ライフサイエンスコーパス: photocycle

1 es from TMR to the carotenoid throughout the photocycle.

2 CBD could be used for further studies of the photocycle.

3 e active cofactor and complete the catalytic photocycle.

4 ically to close an oxygen-to-water reduction photocycle.

5 These steps conform to those in PYP's photocycle.

6 p for one-way proton transfer for the entire photocycle.

7 inherent entropy-driven late steps in the BR photocycle.

8 domain by itself can complete the Pr to Pfr photocycle.

9 ational response in the intermediates in the photocycle.

10 roton transfer reactions in the fluorescence photocycle.

11 tions is the primary step in the BLUF domain photocycle.

12 than WT) are due to unrelated events in the photocycle.

13 to reveal the role of residue Y98 in the PYP photocycle.

14 5, which remains unprotonated throughout the photocycle.

15 cysteine 62 composing a critical part of the photocycle.

16 c and structural changes in the fluorescence photocycle.

17 phototransformation and to the fluorescence photocycle.

18 with a dominant accumulation of M during the photocycle.

19 zed blue bR containing all the lipids has no photocycle.

20 he early or late M state of the protonmotive photocycle.

21 ty limitations that do not change during the photocycle.

22 groups along the transport chain during the photocycle.

23 cavities from residue rearrangements in the photocycle.

24 l change during the BR-->M portion of the BR photocycle.

25 h a concomitant increase in the speed of the photocycle.

26 ranslocation rate attributable to its slower photocycle.

27 s of M-intermediate in its bacteriorhodopsin photocycle.

28 tion implicating involvement of Tyr21 in the photocycle.

29 hain during key conformational states of the photocycle.

30 critical intermediates of the proton-motive photocycle.

31 helix F in the M and N intermediates of the photocycle.

32 n transfer process in the later phase of the photocycle.

33 We measured pH and isotope effects on the photocycle.

34 ctural transformations achieved later in the photocycle.

35 rs, and a subnanosecond component during the photocycle.

36 ncy, the earliest M intermediate (M1) of the photocycle.

37 in the ground state that changes during the photocycle.

38 alized kinetic model for the interprotein ET photocycle.

39 bserved in bacteriorhodopsin (bR) during its photocycle.

40 ut also rotates counter-clockwise during the photocycle.

41 ignificant perturbation of R82 during the bR photocycle.

42 changes in the protonation state during the photocycle.

43 active site of channelrhodopsin-2 during the photocycle.

44 ce areas of OCP to the solvent following the photocycle.

45 photointermediates in the bacteriorhodopsin photocycle.

46 ulation of radical intermediates in the BLUF photocycle.

47 onal isomerizations are also involved in the photocycle.

48 the reprotonation step explaining the slower photocycle.

49 nor for chromophore reprotonation during the photocycle.

50 blue and UV-A regions and shows a truncated photocycle.

51 acterization of the light-driven half of the photocycle.

52 ately prior to electron transfer in the BLUF photocycle.

53 ept a proton from the Schiff base during the photocycle.

54 he superposition of two independent parallel photocycles.

55 ery phenomena are inherent properties of the photocycles.

56 terms of contributions from the two parallel photocycles.

57 intained in a standard 12:12 hour light-dark photocycle (30 lux during the day and 0 lux at night).

58 were housed in a 12:12 hour light-dim light photocycle (30 lux during the day and 3 lux at night).

59 g decay of the O state, the last step of the photocycle, a proton is transferred from Asp85 to proton

60 product is apparently a side reaction of the photocycle, a response to high pH, caused by alteration

61 The M intermediate in the wild-type photocycle accumulates only at high pH, with an apparent

62 uctural rearrangements of OCP throughout the photocycle and a completely novel approach to the study

63 of conformation and configuration of several photocycle and conducting/nonconducting states need to b

64 These key dynamics define the repair photocycle and explain the underlying molecular mechanis

65 f such a low pK(a) is an acceleration of the photocycle and high pump turnover at high light intensit

66 reatly improved our understanding of the OCP photocycle and interaction with phycobilisomes and the f

67 The photocycle and molecular mechanism of animal cryptochrom

68 ompletely novel approach to the study of its photocycle and non-photochemical quenching.

