ライフサイエンスコーパス: photoincorporation

1 1 as the primary sites of [(3)H]Bz(2)choline photoincorporation.

2 identified as the sites of specific [3H]dTC photoincorporation.

3 ntiomer, CP-96,344 was a potent inhibitor of photoincorporation.

4 ith alphaTyr-190 the primary site of [3H]dTC photoincorporation and alphaCys-192 and alphaTyr-198 lab

5 T)10 protect the Klenow fragment from [3H]-1 photoincorporation, and TTP at a concentration approxima

6 was again observed as measured by the BisANS photoincorporation approach.

7 studies because it had the highest extent of photoincorporation, approximately 1%, of the three radio

8 about 5% and 1% of the efficiency of [3H]dTC photoincorporation at gamma Trp-55, the primary site of

9 The efficiency of photoincorporation at saturation of binding sites was de

10 the agonist carbamylcholine, consistent with photoincorporation at the nAChR lipid-protein interface.

11 334 enhances GluT1.HA.H6 [gamma-32P]azidoATP photoincorporation but blocks acute modulation of net su

12 e allowed us to visualize the three sites of photoincorporation by molecular modeling.

13 Inhibition of [(125)I]TID analog photoincorporation by NCAs (e.g. tetracaine) as well as

14 To determine the site(s) of photoincorporation by Ro15-4513, we affinity-purified (

15 d result of an increase in the efficiency of photoincorporation compared to the wild-type receptor.

16 o the fast desensitized state, the extent of photoincorporation decreasing only with the transition t

17 ed Bpa8-SP resulted in a marked reduction in photoincorporation efficiency compared to the wild-type

18 edo nAChR ion channel with the efficiency of photoincorporation enhanced in the presence of agonist a

19 To identify the sites of photoincorporation, gamma- and delta-subunits, isolated

20 Because the major site of flunitrazepam photoincorporation has been shown to be His102, we propo

21 In contrast, the extent of photoincorporation in the channel lumen at the conserved

22 entification of the sites of [(125)I]TIDBIBA photoincorporation in the deltaM2 segment indicate a com

23 not for [(125)I]TIDPC/16, there was enhanced photoincorporation in the E(2) conformation, and this co

24 nsmembrane domain accounted for the enhanced photoincorporation induced by a 10-ms agonist exposure b

25 he agonist sensitivity of [(125)I]TID analog photoincorporation into AChR subunits.

26 ty, and NCA inhibition of [(125)I]TID analog photoincorporation into AChR subunits.

27 , and R-mTFD-MPAB each inhibited [(3)H]AziPm photoincorporation into GABAAR subunits maximally by app

28 TP (but not AMP) enhances [3H]cytochalasin B photoincorporation into GluT1 while cytochalasin B (but

29 cytochalasin D) enhances [gamma-32P]azidoATP photoincorporation into GluT1.

30 Reduced pH enhances azidoATP photoincorporation into isolated red cell GluT1 but inhi

31 of diphenylhexatriene in lipid bilayers and photoincorporation into nAChR-rich membrane phospholipid

32 o nts 2604-12, leads to target site-specific photoincorporation into protein L2 and 23S rRNA nucleoti

33 d 50 S subunits leads to site-specific probe photoincorporation into proteins L15, L17, and L20, labe

34 2448-58, leads to target site-specific probe photoincorporation into proteins L2, L3, one or more of

35 nd 50S subunits leads to site-specific probe photoincorporation into proteins L2, the most highly lab

36 esponse curves for inhibition of [(125)I]TID photoincorporation into the AChR channel and a progressi

37 [(125)I]TID photoincorporation into the alphaBgTx-5-HT(3A)R subunit

38 Desensitization decreased photoincorporation into the delta-subunit and increased

39 Conformationally sensitive [(125)I]TID photoincorporation into the M5 and M6 segments does not

40 resulted in preferential [(3)H]azietomidate photoincorporation into the nAChR alpha and delta subuni

41 ing: 1) their ability to inhibit [(125)I]TID photoincorporation into the resting state channel; 2) th

