIB enzymes (phosphoglycolate phosphatase, trehalose-6-phosphate phos

59 We identified that mammalian phosphoglycolate phosphatase, with an uncertain function

60 with Cl- ions are similar between TA0175 and phosphoglycolate phosphatase.

61 idence presented for TA0175 is indicative of phosphoglycolate phosphatase.

62 milar kinetic properties seen for eukaryotic phosphoglycolate phosphatase.

63 In contrast to previously studied phosphoglycolate phosphatases, ComB has a low pH optimum

64 erhang was incubated in human cell extracts, phosphoglycolate processing proceeded rapidly for the fi

65 Phosphoglycolate removal by Ape was detected as a shift

66 ngle repair products corresponding to simple phosphoglycolate removal followed by ligation, while a v

67 only blocked rejoining, but also suppressed phosphoglycolate removal, implying an early, essential,

68 cise 3'-blocking termini, e.g. phosphate and phosphoglycolate residues, from DNA.

69 ve inhibition by the intermediate analogue 2-phosphoglycolate, resulting from the loss of stabilizing

70 spectroscopic titration with the inhibitor 2-phosphoglycolate revealed that the mutants have a differ

71 btain homogeneous preparations of defined 3'-phosphoglycolate substrates for repair studies, 5'-(32)P

72 ate from single-stranded DNA containing a 3'-phosphoglycolate, suggesting a role for Tdp1 in repair o

73 -mediated double-strand break, with cohesive phosphoglycolate-terminated 3'-overhangs and a one-base

74 ficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3' overhang, while le

75 ein kinase (DNA-PK), Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends.

76 n, and neocarzinostatin, which all induce 3'-phosphoglycolate-terminated double strand breaks.

77 eps using a sensitive and highly specific 3'-phosphoglycolate-terminated oligonucleotide-based assay

78 Shorter 3'-phosphoglycolate-terminated overhangs and blunt ends wer

79 Single- and double-strand breaks bearing 3'-phosphoglycolate termini are among the most frequent les

80 e, labeled with [alpha-32P]dCTP, contains 3'-phosphoglycolate termini produced by bleomycin-catalyzed

81 leavage reactions reveal 5'-phosphate and 3'-phosphoglycolate termini that are derived from H-atom ab

82 ates scattered AP sites, and the DSB have 3'-phosphoglycolate termini that require Ape1 processing.

83 s site-specific double-strand breaks with 3'-phosphoglycolate termini were constructed and treated wi

84 i were joined much faster than those with 3'-phosphoglycolate termini, although both were equally eff

85 rand breaks which contain 3'-phosphate or 3'-phosphoglycolate termini.

86 proficiently cleaves DNA modified with a 3'-phosphoglycolate terminus.

87 nd partial duplexes, each having a single 3'-phosphoglycolate terminus.

88 ain of Glu-167 upon binding of the inhibitor phosphoglycolate trianion (I(3-)), an analog of the ened