ライフサイエンスコーパス: photoaffinity

1 Detailed mapping of residues modified by the photoaffinity adducts may provide insight to guide the f

2 Using a photoaffinity agent, the molecular target has been ident

3 These have been characterized using the photoaffinity analog 8-azido-[alpha-32P]ATP as well as u

4 a molecular target for dantrolene using the photoaffinity analog [(3)H]azidodantrolene.

5 Experiments using a photoaffinity analog indicated that PSP binds to a singl

6 By using a photoaffinity analog of a potent respiratory syncytial v

7 st time, that [125I]iodoarylazidoprazosin, a photoaffinity analog of the substrate prazosin, labels m

8 5 nM, and an (125)I-labeled azido-Cys-AtPep1 photoaffinity analog specifically labeled a membrane-ass

9 Tritiated photoaffinity analogs of the natural lepidopteran juveni

10 was also specifically photolabeled by these photoaffinity analogs.

11 different numbers of cGMP moieties using the photoaffinity analogue 8-p-azidophenacylthio-cGMP; the r

12 We synthesized its photoaffinity analogue [(3)H](trifluoromethyl)phenyldiaz

13 We describe here the synthesis of the first photoaffinity analogue of SPC.

14 w here for the first time that both of these photoaffinity analogues are transport substrates for ABC

15 ]azidopine as well as the transport of these photoaffinity analogues by ABCG2.

16 Photoaffinity analogues of FPP (5, 6) were prepared by s

17 In this study, we show that photoaffinity analogues of HMBPP, meta/para-benzophenone

18 Two radiolabeled photoaffinity analogues of HTI-286, designated 4-benzoyl

19 The photoaffinity analogues were tested in a tubulin assembl

20 s for the production of analogues, including photoaffinity and biotinylated derivatives.

21 We show here, using photoaffinity and fluorescent derivatives, that this inh

22 Photoaffinity and radiolabeled analogues of MPC-6827 wer

23 ange of substituted aryl(CF(3))diazirines in photoaffinity applications.

24 These data validated the photoaffinity assay as an excellent system for the ident

25 assinosteroids to BRI1 using a biotin-tagged photoaffinity castasterone (BPCS), a biosynthetic precur

26 binant PfMDR1 protein with a highly specific photoaffinity CQ analogue, and lack of competition for p

27 ntical levels of PS1 derivatives that can be photoaffinity cross-linked to a biotinylated, benzopheno

28 group 9 A from the uridine base, we used the photoaffinity cross-linker 5N(3)dUTP with an azido group

29 th a fluorescent probe Alexa Fluor 680 and a photoaffinity cross-linker APG) was cross-linked to RyR1

30 Two approaches were used--photoaffinity cross-linking and disulfide chemical cross

31 In this study, we employed a photoaffinity cross-linking approach to map the CR4/5-TR

32 cted based on experimental data derived from photoaffinity cross-linking by psoralen, phenphi (cis-Rh

33 Site-directed photoaffinity cross-linking experiments were performed b

34 minal halves of the MukB coiled coil through photoaffinity cross-linking experiments.

35 Phenanthroline-copper footprinting and photoaffinity cross-linking indicate that TFIID makes ex

36 Photoaffinity cross-linking of the template to the modif

37 tion of the SH groups of these residues with photoaffinity cross-linking reagents caused a significan

38 bserved activity as well as mutagenicity and photoaffinity cross-linking studies of the alpha(v)beta(

39 Photoaffinity cross-linking studies provided evidence fo

40 Importantly, previous photoaffinity cross-linking studies using a PTH analogue

41 Combining our results from photoaffinity cross-linking studies with data now availa

42 We demonstrate here the use of photoaffinity cross-linking to determine high-resolution

43 bination of site-directed mutagenesis and UV photoaffinity cross-linking, we have identified five ami

44 ished findings from receptor mutagenesis and photoaffinity cross-linking.

45 eavily on benzoylphenylalanine- (Bpa-) based photoaffinity cross-linking.

46 y disulfide trapping extends and complements photoaffinity cross-linking.

47 ts into integrin-ligand interactions through photoaffinity, cross-linking/mass spectroscopy, and X-ra

48 Previous photoaffinity crosslinking data are used to position the

49 the connector in the procapsid was found by photoaffinity crosslinking.