42 e extent of [(125)I]TID and [(125)I]TIDPC/16 photoincorporation into these transmembrane segments was

43 ta > alpha approximately beta > gamma), with photoincorporation limited to the nAChR transmembrane do

44 human IGFBP-2 (rhIGFBP-2) complex indicated photoincorporation near the carboxyl terminus of rhIGFBP

45 quirement for magnesium, with no significant photoincorporation observed at concentrations of 1 up to

46 No photoincorporation occurred in the absence of UV light.

47 ntaphospho-(5')-adenosine (Ap5A) reduced the photoincorporation of 3-[(3)H]azioctanol by 75%.

48 inhibited the binding of [(3)H]tetracaine or photoincorporation of 3-trifluoromethyl-3-(m-[(125)I]iod

49 for the active site with a stoichiometry of photoincorporation of 75-80%.

50 Specific covalent photoincorporation of 8-azido-[alpha-32P]ATP into the 2-

51 dies showing concentration- and UV-dependent photoincorporation of [(3)H]atRA into PKCalpha, which wa

52 ed with [(3)H]tetracaine results in specific photoincorporation of [(3)H]tetracaine into each nAChR s

53 zyme form shifts the dose-response curve for photoincorporation of [3H]-1 into the Klenow fragment by

54 Additionally, the photoincorporation of [3H]-1 into the Klenow fragment ha

55 Photoincorporation of [3H]DAF into the AChR consisted of

56 elta-subunits that are the sites of specific photoincorporation of [3H]dTC.

57 Investigation of the BZ site after photoincorporation of [3H]flunitrazepam confirmed that b

58 Photoincorporation of [alpha-32P]-8-N3-ADP-HPD was inhib

59 unlabeled azidoATP enhances subsequent GluT1 photoincorporation of [gamma-32P]azidoATP.

60 ure of the AChR, we have mapped the sites of photoincorporation of a benzoic acid ester analogue of T

61 The site of photoincorporation of a third photoaffinity analogue of

62 Mass spectrometry showed photoincorporation of all three alcohols in PKCdelta C1B

63 alpha-crystallin significantly decreased the photoincorporation of bis-ANS to alpha-crystallin.

64 photolabeled receptor restricted the site of photoincorporation of Bpa(4)-SP to an amino acid within

65 Photoincorporation of flunitrazepam into a single popula

66 Photoincorporation of ligands into the benzodiazepine si

67 and ETD, we identified Glu73 as the site of photoincorporation of our neurosteroid ligand in the IMP

68 we have proteolytically mapped the sites of photoincorporation of the hydrophobic compounds 3-(trifl

69 Photoincorporation of the hydrophobic probe 1,1'-bi(4-an

70 Each ligand could enhance photoincorporation of the other, demonstrating allosteri

71 nstrated that nucleotide competitors inhibit photoincorporation of the photoaffinity analogues [gamma

72 Photoincorporation of this SP derivative was susceptible

73 (the effective concentration at 50% maximum photoincorporation) of about 0.74 microM.

74 The photoincorporation reaction was further used to determin

75 Within 1.5 ms, the fractional change in photoincorporation relative to the closed state decrease

76 The photoincorporation saturated at low concentrations of th

77 oromethyl-3-(m-[(125)I]iodophenyl) diazirine photoincorporation than the S(-)-enantiomers.

78 xtracts of bovine cortex, [(3)H]azietomidate photoincorporation was increased by GABA and inhibited b

79 Photoincorporation was maximized by prolonged irradiatio

80 Site-specific photoincorporation was observed for partially purified G

81 The photoincorporation was shown to be specific for the acti

82 r [(14)C]amobarbital binding nor [(125)I]TID photoincorporation, we conclude that these positively ch

83 No other sites of photoincorporation were observed despite 90% sequence co

84 at gamma Trp-55, the primary site of [3H]dTC photoincorporation within gamma-subunit.

85 the bedG(1)IGF-1.rhIGFBP-2 complex revealed photoincorporation within residues 212-227.