ライフサイエンスコーパス: photobleaching

1 ation, and rapid fluorescence recovery after photobleaching.

2 by the quantum yield of fluorophores and by photobleaching.

3 acteria cells achieved by regular SIRM after photobleaching.

4 asurements using fluorescence recovery after photobleaching.

5 aneously measuring fluorescence lifetime and photobleaching.

6 this artifact was measured using single-step photobleaching.

7 cle fusion using fluorescence recovery after photobleaching.

8 s analyzed using fluorescence recovery after photobleaching.

9 intensity, the excess intensity just adds to photobleaching.

10 omise the localization precision in stepwise photobleaching.

11 as verified by quantized photoactivation and photobleaching.

12 ly quantified by fluorescence recovery after photobleaching.

13 ular exchange by fluorescence recovery after photobleaching.

14 creased time for fluorescence recovery after photobleaching.

15 ed with PPIX fluorescence and degree of PPIX photobleaching.

16 fter photobleaching and fluorescence loss in photobleaching.

17 ion over hundreds of volumes with negligible photobleaching.

18 urements such as fluorescence recovery after photobleaching.

19 s needs to be considered when correcting for photobleaching.

20 ements for finite filament length as well as photobleaching.

21 heir different fluorescence stability during photobleaching.

22 rature-dependent fluorescence recovery after photobleaching.

23 applied a combination of biochemical assays, photobleaching/activation approaches, and atomistic mole

24 itions within a nucleus, without significant photobleaching, allowing us to make reliable estimates o

25 atural diurnal conditions resulted in little photobleaching, although growth was slower.

26 ive optical hardware, superior resistance to photobleaching, amenability to quantitation, and facile

27 Fluorescence recovery after photobleaching analysis combined with microtubule destab

28 Moreover, fluorescence recovery after photobleaching analysis demonstrates that HsSAS-6 is imm

29 Quantitative fluorescence recovery after photobleaching analysis indicates that PRC1 proteins rap

30 Fluorescence recovery after photobleaching analysis of Sec8 at cell protrusions reve

31 Fluorescence recovery after photobleaching analysis revealed that patterned depolari

32 Interestingly, fluorescence recovery after photobleaching analysis reveals differential mobility of

33 trated in vitro, fluorescence recovery after photobleaching analysis suggests interactions in vivo ar

34 Here, we use single-molecule photobleaching analysis to measure the probability of Cl

35 munostaining and fluorescence recovery after photobleaching analysis, suggesting that Shootin1 is a n

36 Using fluorescence recovery after photobleaching analysis, we first show that secretory ve

37 Using fluorescence recovery after photobleaching analysis, we show that cortical LGN is dy

38 Using fluorescence recovery after photobleaching analysis, we show that H1.3 has superfast

39 isotropy, single-molecule tracking, and step photobleaching analysis.

40 longer fluorescence lifetime, resistance to photobleaching and 10-100 times higher molar extinction

41 nd the ERC using fluorescence recovery after photobleaching and a novel sterol efflux assay.

42 ability, as evidenced by suppression of both photobleaching and blinking behavior.

43 These probes are non-photobleaching and can be used alongside fluorophores wi

44 s as measured by fluorescence recovery after photobleaching and caused chromosome decondensation simi

45 Secondary outcome measures were PPIX photobleaching and clinical local skin reactions, suppor

46 Fluorescence recovery after photobleaching and dynamic light scattering data indicat

47 Based on fluorescence recovery after photobleaching and endocytosis assays, integrin recyclin

48 Here, we employed continuous photobleaching and fluorescence correlation and cross-co

49 al stress, using fluorescence recovery after photobleaching and fluorescence correlation spectroscopy

50 e energy transfer using donor recovery after photobleaching and fluorescence lifetime imaging microsc

51 when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching.

52 Fluorescence loss in photobleaching and fluorescence recovery after photoblea

53 The effect of scanning speed on photobleaching and fluorescence yield is more remarkable

54 terizations with fluorescence recovery after photobleaching and FRET corroborate the formation of mul

55 Photobleaching and genetic perturbations showed that the

56 racked in native terminals with simultaneous photobleaching and imaging (SPAIM) to show that DCVs und

57 ith nearly isotropic spatial resolution, low photobleaching and low photodamage.

