ライフサイエンスコーパス: phosphoryl

1 enosine and the oxidized abasic sites, 5'-(2-phosphoryl-1,4-dioxobutane) (DOB) and the C4-hydroxylate

2 The oxidized abasic lesion 5'-(2-phosphoryl-1,4-dioxobutane) (DOB) is an electrophilic pr

3 The oxidized abasic lesion 5'-(2-phosphoryl-1,4-dioxobutane) (DOB) is produced concomitan

4 template-directed primer extension using 5'-phosphoryl-2-methylimidazole-activated nucleotides (2-Me

5 our structures, indicating possible roles in phosphoryl acceptor positioning or catalysis.

6 ed dCK in complex with ACV at the nucleoside phosphoryl acceptor site and UDP at the phosphoryl donor

7 t)/K(m) values in the presence of saturating phosphoryl acceptor with the second order rate constant

8 In this assay, PLK-peptide was chosen as the phosphoryl acceptor.

9 conclusions were drawn when LRRKtide was the phosphoryl acceptor.

10 nd purine nucleosides (and their analogs) as phosphoryl acceptors, dCK can utilize either ATP or UTP

11 ant in all Pcls, acts as a surrogate for the phosphoryl adduct of the phosphorylated, fully activated

12 otein is crystallized in the presence of the phosphoryl analog BeF(3)(-), while the alpha1/alpha 5 di

13 X complexed with its CheY3 substrate and the phosphoryl analogue reveals a binding orientation betwee

14 We conducted a phosphoryl and functional mapping of both structural for

15 n, implying that the ribozyme catalyzes both phosphoryl and nucleotidyl transfers.

16 Understanding phosphoryl and sulfuryl transfer is central to many bioc

17 erved as key model systems for understanding phosphoryl and sulfuryl transfer reactions, respectively

18 nent reaction of alkyne, azides (sulfonyl or phosphoryl azides), and N,N-dialkyloxyformamide dialkyl

19 affinity-based methods identified decaprenyl-phosphoryl-beta-D-ribofuranose oxidoreductase DprE1 and

20 ox that clamps the nucleotide base, a buried phosphoryl binding site, and three solvent-filled pocket

21 tures show that the core domain supplies the phosphoryl binding site, catalytic histidine (His17), an

22 ncreased electron-acceptor properties of the phosphoryl-bridged bipyridine precursor, N-benzylation w

23 We report a structure-property study on phosphoryl-bridged viologen analogues with a remarkably

24 erve agent analogues containing the relevant phosphoryl centers found in GB, GD, GF, VX, and VR has b

25 ing 5-trifluoromethylthiophene derivative by phosphoryl chloride in refluxing pyridine.

26 with isopropylformate, and dehydration with phosphoryl chloride provides an efficient, direct synthe

27 Along with selective production of IgM anti-phosphoryl choline, these data suggest that human B-1 ce

28 with exonic splicing enhancers can regulate phosphoryl content in the RS domain.

29 tein phosphatase PP1, thereby regulating the phosphoryl content of the RS domain.

30 aration and chemistry of novel sulfonyl- and phosphoryl-derived lambda(3)-iodanes are reported.

31 ctivity using phosphorylated rPleCHKD as the phosphoryl donor but not with two other recombinant hist

32 Protein kinases use ATP as a phosphoryl donor for the posttranslational modification

33 e-6-phosphate production, utilizing ADP as a phosphoryl donor in contrast to the more well characteri

34 The phosphoryl donor in the catalytic cleft of alpha-D-phosp

35 side phosphoryl acceptor site and UDP at the phosphoryl donor site.

36 ally relevant small molecule, can serve as a phosphoryl donor to a subset of two-component response r

37 All ACKs utilize ATP/ADP as the phosphoryl donor/acceptor in the respective directions o

38 eptors, dCK can utilize either ATP or UTP as phosphoryl donors.

39 phate compartmentation with normal ATP gamma-phosphoryl dynamics.

40 c assay for the ultratrace quantitation of a phosphoryl fluoride nerve agent surrogate.

