ライフサイエンスコーパス: photobleach

1 y FRET and FRAP (fluorescence recovery after photobleach).

2 ation, and rapid fluorescence recovery after photobleaching.

3 by the quantum yield of fluorophores and by photobleaching.

4 acteria cells achieved by regular SIRM after photobleaching.

5 asurements using fluorescence recovery after photobleaching.

6 aneously measuring fluorescence lifetime and photobleaching.

7 this artifact was measured using single-step photobleaching.

8 cle fusion using fluorescence recovery after photobleaching.

9 s analyzed using fluorescence recovery after photobleaching.

10 intensity, the excess intensity just adds to photobleaching.

11 omise the localization precision in stepwise photobleaching.

12 as verified by quantized photoactivation and photobleaching.

13 ly quantified by fluorescence recovery after photobleaching.

14 ular exchange by fluorescence recovery after photobleaching.

15 creased time for fluorescence recovery after photobleaching.

16 ed with PPIX fluorescence and degree of PPIX photobleaching.

17 fter photobleaching and fluorescence loss in photobleaching.

18 s needs to be considered when correcting for photobleaching.

19 ements for finite filament length as well as photobleaching.

20 heir different fluorescence stability during photobleaching.

21 rature-dependent fluorescence recovery after photobleaching.

22 applied a combination of biochemical assays, photobleaching/activation approaches, and atomistic mole

23 itions within a nucleus, without significant photobleaching, allowing us to make reliable estimates o

24 ive optical hardware, superior resistance to photobleaching, amenability to quantitation, and facile

25 Fluorescence recovery after photobleaching analysis combined with microtubule destab

26 Moreover, fluorescence recovery after photobleaching analysis demonstrates that HsSAS-6 is imm

27 Quantitative fluorescence recovery after photobleaching analysis indicates that PRC1 proteins rap

28 Fluorescence recovery after photobleaching analysis revealed that patterned depolari

29 Interestingly, fluorescence recovery after photobleaching analysis reveals differential mobility of

30 trated in vitro, fluorescence recovery after photobleaching analysis suggests interactions in vivo ar

31 Here, we use single-molecule photobleaching analysis to measure the probability of Cl

32 Using fluorescence recovery after photobleaching analysis, we first show that secretory ve

33 Using fluorescence recovery after photobleaching analysis, we show that H1.3 has superfast

34 isotropy, single-molecule tracking, and step photobleaching analysis.

35 trans-ROL)) in the neural retina following a photobleach and 5-fold lower retinyl esters in the RPE.

36 In contrast, quantum dots do not photobleach and have much wider Stokes shifts, but a pau

37 longer fluorescence lifetime, resistance to photobleaching and 10-100 times higher molar extinction

38 nd the ERC using fluorescence recovery after photobleaching and a novel sterol efflux assay.

39 ability, as evidenced by suppression of both photobleaching and blinking behavior.

40 These probes are non-photobleaching and can be used alongside fluorophores wi

41 s as measured by fluorescence recovery after photobleaching and caused chromosome decondensation simi

42 Secondary outcome measures were PPIX photobleaching and clinical local skin reactions, suppor

43 Fluorescence recovery after photobleaching and dynamic light scattering data indicat

44 Based on fluorescence recovery after photobleaching and endocytosis assays, integrin recyclin

45 Here, we employed continuous photobleaching and fluorescence correlation and cross-co

46 al stress, using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

47 e energy transfer using donor recovery after photobleaching and fluorescence lifetime imaging microsc

48 when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching.

49 Fluorescence loss in photobleaching and fluorescence recovery after photoblea

50 The effect of scanning speed on photobleaching and fluorescence yield is more remarkable

51 terizations with fluorescence recovery after photobleaching and FRET corroborate the formation of mul

52 Photobleaching and genetic perturbations showed that the

53 racked in native terminals with simultaneous photobleaching and imaging (SPAIM) to show that DCVs und

54 ith nearly isotropic spatial resolution, low photobleaching and low photodamage.

55 ften used as an acceptor but YFP is prone to photobleaching and pH changes.

56 Fluorescence photobleaching and photoactivation experiments also reve

57 n be exploited to study molecules exhibiting photobleaching and photoactivation.

58 d understanding of the mechanisms underlying photobleaching and photoblinking of fluorescent dyes has

59 Using photobleaching and photoconversion experiments in glial

60 challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quali

61 nges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scatt

62 To overcome issues with photobleaching and poor distinction between confocal and

63 y, we demonstrate using fluorescence loss in photobleaching and quantitative co-localization with chr

64 sessed by slowed fluorescence recovery after photobleaching and resistance to salt.