58 ften used as an acceptor but YFP is prone to photobleaching and pH changes.

59 Fluorescence photobleaching and photoactivation experiments also reve

60 n be exploited to study molecules exhibiting photobleaching and photoactivation.

61 d understanding of the mechanisms underlying photobleaching and photoblinking of fluorescent dyes has

62 Using photobleaching and photoconversion experiments in glial

63 challenging for biological imaging as noise, photobleaching and phototoxicity compromise signal quali

64 nges in biological imaging include labeling, photobleaching and phototoxicity, as well as light scatt

65 To overcome issues with photobleaching and poor distinction between confocal and

66 y, we demonstrate using fluorescence loss in photobleaching and quantitative co-localization with chr

67 sessed by slowed fluorescence recovery after photobleaching and resistance to salt.

68 the use of a combination of single-molecule photobleaching and substoichiometric fluorescent labelin

69 ity of the method and the demonstration that photobleaching and the photophysical properties of the d

70 rials are often used for their resistance to photobleaching and their complex viewing-direction-depen

71 tamate uncaging, fluorescence recovery after photobleaching and transgenic mice expressing labeled PS

72 er microsurgery, fluorescence recovery after photobleaching, and fluorescence correlation spectroscop

73 functional analysis, it does not suffer from photobleaching, and it allows near real-time imaging in

74 ng of single fluorescent proteins, step-wise photobleaching, and multiparameter spectroscopy, allows

75 eling protocols, fluorescence recovery after photobleaching, and single particle tracking.

76 light source and avoids autofluorescence and photobleaching, and target molecules can be detected spe

77 ation was subsequently reduced by additional photobleaching, and the diffusion of individual SRB mole

78 In contrast, photobleaching anterograde transport vesicles entering a

79 ons by employing fluorescence recovery after photobleaching as an in vivo assay to measure the influe

80 single-molecule fluorescence recovery after photobleaching assay to independently observe filament n

81 obabilities, in conjunction with fluorophore photobleaching assays on over 2000 individual complexes,

82 l adhesions, and fluorescence recovery after photobleaching assays with GFP-Myo1c mutants revealed th

83 FRAP (fluorescence recovery after photobleaching) assays were used to demonstrate that BNR

84 se PolC functions in B. subtilis, we applied photobleaching-assisted microscopy, three-dimensional su

85 the first time, fluorescence recovery after photobleaching at variable radius experiments with bimol

86 ciation, we used fluorescence recovery after photobleaching beam-size analysis to study the membrane

87 ti-colour emission process, and blinking and photobleaching behaviours of single tetrapods can be con

88 ed EGFP-occludin fluorescence recovery after photobleaching, but TNF treatment did not affect behavio

89 hoton excitation (2PE) and poorly understood photobleaching characteristics have made their implement

90 MG complexes emit 2-fold more photons before photobleaching compared to organic dyes such as Cy5 and

91 otobleaching and fluorescence recovery after photobleaching data demonstrate that Mig1 shuttles const

92 These procedures can be applied to photobleaching data for any protein complex with large n

93 with the dynactin disruptor mycalolide B or photobleaching DCVs entering a synaptic bouton by retrog

94 Fluorescence recovery after photobleaching demonstrated increased CD44 mobility by l

95 tation times, thereby drastically decreasing photobleaching during repeated scanning.

96 hen fractional mislabeling occurs as well as photobleaching during the imaging process, and reveals i

97 enzyme-dependent fluorescence recovery after photobleaching (ED-FRAP) of NADH has been shown to be an

98 No notable photobleaching effect or degradation could be observed d

99 Our approach is based on cytochrome photobleaching effects observed in the resonance Raman s

100 Photobleaching event counting is a single-molecule fluor

101 uorophores, and the possibility that several photobleaching events occur almost simultaneously.

102 ddition to complications such as overlapping photobleaching events that may arise from fluorophore in

103 noise, and does not require the counting of photobleaching events.

104 e microscopy and fluorescence recovery after photobleaching experiments and found that mycomembrane f

105 mework for quantitatively analyzing stepwise photobleaching experiments and shed light on the localiz

106 n dynamics using fluorescence recovery after photobleaching experiments and single-molecule imaging.