41 e biotin modified hairpin probe (HP) with 3'-phosphoryl, forming multifunctional magnetic probes (HP-

42 Although the presence of a 3'-phosphoryl group abolishes exoribonuclease action, it ha

43 idal, as were PhoB binding isotherms for the phosphoryl group analog BeF3(-).

44 of the five CheY mutants (complexed with the phosphoryl group analogue BeF(3)(-)) to wild-type CheY o

45 le making contacts solely to the transferred phosphoryl group and its incoming and outgoing atoms.

46 approach, the putative roles of the Thr(160) phosphoryl group and the T-loop conformation were invest

47 interactions between AP and the transferred phosphoryl group are not present in the ground state des

48 main response regulator CheY2-P shuttles its phosphoryl group back to CheA, while a second response r

49 he reversible Mg(2+)-dependent transfer of a phosphoryl group between ATP and taurocyamine.

50 small-molecule metabolite that can act as a phosphoryl group donor for response regulators of two-co

51 and showed that acetyl-phosphate (AcP) is a phosphoryl group donor to RcsB.

52 active site residue in stabilizing the donor phosphoryl group during catalysis.

53 PGK1) catalyzes the reversible transfer of a phosphoryl group from 1, 3-bisphosphoglycerate (1, 3-BPG

54 ified deoxyribozymes that transfer the gamma-phosphoryl group from a 5'-triphosphorylated donor (a pp

55 yze the reversible transfer of a high-energy phosphoryl group from ATP to l-arginine to form phosphoa

56 Transfer of a phosphoryl group from autophosphorylated CheA (P-CheA) t

57 ermediate protein involved in transferring a phosphoryl group from multiple sensor kinases to the res

58 acid phosphatases catalyze the transfer of a phosphoryl group from phosphomonoesters to water at acid

59 trong evidence for steric obstruction of the phosphoryl group from the attacking water molecule as on

60 domain (the "P1" domain) of CheA receives a phosphoryl group from the catalytic domain (P4) of CheA

61 domain of P-CheA and then (ii) acquiring the phosphoryl group from the P1 domain.

62 two-component systems occurs by loss of the phosphoryl group from the response regulator protein.

63 The documented role of an amide moiety in phosphoryl group hydrolysis suggests an analogous cataly

64 he axial O-Mg-O alignment for the TSA of the phosphoryl group in the catalytic site differ by only ap

65 state despite the apparent similarity of the phosphoryl group in the ground and transition states.

66 e for specific and strong recognition of the phosphoryl group in the transition state.

67 e third metal ion stabilizes the transferred phosphoryl group in the transition state.

68 The surface of CheY3 containing the phosphoryl group interacts directly with a long helix of

69 The phosphoryl group is subsequently transferred to an aspar

70 The phosphoryl group is subsequently transferred to cytosoli

71 ryl lipid A, indicating a change in a single phosphoryl group is sufficient for TRIF-biased TLR4 stim

72 From H2, the phosphoryl group is transferred to D2 on the response re

73 sponse regulator heterodimers containing one phosphoryl group may participate in gene regulation.

74 covering that the enzyme transfers the gamma-phosphoryl group of ATP to the E270 actin residue, resul

75 ain and then catalyzes transfer of the gamma-phosphoryl group of ATP to the His(45) side chain within

76 eversible phosphoryl group transfer of the N-phosphoryl group of phosphoglycocyamine to ADP to yield

77 a water molecule for the attack on the gamma-phosphoryl group of the nucleotide, stabilization of the

78 P binding, presumably due to the high energy phosphoryl group on the fluorescent probe (ATP.E2 analog

79 tor signaling protein, and hydrolysis of the phosphoryl group reestablishes the inactive state.

80 g water molecule as one mechanism to enhance phosphoryl group stability.

81 se autophosphorylates and then transfers its phosphoryl group to a cognate response regulator.

82 an environmental stimulus and transfers the phosphoryl group to a transcription factor/response regu

83 ding energy of their substrate's nonreacting phosphoryl group to accelerate catalysis.