65 the use of a combination of single-molecule photobleaching and substoichiometric fluorescent labelin

66 ity of the method and the demonstration that photobleaching and the photophysical properties of the d

67 rials are often used for their resistance to photobleaching and their complex viewing-direction-depen

68 tamate uncaging, fluorescence recovery after photobleaching and transgenic mice expressing labeled PS

69 : A fluorescent dextran inside TATS lumen is photobleached, and signal recovery by diffusion of unble

70 er microsurgery, fluorescence recovery after photobleaching, and fluorescence correlation spectroscop

71 ng of single fluorescent proteins, step-wise photobleaching, and multiparameter spectroscopy, allows

72 eling protocols, fluorescence recovery after photobleaching, and single particle tracking.

73 light source and avoids autofluorescence and photobleaching, and target molecules can be detected spe

74 ation was subsequently reduced by additional photobleaching, and the diffusion of individual SRB mole

75 In contrast, photobleaching anterograde transport vesicles entering a

76 ons by employing fluorescence recovery after photobleaching as an in vivo assay to measure the influe

77 single-molecule fluorescence recovery after photobleaching assay to independently observe filament n

78 obabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes,

79 FRAP (fluorescence recovery after photobleaching) assays were used to demonstrate that BNR

80 se PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional su

81 the first time, fluorescence recovery after photobleaching at variable radius experiments with bimol

82 ciation, we used fluorescence recovery after photobleaching beam-size analysis to study the membrane

83 ti-colour emission process, and blinking and photobleaching behaviours of single tetrapods can be con

84 hoton excitation (2PE) and poorly understood photobleaching characteristics have made their implement

85 le group, about twice the amount of PPIX was photobleached compared to topical cream application.

86 MG complexes emit 2-fold more photons before photobleaching compared to organic dyes such as Cy5 and

87 otobleaching and fluorescence recovery after photobleaching data demonstrate that Mig1 shuttles const

88 These procedures can be applied to photobleaching data for any protein complex with large n

89 with the dynactin disruptor mycalolide B or photobleaching DCVs entering a synaptic bouton by retrog

90 Fluorescence recovery after photobleaching demonstrated increased CD44 mobility by l

91 essed by calcein fluorescence recovery after photobleach during intracellular [Ca] recording.

92 tation times, thereby drastically decreasing photobleaching during repeated scanning.

93 hen fractional mislabeling occurs as well as photobleaching during the imaging process, and reveals i

94 enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an

95 No notable photobleaching effect or degradation could be observed d

96 Our approach is based on cytochrome photobleaching effects observed in the resonance Raman s

97 a combination of high intensity UV pulses to photobleach epicardial NADH.

98 Photobleaching event counting is a single-molecule fluor

99 uorophores, and the possibility that several photobleaching events occur almost simultaneously.

100 ddition to complications such as overlapping photobleaching events that may arise from fluorophore in

101 noise, and does not require the counting of photobleaching events.

102 ross-linking and fluorescence recovery after photobleach experiments, and it helps resolve the long d

103 e microscopy and fluorescence recovery after photobleaching experiments and found that mycomembrane f

104 mework for quantitatively analyzing stepwise photobleaching experiments and shed light on the localiz

105 n dynamics using fluorescence recovery after photobleaching experiments and single-molecule imaging.

106 Fluorescence recovery after photobleaching experiments confirmed that the GJIC remai

107 Fluorescence recovery after photobleaching experiments demonstrated that subunit exc

108 Moreover, fluorescence recovery after photobleaching experiments in the nucleoplasm show a dec

109 in reporters and fluorescence recovery after photobleaching experiments in zebrafish embryos identifi

110 Photobleaching experiments indicated that co-assembly of

111 Finally, photobleaching experiments indicated that PIP2 binding i

112 Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role o

113 Photobleaching experiments reveal that centrosome-bound

114 Photobleaching experiments revealed that during normal i

115 Single-molecule fluorescence photobleaching experiments revealed that PopD formed mos

116 gth control, but fluorescence recovery after photobleaching experiments rule out the initial bolus mo

117 combination with fluorescence-recovery-after-photobleaching experiments, revealed that nicotine, acti

118 complemented by fluorescence recovery after photobleaching experiments, which reveal an inverse corr

119 id dynamics with fluorescence recovery after photobleaching experiments.

120 ere monitored by fluorescence recovery after photobleaching experiments.

121 pool with kinetics similar to those seen in photobleaching experiments.

