ライフサイエンスコーパス: photoactivate

1 mbranes and displays weak fluorescence until photoactivated.

2 e-red light, a condition in which OCP is not photoactivated.

3 revious work has demonstrated the ability of photoactivated 1 to platinate DNA, this suggests that th

4 sed the degree of irreversible inhibition by photoactivated 3-azioctanol.

5 eased the irreversible inhibition induced by photoactivated 3-azioctanol.

6 3-azioctanol and the irreversible effects of photoactivated 3-azioctanol.

7 c framework for formation of adducts between photoactivated 3-hydroxyphenyl azide and RNA.

8 originating from the covalent attachment of photoactivated 4 was identified.

9 of G(1), S, and G(2) cells were treated with photoactivated 4'-hydroxymethyl-4,5',8-trimethylpsoralen

10 istance of control inoculum-treated PBMCs to photoactivated 8-methoxypsoralen- and hypericin-induced

11 th the amount of PS1 that is modifiable by a photoactivated active site-directed gamma-secretase inhi

12 lved these critical concerns by expressing a photoactivated adenylate cyclase that allows light-sensi

13 particular versatility, naturally occurring photoactivated adenylate cyclases promote the synthesis

14 resent a transgenic mouse model expressing a photoactivated adenylyl cyclase (bPAC) in sperm.

15 identified the blaC gene encoding a putative photoactivated adenylyl cyclase in the Beggiatoa sp. PS

16 Photodynamic therapy uses a photoactivating agent to selectively treat choroidal neo

17 Other photoactivating agents are being evaluated.

18 transduction inactivation through binding to photoactivated and phosphorylated rhodopsin (RP).

19 cI molecules in live cells by stochastically photoactivating and observing fluorescent proteins indiv

20 discrete protein population within axons is photoactivated, and then the activated population is tra

21 We found the excitation light photoactivates as well as deactivates Dronpa single mole

22 Indeed, when caged Tbeta4 was locally photoactivated at the wings of locomoting keratocytes, s

23 oactivatable GFP)-tagged neurofilaments from photoactivated axonal segments in cultured dorsal root g

24 eled with a fluorescent probe or linked to a photoactivated bead, respectively.

25 med the appropriate disulfide bond, could be photoactivated but with reduced sensitivity, and underwe

26 When OCP is photoactivated by strong blue-green light, it is able to

27 e dark on the time scale of hours but can be photoactivated by UV light to regenerate YFP(-) within s

28 Fluorescent steroids photoactivated by visible light may be useful for modula

29 The combination of this photoactivated catalyst with the fidelity and functional

30 t Drosophila ligands; alternatively, Rac was photoactivated centrally within a single cell.

31 mbrane photosynthetic protein, in its robust photoactivated charge separation with near unity quantum

32 o damage the 5' guanine of 5'-GG-3' sites by photoactivated charge transport through DNA.

33 resistant to apoptosis induced by the potent photoactivated chemotherapeutic agents 8-methoxypsoralen

34 purine or pyrimidine 12mer are targeted with photoactivated cleavage by the metal complex Rh(phen)2ph

35 ontext, 1 is a very promising candidate as a photoactivated CO releasing moiety (photoCORM) with pote

36 e data could be useful for future studies of photoactivated Co-bleomycin and Co-bleomycin analogues i

37 driving forces in a suspension of synthetic photoactivated colloidal particles.

38 dicating that the contact is enhanced in the photoactivated complex.

39 that a Gln substitution at Glu-134 induces a photoactivated conformation around helices C and G even

40 PIF1 interacts specifically with the photoactivated conformer of phytochromes A and B, sugges

41 The photoactivated conformer of the photoreceptor Pfr has be

42 njugation of a CO-releasing molecule (here a photoactivated CORM) to the 5'-OH ribose group of vitami

43 ted by reacting mono-cysteine mutants with a photoactivated cross-linker.

44 biotin on the carboxy terminus, as well as a photoactivated cross-linking group (benzophenone), also

45 o the heteroduplex DNA, we have utilized the photoactivated cross-linking moiety 5-iododeoxyuridine (

46 -intercalating ruthenium complex to create a photoactivated cross-linking reagent.

