ライフサイエンスコーパス: phosphopeptide

1 Recovery is 90% for a pure phosphopeptide.

2 (D) of 0.35 nm in 1:1 binding to its cognate phosphopeptide.

3 antibody shows full specificity for a single phosphopeptide.

4 and is not significantly activated by an RTK phosphopeptide.

5 ammalian capping enzyme (Mce) bound to a CTD phosphopeptide.

6 o remove the NPE group and liberate a second phosphopeptide.

7 ysate, representing a more natural source of phosphopeptides.

8 omatography for the sequential enrichment of phosphopeptides.

9 cles to create membranes that rapidly enrich phosphopeptides.

10 uced efficiency, indicating a preference for phosphopeptides.

11 monoliths with surface layers selective for phosphopeptides.

12 ation and enrichment of different classes of phosphopeptides.

13 tion-specific amino acid patterns in sets of phosphopeptides.

14 parison with nano-LC-MS for the detection of phosphopeptides.

15 the quantitation of peptides as well as for phosphopeptides.

16 ation of different S-containing peptides and phosphopeptides.

17 analysis has been commonly used for mapping phosphopeptides.

18 ase of HNF-4alpha phosphorylation in several phosphopeptides.

19 sphate are frequently observed during CID of phosphopeptides.

20 the phosphorylation of 29 of 530 quantified phosphopeptides.

21 min), we carried out quantification of 2884 phosphopeptides.

22 hment provided more than 90% selectivity for phosphopeptides.

23 er been used for the selective enrichment of phosphopeptides.

24 dues, interfering with the identification of phosphopeptides.

25 R, and EDX and employed in the enrichment of phosphopeptides.

26 r abilities to enrich for different types of phosphopeptides.

27 ave selective affinity and can interact with phosphopeptides.

28 percentage of longer, basic, and hydrophilic phosphopeptides.

29 mined 14-3-3-binding motifs from non-binding phosphopeptides.

30 ble quantitation across tens of thousands of phosphopeptides.

31 ocols yielded comparable numbers of distinct phosphopeptides, 1693 and 1842, respectively, from micro

32 We provide temporal profiles of 4797 phosphopeptides, 608 of which showed significant regulat

33 he phosphoproteomic results of increased T31 phosphopeptide abundance with decreased MKK2 abundance i

34 Phosphopeptide abundances of five phosphoproteins involv

35 as also demonstrated on the basic and acidic phosphopeptides: acidophilic phosphorylation sites were

36 echniques that reproducibly measure the same phosphopeptides across multiple replicates, conditions,

37 ntibody fragment (Fab30) that recognizes the phosphopeptide-activated state of beta-arrestin-1.

38 ptide, and the interaction with the tyrosine phosphopeptide affects the pyruvate kinase activity of P

39 inase:substrate relationship analysis of the phosphopeptides also revealed ABL1 and SRC tyrosine kina

40 For phosphopeptide analysis by MALDI-MS, a 2,5-dihydroxybenz

41 Mass spectrometry-based phosphopeptide analysis demonstrated that the relative r

42 (i) ratio distortion remained a problem for phosphopeptide analysis in multiplexed quantitative work

43 /RP-nanoUPLC to compare their performance in phosphopeptide analysis.

44 ical binding interface to recognize the Cdc7 phosphopeptide and a non-canonical interface to bind Dbf

45 To this end, we performed and compared phosphopeptide and phosphoprotein enrichment methodologi

46 tative phosphoproteomic dataset yielded 2250 phosphopeptides and 1314 localized phosphosites with exc

47 enriched considerably longer and more acidic phosphopeptides and consequently, we identified 327 phos

48 ull range of values obtained using synthetic phosphopeptides and found excellent agreement.

49 localizing sites of phosphorylation in both phosphopeptides and intact phosphoproteins.

50 Phosphopeptides and lysine-acetylated peptides were quan

51 acid was used to overcome the degradation of phosphopeptides and provide high intensity phosphopeptid

52 r studying weak bonds between metal ions and phosphopeptides and provided a direct means of thermodyn

53 ID fragmentation analysis between unmodified phosphopeptides and those modified online with FBDSA or

54 monstrated that PKM2 interacts with tyrosine phosphopeptide, and the interaction with the tyrosine ph

55 A total of 8202 unique phosphopeptides, and 4317 unique proteins were identifie

56 ytes tested include steroids, phospholipids, phosphopeptides, and sialylated glycans.