107 Fluorescence recovery after photobleaching experiments confirmed that the GJIC remai

108 Fluorescence recovery after photobleaching experiments demonstrated that subunit exc

109 Moreover, fluorescence recovery after photobleaching experiments in the nucleoplasm show a dec

110 in reporters and fluorescence recovery after photobleaching experiments in zebrafish embryos identifi

111 Photobleaching experiments indicated that co-assembly of

112 Finally, photobleaching experiments indicated that PIP2 binding i

113 Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role o

114 Photobleaching experiments reveal that centrosome-bound

115 Photobleaching experiments revealed that during normal i

116 Single-molecule fluorescence photobleaching experiments revealed that PopD formed mos

117 gth control, but fluorescence recovery after photobleaching experiments rule out the initial bolus mo

118 Fluorescence loss in photobleaching experiments show subnuclear concentration

119 combination with fluorescence-recovery-after-photobleaching experiments, revealed that nicotine, acti

120 complemented by fluorescence recovery after photobleaching experiments, which reveal an inverse corr

121 id dynamics with fluorescence recovery after photobleaching experiments.

122 ere monitored by fluorescence recovery after photobleaching experiments.

123 pool with kinetics similar to those seen in photobleaching experiments.

124 Fluorescence loss in photobleaching (FLIP) and network analysis experiments r

125 is combined with fluorescence recovery after photobleaching, fluorescence correlation spectroscopy an

126 We use fluorescence recovery after photobleaching, fluorescence correlation spectroscopy, a

127 tion spectroscopy to quantify the diffusion, photobleaching, fluorescence intermittency, and photocon

128 agellar transport particles, or reduction of photobleaching for live microtubule imaging.

129 Moreover, fluorescence recovery after photobleaching (FRAP) analysis demonstrated that exposur

130 ealed ghosts and fluorescence recovery after photobleaching (FRAP) analysis of actin filament mobilit

131 determined using fluorescence recovery after photobleaching (FRAP) and binding, which is widely used

132 s we show, using fluorescence recovery after photobleaching (FRAP) and fluorescence anisotropy measur

133 ical techniques, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spect

134 Fluorescence recovery after photobleaching (FRAP) and fluorescence correlation spect

135 -PEO) film using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking (SMT)

136 Using fluorescence recovery after photobleaching (FRAP) and single-molecule tracking in hu

137 chia coli, using Fluorescence Recovery after Photobleaching (FRAP) and Total Internal Reflection Fluo

138 ation (FDAP) and fluorescence recovery after photobleaching (FRAP) are well established approaches fo

139 oscopy (FCS) and fluorescence recovery after photobleaching (FRAP) are widely used methods to determi

140 y was to develop fluorescence recovery after photobleaching (FRAP) as a technique to accurately and r

141 reased claudin 4 fluorescence recovery after photobleaching (FRAP) dynamics in response to inflammato

142 Fluorescence recovery after photobleaching (FRAP) experiments confirmed the differen

143 minutes, whereas fluorescence recovery after photobleaching (FRAP) experiments employing overexpressi

144 method based on fluorescence recovery after photobleaching (FRAP) for determining how many reaction

145 es by monitoring fluorescence recovery after photobleaching (FRAP) in transgenic zebrafish with GFP-t

146 Fluorescence recovery after photobleaching (FRAP) is a well-established experimental

147 Fluorescence recovery after photobleaching (FRAP) is a widespread technique used to

148 Fluorescence recovery after photobleaching (FRAP) is an excellent tool to measure th

149 scent probe from Fluorescence Recovery After Photobleaching (FRAP) measurements assumes bleaching wit

150 Fluorescence recovery after photobleaching (FRAP) microscopy is used to probe the di

151 were analyzed by fluorescence recovery after photobleaching (FRAP) microscopy.

152 emonstrated using fluorescent recovery after photobleaching (FRAP) monitoring displacement of GFP-BAZ

153 as studied using fluorescence recovery after photobleaching (FRAP) of GFP-Galphas.

