1 in myocytes lacking the regulatory protein,
phospholemman.
2 tion alters the interaction of Na/K-pump and
phospholemman.
3 We observed down-regulation of
phospholemman (
a phosphoprotein associated with anion ch
4 We previously identified FXYD1 (encoding
phospholemman;
a protein containing the motif phenylalan
5 We conclude that
phospholemman and Na/K-pump are in very close proximity
6 r inward-rectifier Cl- channels, e.g. ClC-2,
phospholemman and the channel in Xenopus oocytes.
7 y of small membrane proteins, represented by
phospholemman and the gamma subunit of Na,K-ATPase, was
8 t (CFP/YFP) fusion proteins of Na/K pump and
phospholemman and whether phospholemman phosphorylation
9 Phospholemman antibodies precipitated all three Na,K-ATP
10 pitation and functional studies suggest that
phospholemman associates with the Na/K-pump (NKA) and me
11 PKA targets, such as phospholamban at Ser16,
phospholemman at Ser68 and cardiac myosin-binding protei
12 of the Na(+),K(+)-ATPase modulating protein
phospholemman at serine residue 63 (S63 PLM).
13 Much like phospholamban regulation of SERCA,
phospholemman exists as both a sodium pump inhibiting mo
14 not NKA-alpha1 was coupled to a decrease in
phospholemman expression and phosphorylation, which woul
15 Furthermore,
phospholemman (
FXYD1) inhibits pump function without sig
16 Phospholemman (
FXYD1) is a homolog of the Na,K-ATPase ga
17 Phospholemman (
FXYD1) is a single-transmembrane protein
18 isoform and consistent with the presence of
phospholemman in both neurons and glia.
19 Here we show that
phospholemman is highly expressed in selected structures
20 t nearly abolished when co-expressing excess
phospholemman lacking YFP.
21 e-spanning membrane proteins such as cardiac
phospholemman,
Mat-8 and renal CHIF, large ion conductan
22 sphorylation signature associates with other
phospholemman molecules but not with the pump.
23 ospholemman phosphorylation, consistent with
phospholemman multimerization.
24 Phospholemman oligomers exist in cardiac muscle.
25 olubilized crude membranes using either anti-
phospholemman or anti-Na,K-ATPase antibodies.
26 without any differences in membrane markers (
phospholemman,
phosphalamban, dihydropyridine-binding co
27 s of Na/K pump and phospholemman and whether
phospholemman phosphorylation alters such FRET.
28 very close proximity (FRET occurs) and that
phospholemman phosphorylation alters the interaction of
29 This agreed with
phospholemman phosphorylation measurements: by protein k
30 photobleach), and this FRET was enhanced by
phospholemman phosphorylation, consistent with phosphole
31 nt experimental approaches demonstrated that
phospholemman physically associated with the Na,K-ATPase
32 In heart,
phospholemman (
PLM) inhibits NKA activity by reducing it
33 Phospholemman (
PLM) is a member of the FXYD ('fix-it') f
34 Phospholemman (
PLM) is a small (72-amino acid) transmemb
35 Phospholemman (
PLM) is a small transmembrane cardiac pro
36 e NKA-associated small transmembrane protein
phospholemman (
PLM) mediates beta-adrenergic-induced NKA
37 Phospholemman (
PLM) or FXYD1 is a major cardiac myocyte
38 Phospholemman (
PLM) phosphorylation mediates enhanced Na
39 The cardiac phosphoprotein
phospholemman (
PLM) regulates the cardiac sodium pump, a
40 Because
phospholemman (
PLM) regulates the Na(+)/K(+) pump (NKA)
41 The sarcolemmal protein
phospholemman (
PLM) was found associated with the Na/K A
42 afficking of two FXYD family members, FXYD1 (
phospholemman (
PLM)) and FXYD7.
43 We have demonstrated previously that
phospholemman (
PLM), a 15-kDa integral sarcolemmal phosp
44 Here, we show that
phospholemman (
PLM), a 15-kDa integral sarcolemmal phosp
45 Previous reports have demonstrated that the
phospholemman (
PLM), a 72-residue plasma-membrane protei
46 Phospholemman (
PLM), a major membrane substrate for phos
47 We have found that
phospholemman (
PLM), a member of the FXYD family and reg
48 Phospholemman (
PLM), a member of the FXYD family of prot
49 Phospholemman (
PLM), a member of the FXYD family of smal
50 arts) expression of Na/K-ATPase isoforms and
phospholemman (
PLM), a putative Na/K-ATPase regulatory s
51 eased, the palmitoylated regulatory protein,
phospholemman (
PLM), and the cardiac Na/Ca exchanger (NC
52 Phospholemman (
PLM), the principal quantitative sarcolem
53 Phospholemman (
PLM), the principal sarcolemmal substrate
54 eraction with a small transmembrane protein,
phospholemman (
PLM).
55 Phospholamban (PLB) and
phospholemman (
PLM, also called FXYD1) are small transme
56 Serine 68-phosphorylated
phospholemman (
pSer-68-PLM) inhibits NCX1 activity.
57 Antibodies against the C-terminal domain of
phospholemman reduced Na,K-ATPase activity in vitro with
58 Phospholemman regulates the plasmalemmal sodium pump in
59 Phospholemman was particularly enriched in choroid plexu
60 In cardiac muscle, a subpopulation of
phospholemman with a unique phosphorylation signature as