69 und state absorption properties, kinetics of photocycle and of proton release, and uptake in the muta

70 of a 1697 cm(-1) (+) difference band in both photocycle and phototransformation intermediates is a sp

71 les conformational switching from the flavin photocycle and prevents Vivid from sending signals in Ne

72 sins and the effects of R82 mutations on the photocycle and proton release.

73 se mutations is of crucial importance to the photocycle and suggest that the hinge-bending properties

74 iff base to its counterion Asp-97 during the photocycle and the acid-induced protonation of Asp-97 in

75 zation of purified protein revealed that the photocycle and the transport mechanism of PsChR1 differ

76 r flavin-tryptophan and flavin-ascorbic acid photocycles and the closely related intramolecular flavi

77 he molecule are opened and closed during the photocycle, and affinity-switch models, which focus on c

78 ds a flavin cofactor, undergoes a reversible photocycle, and displays increased ATPase and autophosph

79 lysis revealed six distinct states in the NR photocycle, and we describe their spectral properties an

80 function independently of both rod and cone photocycles, and that apo-melanopsin has a strong prefer

81 duration may be long enough to resolve PYP's photocycle (approximately 0.3 s).

82 The initial nanoseconds of the PYP photocycle are examined using time-dependent density fun

83 giving rise to these continua during the bR photocycle are proposed.

84 ase proton transfer reactions during the BPR photocycle as previously shown for GPR, but BPR contains

85 luding conformer interexchangeability in the photocycle as shown here, is likely a significant factor

86 he signaling state intermediates of the SRII photocycle, as well as an influence of HtrII on the hydr

87 the N to O reaction of the bacteriorhodopsin photocycle, Asp-96 is protonated from the cytoplasmic su

88 The complex structural changes during the photocycle at ambient temperature are displayed in a mov

89 wise conserved WXE motif, W389A, changes the photocycle at intermediate R2 and causes an alternative

90 arallel model for the bacteriorhodopsin (BR) photocycle at neutral pH and a temperature near 20 degre

91 or complex formed in the pB state of the PYP photocycle at pH 4.

92 Using an ultrafast transition of the bR photocycle (BR-K), we achieved high-speed (nanosecond) l

93 Starting from M, a photocycle branching occurs involving (i) a direct M -->

94 We investigated the complete PYP photocycle by acquiring time-resolved small and wide-ang

95 We studied the kinetics of the OCP photocycle by monitoring changes in its absorption spect

96 These results suggest that the melanopsin photocycle can function independently of both rod and co

97 tion 101 as a mediator of both allostery and photocycle catalysis that can impact organism physiology

98 s for the variation of the properties of the photocycle caused by changes in actinic light intensity.

99 bits predominantly an all-trans retinylidene photocycle containing a deprotonated Schiff base species

100 early intermediate of the bacteriorhodopsin photocycle, contains the excess free energy used for lig

101 Below 195 K, the bacteriorhodopsin photocycle could not be adequately described with expone

102 lent system for understanding how changes in photocycle dynamics affect signaling by PYPs.

103 playing significant roles in the control of photocycle dynamics and, by comparison to other sensory

104 ant step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates

105 e polypyridyl Ni(II) dichloride, closing the photocycle for H2 generation from HCl.

106 es 1-9 permit the construction of a complete photocycle for the photogeneration of H2 by dirhodium df

107 ermediate of the wild-type bacteriorhodopsin photocycle formed by actinic illumination at 230 K has b

108 , and most of the main features of the known photocycle from very early M to the return to the restin

109 We show that parallel transformations in the photocycle have a structural origin, and we report on th

110 difference spectra of the halorhodopsin (hR) photocycle have been collected from 3 micros to 100 ms i

111 The dynamics of the PYP photocycle have been studied using time-resolved optical

112 Under short-day (SD) photocycles, hypocotyl elongation is maximal at dawn, be

113 We produced the L intermediate of the photocycle in a bacteriorhodopsin crystal in photo-stati

114 We describe a novel orange/green photocycle in one of these CBCRs, NpF2164g7.

115 trans-p-coumaric acid (trans-CA) triggers a photocycle in photoactive yellow protein that ultimately

116 at His-75 undergoes deprotonation during the photocycle in the proton-pumping (high pH) form of PR, a

117 We propose a model photocycle in which Z/ E photoisomerization of the 15/16

118 sponse to entrainment to thermocycles versus photocycles in constant temperature.