154 Fluorescence recovery after photobleaching (FRAP) of labeled protein demonstrated th

155 experiments and fluorescence recovery after photobleaching (FRAP) of SC junctions in utricles from m

156 Interestingly, fluorescence recovery after photobleaching (FRAP) results indicated that NKKY101 mut

157 ell imaging with fluorescence recovery after photobleaching (FRAP) revealed that the population of Ga

158 Notably, fluorescence recovery after photobleaching (FRAP) shows that YscQ exchanges between

159 Fluorescence recovery after photobleaching (FRAP) studies indicate that like H1, bin

160 ssed receptor by fluorescence recovery after photobleaching (FRAP) to demonstrate that endoglin forms

161 cribe the use of fluorescence recovery after photobleaching (FRAP) to probe chain mobility in reversi

162 scopy (FCCS) and fluorescence recovery after photobleaching (FRAP) we found that the integrin adhesom

163 We performed fluorescent recovery after photobleaching (FRAP), quantitative RT-PCR, and whole ce

164 Using fluorescence recovery after photobleaching (FRAP), we demonstrate that adherens junc

165 rc together with fluorescence recovery after photobleaching (FRAP), we find that Src significantly re

166 Performing acceptor-photobleaching FRET studies with receptors 1, 2, and 4,

167 Perturbation of equilibrium distributions by photobleaching has also been developed into a robust met

168 Single-molecule photobleaching has emerged as a powerful non-invasive ap

169 ncluding inverse fluorescence recovery after photobleaching (iFRAP) and photoactivatable probes, coup

170 Fluorescence recovery after photobleaching imaging reveals that the S561A mutant sho

171 e complex can be revealed by single-molecule photobleaching imaging.

172 Photoconversion, photobleaching, immunofluorescence and super-resolution

173 n trigger autofluorescence, photoxicity, and photobleaching, impairing their use in vivo.

174 uorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment.

175 scent signals in the presence of fluorophore photobleaching in a solid surface bioassay.

176 butable to the absence of phototoxicity, and photobleaching in bioluminescent imaging, combined with

177 been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful

178 We show using fluorescence recovery after photobleaching in hippocampal neurons that the majority

179 stress by using fluorescence recovery after photobleaching in proplatelets with fluorescence-tagged

180 s and studies of fluorescence recovery after photobleaching in respiratory mucus showed that mechanis

181 Fluorescent recovery after photobleaching in slices from VGLUT1(Venus) knock-in mic

182 the technique of fluorescence recovery after photobleaching indicated that hsc70 is required for the

183 characterized by fluorescence recovery after photobleaching, indicates a nominally single average dif

184 Photobleaching is a major limitation of superresolution

185 Localization by stepwise photobleaching is especially suited for measuring nanome

186 theoretically that speckle imprinting using photobleaching is optimal when the laser energy and fluo

187 We confirm that STED Photobleaching is primarily caused by the depletion ligh

188 dard for experiments in which recovery after photobleaching is used to measure lateral diffusion.

189 labels (i.e., maximum emitted photons before photobleaching) is a critical requirement for achieving

190 ticles in a region of interest by repeatedly photobleaching its boundary.

191 rom quantitative analysis of single-molecule photobleaching kinetics without using SPT.

192 ameters were optimized to deliver 23.8 mJ of photobleaching light energy at a pulse width of 6 msec a

193 st, we visualized whole eisosomes and, after photobleaching, localized recruitment of new Pil1p molec

194 s in cancer phototherapy is often limited by photobleaching, low tumor selectivity, and tumor hypoxia

195 In addition, their strong resistance to photobleaching makes them suitable for long duration or

196 Fluorescence recovery after photobleaching measurements revealed that single mismatc

197 Fluorescence recovery after photobleaching measurements showed that both proteins co

198 her supported by fluorescence recovery after photobleaching measurements, which showed that, at heter

199 Our FRAP (fluorescence recovery after photobleaching) measurements showed that the complexes r

200 were thermo-unstable and exhibited abnormal photobleaching, metarhodopsin II decay, and G protein ac

201 ne by using FRET microscopy and the acceptor photobleaching method.

202 cted by dye concentration, light scattering, photobleaching, micro-viscosity, temperature, or the mai

203 via multiphoton fluorescence recovery after photobleaching (MP-FRAP) of injected FITC-BSA, a 32.6% d

204 vant information that may be acquired before photobleaching occurs.

