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1 atter conclusion based on previous data from photoaffinity labeling).
2 os-4-yloxy l)-2-propylamine ([(3)H]ATB-BMPA) photoaffinity label.
3 d, namely, the residues which react with the photoaffinity label.
4 photophore, was chemically synthesized as a photoaffinity label.
5 s sites involved in the binding of other DAT photoaffinity labels.
6 be investigated using phosphorylnitrenes as photoaffinity labels.
7 rearrangements increases their usefulness as photoaffinity labels.
8 nucleosomal DNA regions by site-directed DNA photoaffinity labeling.
9 P and inositol phosphate (IP) induction, and photoaffinity labeling.
10 leosomes were probed using site-specific DNA photoaffinity labeling.
11 of binding of motilin to its receptor using photoaffinity labeling.
12 no acids in the RA-binding site of CRABPs by photoaffinity labeling.
13 , showed that cyclosporin A competed for the photoaffinity labeling.
14 saturable, indicating the specificity of the photoaffinity labeling.
15 characterized by fluorometric titration and photoaffinity labeling.
16 nt quench of intrinsic STAS fluorescence and photoaffinity labeling.
17 ironment for use in structural studies using photoaffinity labeling.
18 unit interface consistent with azi-etomidate photoaffinity labeling.
19 ibitor, and we identify its binding sites by photoaffinity labeling.
20 of subtype selectivity was examined here by photoaffinity labeling.
21 cking, isothermal titration calorimetry, and photoaffinity labeling.
22 rier-free, radioiodinated fenpropimorph-like photoaffinity label, 1-N-(2',6'-dimethyl-morpholino)-3-(
23 ine analogs, we developed a piperidine-based photoaffinity label [(125)I]4-[2-(diphenylmethoxy)ethyl]
24 ite, we designed and synthesized the agonist photoaffinity label [(125)I]iodoazidosalmeterol ([125I]I
26 We previously prepared a benztropine-based photoaffinity label [125I]-(N-[4-(4'-azido-3'-iodophenyl
28 el as the beta 2AR labeled by the antagonist photoaffinity label [125I]iodoazidobenzylpindolol ([125I
32 s well as receptor-mediated incorporation of photoaffinity label [(32)P]azidoanilido-GTP indicates hi
36 icate that this azide might be a very useful photoaffinity labeling agent, because the reactive inter
42 a combination of site-directed mutagenesis, photoaffinity labeling, amide hydrogen exchange, and try
43 emonstrate the utility of these compounds as photoaffinity labeling analogues for the study of a vari
48 he enzyme was demonstrated using 8-azido-ATP photoaffinity labeling and binding of trinitrophenyl (TN
51 ral location of the site has been defined by photoaffinity labeling and electron crystallography, the
52 te of Taxol in beta-tubulin as determined by photoaffinity labeling and electron crystallography.
54 vine serum albumin (BSA) and myoglobin using photoaffinity labeling and hydrogen-tritium exchange (HX
55 integrase (IN) inhibitor-binding site using photoaffinity labeling and mass spectrometric analysis.
56 sites of hTMPK inhibitors were validated by photoaffinity labeling and mass spectrometric studies.
59 FC protein was detected in parental cells by photoaffinity labeling and on Western blots with RFC-spe
61 in was confirmed by two separate approaches: photoaffinity labeling and site-specific antibodies.
63 es are also occupied, in a site suggested by photoaffinity labeling and thought to positively modulat
64 scoveries have emboldened efforts to prepare photoaffinity-labeled and other unique forms of STX as p
65 lationship data, including binding affinity, photoaffinity labeling, and acquired mutation in human c
66 catalytic interactions using enzyme kinetic, photoaffinity labeling, and vanadate inhibition studies.
67 diazirines have achieved great popularity in photoaffinity labeling applications, the properties of t
73 with both thalidomide and the nonradioactive photoaffinity label at concentrations comparable to thos
74 transcriptase (HIV-1 RT) was investigated by photoaffinity labeling based on catalytic competence.
76 the transporter impaired the binding of the photoaffinity label [beta-32P]5-azido-UDP-GlcUA to UDP-g
79 closed state, we identified the amino acids photoaffinity labeled by [(125)I]TID in the presence of
80 mammalian receptor: C. elegans AHR-1 is not photoaffinity labeled by a dioxin analog, and it is not
84 inhibitors, and protons have been found with photoaffinity labeling, chimeras, and single-site mutati
86 e-tagged S3 in bacterial lysates followed by photoaffinity labeling confirmed its specific labeling.