47 Photoactivated cross-linking using the cysteine reagent

48 Here we show that both photoactivated cryptochromes repress COP1 activity throu

49 As such they approach ideality for photoactivated depot materials.

50 We previously described the photoactivated depot or PAD approach that allows for the

51 In this work, we create materials that form photoactivated depots of insulin without the need for po

52 rystal structures of both ground state and a photoactivated deprotonated intermediate of bovine rhodo

53 l transition from the crystal structure of a photoactivated deprotonated state of rhodopsin to the lo

54 Moreover, a photoactivated derivative of 25-HC does not cross-link t

55 act cells was demonstrated by showing that a photoactivated derivative of cholesterol cross-links to

56 the C-terminal transmembrane domain using a photoactivated derivative of cholesterol.

57 se metallointercalator-peptide conjugates by photoactivated DNA cleavage assays with the rhodium inte

58 y steady-state fluorescence measurements and photoactivated DNA cleavage experiments, reproduce the b

59 The first photoactivated doped quantum dot vector for metal-ion re

60 oresponsive materials, molecular motors, and photoactivated drugs.

61 lectivity of the probe and revealed that the photoactivated dye does not diffuse away from the origin

62 lso been shown that Verteporfin can have non-photoactivated effects such as interference with the YAP

63 nanoscale silver oxide (Ag2O) reveals strong photoactivated emission for excitation wavelengths short

64 sibly switch between stable dark-adapted and photoactivated end states.

65 mes onto dynein by combining live imaging of photoactivated endosomes and fluorescent dynein with mat

66 lity of proteins as targets of thermally and photoactivated enediynes has been confirmed at the molec

67 e is the first phytochrome with a non-kinase photoactivated enzymatic activity.

68 The photoactivated evolution of a series of enantiomerically

69 mer in the red light-absorbing state and the photoactivated far-red light-absorbing state revealed a

70 alogous experimental studies for the case of photoactivated Fe precatalyst 2 suggested that the activ

71 single-molecule fluorescence microscopy and photoactivated fluorescence microscopy.

72 ntitative measurements of the dissipation of photoactivated fluorescence show that, compared with MTs

73 l microscopy of fluorescent microspheres and photoactivated fluorescent dyes were used with well-diff

74 Both parallel MTs and photoactivated fluorescent marks on perpendicular MTs mo

75 The obtained results suggest that the photoactivated form of OCP represents a molten globule-l

76 tion factor, interacts specifically with the photoactivated form of phytochrome B (phyB).

77 Thus, photoactivated gene expression is a versatile tool for s

78 sophila embryos at gastrulation, we used the photoactivated gene expression system to mark small numb

79 ain progenitors, we created a fate map using photoactivated gene expression to mark cells originating

80 Photoactivated gene expression was also used either to k

81 By measuring changes in fluorescence of a photoactivated GFP protein targeted to mitochondria, we

82 eveloped an imaging method that combines the photoactivated green fluorescent protein (PA-GFP) and fl

83 re-activity relations, a ligand in which the photoactivated group was extended from the 3-position of

84 e mutant, designated BlgC, was found to have photoactivated guanylyl cyclase in vitro.

85 exchange and with nearly all of the reported photoactivated H/D exchange studies, the UV-induced H/D

86 UVA-photoactivated hypericin (0.5-2.0 J per cm2) could also

87 poptosis is, in part, one mechanism by which photoactivated hypericin inhibits malignant T cell proli

88 a caged form of thymosin beta4 (Tbeta4) was photoactivated in a defined local region of locomoting f

89 PAiRFPs can be photoactivated in a spatially selective manner in mouse

90 ChR2-transduced C1 neurons were photoactivated in conscious rats while EEG, neck muscle

91 alpha(2)-[P(2)W(17)O(61)](10-), that can be photoactivated in the presence of 2-propanol to transfer

92 ch to create therapeutic vectors that can be photoactivated in vivo with high spatial and temporal co

93 gh single-channel conductance but reversibly photoactivates in blue light.

94 cross-link supports the assignment that the photoactivated intermediate is the ketenimine or a keten

95 cross-strand cross-link, indicating that the photoactivated intermediate reacts broadly with function

96 e intermediates were very long-lived (>12 h, photoactivated lapachenole), others existed for millisec

97 Here, we combine photoactivated light microscopy with quantitative statis

98 ery of the photosensitizers, as well as, the photoactivating light and to establish dosimetric correl

99 sensitizer drugs are minimally toxic and the photoactivating light itself is non-ionizing.