205 ed probe DNA on these surfaces is unlabeled, photobleaching of a probe label is not an issue, allowin

206 luorescence and reduce photocarbonization or photobleaching of analytes.

207 In contrast to NAP SOA, the photobleaching of BrC material produced by the reaction

208 of reactive oxygen species, and potentially photobleaching of DOM.

209 sensitive cells with no phototoxicity and no photobleaching of fluorescent biomarkers.

210 toactivation and fluorescence recovery after photobleaching of fluorescently tagged AMPAR to show tha

211 ess this question, we performed quantitative photobleaching of GFP-tagged AtCESA3-containing particle

212 ic receptor (M2R) and Gi1 by single-particle photobleaching of immobilized complexes.

213 ong-term detection is partially prevented by photobleaching of organic fluorescent probes.

214 lid phase micro extraction (SPME-GC-MS), and photobleaching of photosensitizers in milk (riboflavin,

215 Photobleaching of rhodopsin function prevents accumulati

216 ation, the fundamental molecular event after photobleaching of rhodopsin is the recombination reactio

217 hat PKM2 phosphorylation is signaled through photobleaching of rhodopsin.

218 s technique remain present such as the rapid photobleaching of several types of organic fluorophores

219 Stepwise photobleaching of SpoIVFB fused to a fluorescent protein

220 the vesicle lumen domain of Sb2, and perform photobleaching of YpH fluorophores.

221 rtant, as many dyes suffer from either rapid photobleaching or high nonspecific staining.

222 scopy as well as fluorescence recovery after photobleaching or photoswitching, and observed significa

223 , a completely different, oxygen-independent photobleaching pathway was found to take place.

224 ted a reduced activation rate and an altered photobleaching pattern.

225 The photobleaching patterns of eGFP-M2R were indicative of a

226 n the quantification of photodarkening (PD), photobleaching (PB) and transient PD (TPD) in a-Ge(x)As(

227 tical properties to fluoresce with near-zero photobleaching, photoblinking and background autofluores

228 rastructure, and fluorescence recovery after photobleaching/photoconversion experiments showed that t

229 ted polymer to avoid leakage or differential photobleaching problems existed in other nanoprobes.

230 , incomplete saturation of binding sites, or photobleaching produces stochastic mixtures.

231 aging, including fluorescence recovery after photobleaching, provided further support for the role of

232 Single-molecule fluorescence recovery after photobleaching provides direct measurement of elongation

233 hotophysical parameters of the probe such as photobleaching quantum yield, count rate per molecule, a

234 This includes photobleaching, quenching, and the formation of non-emis

235 In this paper, we show that the photobleaching rate in STED microscopy can be slowed dow

236 tive redox reactions that contributed to the photobleaching rate were studied over a wide temperature

237 ter simulations, fluorescence-recovery-after-photobleaching recovery times of both fused and single-m

238 her increasing the linear scanning speed for photobleaching reduction in STED microscopy.

239 uce a method called smPReSS, single-molecule photobleaching relaxation to steady state, that infers e

240 Yet, the mechanisms that govern photobleaching remain poorly understood.

241 Photobleaching remains a limiting factor in superresolut

242 ion coefficient, low quantum yield, and high photobleaching resistance.

243 TIR-fluorescence recovery after photobleaching revealed a significant recovery of tPA-ce

244 The use of fluorescence recovery after photobleaching revealed an increase in plasma membrane f

245 Fluorescence recovery after photobleaching revealed that the viscosity of CF ASL was

246 In addition, fluorescence recovery after photobleaching revealed that, although the antiholin irs

247 Fluorescence recovery after photobleaching reveals reversible changes in the level o

248 s include mechanical uncertainties, specimen photobleaching, segmentation, and stitching inaccuracies

249 xperiments using fluorescence recovery after photobleaching show that human FZD4 assembles-in a DVL-i

250 Stepwise photobleaching showed that CaMKII formed oligomeric comp

251 The analysis of fluorescence recovery after photobleaching showed that the fluxes of dye molecules i

252 Fluorescence recovery after photobleaching shows that TSSC1 is required for efficien

253 fluorescence in the NIR2 regime and lack of photobleaching, single-walled carbon nanotubes (SWNTs) a

254 gy to perform single-molecule recovery after photobleaching (SRAP) within dense macromolecular assemb

255 ty traces over time is the quantification of photobleaching step counts.