57 esence of nano-TiO2 for selective binding of phosphopeptides, and the magnetic responsiveness of magn

58 signal intensities were enhanced for most pY-phosphopeptides (approximately 70%) when using the pY-MI

59 Oxygen atoms at phosphate groups of phosphopeptide are not exchanged.

60 thodologies to enrich heterogeneous types of phosphopeptides are critical for comprehensive mapping o

61 present study, the fragmentation patterns of phosphopeptides are improved through ion/ion-mediated pe

62 Phosphopeptides are of interest in enzymes and phosphory

63 To demonstrate the potential of these phosphopeptides as asymmetric catalysts, enantioselectiv

64 phosphoproteomic study identified additional phosphopeptides as possible targets that show the involv

65 Several studies identified short phosphopeptides as tight BRCT binders.

66 a designed phospholipid-inspired amphiphilic phosphopeptide at 0.8 A resolution is presented.

67 T231/pS235_1 Fab in complex with its cognate phosphopeptide at 1.9 A resolution.

68 serum samples are used for the detection of phosphopeptides based biomarkers.

69 re-affinity relationship studies showed that phosphopeptides based on Tyr631 from IL-4Ralpha bind wit

70 A phosphopeptide-based proteomic screen identified ROCK (R

71 7 phosphorylation sites from 13029 different phosphopeptides belonging to 3163 different phosphoprote

72 tivity to Src kinase while products (ADP and phosphopeptide) bind with positive cooperativity.

73 on of SHP2 without severely impairing proper phosphopeptide binding of N-SH2.

74 in evolution techniques and isolate new anti-phosphopeptide binding specificities from a library of v

75 an attractive avenue for generating new anti-phosphopeptide binding specificities in vitro by phage d

76 Phosphopeptide binding to p110beta/p85alpha and p110delt

77 bryonic DNA damage responses by means of its phosphopeptide-binding ability: activating Mnk in the nu

78 rupting effect on the structure of the N-SH2 phosphopeptide-binding cleft mediating the interaction o

79 pecificities from a library of variants of a phosphopeptide-binding domain.

80 HA1 domain and demonstrated that engineering phosphopeptide-binding domains is an attractive avenue f

81 Plant 14-3-3 proteins are phosphopeptide-binding proteins, belonging to a large fa

82 A triple mutation in the phosphopeptide-binding site of the forkhead-associated (

83 ures revealed that the monobodies occupy the phosphopeptide-binding sites of the SH2 domains and thus

84 profiling approaches, we found that a pY251 phosphopeptide binds specifically to a subset of SH2 dom

85 structures of inactive, preactivated p44 and phosphopeptide-bound arrestins and will guide our unders

86 ue generated a highly specific separation of phosphopeptides by an applied pH gradient between 9.2 an

87 tive approach for improved identification of phosphopeptides by sequential abundant ion fragmentation

88 re minimal differences between enrichment of phosphopeptides by TiO2 and Ti(4+)-IMAC when considering

89 Mapping of rat striatal DAT phosphopeptides by two-dimensional thin layer chromatogr

90 h throughput approach, based on high density phosphopeptide chips, to determine the in vitro substrat

91 In vitro phosphorylation assays and phosphopeptide competition experiments revealed a phosph

92 Mass spectroscopy analysis of Gp78 phosphopeptides confirmed S538 as a major p38 MAPK phosp

93 We identified a total of 2,001 phosphopeptides containing 1,026 unambiguous phosphoryla

94 derived for only a few 14-3-3 complexes with phosphopeptide-containing proteins and a variety of comp

95 Phosphoproteomic analysis revealed that 19 phosphopeptides corresponding to 12 proteins were differ

96 ptides, we extracted 849 uniquely identified phosphopeptides corresponding to 425 proteins and identi

97 And finally, phosphopeptides corresponding to MAP1B (corresponding to

98 Mass spectrometry revealed Tau phosphopeptides corresponding to Thr231, Ser235, Thr181,

99 n total, the SPS-MS3 method quantified 38247 phosphopeptides, corresponding to 11000 phosphorylation

100 In all, 6579 unique phosphopeptides, corresponding to 1701 unique phosphoryl

101 analysis identified that 35 serine/threonine phosphopeptides, corresponding to 27 proteins, were uniq

102 nt glycopeptides can be found in a published phosphopeptide data set.