119 ding AnPixJ and NpR6012g4, exhibit red/green photocycles in which the 15Z photostate is red-absorbing

120 ructural transitions unveiled during the PYP photocycle include trans/cis isomerization, the breaking

121 The L to M reaction of the bacteriorhodopsin photocycle includes the crucial proton transfer from the

122 mational changes are detected during the ASR photocycle including in the transmembrane helices E and

123 rstanding of how molecular events during the photocycle, including the retinal trans-cis isomerizatio

124 s region) as well as short-circuiting of the photocycle, increasing its duty cycles.

125 the 14 kDa protein increased the rate of the photocycle, indicating physical interaction with the mem

126 In vivo, WCC has a long period photocycle, indicating that it cannot be efficiently use

127 ng the PYPs studied to date in that it has a photocycle initiated from a dark-adapted state with a pr

128 t, although the gateway may be important for photocycle initiation, its role in recovery to the groun

129 s protonation of the Schiff base in a 13-cis photocycle intermediate (M right harpoon over left harpo

130 hese structures provide a model for the last photocycle intermediate (O) of bacteriorhodopsin, in whi

131 d its ability to photoisomerize to the first photocycle intermediate are insensitive to most single m

132 In contrast, for the partially unfolded pB photocycle intermediate containing cis-pCA, unfolding-in

133 The calculated excitation energy of the photocycle intermediate cryogenically trapped in a cryst

134 iously unknown light-sensitive, blue-shifted photocycle intermediate L (lambdamax = 495 nm), which is

135 -1) vibrational difference band due to the M photocycle intermediate of (14)N-Arg-bR loses substantia

136 cies make it a poor model for the long-lived photocycle intermediate, I(2), which undergoes more mode

137 onstants for the formation of the subsequent photocycle intermediates (I(0)(double dagger) and I(1)),

138 ical calculations that the obtained putative photocycle intermediates are in close agreement with exp

139 similar spectral shifts, indicating similar photocycle intermediates as GtACR1 in detergent micelles

140 c-angle spinning NMR spectra of cryo-trapped photocycle intermediates of bacteriorhodopsin (bR) by a

141 ier transform infrared (FTIR) studies of the photocycle intermediates of bacteriorhodopsin at cryogen

142 was utilized to monitor the evolution of the photocycle intermediates of bR reconstituted in nanodisc

143 acteriorhodopsin provides models for several photocycle intermediates of the wild-type protein in whi

144 tein can be linked to the elongation of late-photocycle intermediates studied by flash photolysis.

145 Notably, the photocurrent and various photocycle intermediates were recorded simultaneously.

146 as not been possible to correlate any of its photocycle intermediates with a relevant signaling state

147 lattice, the earliest bacteriorhodopsin (BR) photocycle intermediates, and divalent cations in the co

148 ures and spectroscopic measurements on early photocycle intermediates.

149 to calculate excitation energies to identify photocycle intermediates.

150 The photocycle involves changes of one or both of these prot

151 ophore that absorbs blue light, initiating a photocycle involving a series of conformational changes.

