ライフサイエンスコーパス: phosphothreonine

1 g pKa values of 5.6 (phosphoserine) and 5.9 (phosphothreonine).

2 tified 32P-Tyr, [32P]phosphoserine, and [32P]phosphothreonine.

3 ree P-Tyr (5 mM) but not by phosphoserine or phosphothreonine.

4 residue but did not cleave phosphoserine or phosphothreonine.

5 many but not all cases specifies binding to phosphothreonine.

6 zen polypeptides, several of which contained phosphothreonine.

7 strates because B55 displays selectivity for phosphothreonine.

8 dition of phosphotyrosine, phosphoserine, or phosphothreonine.

9 and PH domains, respectively, bind ICP10 at phosphothreonines 117 and 141 and a WD40-like motif at p

10 MLCP dephosphorylated phosphoserine 19, phosphothreonine 18, and phosphothreonine 9 efficiently

11 Using a phosphothreonine 187 site-specific antibody for p27, we

12 ing an antibody that specifically recognizes phosphothreonine-231) and T-tau using ultra-high sensiti

13 By detecting phosphothreonine 388 of mosquito S6 kinase, we show that

14 th fetal bovine serum markedly increased the phosphothreonine 410 content of CAT zeta and stimulated

15 A phosphothreonine 423-specific antibody detected phosphor

16 PPM1D dephosphorylation of UNG2 at phosphothreonine 6 is associated with reduced UNG2 activ

17 We hypothesized that the presence of phosphothreonine 753 either renders beta(3) a poor subst

18 d phosphoserine 19, phosphothreonine 18, and phosphothreonine 9 efficiently with almost identical rat

19 Dianionic phosphothreonine induced strong phosphothreonine amide protection and downfield amide ch

20 Forkhead-associated (FHA) domains bind phosphothreonine and control many aspects of cell prolif

21 ot discriminate between dephosphorylation of phosphothreonine and phosphoserine residues in synthetic

22 ytically active form of Cdc25A for efficient phosphothreonine and phosphotyrosine dephosphorylation.

23 The lip is refolded, bringing the phosphothreonine and phosphotyrosine into alignment with

24 ephosphorylate contiguous and semicontiguous phosphothreonine and phosphotyrosine on cyclin dependent

25 activation of the MAP kinases by hydrolyzing phosphothreonine and phosphotyrosine residues present in

26 Alpha-ZrPN also bound peptides containing phosphothreonine and phosphotyrosine.

27 es in immunoblotting with anti-phosphoserine/phosphothreonine antibodies.

28 solution Raman spectra of phosphoserine and phosphothreonine are assigned to the monobasic and dibas

29 sine, but did not hydrolyse phosphoserine or phosphothreonine at a measureable rate.

30 While substitution of phosphothreonine at position 16 causes generally similar

31 nd unusual preference for phosphoserine over phosphothreonine at Pro-directed sites in vitro.

32 due was identified that sterically restricts phosphothreonine binding and is largely responsible for

33 ks the structural properties associated with phosphothreonine binding and thus most likely interacts

34 Phosphoserine- or phosphothreonine-binding activity was required for Pin1

35 d synchronously inactivate all phosphoserine/phosphothreonine-binding domain family members in a rapi

36 WW domain functioned as a phosphoserine- or phosphothreonine-binding module, with properties similar

37 e been well-characterized, phosphoserine- or phosphothreonine-binding modules have not been described

38 ysis of suppressors of a mutation in the Cdk phosphothreonine-binding pocket created by cyclin bindin

39 d Arg294 and Arg295 that likely comprise the phosphothreonine-binding pocket in PAC-1 to either alani

40 N, inactivated Hog1 by dephosphorylating the phosphothreonine but not the phosphotyrosine residue in

41 racts are supershifted by phosphoserine- and phosphothreonine- but not phosphotyrosine-specific antib

42 ecreased phosphoserine by 80%, and decreased phosphothreonine by 70%.

43 ferred substrate, although phosphoserine and phosphothreonine can also be cleaved.

44 ited a comparable substrate preference for a phosphothreonine containing substrate, consistent with t

45 cognition motifs that specifically recognize phosphothreonine-containing epitopes.

46 ntation performs validation of phosphoserine/phosphothreonine-containing peptides having one or two p

47 inhibited Wip1 phosphatase activity, whereas phosphothreonine-containing peptides with the sequence p

48 Phosphorylation of a 130-kDa phosphothreonine-containing protein (FP130) occurred thr

49 One of the phosphothreonine-containing proteins was identified by m

50 Because FHA domains respond to phosphothreonine-containing proteins, these results sugg

51 phatases that hydrolyze phosphotyrosine- and phosphothreonine-containing substrates.

52 Our observations suggest that phosphoserine/phosphothreonine-dependent localization is a key feature

53 horylation site contained a phosphoserine or phosphothreonine followed by a proline, suggesting that

54 In the accompanying paper, we identify three phosphothreonines important in this process.

55 tase 2A (PP2A) in vitro to dephosphorylate a phosphothreonine in a conserved TPNK sequence in the SLB

56 By combining a method to biosynthesize phosphothreonine in cells with this selection approach,

57 ion specificity of EYA1 in dephosphorylating phosphothreonine in Myc but also reveal an important mec

58 carbachol, leads to the rapid appearance of phosphothreonine in nonmuscle myosin heavy chain II-A (N

59 entirely biosynthetic route to incorporating phosphothreonine in proteins.