122 Fluorescence loss in photobleaching (FLIP) and network analysis experiments r

123 e (the largest TIRF depth) to preferentially photobleach fluorescence from the lower layers and allow

124 is combined with fluorescence recovery after photobleaching, fluorescence correlation spectroscopy an

125 We use fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, a

126 tion spectroscopy to quantify the diffusion, photobleaching, fluorescence intermittency, and photocon

127 agellar transport particles, or reduction of photobleaching for live microtubule imaging.

128 Moreover, fluorescence recovery after photobleaching (FRAP) analysis demonstrated that exposur

129 ealed ghosts and fluorescence recovery after photobleaching (FRAP) analysis of actin filament mobilit

130 determined using fluorescence recovery after photobleaching (FRAP) and binding, which is widely used

131 s we show, using fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy measur

132 ical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spect

133 Fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spect

134 -PEO) film using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking (SMT)

135 Using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking in hu

136 chia coli, using Fluorescence Recovery after Photobleaching (FRAP) and Total Internal Reflection Fluo

137 ation (FDAP) and fluorescence recovery after photobleaching (FRAP) are well established approaches fo

138 oscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determi

139 y was to develop fluorescence recovery after photobleaching (FRAP) as a technique to accurately and r

140 reased claudin 4 fluorescence recovery after photobleaching (FRAP) dynamics in response to inflammato

141 Fluorescence recovery after photobleaching (FRAP) experiments confirmed the differen

142 minutes, whereas fluorescence recovery after photobleaching (FRAP) experiments employing overexpressi

143 method based on fluorescence recovery after photobleaching (FRAP) for determining how many reaction

144 es by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-t

145 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental

146 Fluorescence recovery after photobleaching (FRAP) is an excellent tool to measure th

147 scent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching wit

148 Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the di

149 were analyzed by fluorescence recovery after photobleaching (FRAP) microscopy.

150 emonstrated using fluorescent recovery after photobleaching (FRAP) monitoring displacement of GFP-BAZ

151 as studied using fluorescence recovery after photobleaching (FRAP) of GFP-Galphas.

152 Fluorescence recovery after photobleaching (FRAP) of labeled protein demonstrated th

153 experiments and fluorescence recovery after photobleaching (FRAP) of SC junctions in utricles from m

154 Interestingly, fluorescence recovery after photobleaching (FRAP) results indicated that NKKY101 mut

155 ell imaging with fluorescence recovery after photobleaching (FRAP) revealed that the population of Ga

156 Notably, fluorescence recovery after photobleaching (FRAP) shows that YscQ exchanges between

157 Fluorescence recovery after photobleaching (FRAP) studies indicate that like H1, bin

158 ssed receptor by fluorescence recovery after photobleaching (FRAP) to demonstrate that endoglin forms

159 cribe the use of fluorescence recovery after photobleaching (FRAP) to probe chain mobility in reversi

160 scopy (FCCS) and fluorescence recovery after photobleaching (FRAP) we found that the integrin adhesom

161 We performed fluorescent recovery after photobleaching (FRAP), quantitative RT-PCR, and whole ce

162 Using fluorescence recovery after photobleaching (FRAP), we demonstrate that adherens junc

163 rc together with fluorescence recovery after photobleaching (FRAP), we find that Src significantly re

164 Performing acceptor-photobleaching FRET studies with receptors 1, 2, and 4,

165 Perturbation of equilibrium distributions by photobleaching has also been developed into a robust met

166 Single-molecule photobleaching has emerged as a powerful non-invasive ap

167 queous solutions of NAP SOA were observed to photobleach (i.e., lose their ability to absorb visible

168 ncluding inverse fluorescence recovery after photobleaching (iFRAP) and photoactivatable probes, coup

169 Fluorescence recovery after photobleaching imaging reveals that the S561A mutant sho

170 e complex can be revealed by single-molecule photobleaching imaging.

171 Photoconversion, photobleaching, immunofluorescence and super-resolution

172 n trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo.

173 OM), which are widely distributed but highly photobleached in the surface ocean, are critical in regu

174 uorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment.

175 scent signals in the presence of fluorophore photobleaching in a solid surface bioassay.

176 butable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with

177 been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful

178 We show using fluorescence recovery after photobleaching in hippocampal neurons that the majority

179 stress by using fluorescence recovery after photobleaching in proplatelets with fluorescence-tagged

180 s and studies of fluorescence recovery after photobleaching in respiratory mucus showed that mechanis

181 Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mic

182 Photobleaching is a major limitation of superresolution

183 Localization by stepwise photobleaching is especially suited for measuring nanome

184 theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluo

185 We confirm that STED Photobleaching is primarily caused by the depletion ligh

186 dard for experiments in which recovery after photobleaching is used to measure lateral diffusion.

