ライフサイエンスコーパス: phosphohydrolase

1 gylcerol acyltranferase or phosphatidic acid phosphohydrolase).

2 encoding CNPase (2',3' cyclic nucleotide 3'-phosphohydrolase).

3 te for human diphosphoinositol polyphosphate phosphohydrolase.

4 rter suggests a unique role for this unusual phosphohydrolase.

5 res prior generation of DAG by phosphatidate phosphohydrolase.

6 ontains both annexin III and cyclic inositol phosphohydrolase.

7 an ADP-ribosyltransferase and a nucleotidase/phosphohydrolase.

8 he members of HAD and DDDD super families of phosphohydrolases.

9 - and/or Mg(2+)-activated ecto-5'-nucleotide phosphohydrolases.

10 ies: GDP/GTP exchange factors and nucleotide phosphohydrolases.

11 enzyme superfamily is the largest family of phosphohydrolases.

12 r may contribute to the catalytic effects of phosphohydrolases.

13 s not a substrate for type 2 lipid phosphate phosphohydrolases.

14 rce as compared with other P-loop nucleotide phosphohydrolases.

15 rs from previously described lipid phosphate phosphohydrolases.

16 rminal "HD domain," found in metal-dependent phosphohydrolases.

17 ikingly dependent on an active phosphatidate phosphohydrolase 1 (PAP-1).

18 ty of the Mg(+2)-dependent phosphatidic acid phosphohydrolase 1 (PAP-1).

19 homologue of Lcb3p, sphingosine-1-phosphate phosphohydrolase 1 (SPP-1), an endoplasmic reticulum res

20 onstrated that sphingosine-1-phosphate (S1P) phosphohydrolase 1 (SPP-1), which is located mainly in t

21 COX-2 expression and implicate phosphatidate phosphohydrolase 1 as a key regulatory component of eico

22 The enzyme ectonucleotide tri(di)phosphohydrolase-1 (ENTPD1, also known as CD39) on the s

23 Depletion of S1P phosphohydrolase-1 (SPP1), which degrades intracellular

24 bstrate, but BEL also inhibits phosphatidate phosphohydrolase-1 and a group VIB phospholipase A(2).

25 nt of protein kinase C and phosphatidic acid phosphohydrolase-1 hydrolase signaling.

26 The intracellular localization of S1P phosphohydrolase-1 necessitates the import of extracellu

27 specific actions of BEL on phosphatidic acid phosphohydrolase-1, p47(phox) phosphorylation, and apopt

28 e kinase 1 and its functional antagonist S1P phosphohydrolase-1.

29 gral membrane protein, human lipid phosphate phosphohydrolase-3 (hLPP-3) enzyme, which hydrolyzes pho

30 Mutation of the AdnB phosphohydrolase abolished duplex unwinding, consistent

31 Crippling the AdnA phosphohydrolase active site did not affect the rate of

32 of GMP to RNA kinetically differentiates the phosphohydrolase active site from the guanylyltransferas

33 Mutations of the phosphohydrolase active site of the AdnB subunit ablate

34 by members of two classes (types 1 and 2) of phosphohydrolase activities (PAPs).

35 effective inhibitor of type 1 phosphatidate phosphohydrolase activities and is only modestly effecti

36 presence of two cation-dependent nucleotide phosphohydrolase activities in membranes of Caenorhabdit

37 hat the GTP cyclohydrolase and pyrophosphate phosphohydrolase activities occur at independent sites,

38 te general acid catalyst was dispensable for phosphohydrolase activity and phosphoenzyme formation by

39 Furthermore, phosphatidic acid phosphohydrolase activity assays showed that the bromoen

40 279A-D280A and F272A-L273A proteins retained phosphohydrolase activity but sedimented as monomers.

41 um and centrifugation, while cyclic inositol phosphohydrolase activity in both of these tissues remai

42 tribution of annexin III and cyclic inositol phosphohydrolase activity in rat kidney and spleen indic

43 positions had no significant effect on Cet1 phosphohydrolase activity in vitro and had no effect on

44 e triphosphate tunnel that are essential for phosphohydrolase activity in vitro and in vivo: Arg393,

45 h enzymes exhibited divalent metal dependent phosphohydrolase activity in vitro toward phosphoserine-

46 The R321A and D425A proteins had full phosphohydrolase activity in vitro, but were profoundly

47 Thirteen residues required for the phosphohydrolase activity of CaCet1p (Glu287, Glu289, As

48 a substrate of this enzyme, suggesting that phosphohydrolase activity of SAMHD1 is regulated by dGTP

49 possesses a general nucleoside triphosphate phosphohydrolase activity that lacks a preference for th

50 ofar as their replacement by alanine reduced phosphohydrolase activity to <5% of the wild-type value.