84 sphoryl transfer protein, HPr, transfers its phosphoryl group to any of several sugar-specific Enzyme

85 ive cytoplasmic domain of Nla28S transfers a phosphoryl group to Nla28 in vitro, that the phosphotran

86 inin treatment, where they then transfer the phosphoryl group to nuclear-localized response regulator

87 atalyze hydrolysis of the response regulator phosphoryl group to terminate signal transduction are po

88 Adding a single phosphoryl group to the preformed RIIbeta holoenzyme thu

89 by autophosphorylating and transferring the phosphoryl group to the response regulator BvgA.

90 (k(cat)) is rate-limited by both reversible phosphoryl group transfer (k(forward) approximately 0.2

91 bly the opening of the activation loop after phosphoryl group transfer but preceding product release.

92 y, catalyzes the Mg(2+)-dependent reversible phosphoryl group transfer of the N-phosphoryl group of p

93 With this assay, we discovered phosphoryl group transfer that labeled CFTR, thereby dem

94 trates is critical to the catalysis of (thio)phosphoryl group transfer, but there has been no systema

95 ause of their inability to transfer the beta-phosphoryl group, and thus enable the distinction betwee

96 electronic polarization of the transferring phosphoryl group, primarily mediated by H-bonding to O(3

97 ing group to the nicotinamide-N1 while the 5-phosphoryl group, the pyrophosphate moiety, and the nico

98 dinated to a nonbridging oxygen of the gamma-phosphoryl group.

99 a ketone carbonyl, a nitrile, an ester, or a phosphoryl group.

100 n of the Thr64 nucleophile and the substrate phosphoryl group.

101 nvironment complementary to the transferring phosphoryl group.

102 by the presence of a 3'-CCA sequence or a 3'-phosphoryl group.

103 e and tetrafluoroaluminate surrogates of the phosphoryl group.

104 transiently bind and transfer signal, i.e., phosphoryl group.

105 tions, the SLN1 sensor kinase is active, and phosphoryl groups are shuttled through YPD1 to SSK1, the

106 The results show that the beta- and alpha-phosphoryl groups are transferred either directly or ind

107 In the PTS, phosphoryl groups are transferred from phosphoenolpyruva

108 nsidering interactions of amide, sulfur, and phosphoryl groups associated to proteins from bacteria o

109 n after phosphorylation shows that the added phosphoryl groups can prime vinculin for activation.

110 p53 phosphorylation; addition of successive phosphoryl groups enhances the affinity for the TAZ1, TA

111 and Hpt3 are likely the dominant sources of phosphoryl groups for PilG and PilH, respectively.

112 tilis is governed by a phosphorelay in which phosphoryl groups from a histidine kinase are successive

113 gulator, CheY1, serves as a sink for surplus phosphoryl groups from CheA-P.

114 The labile nature of phosphoryl groups has presented a long-standing challeng

115 iated switches within RRs, but some transfer phosphoryl groups in multistep phosphorelays.

116 he truncated protein was competent to accept phosphoryl groups in trans.

117 The enzymatic transfer of phosphoryl groups is central to the control of many cell

118 observed that electrostatic repulsion of 5'-phosphoryl groups promoted the formation of aggregates i

119 f Spo0A P due to fluctuations in the flux of phosphoryl groups through the phosphorelay.

120 9 kinases autophosphorylated and transferred phosphoryl groups to Spo0A in vitro, confirming their ro

121 hatases that negatively modulate the flow of phosphoryl groups to Spo0A.

122 HKs transfer phosphoryl groups to their specific RRs, but also dephos

123 The insertion of phosphoryl groups varied to a large extent depending on

124 ions accumulate to a greater extent near the phosphoryl groups, penetrating deeper into the grooves.

125 idate diastereochemistry (D-/L-alanine, R-/S-phosphoryl) in vitro and in vivo.