152 in terms of the radical pair mechanism and a photocycle involving the recently discovered fourth tryp

153 copy to show that the key step in the repair photocycle is acyclic proton transfer between the enzyme

154 membrane during the L-to-M transition of the photocycle is addressed by including as pH-titratable si

155 This implies that, if the NR photocycle is associated with proton transport, it has a

156 Dynamics of the AppA photocycle is discussed in view of the currently solved

157 The deprotonation of Glu-90 during the photocycle is elucidated by time-resolved FTIR spectrosc

158 ates of the photoactive yellow protein (PYP) photocycle is examined via ab initio vibrational self-co

159 The step critical to entry into the photocycle is identified as flipping of the carbonyl gro

160 Previous work has shown that the melanopsin photocycle is independent of that used by rhodopsin.

161 onstrate that the melanopsin-dependent ipRGC photocycle is independent of the visual retinoid cycle.

162 The cry1 photocycle is initiated by light absorption by its FAD c

163 transfer reactions, implying that the 13-cis photocycle is of minor functional relevance for ChR2.

164 ltimately developed in the first half of the photocycle is probably responsible for enforcing vectori

165 latively high (7.1), the proton-transporting photocycle is produced only at alkaline pH.

166 The photocycle is significantly elongated mainly due to an e

167 It had previously been shown that the photocycle is slowed by as much as 3 orders of magnitude

168 Its photocycle is studied extensively under different pH con

169 ar or cytoplasmic surface, respectively, the photocycle is the same as in the single mutant R82A with

170 s at physiological pH, the net effect of BPR photocycling is to generate proton currents dominated by

171 main can, by itself, complete the Pr --> Pfr photocycle, it should now be possible to determine the s

172 Our data show that photocycle kinetic parameters are sufficient to explain

173 le absorption maxima (530 versus 490 nm) and photocycle kinetics ( approximately 20 versus approximat

174 Y98Q mutant of PYP has a major effect on the photocycle kinetics ( approximately 40 times slower reco

175 ge differences in the pH dependence of their photocycle kinetics and in the pK(a) of Asp76 that contr

176 droxyls and the surrounding protein regulate photocycle kinetics and stabilize the LOV active site fr

177 Both mutants have slightly perturbed photocycle kinetics and, similar to the R52A mutant, are

178 Following illumination at 358 nm, photocycle kinetics are characterized at pH 7.0 by a sma

179 has the same absorption spectrum and similar photocycle kinetics as full length Ppr, but a blue-shift

180 etation of the photocurrents in terms of the photocycle kinetics indicates that the O states in both

181 The photocycle kinetics is significantly altered by substitu

182 To study the lipid-composition dependence of photocycle kinetics of bacteriorhodopsin (bR), transient

183 ransient absorption contours showed that the photocycle kinetics of bR was significantly retarded and

184 The photocycle kinetics of Platymonas subcordiformis channel

185 he effects of BC loop modifications onto the photocycle kinetics of proteorhodopsin.

186 and that pH-induced changes observed in the photocycle kinetics of PYP cannot be caused by changes i

187 The correlation of photocycle kinetics with side-chain conformation of the

188 asurement of the photochemical activity, the photocycle kinetics, and the absorption spectra at vario

189 Here we present the crystal structure, photocycle kinetics, association properties, and spectro

190 een identified as a possible key mediator of photocycle kinetics, despite the lack of ordered water i

191 ce of the chromophore's negative charge, the photocycle kinetics, the signaling mechanism, and the pr

192 nding network, while affording tunability of photocycle kinetics.

193 function as an intrinsic base to accelerate photocycle kinetics.

194 l bacteriorhodopsin structure, function, and photocycle kinetics.

195 This occurs in the transition from the photocycle late M state to the N state.

196 f the retinal chromophore and the subsequent photocycle, leading to the formation (on-gating) and dec

197 Environmental time cues, such as photocycles (light/dark) and thermocycles (warm/cold), s

198 riorhodopsin and the mean decay times of the photocycle M-state intermediates.