103 ochemical properties of pY-MIP-TiO2-enriched phosphopeptides demonstrated that this protocol retrieve

104 rative evaluations of enrichment methods for phosphopeptides depend highly on the experimental protoc

105 was synergistic with activation by a soluble phosphopeptide derived from receptor tyrosine kinases.

106 ex with the consensus peptide as well as the phosphopeptide derived from SKAP-HOM.

107 Phosphopeptides derived from aberrantly phosphorylated p

108 these in vitro phosphorylated peptides with phosphopeptides derived from endogenous proteins isolate

109 The sizes of the tryptic phosphopeptides derived from Nup62 were compatible with

110 this method to detect and quantify predicted phosphopeptides derived from T-bet.

111 is study, we evaluated two HLA-A2-restricted phosphopeptides derived from the insulin receptor substr

112 It was also found that phosphopeptide detection limits were improved to approxi

113 -metal ion complexes during LC which hampers phosphopeptide detection.

114 A set of phosphopeptides differentially regulated by beta-glucan

115 ichment and Fourier transform MS analysis of phosphopeptides digested from a complex mixture containi

116 To date, hundreds of phosphopeptides displayed on melanoma, ovarian cancer, l

117 proteins, >300 protein kinases, and >15,000 phosphopeptides, enabled deep insight into signaling rec

118 14-3-3 isotypes gamma, theta, and zeta with phosphopeptides encompassing pS910, pS935, or pS1444 dem

119 thetic phosphopeptide reference data set and phosphopeptide-enriched samples.

120 d human blood serum, are used to explore its phosphopeptide enrichment ability from complex samples f

121 Furthermore, phosphopeptide enrichment analysis identified that 35 se

122 hieve precise quantitation, followed by TiO2 phosphopeptide enrichment and high resolution mass spect

123 The combination of TiO(2) phosphopeptide enrichment and MudPIT analysis revealed 8

124 ere determined by a strategic combination of phosphopeptide enrichment and nano-ultra-performance liq

125 In each phosphopeptide enrichment and purification process, only

126 For rapid and specific phosphopeptide enrichment and purification, sol-gel mate

127 Automated phosphopeptide enrichment demonstrates reproducible synt

128 As a result, automated phosphopeptide enrichment enables statistical analysis o

129 re demonstrated to be superior to TiO(2) for phosphopeptide enrichment from a complex mixture with hi

130 Phosphopeptide enrichment from complicated peptide mixtu

131 Phosphopeptide enrichment from digested peptide mixtures

132 Reproducible, comprehensive phosphopeptide enrichment is essential for studying phos

133 nd inactive states of kinases but performing phosphopeptide enrichment made it possible to measure th

134 ffinity purification, mass spectrometry, and phosphopeptide enrichment of isotope-labeled peptides, w

135 Phosphopeptide enrichment onto the synthesized amine-fun

136 We provide a free downloadable automated phosphopeptide enrichment program to facilitate uniform

137 been identified using these highly selective phosphopeptide enrichment protocols in combination with

138 The combination of pY-MIP- and TiO2-based phosphopeptide enrichment provided more than 90% selecti

139 tein phosphorylation that relies on a single phosphopeptide enrichment step using titanium dioxide mi

140 ere, we simplified the assay by removing the phosphopeptide enrichment step, increasing throughput wh

141 Here, we report a novel phosphopeptide enrichment strategy and its application t

142 se unique properties render the pY-MIP-based phosphopeptide enrichment technique an attractive altern

143 Most of them combine different phosphopeptide enrichment techniques and require startin

144 Moreover, phosphopeptide enrichment was successfully performed usi

145 e composite exhibits enhanced capability for phosphopeptide enrichment with sensitivity assessed to b

146 amino acids in cell culture, affinity-based phosphopeptide enrichment, and high-resolution mass spec

147 an inverse strategy based on TiO2 selective phosphopeptide enrichment, fractionation by strong catio

148 ication technology (MudPIT), without or with phosphopeptide enrichment, was applied to study the prot

149 d Ti-IMAC microspheres for uniform automated phosphopeptide enrichment.

150 re for visualization, and a biotin group for phosphopeptide enrichment.

151 the engineering of an affinity material for phosphopeptide enrichment.

152 The sol-gel network was then used for phosphopeptide enrichment.

153 chromatography can greatly assist selective phosphopeptide enrichment.

154 ifferent formats is checked in the selective phosphopeptides enrichment.