60 Glutamic acid can substitute for phosphothreonine in some proteins activated by phosphory

61 hat rWip1 dephosphorylates phosphoserine and phosphothreonine in the p(S/T)Q motif, which is an essen

62 eloped by measuring stable phosphoserine and phosphothreonine in vitro.

63 Dianionic phosphothreonine induced strong phosphothreonine amide p

64 ty for phosphotyrosine over phosphoserine or phosphothreonine is considerable, but the enzyme did not

65 exposed yet deep active site cleft while the phosphothreonine is loosely tethered into a nearby basic

66 ss spectrometry we demonstrate that SpvC has phosphothreonine lyase activity on full-length phospho-E

67 ated and that this modification inhibits its phosphothreonine lyase activity.

68 The newly discovered bacterial phosphothreonine lyases perform a post-translational mod

69 ue with a non-hydrolyzable the phosphoserine/phosphothreonine mimetic would promote binding to the 14

70 rylated by a kinase other than MEK1 and that phosphothreonines on Red1 then interact with the Mek1 FH

71 s with hot spots specific for phosphoserine, phosphothreonine or phosphotyrosine.

72 blot analysis using anti-phosphoserine, anti-phosphothreonine, or anti-phosphotyrosine antibody to de

73 ily of Mg2+ -dependent phosphoserine (P.Ser)/phosphothreonine (P.Thr)-specific phosphatases.

74 agrees with that of KI-FHA in contact with a phosphothreonine peptide ligand.

75 ore modeled on natural product inhibitors of phosphothreonine phosphatases, we generated a refined li

76 Pin1 binds specifically to phosphoserine- or phosphothreonine-proline (pS/T-P) motifs in target prote

77 ally recognizes the phosphoserine-proline or phosphothreonine-proline bonds present in mitotic phosph

78 PP2Calpha is a metal-dependent phosphoserine/phosphothreonine protein phosphatase and is the represen

79 Using phosphothreonine, protein kinase A (PKA), and PKC substr

80 its no activity toward phosphoserine (pS) or phosphothreonine (pT) peptides.

81 yeast Rad53p, which is a naturally occurring phosphothreonine (pT)-binding domain, and found it to be

82 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding domain and determined it

83 Phosphothreonine (pThr) was found to constitute a new cl

84 photyrosine alone (pY), or phosphotyrosine + phosphothreonine (pYT).

85 s mediated by an extended surface containing phosphothreonine recognition and hydrophobic interfaces

86 kinase A phosphorylation and the loss of the phosphothreonine residue and a major phosphopeptide that

87 f the enteropathogen Shigella to convert the phosphothreonine residue of the pT-X-pY consensus sequen

88 ally high pK values of the phosphoserine and phosphothreonine residues and the preference for a diani

89 Phosphoserine and phosphothreonine residues exhibited ordered values of (3

90 mination of phosphate from phosphoserine and phosphothreonine residues followed by addition of an aff

91 l protein domains that bind phosphoserine or phosphothreonine residues have been identified, includin

92 phosphate moiety from both phosphoserine and phosphothreonine residues in low-energy collision-induce

93 sphorylates both the phosphotyrosine and the phosphothreonine residues in the activation loop of the

94 chemical transformation of phosphoserine and phosphothreonine residues into lysine analogs (aminoethy

95 h the dephosphorylation of phosphoserine and phosphothreonine residues of the cyclase receptor.

96 addition and conversion of phosphoserine and phosphothreonine residues to S-ethylcysteinyl or beta-me

97 ical derivatization of the phosphoserine and phosphothreonine residues using stable isotopic variants

98 values for the presence of phosphoserine or phosphothreonine residues using tandem mass spectrometry

99 in the production of both phosphoserine and phosphothreonine residues.

100 calorimetry, using peptides containing both phosphothreonine residues.

101 Nup species revealed only phosphoserine and phosphothreonine residues.

102 atase and dephosphorylates phosphoserine and phosphothreonine residues.

103 insert loop with multiple phosphoserine and phosphothreonine residues.

104 FHA domains in other proteins recognize phosphothreonine residues.

105 hosphorylating phosphotyrosine residues over phosphothreonine residues.

106 odies to O-linked GlcNAc, phosphoserine, and phosphothreonine showed that hyperglycemia increased Glc

107 ss-phosphorylating TRPM7 as assessed using a phosphothreonine-specific antibody but not vice versa.

108 g protein) and in BRCA1 as phosphoserine- or phosphothreonine-specific binding modules that recognize

109 T repeats in BRCA1 are phosphoserine- and/or phosphothreonine-specific binding modules.

110 CD0242 protein catalyses the addition of the phosphothreonine to the N-acetylglucosamine moiety and C

111 Phosphothreonine was detected within two TPVK motifs and

112 No Stat5a/b phosphothreonine was detected.

113 oncognate amino acids glutamate, serine, and phosphothreonine without the need for a separate hydroly