87 t solution for all extant data, including 10 photoaffinity labeling constraints, a new such constrain
88 his new analogue was explored by using it to photoaffinity label crude protein extracts obtained from
90 tent with previous mutagenesis, chimera, and photoaffinity labeling data, demonstrating involvement o
96 DATs labeled with [(125)I]AD-96-129 or other photoaffinity labels displayed distinctive sensitivities
98 based on experimental data from a series of photoaffinity labeling experiments and spectroscopic str
101 BD1 can enhance the trapping of ADP at NBD2, photoaffinity labeling experiments with [alpha-(32)P]8-N
105 ino acid residues identified in two separate photoaffinity-labeling experiments, (3) structure-activi
107 yrrolidinediol [ADP-HPD] were synthesized as photoaffinity labels for poly(ADP-ribose) glycohydrolase
110 te phosphopeptide fragments corresponding to photoaffinity-labeled fragments that contain all interna
112 hospholipase C, while immunoprecipitation of photoaffinity-labeled G-proteins from membranes indicate
115 ersus the completely unfolded state, we used photoaffinity labeling, hydrogen exchange, fluorescence
117 Using a synthetic signal peptide harboring a photoaffinity label in its hydrophobic core, we examined
121 M(r) 68 kDa and 18 kDa were gibberellin (GA)-photoaffinity labelled in vitro in plasma membrane prepa
122 iazirine and benzophenone, two commonly used photoaffinity labels, in two case studies ACT showed hig
129 entify the NAADP binding site, we employed a photoaffinity labeling method using a radioactive photop
132 4-azidobenzoyl and 4-azido-2-hydroxybenzoyl photoaffinity-labeling moieties were placed at opposite
133 hione and electrophilic substrate, acts as a photoaffinity label of dimeric rat liver glutathione S-t
134 henone], may have general applicability as a photoaffinity label of other enzymes with glutathione bi
135 trate, was synthesized and shown to act as a photoaffinity label of rat liver glutathione S-transfera
136 estingly, excess unlabeled ATP could enhance photoaffinity labeling of 8-azido-[alpha-32P]ATP to Vps3
137 elated and because the initial report of the photoaffinity labeling of a domain of this receptor incl
142 ata demonstrating the efficient and specific photoaffinity labeling of CYP3A4 by this naturally occur
148 e after sliding was also demonstrated by DNA photoaffinity labeling of histone proteins at specific s
154 to the two species previously identified by photoaffinity labeling of live cells as the HA receptor.
160 mbination of whole cell transport assays and photoaffinity labeling of Pdr5p with [(125)I]iodoarylazi
161 apping MgADP at the catalytic site inhibited photoaffinity labeling of Pgp with substrate analogues,
165 g site is enriched in synaptic vesicles, and photoaffinity labeling of purified synaptic vesicles con
173 h-affinity binding sites on the AChR; and 3) photoaffinity labeling of the AChR using (125)I-dizocilp
175 enitrificans and T. thermophilus established photoaffinity labeling of the equivalent bacterial NQO6.
179 bits in a concentration-dependent manner the photoaffinity labeling of the multidrug transporter with
181 ng and enzyme inhibition studies showed that photoaffinity labeling of the specific high-affinity bin
183 a (Lathyrus odoratus L.), lb, showed reduced photoaffinity labelling of both polypeptides compared wi
185 e have been synthesized and characterized as photoaffinity labels of the vesicle monoamine transporte
186 otein was supported by identification, using photoaffinity labeling, of a binding site for etomidate
187 ta2 nAChR at a single high-affinity site and photoaffinity-labels only the alpha4 subunit, presumably
191 rmediates formed during BER, we used a novel photoaffinity labeling probe and mouse embryonic fibrobl
192 rget of the SAHA-like HPCs, we synthesized a photoaffinity labeling reagent structurally based on SAH
193 6-azi-3-hydroxypregnan-20-one (6-AziP), as a photoaffinity labeling reagent to identify neuroactive s
201 specifically its binding site, which include photoaffinity labeling, site directed mutagenesis, and h
202 ce constraints were utilized along with nine photoaffinity labeling spatial approximation constraints
204 a-1 and sigma-2 receptors, we show that both photoaffinity labels specifically and covalently derivat
205 ally positioned carrier-free, radioiodinated photoaffinity labels specifically designed to probe the
207 esent study, we used a unique chemoselective photoaffinity labeling strategy, the methionine proximit
208 proximation constraints coming from previous photoaffinity labeling studies and 12 distance restraint
209 y that appears consistent with findings from photoaffinity labeling studies and with site-directed mu
213 erol stereoisomers were further confirmed by photoaffinity labeling studies on G(s),G(i2), and G(i3)
216 sed substrate analog, intending to use it in photoaffinity labeling studies to probe the luciferase a
217 d cocaine analog recognition was verified in photoaffinity labeling studies using [(125)I]MFZ 2-24.