256 sian analysis of images collected during the photobleaching step of each plane enabled lateral superr

257 Although photobleaching steps are often detected by eye, this met

258 Our method is capable of detecting >/=50 photobleaching steps even for signal-to-noise ratios as

259 orithms, it is possible to reliably identify photobleaching steps for up to 20-30 fluorophores and si

260 ties have created a challenge in identifying photobleaching steps in a time trace.

261 factors can limit and bias the detection of photobleaching steps, including noise, high numbers of f

262 ta receptor with fluorescence recovery after photobleaching studies on the lateral diffusion of a coe

263 Fluorescence recovery after photobleaching studies showed that these RTNLBs are mobi

264 linked Orai1 concatemers and single-molecule photobleaching suggest that channels assemble as tetrame

265 A-wave recovery compared with WT mice after photobleaching, suggesting a delayed dark adaptation.

266 Using fluorescence recovery after photobleaching technique, diffusion coefficients D of fl

267 Zea mays) by the fluorescence recovery after photobleaching technique.

268 wo-photon absorption cross-section and rapid photobleaching tendency, their applications in two-photo

269 ters are frequently degraded by blinking and photobleaching that arise from poorly passivated host cr

270 CDOM origin (terrestrial versus marine) and photobleaching that controls variations in AQYs, with a

271 sion characteristics, including blinking and photobleaching that limit their utility and performance.

272 excitation zone by sequentially imaging and photobleaching the fluorescent molecules.

273 w blinking characteristics due to reversible photobleaching, the blinking of GNPs seems to be stable

274 After photobleaching, the EB1 signal at the flagellar tip reco

275 Irradiation with laser light above the photobleaching threshold induces photonic confinement po

276 The noise properties in a photobleaching time trace depend on the number of active

277 t a new Bayesian method of counting steps in photobleaching time traces that takes into account stoch

278 Here, we applied single-molecule photobleaching to analyze the oligomeric state of an end

279 a procedure for fluorescence recovery after photobleaching to examine dye leakage through bacterial

280 GJs by applying fluorescence recovery after photobleaching to GJs formed from connexins fused with f

281 the anthropogenic source shows a shift from photobleaching to photohumification denoted by an increa

282 we use fluorophore localization imaging with photobleaching to probe the structure of EGFR oligomers.

283 including using chemical cleavage instead of photobleaching to remove fluorescent signals between con

284 st successful application of single-molecule photobleaching to resolve drug-induced and domain-depend

285 at conventional fluorophores undergo minimal photobleaching under standard illumination in the FDS.

286 The technique of Fluorescence Recovery After Photobleaching was applied for the first time on real ch

287 ured by 2-photon fluorescence recovery after photobleaching, was not affected just after cardiorespir

288 Using fluorescence recovery after photobleaching we show here that beta-arrestin is requir

289 By fluorescence recovery after photobleaching, we demonstrate a high mobility and fast

290 rgy transfer and fluorescence recovery after photobleaching, we demonstrate that arrestin-3 dissociat

291 plete recovery of dextran fluorescence after photobleaching, we demonstrated that the actin ring-asso

292 Through live-cell photobleaching, we find rapid binding kinetics between P

293 Using fluorescence loss in photobleaching, we find that the endoplasmic reticulum (

294 urthermore using fluorescence recovery after photobleaching, we found that FAK inhibition increased t

295 ited in observation time and photon count by photobleaching, we present a description of the sources

296 Using fluorescence recovery after photobleaching, we show that an ACTN4 mutation that caus

297 Using fluorescence recovery after photobleaching, we show that the ER chaperone Kar2/BiP f

298 lapse imaging of fluorescence recovery after photobleaching, we show that X11alpha is present in a mo

299 or wood smoke BrC, both photoenhancement and photobleaching were observed.

300 per molecule, the saturation intensity, the photobleaching yield, and, crucially, management of brig