50 Here, we have designed and synthesized photoaffinity derivatives of the 4-acyl-1,6-dialkylpiper

51 R activation also promotes the binding of a photoaffinity GTP analog to a protein that migrates on o

52 The cocaine photoaffinity label 3-iodo-4-azidococaine ([125I]IACoc)

53 ine analogs, we developed a piperidine-based photoaffinity label [(125)I]4-[2-(diphenylmethoxy)ethyl]

54 the previously identified attachment of the photoaffinity label [(125)I]RTI 82 in TM6.

55 s well as receptor-mediated incorporation of photoaffinity label [(32)P]azidoanilido-GTP indicates hi

56 We previously prepared a benztropine-based photoaffinity label [125I]-(N-[4-(4'-azido-3'-iodophenyl

57 [125I]TBZ-AIPP were synthesized and used to photoaffinity label chromaffin granule membranes.

58 his new analogue was explored by using it to photoaffinity label crude protein extracts obtained from

59 Using a synthetic signal peptide harboring a photoaffinity label in its hydrophobic core, we examined

60 hione and electrophilic substrate, acts as a photoaffinity label of dimeric rat liver glutathione S-t

61 ular reactivity is a desirable quality for a photoaffinity label to possess, and thus, the resistance

62 Bpa is a photoaffinity label unnatural amino acid that can form c

63 rier-free, radioiodinated fenpropimorph-like photoaffinity label, 1-N-(2',6'-dimethyl-morpholino)-3-(

64 Herein, we report a new photoaffinity label, 2-aryl-5-carboxytetrazole (ACT), th

65 e spanning region labeled by a cocaine-based photoaffinity label, [125I] 2 (RTI 82).

66 os-4-yloxy l)-2-propylamine ([(3)H]ATB-BMPA) photoaffinity label.

67 Mass spectrometric analysis of photoaffinity labeled bNT-CRFR1 yielded a 1:1 complex wi

68 closed state, we identified the amino acids photoaffinity labeled by [(125)I]TID in the presence of

69 Three photoaffinity labeled derivatives of epothilone D were p

70 in and phosphoinositide binding protein, was photoaffinity labeled using a variety of benzophenone-co

71 scoveries have emboldened efforts to prepare photoaffinity-labeled and other unique forms of STX as p

72 Using a photoaffinity-labeled ascr#2 probe and amplified lumines

73 In the present study, photoaffinity-labeled discodermolide analogues are used

74 Three photoaffinity-labeled discodermolide analogues were synt

75 te phosphopeptide fragments corresponding to photoaffinity-labeled fragments that contain all interna

76 The identification of photoaffinity-labeled peptides was aided by a signature

77 compounds reported in this study selectively photoaffinity-labeled the CCK receptor, resulting in the

78 LY294002, reduced the ability of TGase to be photoaffinity-labeled with [alpha-(32)P]GTP, providing e

79 A binding ensemble profiling with (f)photoaffinity labeling (BEProFL) approach that utilizes

80 The photochemistry of a new photoaffinity labeling (PAL) agent, 5-azido-2-(N,N-dieth

81 Photoaffinity labeling (PAL) was used to identify the bi

82 icate that this azide might be a very useful photoaffinity labeling agent, because the reactive inter

83 emonstrate the utility of these compounds as photoaffinity labeling analogues for the study of a vari

84 In this study, we used photoaffinity labeling and a proteomic approach to ident

85 Photoaffinity labeling and binding assays using transgen

86 In aggregate, results from the photoaffinity labeling and efflux assays using [(125)I]I

87 Although photoaffinity labeling and electron crystallographic stu

88 ral location of the site has been defined by photoaffinity labeling and electron crystallography, the

89 Photoaffinity labeling and fluorescence quenching experi

90 integrase (IN) inhibitor-binding site using photoaffinity labeling and mass spectrometric analysis.

91 sites of hTMPK inhibitors were validated by photoaffinity labeling and mass spectrometric studies.

92 eins were identified as filamin and actin by photoaffinity labeling and mass spectrometry.

93 This work focuses on the use of photoaffinity labeling and molecular modeling to elucida

94 in was confirmed by two separate approaches: photoaffinity labeling and site-specific antibodies.