126 dystrophy), a group of enzymes with apparent phosphoryl-ligand transferase activity that are found in

127 We show here DDK-phosphoryled Mcm2 preferentially interacts with Cdc45 in

128 These results suggest that phosphoryl moieties of LA from N. meningitidis and N. go

129 sociated molecular patterns by expression of phosphoryl moieties on the LA to optimize interactions w

130 To transmit an activated phosphoryl moiety from the Ac-CoA binding site (within t

131 gested as a hydrogen bonding partner for the phosphoryl moiety of GMP.

132 The replacement of the pro-S oxygen in the phosphoryl moiety of PI by sulfur results in a 3 x 10(7)

133 Then, in the presence of T4 PNK, the 3'-phosphoryl of HP-MBs was hydrolyzed to 3'-hydroxyl, thus

134 communication but increased dynamics of beta-phosphoryls of ADP/ATP, G-6-P and gamma-/beta-phosphoryl

135 ygens: two water molecules, the ss and gamma phosphoryls of GTP, a helix-alpha1 Ser, and a switch I d

136 hosphoryls of ADP/ATP, G-6-P and gamma-/beta-phosphoryls of GTP, indicating redistribution of flux th

137 ides (MFx ) as ligands that imitate either a phosphoryl or a phosphate group was 357 at the end of 20

138 ecule remarkably plays a similar role to the phosphoryl or aspartate group.

139 Ribozymes can catalyze phosphoryl or nucleotidyl transfer onto ribose hydroxyls

140 enabled through a hydrogen bond between the phosphoryl oxygen and the aldehyde formyl proton present

141 e pseudoaxial cyclic boronate oxygen and the phosphoryl oxygen interacts with the formyl proton.

142 onate and a stabilizing interaction from the phosphoryl oxygen of the catalyst to the formyl hydrogen

143 .0228 and (15)k = 1.0014, at the nonbridging phosphoryl oxygens (18)k(nonbridge) = 0.9954, and at the

144 rate-bound state of 2:1a or 3:1a has the two phosphoryl oxygens bridging Zn((II))1 and Zn((II))2.

145 leophile, 5'O leaving group, and nonbridging phosphoryl oxygens for RNase A to values observed for hy

146 static interactions to the Raman spectrum of phosphoryl oxygens have not been analyzed quantitatively

147 izing the negative charge on the nonbridging phosphoryl oxygens.

148 o optically active alpha-sulfonyl- and alpha-phosphoryl oxyketones in respectable yields and enantios

149 m of enzymes that manipulate the transfer of phosphoryl (PO3(-) ) groups.

150 dpiece domains and which is essential to the phosphoryl regulation of dematin headpiece.

151 The phosphoryl-rich character of these anions was designed t

152 y studies on exon1-like molecules containing phosphoryl-Ser residues at positions 13 and 16 show that

153 However, since no analogous 1,3-phosphoryl shift is operational, N-phosphoryl ynamides c

154 s activated only when NepR and an activating phosphoryl signal are present.

155 The span between the hydrophobic box and the phosphoryl site is optimal for recognizing nucleoside mo

156 arise from distinct interactions at a single phosphoryl substituent.

157 rometry that are indicative of the number of phosphoryl substituents on the lipid A (LA) component of

158 s, leading to hitherto unknown sulfonyl- and phosphoryl-substituted phosphinolines, phosphininothioph

159 lls treated with 89I LOS, which had the most phosphoryl substitutions on the LA compared with 1291 LO

160 signaling in a manner that is independent of phosphoryl transfer (classical pseudokinases; noncanonic

161 monas aeruginosa catalyzes an intramolecular phosphoryl transfer across its phosphosugar substrates,

162 ubiquitous four-domain enzyme that catalyzes phosphoryl transfer across phosphohexose substrates.

163 Investigation of the Mg(2+) requirements for phosphoryl transfer activity of IRAK-4 revealed that mor

164 g(2+) ions play a crucial role in regulating phosphoryl transfer and can limit overall enzyme turnove

165 h their effects on positioning reactants for phosphoryl transfer and easing barriers to transcript ba

166 Phosphoryl transfer and proofreading hydrolysis are cont

167 apply this understanding to enzyme-catalyzed phosphoryl transfer and provide illustrative examples of

168 roles: a direct role in the chemical step of phosphoryl transfer and secondly through propagation of

169 Despite the central importance of phosphoryl transfer and the fascinating catalytic challe

170 , the effects of the phosphorylations on the phosphoryl transfer are smaller.