100 sis of various stereopolymers by combining a photoactivated living polymerization, named photoinduced

101 d emission depletion (g-STED) microscopy and photoactivated localisation microscopy (PALM).

102 Here, we describe an approach to cryogenic photoactivated localization microscopy (cPALM) that perm

103 Here, we introduce interferometric photoactivated localization microscopy (iPALM), the comb

104 h two- and three-dimensional applications of photoactivated localization microscopy (PALM and iPALM,

105 By using photoactivated localization microscopy (PALM) and an exp

106 ase p56lck (Lck) using single and dual-color photoactivated localization microscopy (PALM) and direct

107 uperresolution microscopy techniques such as Photoactivated Localization Microscopy (PALM) and Stocha

108 se localization of single molecules, such as photoactivated localization microscopy (PALM) and stocha

109 per-resolution microscopy techniques such as photoactivated localization microscopy (PALM) and stocha

110 Here we expand the scope of SRM to photoactivated localization microscopy (PALM) imaging of

111 We used photoactivated localization microscopy (PALM) in rat hip

112 of molecular positions mapped via live-cell Photoactivated Localization Microscopy (PALM) is a power

113 Photoactivated localization microscopy (PALM) is a power

114 Photoactivated localization microscopy (PALM) is a power

115 l feature of most of the techniques based on photoactivated localization microscopy (PALM) or stochas

116 localization microscopy (SMLM)-for example, photoactivated localization microscopy (PALM) or stochas

117 Photoactivated localization microscopy (PALM) reveals th

118 lecule-based super-resolution imaging method Photoactivated Localization Microscopy (PALM) reveals th

119 We created a high-throughput modality of photoactivated localization microscopy (PALM) that enabl

120 We used quantitative photoactivated localization microscopy (PALM) to analyze

121 Here, we report the use of two-color photoactivated localization microscopy (PALM) to determi

122 We combined photoactivated localization microscopy (PALM) with live-

123 photoactivation microscopy, super-resolution photoactivated localization microscopy (PALM), and singl

124 In the present work, we demonstrate that photoactivated localization microscopy (PALM), in combin

125 ization of individual fluorescent molecules [photoactivated localization microscopy (PALM), stochasti

126 modeling, and single-molecule counting with photoactivated localization microscopy (PALM).

127 lution microscopy in whole fixed cells using photoactivated localization microscopy (PALM).

128 ated emission depletion (STED) microscopy or photoactivated localization microscopy (PALM).

129 lution techniques such as one- and two-color photoactivated localization microscopy (PALM).

130 ate live-cell super-resolution imaging using photoactivated localization microscopy (PALM).

131 rons at approximately 25 nm resolution using photoactivated localization microscopy (PALM).

132 The approach, called pair correlation photoactivated localization microscopy (PC-PALM), uses a

133 s using single particle tracking combined to photoactivated localization microscopy (sptPALM).

134 stochastic optical reconstruction microscopy/photoactivated localization microscopy (STORM/PALM) to d

135 Here, by combining super-resolution imaging (photoactivated localization microscopy [PALM]) and singl

136 Here we present a multicolour, photoactivated localization microscopy approach for imag

137 distribution of LacI in live bacteria using photoactivated localization microscopy combined with sin

138 ion of scaffold proteins was determined from photoactivated localization microscopy experiments, and

139 bimolecular fluorescence complementation and photoactivated localization microscopy for super-resolut

140 paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging method to

141 stochastic optical reconstruction microscopy/photoactivated localization microscopy imaging, and fluo

142 pecific protein degradation and quantitative photoactivated localization microscopy in strains with a

143 ngle-molecule localization microscopy (e.g., photoactivated localization microscopy or stochastic opt

144 mes less than mEos2, making Dendra2 a better photoactivated localization microscopy tag than mEos2 fo

145 Furthermore, we use photoactivated localization microscopy to determine the

146 Here, we use photoactivated localization microscopy to localize indiv

147 lso provides an example of applying tracking photoactivated localization microscopy to studies of low