95 The photoaffinity labeling and surface plasmon resonance-bas

96 es are also occupied, in a site suggested by photoaffinity labeling and thought to positively modulat

97 diazirines have achieved great popularity in photoaffinity labeling applications, the properties of t

98 t solution for all extant data, including 10 photoaffinity labeling constraints, a new such constrain

99 Combining the fluorescence data with photoaffinity labeling data provides insights into the c

100 tent with previous mutagenesis, chimera, and photoaffinity labeling data, demonstrating involvement o

101 When combined with previous photoaffinity labeling data, there are now seven indepen

102 based on experimental data from a series of photoaffinity labeling experiments and spectroscopic str

103 Although electron crystallography and photoaffinity labeling experiments determined that the b

104 BD1 can enhance the trapping of ADP at NBD2, photoaffinity labeling experiments with [alpha-(32)P]8-N

105 Recently, photoaffinity labeling experiments with mouse cell extra

106 tential drug binding site mimics and used in photoaffinity labeling experiments.

107 DNA photoaffinity labeling found that the Dpb4 subunit conta

108 Photoaffinity labeling identified two binding sites for

109 Photoaffinity labeling in a crude membrane fraction, fol

110 The present photoaffinity labeling in a physiologically relevant con

111 Photoaffinity labeling is a powerful tool to identify pr

112 Photoaffinity labeling is a useful technique employed to

113 entify the NAADP binding site, we employed a photoaffinity labeling method using a radioactive photop

114 This EET photoaffinity labeling method with a high signal-to-nois

115 elated and because the initial report of the photoaffinity labeling of a domain of this receptor incl

116 sin (IAAP) and [(3)H]azidopine were used for photoaffinity labeling of ABCG2 in this study.

117 ata demonstrating the efficient and specific photoaffinity labeling of CYP3A4 by this naturally occur

118 Further, the photoaffinity labeling of E969 indicated pore block as a

119 This is in contrast to photoaffinity labeling of FSH beta by the peptide mimic

120 Photoaffinity labeling of gamma-aminobutyric acid type A

121 e after sliding was also demonstrated by DNA photoaffinity labeling of histone proteins at specific s

122 Ing3A, but not PMA, blocked photoaffinity labeling of human P-gp with [(125)I]iodoar

123 Photoaffinity labeling of human protein geranylgeranyltr

124 We performed photoaffinity labeling of intact cells expressing fluore

125 Photoaffinity labeling of myofilament proteins with meta

126 mbination of whole cell transport assays and photoaffinity labeling of Pdr5p with [(125)I]iodoarylazi

127 Photoaffinity labeling of porcine SR with [(3)H]azidodan

128 g site is enriched in synaptic vesicles, and photoaffinity labeling of purified synaptic vesicles con

129 Photoaffinity labeling of Rab4 with [gamma-(32)P]GTP-azi

130 Photoaffinity labeling of Rab5 with [gamma-(32)P]GTP-azi

131 Photoaffinity labeling of receptors by bound agonists ca

132 Photoaffinity labeling of recombinant proteins identifie

133 DNA photoaffinity labeling of RSC showed that the Rsc4 subun

134 nicillenol analogue was also synthesized for photoaffinity labeling of target proteins.

135 Photoaffinity labeling of the AChR by (125)I-dizocilpine

136 h-affinity binding sites on the AChR; and 3) photoaffinity labeling of the AChR using (125)I-dizocilp

137 Photoaffinity labeling of the I-domain followed by LC/MS

138 In contrast, photoaffinity labeling of the M174A mutant using radioio

139 Photoaffinity labeling of the M181A mutant using radioio

140 bits in a concentration-dependent manner the photoaffinity labeling of the multidrug transporter with

141 rmediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibrobl

142 6-azi-3-hydroxypregnan-20-one (6-AziP), as a photoaffinity labeling reagent to identify neuroactive s

143 studies using the 8-azidoadenosine family of photoaffinity labeling reagents.

144 efore consistent with the conclusions of the photoaffinity labeling results.

145 Photoaffinity labeling showed that E226G, nevertheless,

146 ce constraints were utilized along with nine photoaffinity labeling spatial approximation constraints

147 The use of a photoaffinity labeling strategy identified fumarate hydr

148 esent study, we used a unique chemoselective photoaffinity labeling strategy, the methionine proximit

149 proximation constraints coming from previous photoaffinity labeling studies and 12 distance restraint

150 y that appears consistent with findings from photoaffinity labeling studies and with site-directed mu

151 Photoaffinity labeling studies have demonstrated approxi

152 Previous photoaffinity labeling studies have placed the B site on

153 erol stereoisomers were further confirmed by photoaffinity labeling studies on G(s),G(i2), and G(i3)

154 Photoaffinity labeling studies show radiolabeling of sub

155 Finally, photoaffinity labeling studies showed an isoform-specifi

156 d cocaine analog recognition was verified in photoaffinity labeling studies using [(125)I]MFZ 2-24.