171 bipyramidal transition states formed during phosphoryl transfer are stabilized by electrostatic forc

172 Phosphoryl transfer assays in vitro confirmed that these

173 te-directed mutagenesis and various in vitro phosphoryl transfer assays using cyclic AMP-dependent pr

174 systems typically entails an intermolecular phosphoryl transfer between a sensor kinase (SK) and a c

175 d activity in catalysis of the Asp8-mediated phosphoryl transfer between betaG1,6bisP and betaG1P but

176 complex enforces an asymmetric mechanism of phosphoryl transfer between ChpT and CtrA.

177 Here we report on the kinetics of phosphoryl transfer between different sensor-regulator c

178 ow that the beta3-lysine is not required for phosphoryl transfer but is essential for the active stat

179 tant contribution of anionic nucleophiles to phosphoryl transfer catalysis via ground state electrost

180 MM) approach, we propose a mechanism for the phosphoryl transfer catalyzed by ERK that offers new ins

181 ylation of its numerous substrates through a phosphoryl transfer chain where a phosphoryl transfer pr

182 combinases catalyze DNA rearrangements using phosphoryl transfer chemistry that is identical to that

183 relationship of this novel calcium-activated phosphoryl transfer enzyme.

184 ely reported trigonal AlF(3)(0) complexes of phosphoryl transfer enzymes may have been misassigned an

185 Among well-characterized phosphoryl transfer enzymes, hSCAN-1 is unique not only

186 alance in transition-state stabilization for phosphoryl transfer enzymes.

187 rove helpful in mechanistic studies of other phosphoryl transfer enzymes.

188 g a conceptual link among these multidomain, phosphoryl transfer enzymes.

189 smaller change in the free energy barrier of phosphoryl transfer found by QM/MM simulations.

190 llowing the ribozyme to radiolabel itself by phosphoryl transfer from [gamma-(32)P]GTP, DNAzyme-media

191 rial RNA repair system, catalyzes reversible phosphoryl transfer from a nucleoside triphosphate (NTP)

192 Phosphomevalonate kinase (PMK) catalyzes phosphoryl transfer from adenosine triphosphate (ATP) to

193 rial RNA repair system, catalyzes reversible phosphoryl transfer from an NTP donor to a 5'-OH polynuc

194 ureus exogenous fatty acids are activated by phosphoryl transfer from ATP to form acyl-phosphates, a

195 yr to the catalytic Asp1132, followed by the phosphoryl transfer from ATP to substrate Tyr.

196 p8 of the core domain active site to mediate phosphoryl transfer from beta-glucose 1,6-(bis)phosphate

197 Phosphoryl transfer from GTP is greatly reduced in the a

198 The open conformation of apo EI allows phosphoryl transfer from His189 located in the N-termina

199 the 98 Da neutral loss occurs via gas-phase phosphoryl transfer from pHis to the peptide C-terminal

200 vert betaG1P to G6P, and an enhanced rate of phosphoryl transfer from phospho-Asp8 to water.

201 ion domain (EIC), thereby permitting in-line phosphoryl transfer from phosphoenolpyruvate (PEP) bound

202 e presence of a second, albeit unproductive, phosphoryl transfer in ACL.

203 e closed conformation, observed in a trapped phosphoryl transfer intermediate, brings the EIN(alpha/b

204 However, covalent phosphoryl transfer is not completed, and no catalytic t

205 ct release is rate-limiting for LRRKtide and phosphoryl transfer is rate-limiting for LRRKtide(S).

206 These results indicate that phosphoryl transfer is reversible and that a slow kineti

207 This intermolecular phosphoryl transfer is seemingly counter to what is anti

208 that AP endo acts by a one-step associative phosphoryl transfer mechanism on a THF-containing substr

209 One of the phosphoryl transfer mechanisms, that of acetate kinase,

210 strate specificity and overhangs the site of phosphoryl transfer near the water-membrane interface.