148 We used single and two-color photoactivated localization microscopy to study complexe

149 ation microscopy (iPALM), the combination of photoactivated localization microscopy with single-photo

150 ion fluorescence microscopy (interferometric photoactivated localization microscopy) to map nanoscale

151 Here we describe a correlated cryo-PALM (photoactivated localization microscopy)-CET method for l

152 athway, in T cell membranes using high-speed photoactivated localization microscopy, dual-color fluor

153 sion depletion, structured illumination, and photoactivated localization microscopy, have enabled imp

154 Using fluorescence photoactivated localization microscopy, we detected full

155 Using photoactivated localization microscopy, we measured move

156 Using photoactivated localization microscopy, we reveal that t

157 We used this method--termed photoactivated localization microscopy--to image specifi

158 within a diffraction-limited area when using photoactivated localization microscopy.

159 timulated emission depletion microscopy, and photoactivated localization microscopy.

160 photoactivation localization microscopy and photoactivated localization microscopy.

161 opy, structured illumination microscopy, and photoactivated localization microscopy.

162 fluorescent protein and a popular marker for photoactivated localization microscopy.

163 ver, requires markers that can be reversibly photoactivated many times.

164 stribution of single BfpB molecules fused to photoactivated mCherry.

165 Cultured fibroblasts were exposed to photoactivated methylene blue under conditions that woul

166 Photoactivated methylene blue was used to damage purifie

167 Herein, we show that to preserve photoactivated molecular deformation on the macroscopic

168 One EPI-nitrene photoactivated molecule was incorporated in each subunit

169 g intracellular protein dynamics by tracking photoactivated molecules that are the only visible GFPs

170 ions of a central auditory optoprosthesis by photoactivating mouse auditory midbrain neurons that eit

171 This involves a photoactivated multicomponent reaction, performed both i

172 A photoactivated neutral organic super electron donor clea

173 The four photoactivated nitrene probes modified AChBP with up to

174 cation, engineering, and characterization of photoactivated nucleotidyl cyclases that can be used to

175 A single photoactivated OCP bound to the core of the PBS affords

176 te of photoactivity and the stability of the photoactivated OCP, suggesting that the activated OCP ha

177 e report a negative tone photoresist using a photoactivated olefin metathesis catalyst, which can be

178 For stimuli that photoactivated one rhodopsin per Galphat the rod OS swel

179 s require fluorophores whose emission can be photoactivated or photoswitched.

180 intermediates, and the less well understood photoactivated oxidative redding phenomenon.

181 The photoactivated PAmCherry1 exhibits a non-coplanar anioni

182 Photoactivated pattern formation in functional polymers

183 Here we demonstrate that photoactivated pattern formation on azobenzene-containin

184 ye-terminator sequencing fragments using the photoactivated PC chip and slab gel electrophoresis prod

185 nds several phototropins specifically in the photoactivated Pfr state.

186 ersion between a dark-adapted Pr state and a photoactivated Pfr state.

187 s evidence indicates that the binding of the photoactivated photoreceptor molecule to PIF3 induces ra

188 al mechanism of primary signal transfer from photoactivated photoreceptor to binding partner, and tha

189 ical and genetic evidence indicates that the photoactivated phy molecule acts within 60 s to induce t

190 lves direct, intranuclear interaction of the photoactivated phy molecule with PIF3, a basic helix-loo

191 adation induced by direct interaction of the photoactivated phy molecule with the transcription facto

192 llowing light-induced nuclear translocation, photoactivated phy molecules bind to and induce rapid ph

193 ivity is reversed upon exposure to light, by photoactivated phy molecules that induce degradation of

194 PPKs are directly involved in catalysing the photoactivated-phy-induced phosphorylation of PIF3 in vi

195 toactivated phyB nor artificial retention of photoactivated phyB in the cytosol provides detectable b

196 ng that the consequences of interaction with photoactivated phyB may differ among PIFs.

197 ther artificial nuclear translocation of non-photoactivated phyB nor artificial retention of photoact

198 hese data indicate that signal transfer from photoactivated phyB to its primary signaling partner(s)

199 gest the absence of a cytosolic pathway from photoactivated phyB to light-responsive genes.

200 ss, whereas in the light, the interaction of photoactivated phyB with PIF5 causes degradation of the

201 of the effects of PIF feedback modulation of photoactivated-phyB abundance.