51 Activation of SAMHD1 phosphohydrolase activity was tested under physiological

52 ne of the richest sources of cyclic inositol phosphohydrolase activity, possesses very little (immuno

53 serine abrogates phosphoenzyme formation and phosphohydrolase activity.

54 xin III, but has very little cyclic inositol phosphohydrolase activity.

55 III can be dissociated from cyclic inositol phosphohydrolase activity.

56 PGA mutase that also exhibited low levels of phosphohydrolase activity.

57 ur studies clearly show that cyclic inositol phosphohydrolase and annexin III are two different prote

58 While gp2 contains the phosphohydrolase and endonuclease activities of terminas

59 We conclude that the phosphohydrolase and helicase activities of NPH-II are e

60 m of action, propranolol, an inhibitor of PA phosphohydrolase and phosphatidylcholine biosynthesis, w

61 luenzae belongs to the "DDDD" superfamily of phosphohydrolases and is the prototype of class C nonspe

62 The majority of HAD members are phosphohydrolases and may be divided into three subclass

63 is protein requires functional HD (predicted phosphohydrolase) and DEXD/H (predicted helicase) domain

64 as well as acid phosphatase, naphthol-AS-BI-phosphohydrolase, and beta-glucosidase activities in som

65 A 5'-triphosphatase, nucleoside triphosphate phosphohydrolase, and guanylyltransferase activities of

66 , glycine N-methyltransferase, pyrophosphate phosphohydrolase, and protein phosphatase 1D were down-r

67 rotein coupled receptor, two lipid phosphate phosphohydrolases, and isoprenylation.

68 ty characteristic of the apyrase category of phosphohydrolases, and its sequence contains four motifs

69 ers of the HD-domain protein superfamily are phosphohydrolases, and newly discovered members are gene

70 only to diphosphorylated inositol phosphate phosphohydrolase as a candidate for regulating signaling

71 roposed molecular mechanism for an HD-domain phosphohydrolase based directly on substrate-bound cryst

72 suppressed the cold sensitivity of the Cet1 phosphohydrolase, but did not suppress the cs growth def

73 sphate (S1P) or inhibiting phosphatidic acid phosphohydrolase by propranolol (Ppl).

74 iana, two Mg(2+)-dependent phosphatidic acid phosphohydrolases called PAH1 and PAH2 act redundantly t

75 ansports Glu-6-P into the lumen of the ER, a phosphohydrolase catalytic subunit residing in the lumen

76 1), Glu(181), and Glu(183) are essential for phosphohydrolase chemistry and likely comprise the metal

77 lex unwinding, including those implicated in phosphohydrolase chemistry via transition state stabiliz

78 c protein (GFAP), 2;,3;-cyclic nucleotide 3;-phosphohydrolase (CNPase), and vimentin, suggesting that

79 ized protein that contains a lipid phosphate phosphohydrolase consensus motif.

80 obic enzyme, which contains the type 2 lipid phosphohydrolase conserved sequence motif, shows substra

81 HD1) is a recently described deoxynucleotide phosphohydrolase controlling the size of the intracellul

82 olichos biflorus contain a lectin/nucleotide phosphohydrolase (Db-LNP) that binds to the Nod factor s

83 isoforms of diphosphoinositol synthases and phosphohydrolases determines the rates of phosphorylatio

84 rostacyclin production via the phosphatidate phosphohydrolase/diacylglycerol lipase pathway.

85 Diphosphoinositol polyphosphate phosphohydrolase (DIPP) hydrolyzes diadenosine 5',5"'-P(

86 rat hepatic diphosphoinositol polyphosphate phosphohydrolase (DIPP) that cleaves a beta-phosphate fr

87 R as a novel diphosphoinositol polyphosphate phosphohydrolase (DIPP).

88 o new 19-kDa diphosphoinositol polyphosphate phosphohydrolases (DIPPs), named types 3alpha and 3beta,

89 sly characterized relaxases, including an HD phosphohydrolase domain and an N-terminal hydrophobic re

90 tion of the catalytic residues of the SAMHD1 phosphohydrolase domain or by a Thr-592 phosphomimetic m

91 A thermostable phosphosulfolactate phosphohydrolase (EC 3.1.3.-) catalyzing the second step

92 hosphatidate phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) activity by phospholipids

93 tidate phosphatase (PAP1, 3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) catalyzes the dephosphoryl

94 atidate (PA) phosphatase (3-sn-phosphatidate phosphohydrolase, EC 3.1.3.4) catalyzes the dephosphoryl

95 phosphatase (PTP; protein-tyrosine-phosphate phosphohydrolase, EC 3.1.3.48), called PTPepsilon.