155 Robust phosphopeptide enrichments that are suitable for low inp

156 VVTITK, corresponding to Ser736, and an NF-H phosphopeptide, EPDDAKAKEPS*KP, corresponding to Ser942.

157 devised a strategy that enhances analysis of phosphopeptides, especially multiply phosphorylated pept

158 Phosphopeptide fishing indicated that SOCS6 binds direct

159 Measured phosphopeptide fold-changes from alpha-casein spiked int

160 reacted with HPO4(2-), phosphoserine, and a phosphopeptide (FQpSEEQQQTEDELQDK, abbreviated "betacas"

161 tituted up to 8% of the pY-MIP-TiO2-enriched phosphopeptide fractions.

162 fragments and comigration of PKC-stimulated phosphopeptide fragments with NDAT Ser-7 phosphopeptide

163 t in base signal-to-noise for distinguishing phosphopeptide from unphosphorylated peptide.

164 broblast cell lysate a total of 1,944 unique phosphopeptides from 1,087 unique phosphoproteins were i

165 bserved in cancer, we quantified 7566 unique phosphopeptides from 3279 proteins.

166 SI/MS)) can broadly baseline-resolve variant phosphopeptides from a biologically modified human prote

167 tem was applied for the enrichment of casein phosphopeptides from a simulated tryptic digest with bov

168 The nanoparticles enrich phosphopeptides from casein variants, nonfat milk, egg y

169 e protocols to enrich for subfemtomole-level phosphopeptides from cell line and human tissue samples

170 be developed to characterize HLA-associated phosphopeptides from clinical samples that are limited b

171 gated for the enrichment and purification of phosphopeptides from digested protein mixture solutions.

172 e been demonstrated by effectively enriching phosphopeptides from digests of phosphoprotein (alpha- o

173 affinity capture and identification of 3,908 phosphopeptides from fractionated whole-heart homogenate

174 lipovitellin from egg yolk and phospholipids/phosphopeptides from human serum.

175 nia platform for the selective extraction of phosphopeptides from peptide mixtures.

176 Several methods were employed for isolating phosphopeptides from proteolytically digested plasma mem

177 ses is carried out for the identification of phosphopeptides from serum digest and HeLa cell extract.

178 e rounds to enable the maximum extraction of phosphopeptides from the whole cell lysates.

179 e is applied for the selective enrichment of phosphopeptides from tryptic digest of standard protein

180 This facilitates the separation of phosphopeptides from unmodified acidic peptides.

181 ty, the composite was employed for enriching phosphopeptides from yeast protein digests.

182 otypes comprising 14,165 proteins and 18,405 phosphopeptides (from 4,511 proteins), quantified across

183 ides, and for the synthesis of, for example, phosphopeptides, glycopeptides, and N-methylated peptide

184 We found that 50 different phosphopeptides had their phosphorylation state signific

185 At a pH higher than 5, phosphopeptides have two negative charges per residue an

186 e, this is the first report to use SAIFA for phosphopeptide identification.

187 From 8,075 unique phosphopeptides identified, we observe that aberrant act

188 However, water molecules interact with the phosphopeptide in the hydrophilic region of the lattice.

189 sphatase domain that confers specificity for phosphopeptides in a specific sequence context.

190 Cancer cells display novel phosphopeptides in association with MHC class I and II m

191 ania platform can also be used for detecting phosphopeptides in cancer cells (HeLa cells), where it s

192 al of ultra-low flow ESI for the analysis of phosphopeptides in liquid based separation techniques.

193 of phosphoangiotensin and recover 70% of the phosphopeptides in mixtures with a 15-fold excess of non

194 oach for sequentially uncaging two different phosphopeptides in one system, enabling interrogation of

195 rospray ionization (ESI) for the analysis of phosphopeptides in proteomics was investigated.

196 oproteomics to compare the global changes in phosphopeptides in WT and snrk2.2/2.3/2.6 triple mutant

197 Typical methods for enriching phosphopeptides include immobilized metal affinity chrom

198 Analysis of the 273 phosphopeptides increased by dDAVP showed a predominance

199 Whereas all functional phosphopeptides interact with a common phosphate binding

200 ner proteins usually bind via insertion of a phosphopeptide into an amphipathic groove of 14-3-3.