187 labels (i.e., maximum emitted photons before photobleaching) is a critical requirement for achieving

188 ticles in a region of interest by repeatedly photobleaching its boundary.

189 rom quantitative analysis of single-molecule photobleaching kinetics without using SPT.

190 ameters were optimized to deliver 23.8 mJ of photobleaching light energy at a pulse width of 6 msec a

191 er they possess narrow Stokes shifts and can photobleach, limiting multiplexed detection applications

192 st, we visualized whole eisosomes and, after photobleaching, localized recruitment of new Pil1p molec

193 s in cancer phototherapy is often limited by photobleaching, low tumor selectivity, and tumor hypoxia

194 In addition, their strong resistance to photobleaching makes them suitable for long duration or

195 Fluorescence recovery after photobleaching measurements revealed that single mismatc

196 Fluorescence recovery after photobleaching measurements showed that both proteins co

197 her supported by fluorescence recovery after photobleaching measurements, which showed that, at heter

198 Our FRAP (fluorescence recovery after photobleaching) measurements showed that the complexes r

199 ne by using FRET microscopy and the acceptor photobleaching method.

200 cted by dye concentration, light scattering, photobleaching, micro-viscosity, temperature, or the mai

201 via multiphoton fluorescence recovery after photobleaching (MP-FRAP) of injected FITC-BSA, a 32.6% d

202 Thus, as fluorophores stochastically photobleach, noise properties of the time trace change s

203 vant information that may be acquired before photobleaching occurs.

204 ed probe DNA on these surfaces is unlabeled, photobleaching of a probe label is not an issue, allowin

205 luorescence and reduce photocarbonization or photobleaching of analytes.

206 In contrast to NAP SOA, the photobleaching of BrC material produced by the reaction

207 sensitive cells with no phototoxicity and no photobleaching of fluorescent biomarkers.

208 toactivation and fluorescence recovery after photobleaching of fluorescently tagged AMPAR to show tha

209 ess this question, we performed quantitative photobleaching of GFP-tagged AtCESA3-containing particle

210 ic receptor (M2R) and Gi1 by single-particle photobleaching of immobilized complexes.

211 ong-term detection is partially prevented by photobleaching of organic fluorescent probes.

212 lid phase micro extraction (SPME-GC-MS), and photobleaching of photosensitizers in milk (riboflavin,

213 Photobleaching of rhodopsin function prevents accumulati

214 ation, the fundamental molecular event after photobleaching of rhodopsin is the recombination reactio

215 hat PKM2 phosphorylation is signaled through photobleaching of rhodopsin.

216 s technique remain present such as the rapid photobleaching of several types of organic fluorophores

217 Stepwise photobleaching of SpoIVFB fused to a fluorescent protein

218 the vesicle lumen domain of Sb2, and perform photobleaching of YpH fluorophores.

219 d subsequently observing as the fluorophores photobleach, one obtains information on the number of su

220 rtant, as many dyes suffer from either rapid photobleaching or high nonspecific staining.

221 scopy as well as fluorescence recovery after photobleaching or photoswitching, and observed significa

222 s offer a superior optical signal and do not photobleach, our novel protocol holds enormous promise o

223 ted a reduced activation rate and an altered photobleaching pattern.

224 The photobleaching patterns of eGFP-M2R were indicative of a

225 n the quantification of photodarkening (PD), photobleaching (PB) and transient PD (TPD) in a-Ge(x)As(

226 tical properties to fluoresce with near-zero photobleaching, photoblinking and background autofluores

227 rastructure, and fluorescence recovery after photobleaching/photoconversion experiments showed that t

228 ted polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes.

229 , incomplete saturation of binding sites, or photobleaching produces stochastic mixtures.

230 aging, including fluorescence recovery after photobleaching, provided further support for the role of

231 Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation

232 hotophysical parameters of the probe such as photobleaching quantum yield, count rate per molecule, a

233 This includes photobleaching, quenching, and the formation of non-emis

234 In this paper, we show that the photobleaching rate in STED microscopy can be slowed dow

235 tive redox reactions that contributed to the photobleaching rate were studied over a wide temperature

236 namics, time-resolved spectroscopic methods (photobleach recovery, fluorescence correlation, single-m

237 ter simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-m

238 her increasing the linear scanning speed for photobleaching reduction in STED microscopy.

239 ins was assessed in FRAP studies of circular photobleached regions ( approximately 7 mum in diameter)

240 uce a method called smPReSS, single-molecule photobleaching relaxation to steady state, that infers e

241 Photobleaching remains a limiting factor in superresolut

242 ion coefficient, low quantum yield, and high photobleaching resistance.