221 eptor has come from receptor mutagenesis and photoaffinity labeling studies, with both contributing t
226 ng to closed and open state models of TRPA1, photoaffinity labeling suggested that the A-967079 cavit
227 desorption/ionization mass spectrometry of a photoaffinity labeled synthetic polypeptide representing
229 lize SA analogs in conjunction with either a photoaffinity labeling technique or surface plasmon reso
234 5)I]IAmF represents a new class of beta(2)AR photoaffinity labels that can directly probe the catecho
235 We now take the more direct approach of photoaffinity labeling the active site of the cholecysto
237 compounds reported in this study selectively photoaffinity-labeled the CCK receptor, resulting in the
239 We show that the binding of thalidomide photoaffinity label to authentic human AGP is competed w
240 ular reactivity is a desirable quality for a photoaffinity label to possess, and thus, the resistance
241 e receptor (nAChR), which have been shown by photoaffinity labeling to bind to a common site in the i
243 we have utilized the more direct approach of photoaffinity labeling to explore spatial approximations
245 ze novel photoreactive fusion inhibitors and photoaffinity labeling to obtain direct physical evidenc
247 in and phosphoinositide binding protein, was photoaffinity labeled using a variety of benzophenone-co
249 ol III with DNA that are not detected by DNA photoaffinity labeling using an aryl azide, fluorinated
252 m the dyad was shown by DNA footprinting and photoaffinity labeling using recombinant histone octamer
257 the detection of protein-DNA contacts by DNA photoaffinity labeling, we attached four different photo
258 ing rotary shadowing electron microscopy and photoaffinity labeling, we mapped the binding site of de
261 the present study patch clamp techniques and photoaffinity labeling were used in DMS-114 cells (a tum
262 n (1)H nuclear magnetic resonance and direct photoaffinity labeling were used in this study to charac
263 established total synthesis strategy and the photoaffinity labels were attached to the C26 hydroxyl g
264 ct that further development of this class of photoaffinity labels will lead to a broad range of appli
265 -polyacrylamide gel electrophoresis that was photoaffinity labeled with 5-(125)I-[3-(p-azidosalicylam
267 ith those obtained for dopamine transporters photoaffinity labeled with a GBR 12935 analog, [125I]1-[
271 binant CRALBP (rCRALBP) was characterized by photoaffinity labeling with 3-diazo-4-keto-11-cis-retina
272 tyrosine mutants within the binding site by photoaffinity labeling with 5-azido-6-chloropyridin-3-yl
273 TP binding to both peptides was confirmed by photoaffinity labeling with 8-azido-ATP that was increas
275 ze determination on a glycerol gradient, and photoaffinity labeling with 8-azidoguanosine-5'-[alpha-(
276 Tryptophan fluorescence quenching and direct photoaffinity labeling with [(14)C]halothane suggested a
281 s identified as the putative beta-subunit by photoaffinity labeling with a 32P-labeled analog of farn
282 oupled cholecystokinin (CCK) receptor, using photoaffinity labeling with a CCK analogue probe incorpo
286 r by a combination of column chromatography, photoaffinity labeling with an analog of ATP, and native
290 ed and the hormone subunits were probed with photoaffinity labeling with receptor peptides correspond
292 te dehydrogenase (GDH) were identified using photoaffinity labeling with the benzophenone nucleotide
293 ansport, drug-stimulated ATP hydrolysis, and photoaffinity labeling with the drug analogue, [125I]iod
294 pha4beta2 nAChRs was directly examined using photoaffinity labeling with the hydrophobic probe 3-(tri
296 biquinone reductase from Escherichia coli is photoaffinity-labeled with 3-azido-2-methyl-5-methoxy-[3
297 LY294002, reduced the ability of TGase to be photoaffinity-labeled with [alpha-(32)P]GTP, providing e
298 hormone, human choriogonadotropin (hCG) was photoaffinity-labeled with a peptide mimic corresponding
299 oincided with a radioactive band obtained by photoaffinity-labeling with N4alpha-azidobenzoyl-125I-AN
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