199 lly detailed description of the complete PYP photocycle, made possible by time-resolved crystal and s

200 Transitions in the photocycle, measured with laser induced transient absorp

201 ers, BD predictions were verified through ET photocycle measurements enabled by Zn-deuteroporphyrin s

202 erization rate and thermal activation of the photocycle, melanopsin turns out to be a highly sensitiv

203 We will discuss an extended photocycle model of this mutant on the basis of spectros

204 s provide the same kinetic parameters of the photocycle, namely, the kinetics of OCP relaxation in da

205 Here, we have analyzed the photocycle of a fast-cycling ChR2 variant (E123T mutatio

206 ecord, in D2 O, the complete proton transfer photocycle of avGFP, and two mutants (T203V and S205V) w

207 The role of proline residues in the photocycle of bacteriorhodopsin (bR) is addressed using

208 The proton pump photocycle of bacteriorhodopsin (bR) produces photocurre

209 In the photocycle of bacteriorhodopsin (BR), the first proton m

210 In the photocycle of bacteriorhodopsin at pH 7, a proton is eje

211 In the photocycle of bacteriorhodopsin at pH 7, proton release

212 The L intermediate in the proton-motive photocycle of bacteriorhodopsin is the starting state fo

213 trum of the K intermediate in the ion-motive photocycle of bacteriorhodopsin.

214 ed as compared to other intermediates in the photocycle of both PYP and Bph, we interpret this as for

215 regime may be functionally important for the photocycle of bR, and protein-lipid interactions are mot

216 The primary event in the photocycle of CaChR1 involves an isomerization of the pr

217 namics associated with the first step of the photocycle of CaChR1.

218 These results show that the photocycle of DNA repair by photolyase is through a radi

219 uced reactions play an important role in the photocycle of fluorescent proteins from the green fluore

220 nching reactions in fSRI, not evident in the photocycle of native SRI.

221 used this capability to track the reversible photocycle of photoactive yellow protein (PYP) following

222 time-resolved crystallographic data for the photocycle of photoactive yellow protein.

223 the pR, pB', and pB intermediates during the photocycle of photoactive yellow protein.

224 Our study provides novel insight into the photocycle of PR and also demonstrates the power of DNP-

225 s that 124 mutants retain the characteristic photocycle of PYP, but that the majority of substitution

226 racterized the proton transfer events in the photocycle of ReaChR and describe their relevance for it

227 The light-driven photocycle of rhodopsin begins the photoreceptor cascade

228 The photocycle of the bacterial blue-light photoreceptor, ph

229 , we present spectroscopic evidence that the photocycle of the C128T mutant involves three different

230 Continued activation of the photocycle of the dim-light receptor rhodopsin leads to

231 08Q mutants, and the effects of azide on the photocycle of the E108Q mutant, at low and high pH.

232 The fluorescence photocycle of the green fluorescent protein is functiona

233 To date, the light-induced photocycle of the phot1 LOV2 protein is known to involve

234 which the M intermediate is observed in the photocycle of the protein solubilized in detergent [dode

235 c residues in proton transfer, and as in the photocycle of the wild-type protein, no intermediate wit

236 To investigate whether the altered photocycle of Y98Q is due to this repositioning of M100

237 with variable vectoriality, we compared the photocycles of D97N and E108Q mutants, and the effects o

238 ght very significant differences between the photocycles of PixD and AppABLUF, despite their sharing

239 derived a two-cycle model that describes the photocycles of PsChR2.

240 plex than those reported for the red/far-red photocycles of the related phytochrome photoreceptors.

241 nstructed, for the rates of reactions in the photocycles of wild-type bacteriorhodopsin and several s

242 ons of functional and shunt states in the bR photocycle provide a preview of the mechanistic insights

243 c resolution description of the complete PYP photocycle provides a framework for understanding signal

244 The photocycle rate increases with increasing temperature, b

245 Furthermore, the photocycle rate is dependent on electric potential gener

246 Only E46Q PYP exhibits a greatly accelerated photocycling rate.

247 ent ocean depths show that the difference in photocycle rates between GPR and BPR as well as their di

248 of retinal provides evidence that the 13-cis photocycle recovers in 1 ms.

249 98L, and Y98A) have differing effects on the photocycle recovery, presumably due to a variable distor

250 ger changes that occur early and late in the photocycle, respectively.