243 TIR-fluorescence recovery after photobleaching revealed a significant recovery of tPA-ce

244 The use of fluorescence recovery after photobleaching revealed an increase in plasma membrane f

245 Fluorescence recovery after photobleaching revealed that the viscosity of CF ASL was

246 Fluorescence recovery after photobleaching reveals reversible changes in the level o

247 s include mechanical uncertainties, specimen photobleaching, segmentation, and stitching inaccuracies

248 xperiments using fluorescence recovery after photobleaching show that human FZD4 assembles-in a DVL-i

249 Stepwise photobleaching showed that CaMKII formed oligomeric comp

250 The analysis of fluorescence recovery after photobleaching showed that the fluxes of dye molecules i

251 Fluorescence recovery after photobleaching shows that TSSC1 is required for efficien

252 fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) a

253 gy to perform single-molecule recovery after photobleaching (SRAP) within dense macromolecular assemb

254 ty traces over time is the quantification of photobleaching step counts.

255 sian analysis of images collected during the photobleaching step of each plane enabled lateral superr

256 Although photobleaching steps are often detected by eye, this met

257 Our method is capable of detecting >/=50 photobleaching steps even for signal-to-noise ratios as

258 orithms, it is possible to reliably identify photobleaching steps for up to 20-30 fluorophores and si

259 ties have created a challenge in identifying photobleaching steps in a time trace.

260 factors can limit and bias the detection of photobleaching steps, including noise, high numbers of f

261 ta receptor with fluorescence recovery after photobleaching studies on the lateral diffusion of a coe

262 Fluorescence recovery after photobleaching studies showed that these RTNLBs are mobi

263 linked Orai1 concatemers and single-molecule photobleaching suggest that channels assemble as tetrame

264 A-wave recovery compared with WT mice after photobleaching, suggesting a delayed dark adaptation.

265 Using fluorescence recovery after photobleaching technique, diffusion coefficients D of fl

266 wo-photon absorption cross-section and rapid photobleaching tendency, their applications in two-photo

267 ters are frequently degraded by blinking and photobleaching that arise from poorly passivated host cr

268 CDOM origin (terrestrial versus marine) and photobleaching that controls variations in AQYs, with a

269 sion characteristics, including blinking and photobleaching that limit their utility and performance.

270 excitation zone by sequentially imaging and photobleaching the fluorescent molecules.

271 w blinking characteristics due to reversible photobleaching, the blinking of GNPs seems to be stable

272 After photobleaching, the EB1 signal at the flagellar tip reco

273 Irradiation with laser light above the photobleaching threshold induces photonic confinement po

274 The noise properties in a photobleaching time trace depend on the number of active

275 t a new Bayesian method of counting steps in photobleaching time traces that takes into account stoch

276 Here, we applied single-molecule photobleaching to analyze the oligomeric state of an end

277 a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial

278 GJs by applying fluorescence recovery after photobleaching to GJs formed from connexins fused with f

279 the anthropogenic source shows a shift from photobleaching to photohumification denoted by an increa

280 we use fluorophore localization imaging with photobleaching to probe the structure of EGFR oligomers.

281 including using chemical cleavage instead of photobleaching to remove fluorescent signals between con

282 st successful application of single-molecule photobleaching to resolve drug-induced and domain-depend

283 ment of photosynthetic efficiency and became photobleached under high light (HL) growth conditions.

284 at conventional fluorophores undergo minimal photobleaching under standard illumination in the FDS.

285 DA was assessed at baseline in 1 eye after a photobleach using a computerized dark adaptometer with t

286 The technique of Fluorescence Recovery After Photobleaching was applied for the first time on real ch

287 ured by 2-photon fluorescence recovery after photobleaching, was not affected just after cardiorespir

288 Using fluorescence recovery after photobleaching we show here that beta-arrestin is requir

289 By fluorescence recovery after photobleaching, we demonstrate a high mobility and fast

290 rgy transfer and fluorescence recovery after photobleaching, we demonstrate that arrestin-3 dissociat

291 plete recovery of dextran fluorescence after photobleaching, we demonstrated that the actin ring-asso

292 Through live-cell photobleaching, we find rapid binding kinetics between P

293 Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (

294 urthermore using fluorescence recovery after photobleaching, we found that FAK inhibition increased t

295 ited in observation time and photon count by photobleaching, we present a description of the sources

296 Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that caus

297 Using fluorescence recovery after photobleaching, we show that the ER chaperone Kar2/BiP f

298 lapse imaging of fluorescence recovery after photobleaching, we show that X11alpha is present in a mo

299 or wood smoke BrC, both photoenhancement and photobleaching were observed.

300 per molecule, the saturation intensity, the photobleaching yield, and, crucially, management of brig