211 ) the I2020T mutant accelerates the rates of phosphoryl transfer of both reactions by 3-7-fold; (ii)

212 e we identified conditions that yielded slow phosphoryl transfer of the gamma-phosphate from the gene

213 miting conformational change step before the phosphoryl transfer of the incoming nucleotide to the pr

214 f the disaccharide-1-phosphate substrate for phosphoryl transfer on the inner membrane.

215 Phosphoryl transfer onto backbone hydroxyls is a recogni

216 -base chemistry, had little effect on either phosphoryl transfer or proofreading hydrolysis by Escher

217 Phosphoryl transfer plays a central role in many biochem

218 A phosphoryl transfer protein of the PTS, NPr, homologous

219 through a phosphoryl transfer chain where a phosphoryl transfer protein, HPr, transfers its phosphor

220 lected in a 20-fold decrease in the apparent phosphoryl transfer rate as measured by pre-steady-state

221 duct complexes complete the snapshots of the phosphoryl transfer reaction by PKAc, providing us with

222 States along the phosphoryl transfer reaction catalyzed by the nucleoside

223 The hairpin ribozyme accelerates a phosphoryl transfer reaction without catalytic participa

224 he nucleophilic water, and to facilitate the phosphoryl transfer reaction.

225 hird metal ion was shown to be essential for phosphoryl transfer reaction.

226 es play a critical role in several enzymatic phosphoryl transfer reactions and have been studied exte

227 the uncatalyzed reactions that correspond to phosphoryl transfer reactions catalyzed by kinases and t

228 srupt transesterification steps of important phosphoryl transfer reactions in DNA and RNA.

229 promote one class of biologically important phosphoryl transfer reactions in DNA, exemplify active s

230 ctive site mechanisms that promote important phosphoryl transfer reactions in nucleic acids.

231 cation, but the detailed mechanisms of these phosphoryl transfer reactions remain elusive.

232 leic acid metabolizing enzymes that catalyze phosphoryl transfer reactions using two divalent metal i

233 developed semiempirical AM1/d-PhoT model for phosphoryl transfer reactions.

234 sfer, decarboxylation, hydride transfer, and phosphoryl transfer reactions.

235 For most kinases, the phosphoryl transfer step is highly efficient, while the

236 single proton transfer in the rate-limiting phosphoryl transfer step.

237 the phenolate moiety as a nucleophile in the phosphoryl transfer step.

238 o D-glucose 6-phosphate (G6P) via sequential phosphoryl transfer steps using a beta-D-glucose 1,6-bis

239 denoting similar transition states for both phosphoryl transfer steps.

240 for all four sugar branches of the bacterial phosphoryl transfer system (PTS).

241 neate a reaction pathway for ErbB3-catalyzed phosphoryl transfer that does not require the conserved

242 stem, a signal transduction pathway in which phosphoryl transfer through a series of bimolecular prot

243 Our study delineates phosphoryl transfer through this molecular pathway, whic

244 f a sensor histidine kinase (HK) followed by phosphoryl transfer to a cognate response regulator (RR)

245 ulting in autophosphorylation and subsequent phosphoryl transfer to a response regulator (EutV) conta

246 s a signal transduction pathway that couples phosphoryl transfer to active sugar transport across the

247 e energy landscape, which in turn allows the phosphoryl transfer to occur selectively by avoiding sid

248 fined to 2.15 A resolution displays complete phosphoryl transfer to the substrate.

249 alytically active conformation, facilitating phosphoryl transfer to the substrate.

250 tes but mirrors the geometry of the presumed phosphoryl transfer transition state.

251 e an ideal transition state analog for loose phosphoryl transfer transition states.

252 We confirmed intermolecular phosphoryl transfer using an isotope relay assay in whic

253 emains in the active site following complete phosphoryl transfer while Mg1 is expelled.

254 tions serve to precisely arrange the site of phosphoryl transfer within the complex.