201 We conclude that chromatography of phosphopeptides is best performed at low pH in the ERLIC

202 An expanded library of 967 unique phosphopeptides is detected using significantly reduced

203 ng them through immunization of animals with phosphopeptides is expensive, laborious, and time-consum

204 Collision-induced dissociation (CID) of phosphopeptides leads to the loss of phosphoric or metap

205 f-of-concept 10-plex experiment, we compared phosphopeptide levels from five murine brains to five li

206 We have applied FAIMS to the analysis of a phosphopeptide library comprising the sequences GPSGXVpS

207 yme binding and activity with a microarrayed phosphopeptide library printed on chips coated with eith

208 Using a phosphopeptide library revealed that the score threshold

209 HPLC-coupled phosphopeptide mapping and mass spectrometric analyses i

210 Phosphopeptide mapping by mass spectrometry indicates an

211 ncation analysis, site-directed mutagenesis, phosphopeptide mapping, and phosphoamino acid analysis,

212 s, coupled with phosphorylation analysis and phosphopeptide mapping, to identify Ser-45 and Ser-46 of

213 ion analysis, site-directed mutagenesis, and phosphopeptide mapping, we identified Ser-677, Ser-769,

214 normal as assayed by in vitro kinase and MT phosphopeptide mapping.

215 ry identified 14,155 spectra of 3,371 unique phosphopeptides mapping to 1,159 proteins (false discove

216 ted phosphopeptide fragments with NDAT Ser-7 phosphopeptide markers.

217 The major components of the pipeline include phosphopeptide meta-analysis, correlation network analys

218 al challenge to analysis is the formation of phosphopeptide-metal ion complexes during LC which hampe

219 We are developing phosphopeptide mimetics targeting the SH2 domain of STAT

220 ryptic digest of bovine serum albumin, and a phosphopeptide mixture.

221 lculated Cscore from a large data set (>7000 phosphopeptide MS/MS spectra) was approximately 32 compa

222 e of pY-MIP for enrichment and sequencing of phosphopeptides obtained by tryptic digestion of protein

223 iron fortified goat and cow milks and casein phosphopeptides obtained from each species of milk was d

224 itors that disrupt the interaction between a phosphopeptide of SLAM (signaling lymphocytic activation

225 Characteristic phosphopeptides of phosphoproteins are identified from h

226 identification of over 10,000 unique "basic" phosphopeptides of which many represent putative targets

227 ntially expressed proteins and 11 543 unique phosphopeptides, of which 80% are novel and 7% preferent

228 Polar analytes, including neurotransmitters, phosphopeptides, oligonucleotides, illicit drugs, and ph

229 We also cataloged 144 unique phosphopeptides on known OCT4 interacting partners, incl

230 affect activation of p85/p110beta dimers by phosphopeptides or Gbetagamma.

231 estigated the thermodynamic properties of 18 phosphopeptides or peptide with phosphate mimic and thre

232 We identified distinct phosphopeptide patterns in metastatic tissues compared w

233 We started by comparing the phosphopeptide patterns of cells treated with WNT3A for

234 ve reproducibility by label-free analysis of phosphopeptide peak areas from replicate purifications (

235 Phosphopeptides/phosphoproteins enrichment from biologic

236 Phosphopeptides play a crucial role in many biological p

237 strategies, we succeeded in quantifying 1371 phosphopeptides present in the CE-MS data set and found

238 enables the identification of HLA-associated phosphopeptides presented by human tissue samples contai

239 Selective enrichment of phosphopeptides prior to their analysis by mass spectrom

240 tor interaction between a glycopeptide and a phosphopeptide produces sPGPs that form nanoparticles, w

241 to which phosphate neutral loss occurs from phosphopeptide product ions due to the infrared photoact

242 imensional thin layer chromatography tryptic phosphopeptide profiles suggested that Ser-395 was targe

243 f phosphopeptides and provide high intensity phosphopeptide protonated molecular ion signal intensiti

244 terization of commonalities among identified phosphopeptides provides insights into the specificities

245 r2 >/= 0.80) and high-fidelity (>90% purity) phosphopeptide purification in a 96-well format.

246 the well-established SCX-TiO(2) workflow for phosphopeptide purification on a proteome-wide scale.