202 scillates as a balance between synthesis and photoactivated-phytochrome-imposed degradation, with max

203 Moreover, photoactivated phytochromes bind to and induce rapid deg

204 vel phytochrome signaling mechanism in which photoactivated phytochromes directly interact with HMR a

205 icans grew on both media and produced a pink photoactivated pigment on m-DTDP agar.

206 In this manuscript, we discuss the use of photoactivated polycarbonate (PC) for purification of dy

207 We discuss the use of a photoactivated polycarbonate (PPC) microfluidic chip for

208 Perylenequinones are a class of photoactivated polyketide mycotoxins produced by fungal

209 orial stimulation by low-dose artemisinin to photoactivate PPIX to produce cytotoxic reactive oxygen.

210 Photoactivated probes have gained interest as experiment

211 Most bacteriophytochromes work as photoactivated protein kinases.

212 PALM/STORM rely on photoactivated proteins or photoswitching dyes, respecti

213 The method is applied to the photoactivated proton pump, bacteriorhodopsin, using con

214 We conclude that the photoactivated PYP domain in Ppr accelerates recovery of

215 In cultured mammalian cells, the effect of photoactivated pyr-TFO-directed DNA damage was to induce

216 Here we demonstrate that photoactivated pyrene-conjugated TFOs (pyr-TFOs) induce

217 be spectroscopy, and in elucidating the main photoactivated reactive paths.

218 The photoactivated receptor in DDM is dominated by one confo

219 nanodiscs, the ensemble of substates in the photoactivated receptor spontaneously decays to that cha

220 rmation of NpHtrII for signal relay from the photoactivated receptor to the kinase-binding region of

221 ins upon transition from the basic orange to photoactivated red OCP form.

222 In addition to transport, the photoactivated region frequently broadens, indicating th

223 A broadening of the photoactivated region occurred as MTs moved bidirectiona

224 Neurofilaments departed from the photoactivated regions at rapid velocities, but the over

225 Second, direct focal photoactivated release of the microtubule-stabilizing dr

226 Photoactivated reversible addition fragmentation chain t

227 Binding of purified Gt to photoactivated Rho (Rho*) in n-dodecyl beta-D-maltoside

228 onstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution

229 Photoactivated rhodopsin (R) catalyzes nucleotide exchan

230 robable protein-protein interactions between photoactivated rhodopsin (R*) and experimentally determi

231 The decay of photoactivated rhodopsin (R*) and inactivation of transd

232 reliable 3D model for the complex formed by photoactivated rhodopsin (R*) and its G-protein, transdu

233 nd utilized a model of the interface between photoactivated rhodopsin (R*) and transducin (Gt), its G

234 e of the G(t)alpha(340-350) peptide bound to photoactivated rhodopsin (R*) geometrically suggests a c

235 he interaction between transducin (G(t)) and photoactivated rhodopsin (R*), 14 analog peptides were d

236 ) as efficient in activating transduction as photoactivated rhodopsin (Rh*) in WT mice.

237 ated by phosphorylation of the C-terminus of photoactivated rhodopsin (Rh*).

238 Transitory binding between photoactivated rhodopsin (Rho* or Meta II) and the G pro

239 t subunit dissociation caused by exposure to photoactivated rhodopsin (Rho*).

240 the rhabdomere possibly by interacting with photoactivated rhodopsin 1 (Rh1*).

241 nal, is released in the rod outer segment by photoactivated rhodopsin after light excitation.

242 eased in rod photoreceptor outer segments by photoactivated rhodopsin and is eliminated through reduc

243 ouse rods, but the complex formation between photoactivated rhodopsin and transducin is severely comp

244 Complex formation between photoactivated rhodopsin and transducin was measured by

245 etinal rod cells involves phosphorylation of photoactivated rhodopsin by the enzyme rhodopsin kinase

246 nsducin, Gtalpha 340-350, within cavities of photoactivated rhodopsin formed by different energetical

247 er/Thr kinase responsible for termination of photoactivated rhodopsin in rod photoreceptor cells.