96 atidate (PA) phosphatase (3-sn-phosphatidate phosphohydrolase; EC 3.1.3.4) activity.

97 Lumenal ecto-nucleoside tri- and di-phosphohydrolases (ENTPDases) of the secretory pathway o

98 endoplasmic reticulum as a phosphatidic acid phosphohydrolase enzyme (PAP-1) to catalyze the penultim

99 hibits dual functions as a phosphatidic acid phosphohydrolase enzyme in the triglyceride synthesis pa

100 HD domain phosphohydrolase enzymes are characterized by a conserve

101 However, isotope effects associated with phosphohydrolase enzymes involved in P remineralization

102 The diphosphoinositol phosphate phosphohydrolase enzymes of the Nudix protein family hav

103 t and DNA viruses comprise a novel family of phosphohydrolase enzymes with a common active site.

104 sphatase EcCet1 belongs to a metal-dependent phosphohydrolase family that includes the triphosphatase

105 which suggest that different members of the phosphohydrolase family vary in their reliance on certai

106 B-40 family, Diphosphoinositol polyphosphate phosphohydrolase family, Transcription Elongation Factor

107 DGPP phosphatases (DGPP phosphohydrolase) from Saccharomyces cerevisiae and Esch

108 HD-domain phosphohydrolases have nucleotidase and phosphodiesteras

109 totype human diphosphoinositol-polyphosphate phosphohydrolase (hDIPP1), we now describe new 21-kDa hu

110 type 2 human diphosphoinositolpolyphosphate phosphohydrolases (hDIPP2alpha and 2beta) are distinguis

111 Nucleoside triphosphate phosphohydrolase I (NPH I) is a single-stranded DNA-depe

112 Nucleoside triphosphate phosphohydrolase I (NPH I) is an essential component of

113 Vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH I) serves as the ATPase activity

114 COOH-terminal end of nucleoside triphosphate phosphohydrolase I (NPH I), an ATPase that is employed i

115 termination factor, nucleoside triphosphate phosphohydrolase I (NPH I), and ATP.

116 Vaccinia virus nucleoside triphosphate phosphohydrolase I (NPH-I) is a DNA-dependent ATPase tha

117 capping enzyme, and nucleoside triphosphate phosphohydrolase I, and two core proteins required for m

118 y factor X as NPH-I (nucleoside triphosphate phosphohydrolase-I), a virus-encoded DNA-dependent ATPas

119 C2 in human PMN is the first lipid phosphate phosphohydrolase identified for PSDP.

120 a virus RNA helicase nucleoside triphosphate phosphohydrolase II (NPH-II) result in the production of

121 cinia viral helicase nucleoside triphosphate phosphohydrolase-II (NPH-II) exhibits robust RNA helicas

122 that the major ecto-nucleoside triphosphate phosphohydrolase in the chicken liver membranes is an ec

123 NPH-II mutations that inactivated the phosphohydrolase in vitro were lethal in vivo, as judged

124 y studies using propranolol, a phosphatidate phosphohydrolase inhibitor.

125 prevention of PA dephosphorylation by the PA phosphohydrolase inhibitors propranolol and bromoenol la

126 and wunen2 encode Drosophila lipid phosphate phosphohydrolases, integral membrane enzymes that dephos

127 Cyclic inositol phosphohydrolase is a phosphodiesterase that cleaves the

128 n placenta and reported that cyclic inositol phosphohydrolase is identical to annexin III.

129 DGPP phosphatase (DGPP phosphohydrolase) is a membrane-associated 34-kDa enzyme

130 s known about the role of the 2 S1P-specific phosphohydrolase isoforms, SGPP1 and SGPP2, which cataly

131 ) and 2-keto-3-deoxy-9-O-phosphonononic acid phosphohydrolase (KDN9P phosphatase, KDN9PP).

132 eto-3-deoxy-d-manno-octulosonate 8-phosphate phosphohydrolase (KDO8P phosphatase, KDO8PP) and 2-keto-

133 ast, the long-chain sphingoid base phosphate phosphohydrolase Lcb3p is required for efficient ceramid

134 -sensitive, endoplasmic reticulum-associated phosphohydrolase, like G6Pase-alpha.

135 approaches have identified LECTIN NUCLEOTIDE PHOSPHOHYDROLASE (LNP) as a Nod factor-binding protein.