255 Compared with nonenzymatic phosphoryl transfer, all three kinases were found to pro

256 binding site, the key residues in catalyzing phosphoryl transfer, and the substrate specificity diffe

257 otein kinases, the enzymes that catalyze the phosphoryl transfer, are implicated in practically every

258 otein kinases, the enzymes that catalyze the phosphoryl transfer, are involved in nearly every aspect

259 hly conserved beta3-lysine was essential for phosphoryl transfer, but our findings show that the beta

260 Following phosphoryl transfer, Mg2 recruits a water molecule to re

261 hairpin ribozyme plays an important role in phosphoryl transfer, possibly functioning as a general a

262 ermodynamic and kinetic data for the initial phosphoryl transfer, subsequent hydrolysis, and finally,

263 f two Mg(2+) ions is essential for efficient phosphoryl transfer, the presence of both Mg(2+) ions in

264 ltiple studies of PKA, the steps involved in phosphoryl transfer, the roles of the catalytically esse

265 route constructs the pyrrolophane motif via phosphoryl transfer-terminated macroaldolization and pas

266 he rejection of incorrect nucleotides before phosphoryl transfer.

267 ring with the bending of the P-domain during phosphoryl transfer.

268 binding of two Mg(2+) ions for catalysis of phosphoryl transfer.

269 mational changes, thereby facilitating rapid phosphoryl transfer.

270 hich are reported to catalyze intramolecular phosphoryl transfer.

271 transition state dominates enzyme-catalyzed phosphoryl transfer.

272 rotonation of the 3'-OH group and subsequent phosphoryl transfer.

273 y steps in the catalytic cycle leading up to phosphoryl transfer.

274 ositioning the glycine-rich loop and ATP for phosphoryl transfer.

275 h the glycine-rich loop that is required for phosphoryl transfer.

276 the sulfur atom is positioned for an in-line phosphoryl transfer.

277 bunits too distant ( approximately 14 A) for phosphoryl transfer.

278 se shows several similarities in the site of phosphoryl transfer: 1) preservation of architecture of

279 imic "in-line" anionic transition states for phosphoryl transfer; and 3) trigonal bipyramidal complex

280 Thus, Ire1-catalyzed phosphoryl-transfer aids disassembly of Ire1 signaling c

281 osed domain conformation, allowing efficient phosphoryl-transfer catalysis.

282 Adenylate kinase (AdK) is a phosphoryl-transfer enzyme with important physiological

283 Phosphoryl-transfer reactions are central to biology.

284 al data are available for rates of enzymatic phosphoryl-transfer reactions in the absence of the diva

285 Here we review phosphoryl-transfer reactions with the goal of providing

286 Ribozymes, which carry out phosphoryl-transfer reactions, often require Mg(2+) ions

287 human Ire1alpha bound to ADP, revealing the 'phosphoryl-transfer' competent dimeric face-to-face comp

288 Protein kinase A (PKA) is a ubiquitous phosphoryl transferase that mediates hundreds of cell si

289 alytic modes and active site environments of phosphoryl transferases influence transition state struc

290 as mobile packets of cellular currencies for phosphoryl transfers (ATP), acyl transfers (acetyl-CoA,

291 provide new insights into histidyl-aspartyl phosphoryl transfers in two-component systems and sugges

292 ium fluoride (E2.BeF) as analogs of the E2.P phosphoryl transition state and E2P ground state, respec

293 A His89-P-His-15 pentacoordinate phosphoryl transition state can readily be modeled witho

294 Chb) is inconsistent with the formation of a phosphoryl transition state intermediate because of ster

295 Ca(2+)-ATPase, the ATP affinity of the E2.P phosphoryl transition state is higher than that of the E

296 le with the formation of the His-89-P-Cys-10 phosphoryl transition state without necessitating any ch

297 horylation reflects a destabilization of the phosphoryl transition state.

298 tighten the interaction with ATP in the E2.P phosphoryl transition state.

299 ogous 1,3-phosphoryl shift is operational, N-phosphoryl ynamides could be used to prepare similar cyc

300 ilic bis-silyl ynamines and N-sulfonyl and N-phosphoryl ynamides serve as the reaction partner in the