157 Photoaffinity labeling studies with the 3-azidobenzoic a

158 By photoaffinity labeling studies, we previously observed a

159 eptor has come from receptor mutagenesis and photoaffinity labeling studies, with both contributing t

160 oximation constraints identified in previous photoaffinity labeling studies.

161 er to select a suitable candidate for future photoaffinity labeling studies.

162 ng to closed and open state models of TRPA1, photoaffinity labeling suggested that the A-967079 cavit

163 The photochemistry of the widely used photoaffinity labeling system 4-amino-3-nitrophenyl azid

164 lize SA analogs in conjunction with either a photoaffinity labeling technique or surface plasmon reso

165 identifying the protein targets of MOMIPP by photoaffinity labeling techniques.

166 This is in contrast with previous photoaffinity labeling through positions 6, 18, 22, and

167 e receptor (nAChR), which have been shown by photoaffinity labeling to bind to a common site in the i

168 we have utilized the more direct approach of photoaffinity labeling to explore spatial approximations

169 In this work, we have used photoaffinity labeling to identify a critical spatial ap

170 ze novel photoreactive fusion inhibitors and photoaffinity labeling to obtain direct physical evidenc

171 Here we apply photoaffinity labeling using a propofol derivative, meta

172 By photoaffinity labeling using derivatives of apicularen a

173 Previous mutagenesis studies and photoaffinity labeling using ligand analogues suggested

174 m the dyad was shown by DNA footprinting and photoaffinity labeling using recombinant histone octamer

175 Photoaffinity labeling was also increased in the presenc

176 The photoaffinity labeling was light- and concentration-depe

177 Photoaffinity labeling was protected by a molar excess o

178 In addition, photoaffinity labeling was used to determine the spatial

179 binant CRALBP (rCRALBP) was characterized by photoaffinity labeling with 3-diazo-4-keto-11-cis-retina

180 tyrosine mutants within the binding site by photoaffinity labeling with 5-azido-6-chloropyridin-3-yl

181 TP binding to both peptides was confirmed by photoaffinity labeling with 8-azido-ATP that was increas

182 We have employed photoaffinity labeling with 8-azido-ATP, which supports

183 Tryptophan fluorescence quenching and direct photoaffinity labeling with [(14)C]halothane suggested a

184 In this study, direct photoaffinity labeling with [11,12-(3)H]RA was used to i

185 Photoaffinity labeling with a fluorescent nonpolar pepti

186 Direct photoaffinity labeling with a full agonist probe confirm

187 Following photoaffinity labeling with all-trans-[11,12-(3)H]retino

188 Photoaffinity labeling with an epothilone A photoprobe l

189 ed and the hormone subunits were probed with photoaffinity labeling with receptor peptides correspond

190 pha4beta2 nAChRs was directly examined using photoaffinity labeling with the hydrophobic probe 3-(tri

191 atter conclusion based on previous data from photoaffinity labeling).

192 a combination of site-directed mutagenesis, photoaffinity labeling, amide hydrogen exchange, and try

193 lationship data, including binding affinity, photoaffinity labeling, and acquired mutation in human c

194 catalytic interactions using enzyme kinetic, photoaffinity labeling, and vanadate inhibition studies.

195 Photoaffinity labeling, chimera analysis, and mutagenesi

196 Using the combination of photoaffinity labeling, enzymatic digestion, MALDI-TOF a

197 ersus the completely unfolded state, we used photoaffinity labeling, hydrogen exchange, fluorescence

198 otein was supported by identification, using photoaffinity labeling, of a binding site for etomidate

199 Photoaffinity labeling, receptor site-directed mutagenes

200 specifically its binding site, which include photoaffinity labeling, site directed mutagenesis, and h

201 ing rotary shadowing electron microscopy and photoaffinity labeling, we mapped the binding site of de

202 cking, isothermal titration calorimetry, and photoaffinity labeling.