136 This lectin-nucleotide phosphohydrolase (LNP) has a substrate specificity chara

137 Unlike other known type 2 lipid phosphate phosphohydrolases (LPPs), but similar to the yeast ortho

138 SAMHD1 is a phosphohydrolase maintaining cellular dNTP homeostasis b

139 Collectively, our results suggest that SPP phosphohydrolase may regulate the dynamic balance betwee

140 protein as an ecto-5'-nucleoside diphosphate phosphohydrolase (NudP) involved in the degradation of e

141 annexin III co-migrates with cyclic inositol phosphohydrolase on ion exchange chromatography, while g

142 is clearly dissociated from cyclic inositol phosphohydrolase on ion exchange chromatography.

143 ization of the Bacillus megaterium HD domain phosphohydrolase OxsA, involved in the biosynthesis of t

144 oMet) radical enzyme, OxsB, and an HD-domain phosphohydrolase, OxsA, reveals that OXT-A is derived fr

145 wed that the disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana

146 BEL also inhibits cellular phosphatidic acid phosphohydrolase (PAP) activity in intact P388D1 macroph

147 phospholipase D (PLD) and phosphatidic acid phosphohydrolase (PAP) are two pivotal enzymes in this s

148 Phosphatidic acid phosphohydrolase (PAP) catalyzes the dephosphorylation o

149 The phosphatidic acid phosphohydrolase (PAP) inhibitor dl-propranolol also inh

150 Lipin-1 is a phosphatidate phosphohydrolase (PAP) required for the generation of di

151 PA is converted to DG by PA phosphohydrolase (PAP).

152 Mg2+-independent form of PA phosphatase (PA phosphohydrolase, PAP2) purified from rat liver catalyze

153 Experiments with phosphatidate phosphohydrolase (PAPH) and phospholipase D (PLD) inhibi

154 also inhibited islet cytosolic phosphatidate phosphohydrolase (PAPH), but the PAPH inhibitor proprano

155 and oligodendrocytes (anti-cyclic nucleotide phosphohydrolase polyclonal antibody).

156 like enzymes comprise a large superfamily of phosphohydrolases present in all organisms.

157 Moreover, inhibitors of phosphatidate phosphohydrolase (propranolol) and diacylglycerol lipase

158 zed as a diphosphorylated inositol phosphate phosphohydrolase represents the first example, in any an

159 biquitin is a substrate for the nucleotidase/phosphohydrolase, resulting in either transfer of ubiqui

160 f CD81 with the deoxynucleoside triphosphate phosphohydrolase SAMHD1.

161 rresponding diacylglycerols by phosphatidate phosphohydrolase, since diacylglycerols were much slower

162 the NTPase domains to form two composite NTP phosphohydrolase sites.

163 racterized mammalian sphingosine-1-phosphate phosphohydrolase (SPP1), an enzyme that specifically dep

164 ogy between SPP1s and the other type 2 lipid phosphohydrolases, SPP1s are significantly different and

165 ancient L-2-halo-acid dehalogenase and DDDD phosphohydrolase superfamilies, but is specific for doub

166 MTH1 is a member of the nudix phosphohydrolase superfamily of enzymes, and it is invol

167 a large family of hydrolytic enzymes, the HD-phosphohydrolase superfamily.

168 ased on sequence homology with LBP1, a lipid phosphohydrolase that regulates the levels of phosphoryl

169 recently cloned based on homology to a lipid phosphohydrolase that regulates the levels of phosphoryl

170 diesterases (PDEs) comprise a superfamily of phosphohydrolases that degrade 3',5'-cyclic nucleotides.

171 Apyrases are non-energy-coupled nucleotide phosphohydrolases that hydrolyze nucleoside triphosphate

172 a member of a new family of metal-dependent phosphohydrolases that includes the RNA triphosphatases

173 allest member of a family of metal-dependent phosphohydrolases that includes the RNA triphosphatases

174 ongs to the tunnel family of metal-dependent phosphohydrolases that includes the RNA triphosphatases

175 allest member of a family of metal-dependent phosphohydrolases that includes the RNA triphosphatases

176 xed with natural substrates) of an HD-domain phosphohydrolase, the Escherichia coli 5'-nucleotidase Y

177 fold rate enhancements that are generated by phosphohydrolases, the most powerful biological catalyst

178 f the fungal/viral family of metal-dependent phosphohydrolases, which are structurally and mechanisti

179 atase belongs to a family of metal-dependent phosphohydrolases, which includes the RNA triphosphatase

180 dition to catalyzing hydrolysis, a number of phosphohydrolases will catalyze a phosphate (oxygen)-wat

181 a family of viral and fungal metal-dependent phosphohydrolases with a distinctive capacity to hydroly

182 ng to a single family of metal-dependent NTP phosphohydrolases with a unique tunnel active site compo