251 erates a long-lived photointermediate of its photocycle, S(373), and an attractant phototactic respon

252 The proposed photocycle scheme and these rate constants predict well

253 A photocycle scheme proposed earlier places the two main p

254 It appears from the photocycle scheme, and the numerical values of rate cons

255 duced protein-protein electron transfer (ET) photocycle should exhibit differential responses to dyna

256 MvirPHY1 exhibits a red-far-red photocycle similar to those seen in other streptophyte a

257 the chromophore conformation in the various photocycle states are still lacking.

258 cular dynamics (MD) simulations of transient photocycle states.

259 Due to their slow photocycle, structural alterations of these proteins can

260 abrupt change in the kinetic behavior of the photocycle takes place.

261 lso observed in parallel measurements of the photocycle (tau(3) and tau(4)).

262 e-shifted absorbance and considerably slower photocycle than E-PYP.

263 Blue light excitation of AppA induces a photocycle that is characterized by a long-lived red-shi

264 ntly bound within PYP as the first step in a photocycle that results in the host bacterium moving awa

265 state in the dark, effectively completing a photocycle that serves as a molecular switch to control

266 erties of the different intermediates in the photocycle, the possible origins of the polarizable prot

267 ainment of the Arabidopsis thaliana clock to photocycles, the determinants of thermoperception and en

268 1 and M2 reveals the principal switch in the photocycle: the change of the angle of the C15=NZ-CE pla

269 intermediate states in the bacteriorhodopsin photocycle, this study also suggests the overall design

270 D85 is necessary at the end of the wild-type photocycle to avoid the generation of nonfunctional C [d

271 may be involved in the adaptation of the BR photocycle to different levels of actinic light.

272 gly affected slow channel closing slowed the photocycle to the same extent.

273 acid-denatured state of PYP mimics the last photocycle transition in PYP.

274 The kinetics of the last photocycle transition vary by more than 4 orders of magn

275 e on light-induced proton release and on the photocycle transitions associated with proton transfer.

276 of proteins that do not enter the signaling photocycle, TRCD provides strong evidence that the carbo

277 harge of voltage and concomitant decrease in photocycle turnover time.

278 ctron transfer is not part of the photolyase photocycle under physiological conditions.

279 s), suggesting that the melanopsin-dependent photocycle utilizes RPE65 and Lrat.

280 The bacteriorhodopsin (bR) photocycle was followed by use of time-resolved Fourier-

281 The photocycle was monitored by time-resolved FTIR between 1

282 s and pathways of the bacteriorhodopsin (BR) photocycle was reviewed.

283 The photocycle was studied with flash photolysis and time-re

284 reaction is part of the DpCry1 physiological photocycle, we mutated the Trp residue that acts as the

285 tion steps during the bacteriorhodopsin (BR) photocycle were determined by time-resolved photopressur

286 Early intermediates of bacteriorhodopsin's photocycle were modeled by means of ab initio quantum me

287 Proton release and uptake during the photocycle were monitored with the pH-sensitive dye, pyr

288 een 260 and 298 K in the context of a simple photocycle, were found to be larger at low pH than at hi

289 ze rhodopsin and enhance the kinetics of the photocycle, whereas cholesterol has the opposite effect.

290 lin chromophore as the primary step in their photocycle, which consists of reversible photoconversion

291 Both LOV1 and LOV2 undergo a self-contained photocycle, which involves the formation of a covalent a

292 als several intermediates involved in PAiRFP photocycles, which all differ from that of the bacteriop

293 at Tyr8 and Gln50 result in the loss of the photocycle while a mutation of Met93 does not appreciabl

294 va light-oxygen-voltage domain 2 undergoes a photocycle with a conformational change involving the un

295 The purified pigment undergoes a photocycle with a prominent red-shifted intermediate who

296 , although only the former participates in a photocycle with a signaling M intermediate.

297 ue-light excitation, the protein undergoes a photocycle with different intermediates.

298 structural investigations of the phytochrome photocycle with time-resolved SFX.

299 hodopsin, HR520 Cl- and HR640, into a single photocycle, with a chloride-dependent equilibrium betwee

300 Such sensors exhibit a diverse range of photocycles, yet all share ground-state absorbance of ne