203 nucleosomal DNA regions by site-directed DNA photoaffinity labeling.

204 P and inositol phosphate (IP) induction, and photoaffinity labeling.

205 leosomes were probed using site-specific DNA photoaffinity labeling.

206 of binding of motilin to its receptor using photoaffinity labeling.

207 nt quench of intrinsic STAS fluorescence and photoaffinity labeling.

208 ironment for use in structural studies using photoaffinity labeling.

209 unit interface consistent with azi-etomidate photoaffinity labeling.

210 ibitor, and we identify its binding sites by photoaffinity labeling.

211 of subtype selectivity was examined here by photoaffinity labeling.

212 The results indicate that these photoaffinity-labeling agents are binding at the same al

213 is makes diazirines potentially more general photoaffinity-labeling agents.

214 ino acid residues identified in two separate photoaffinity-labeling experiments, (3) structure-activi

215 , evidenced by mRNA and protein analyses and photoaffinity-labeling experiments.

216 Potential photoaffinity-labeling ligands of the PLG binding site w

217 Six different photoaffinity-labeling ligands were designed and synthes

218 4-azidobenzoyl and 4-azido-2-hydroxybenzoyl photoaffinity-labeling moieties were placed at opposite

219 For Torpedo nAChR, photoaffinity-labeling studies with the competitive anta

220 ent with all existing structure-activity and photoaffinity-labeling studies.

221 Based on our previous studies using photoaffinity-labeling techniques in characterizing the

222 Photoaffinity labelling by using an analog of GTP demons

223 Here we report photoaffinity labelling of PS1 (and PS2) by potent gamma

224 Photoaffinity labels are powerful tools for dissecting l

225 DATs labeled with [(125)I]AD-96-129 or other photoaffinity labels displayed distinctive sensitivities

226 ]IAPEGlyMER and [125I]TBZ-AIPP are effective photoaffinity labels for VMAT2.

227 toactivatable analogues bearing benzophenone photoaffinity labels have been prepared.

228 e have been synthesized and characterized as photoaffinity labels of the vesicle monoamine transporte

229 a-1 and sigma-2 receptors, we show that both photoaffinity labels specifically and covalently derivat

230 ally positioned carrier-free, radioiodinated photoaffinity labels specifically designed to probe the

231 This is due to a lack of photoaffinity labels that are minimally modified from th

232 established total synthesis strategy and the photoaffinity labels were attached to the C26 hydroxyl g

233 ct that further development of this class of photoaffinity labels will lead to a broad range of appli

234 Traditional photoaffinity labels work through nonspecific C-H/X-H bo

235 iazirine and benzophenone, two commonly used photoaffinity labels, in two case studies ACT showed hig

236 be investigated using phosphorylnitrenes as photoaffinity labels.

237 rearrangements increases their usefulness as photoaffinity labels.

238 s sites involved in the binding of other DAT photoaffinity labels.

239 imic of exoloop 3 specifically and saturably photoaffinity-labels FSH alpha but not FSH beta.

240 ta2 nAChR at a single high-affinity site and photoaffinity-labels only the alpha4 subunit, presumably

241 ), in terms of their relative affinity for a photoaffinity ligand (2-azido-3-[(125)I]iodo-7,8-dibromo

242 d that the hAhR has a lower affinity for the photoaffinity ligand compared with mAhR(b-1).

243 The novel photoaffinity ligand N-[4-(4-azido-3-(125)I-iodophenyl)-

244 e, lapachenole has been used as an effective photoaffinity ligand of human P450 3A4, and mass spectro

245 Three photoaffinity ligands (PALs) for the human serotonin tra

246 While photoaffinity ligands (PALs) have been widely used to pr

247 ropofol binding sites have been mapped using photoaffinity ligands and mutagenesis; however, their pr

248 Tropane-based photoaffinity ligands covalently bind to discrete points

249 ize further these different binding domains, photoaffinity ligands that had the 4'-azido-3'-iodopheny

250 Photoaffinity ligands that were cross-linked to the rece

251 ounds of the 1,5-benzodiazepine CCK receptor photoaffinity ligands were originally prepared in an eff

252 eversible reactions, to be used as effective photoaffinity ligands.

253 d by use of mechanism-based inactivators and photoaffinity ligands.