247 on interference, we assessed the accuracy of phosphopeptide quantitation across a variety of experime

248 otope-assisted mass spectrometric methods of phosphopeptide quantitation to characterize proteins in

249 Forkhead-associated (FHA) domains are phosphopeptide recognition modules found in many signali

250 FHA domains are phosphopeptide recognition motifs that specifically reco

251 richment demonstrates reproducible synthetic phosphopeptide recovery across 2 orders of magnitude, "w

252 d SWATH-MS and benchmarked using a synthetic phosphopeptide reference data set and phosphopeptide-enr

253 ptides showed large sequence homology in the phosphopeptides released by tryptic hydrolysis and simul

254 RTK phosphopeptides relieve inhibition by nSH2 and cSH2 usin

255 of the Dok7 PH-PTB domains in complex with a phosphopeptide representing the Dok7-binding site on MuS

256 The comparison showed significantly improved phosphopeptide sensitivity in equal sample load and equa

257 s in human CD8 T cells imparted high-avidity phosphopeptide-specific recognition and cytotoxic and cy

258 ed a peptide esterification step to increase phosphopeptide specificity from these low-input samples.

259 ow that AI-ETD can identify 24,503 localized phosphopeptide spectral matches enriched from mouse brai

260 hoproteomics of NFTs clearly identified NF-M phosphopeptides SPVPKS*PVEEAK, corresponding to Ser685,

261 ides from caseins, bovine serum albumin, and phosphopeptide standards.

262 Two phosphopeptides, termed pIRS-21097-1105 and pCDC25b38-46

263 limited repeatability and linearity for most phosphopeptides tested, and different phosphopeptides we

264 involved the microinjection of a fluorescent phosphopeptide that is hydrolyzed specifically by PTPs.

265 t TiO2 enrichment does not fully recover the phosphopeptides that are not identified with the corresp

266 However, phosphopeptides that are unique to IMAC enrichment showe

267 The phosphopeptides that are unique to IMAC enrichment, uniq

268 Cortical phosphopeptides that distinguished female and male CRF-O

269 Binding studies with phosphopeptides that mimic the RNAPII C-terminal domain

270 es demonstrated that this protocol retrieved phosphopeptides that tend to be smaller (<24 residues),

271 Notably, phosphopeptides that were more abundant in female CRF-OE

272 With 10 measurements recorded for each phosphopeptide, this equates to more than 628000 binary

273 ir activities are further enhanced by an RTK phosphopeptide to levels markedly exceeding that of acti

274 he intermediate oxazoline ring formed in the phosphopeptide to the metal-phosphate complex.

275 Exposure of DEACM- and NPE-caged phosphopeptides to 420 nm light selectively releases the

276 s present in the CE-MS data set and found 49 phosphopeptides to be differentially regulated in the tw

277 ficient protection against phosphate loss in phosphopeptides upon collision-induced dissociation tand

278 ratio of (18)O- versus (16)O-labeled tryptic phosphopeptide using high mass accuracy mass spectrometr

279 The phosphopeptide was shown to self-assemble into semi-elli

280 binding affinities of Ga(3+) and Fe(3+) for phosphopeptides, we designed a metal-directed immobilize

281 ized metal affinity chromatography to enrich phosphopeptides, we extracted 849 uniquely identified ph

282 Phosphopeptides were adsorbed onto the amine-functionali

283 r relative and absolute quantitation-labeled phosphopeptides were analyzed by liquid chromatography-t

284 Enriched phosphopeptides were analyzed by Orbitrap tandem mass sp

285 whereas only 10% of 5069 monophosphorylated phosphopeptides were commonly enriched in both fractions

286 de enrichment and purification process, only phosphopeptides were enriched and separated from the oth

287 Phosphopeptides were enriched from 0.5 mg of total pepti

288 r most phosphopeptides tested, and different phosphopeptides were found to have different linear rang

289 Several thousand phosphopeptides were identified after TiO2 enrichment, a

290 erties and resolution in MALDI-TOF-MS, these phosphopeptides were identified as suitable markers for

291 However, only about 60% as many phosphopeptides were identified with SAX at pH 6 than vi

292 In one ERLIC run, 12467 phosphopeptides were identified, including 4233 with mor

293 A total of 28 phosphopeptides were observed by this method.

294 col in which the selectivity and recovery of phosphopeptides were optimized by assessing a twenty-poi

295 In addition, up to 92% of the 6283 phosphopeptides were uniquely enriched in either the fir

296 th IMAC and TiO2 enriched similar amounts of phosphopeptides with comparable enrichment efficiency.

297 he IMAC and TiO2 procedures clearly enriched phosphopeptides with different motifs.

298 e present a straightforward method to enrich phosphopeptides with multiple basic residues, an under-r

299 ue allowed the identification of 2560 unique phosphopeptides with only 8% overlap.

300 identified and quantified over 13,000 unique phosphopeptides, with a large percentage dependent on Sy