248 The ability of photoactivated rhodopsin to achieve the enzymatically ac

249 The release of all-trans retinal from photoactivated rhodopsin was measured in purified rod ou

250 ions is superstoichiometric to the amount of photoactivated rhodopsin, exceeding it by at least 30-fo

251 resulting spin-labeled Galphabetagamma with photoactivated rhodopsin, followed by rhodopsin-catalyze

252 GRK1 phosphorylates photoactivated rhodopsin, initiating steps in its deacti

253 inase") is necessary for the inactivation of photoactivated rhodopsin, the light receptor of the G-pr

254 dehyde all-trans-retinal that is released by photoactivated rhodopsin, to all-trans-retinol (vitamin

255 metarhodopsin II (MII), the conformation of photoactivated rhodopsin, which binds and activates tran

256 is visual arrestin responsible for quenching photoactivated rhodopsin, which moves into photoreceptor

257 state rhodopsin, and hydrolysis of SB using photoactivated rhodopsin.

258 ively compete with transducin for binding to photoactivated rhodopsin.

259 e retinal rod and cone photoreceptors, light photoactivates rhodopsin or cone visual pigments by conv

260 Corneal CXL with photoactivated riboflavin did not shorten the time to co

261 ter internalization, the photosensitizer was photoactivated, significantly increasing the cytotoxicit

262 We used photoactivated single-molecule tracking to discriminate

263 nd state (Pr) and a far-red-light-absorbing, photoactivated state (Pfr).

264 nerate a three-dimensional (3D) model of the photoactivated state (R*) of bovine rhodopsin (Rh).

265 ound state Pr, and a far-red light-absorbing photoactivated state Pfr.

266 bing dark state Pb and green light-absorbing photoactivated state Pg, combined with paired crystallog

267 In the photoactivated state, the Schiff base linking the chromo

268 enesis produced an enzyme with a more stable photoactivated state.

269 distance distributions in both the dark and photoactivated states of rhodopsin.

270 distance distributions in both the dark and photoactivated states of rhodopsin.

271 ts directly demonstrate the coupling of SRII photoactivated states to histidine kinase activity, prev

272 rotonated states that represent the dark and photoactivated states, respectively.

273 (PA-GFP) have been solved in the native and photoactivated states, which under 488 nm illumination a

274 A photoactivated-strengthening polymer network is reported

275 y between the active opsin structure and the photoactivated structures in crystallo near the retinal

276 orescence microscopy, and PAGFP was sparsely photoactivated such that polarized fluorescence identifi

277 bunit and used as sites of attachment of the photoactivated sulfhydryl reagent 4-(N-maleimido)benzoph

278 The gDNA was selectively captured on this photoactivated surface in an immobilization buffer, whic

279 ur results reveal that LH2 complexes undergo photoactivated switching to a quenched state, likely by

280 ormal actin polymerization kinetics, whereas photoactivated Tbeta4 significantly inhibited actin poly

281 These simulations demonstrated that locally photoactivating Tbeta4 in keratocytes could potentially

282 ricin used and the dose of light required to photoactivate the compound.

283 In search of strategies to photoactivate the luminescence of semiconductor quantum

284 e usefulness of this approach to selectively photoactivate the production of singlet oxygen in ROS st

285 block blood flow in the targeted artery, we photoactivated the rose bengal by illuminating the longi

286 We demonstrated its utility by photoactivating the beta-catenin pathway, achieving a tr

287 n of light can be used for a wide variety of photoactivated therapies, especially light-triggered DDS

288 esented as a compound II species that can be photoactivated to compound I.

289 roduct (e.g., chlorate and chlorite) through photoactivated transformations.

290 n is caused by an instability created by the photoactivated transitions between two immiscible states

291 les fail to migrate in a mutant deficient in photoactivated TRP calcium channels.

292 eutic devices with potential applications in photoactivated tumor therapy.

293 ected with the caged FAK peptide and locally photoactivated using a focused laser beam.

294 Photoactivated VCOP(D108A) had kinetics comparable to th

295 and likely reflects the spontaneous decay of photoactivated visual pigment.

296 The photoactivated W(CO)(6)/DABCO/THF system has been used f

297 Hypericin, photoactivated with 1.1-3.3 J white light per cm2, inhib

298 sh-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity viole

299 Hypericin, photoactivated with either UVA or white light, could ind

300 cs with theranostic nanoparticles, which are photoactivated within highly localized space near tumour