254 Eight photoaffinity-modified residues were identified in rCRAL

255 22 bp fragment, we synthesized a (32)P- and photoaffinity moiety-labeled 22 bp dsRNA fragment and us

256 Using a bifunctional photoaffinity nucleotide analogue and a non-membrane-per

257 owever, BTN3A1 did not preferentially bind a photoaffinity prenyl pyrophosphate.

258 Therefore, we developed (1) a GSH-based photoaffinity probe (GSTABP-G) to target the "G site", a

259 cifically labeled by an active site-directed photoaffinity probe (III-63) for PS, indicating that the

260 ailored WOBE437-derived diazirine-containing photoaffinity probe (RX-055) irreversibly blocked membra

261 An angiotensin analog, photoaffinity probe 125I-SBpa-Ang II, was used to specif

262 gation of biotin to the C4S dodecasaccharide photoaffinity probe afforded a strategy for the isolatio

263 onist azidoepibatidine (AzEPI) prepared as a photoaffinity probe and radioligand.

264 ive site of gamma-secretase using a multiple photoaffinity probe approach called "photophore walking.

265 Both fluorescein and a benzophenone photoaffinity probe are incorporated, with total labelin

266 N-terminal fragment dimers exist by using a photoaffinity probe based on a transition state analog g

267 Here, we demonstrate that the photoaffinity probe E2012-BPyne specifically labels the

268 s-retinoic acid ([(3)H]9-cis-RA) as a direct photoaffinity probe for the characterization of human re

269 We have synthesized a state-dependent photoaffinity probe for the nicotinic acetylcholine rece

270 dine has been synthesized and evaluated as a photoaffinity probe for the putative transporter protein

271 biological evaluation of a novel paclitaxel photoaffinity probe is described.

272 Here we use a clickable photoaffinity probe to identify cathepsin D (CatD) as a

273 (TDBzcholine) was synthesized and used as a photoaffinity probe to map the orientation of an aromati

274 in the ion channel and that [(3)H]dFBr is a photoaffinity probe with broad amino acid side chain rea

275 riments using 100-fold excess unlabeled 2-5A photoaffinity probe, pApAp(8-azidoA), and authentic 2-5A

276 The newly synthesized, biotinylated photoaffinity probe, SQBAzide, was first shown to specif

277 Here we report the use of a novel photoaffinity probe, WC-21, to identify the sigma-2 rece

278 cleotide phosphate ([(32)P-5N(3)]NAADP) as a photoaffinity probe.

279 Here, we report that photoaffinity probes based on potent helical peptide inh

280 n of KAT bisubstrate inhibitors to clickable photoaffinity probes enables the selective covalent labe

281 yl compounds 1 and 2 are therefore candidate photoaffinity probes for characterization of both mammal

282 ), were prepared in good yields as candidate photoaffinity probes for mammalian and insect nicotinic

283 ications for designing oligosaccharide-based photoaffinity probes for the identification of proteins

284 This hypothesis can be tested by using two photoaffinity probes that differ only in the N-unsubstit

285 We designed and applied dynorphin-inspired photoaffinity probes to reveal the protein targets of th

286 ng the peptidoglycan biosynthetic machinery, photoaffinity probes were installed in combination with

287 We developed clickable GSM photoaffinity probes with the goal of identifying the ta

288 ChBP by using optimized azidochloropyridinyl photoaffinity probes.

289 o active site lysines can be cross-linked to photoaffinity probes.

290 ability of these proteins to cross-link the photoaffinity probes.

291 explore this, we examined the binding of the photoaffinity reagent 8-azido-ATP[gamma] biotin to purif

292 A system of photoaffinity reagents for selective labeling of DNA pol

293 rly demonstrate that chromenes are effective photoaffinity reagents for the cytochrome P450 superfami

294 Based on these results, we prepared photoaffinity reagents for the identification of the par

295 ave utilized two types of arylazido-modified photoaffinity reagents that probe residues in the Uvr pr

296 We used photoaffinity scanning (PAS) to identify key ligand-rece

297 The photoaffinity spin-labeled non-nucleoside ATP analogue,

298 mma-(32)P]triphosphate gamma-azidoanilide, a photoaffinity substrate analogue whose binding to Vrg4-H

299 ility to displace the substrate analogue and photoaffinity tag [(125)I]iodoarylazidoprazosin.

300 n of Rab5 with DATFP-FPP establishes a novel photoaffinity technique for the characterization of pren