ライフサイエンスコーパス: photolabeling

1 , and top-down MS confirmed a single site of photolabeling.

2 and indinavir effectively protected against photolabeling.

3 ulator, which neither enhanced nor inhibited photolabeling.

4 econds after mixing, by use of time-resolved photolabeling.

5 imidodiphosphate afforded protection against photolabeling.

6 zyme, consistent with a 1:1 stoichiometry of photolabeling.

7 domains of microsomal PGHS-1 are subject to photolabeling.

8 roactive steroids inhibited etomidate analog photolabeling.

9 istance (CQR) affect the efficiency of AzBCQ photolabeling.

10 effect of phencyclidine (PCP) on [(125)I]TID photolabeling.

11 cid residues of the receptor for [125I]IACoc photolabeling.

12 Rs) were studied using electrophysiology and photolabeling.

13 Agonist enhanced the photolabeling 10-fold in a fragment containing the M1, M

14 Importantly, we did not detect significant photolabeling after deleting amino acid regions in Vps33

15 a site at the gamma-alpha subunit interface, photolabeling alphaM2-10 (alphaSer-252) and gammaMet-295

16 te); and (iii) at the gamma-alpha interface, photolabeling alphaM2-10'.

17 t sites within the alpha and delta subunits, photolabeling alphaVal-218 (alphaM1), deltaPhe-232 (delt

18 nds at the extracellular end of the channel, photolabeling amino acids at positions M2-16 (alpha,gamm

19 n gammaM3, and to a site in the ion channel, photolabeling amino acids within each subunit M2 helix t

20 he pivotal reactive intermediate involved in photolabeling and cross-linking studies using the 8-azid

21 gA in DMPC bilayers, direct [(14)C]halothane photolabeling and microsequencing demonstrated dominant

22 or and hence should be promising ligands for photolabeling and subsequent sequencing studies.

23 oflurane binding sites were identified using photolabeling and were further validated by the docking

24 hR binding moiety, a benzophenone moiety for photolabeling, and an alkyne moiety for biotinylation vi

25 differential scanning calorimetry and lipid photolabeling, and measured the affinity of this interac

26 ,1'-binaphthyl-5,5'-disulfonic acid (BisANS) photolabeling approach to monitor changes in protein unf

27 In this study, we adapted a UV photolabeling approach, using an apolar fluorescent prob

28 nd subunit selectivity of [(3)H]azietomidate photolabeling are discussed in terms of the structures o

29 pharmacological specificity of nAChR subunit photolabeling as well as its dependence on [(3)H]tetraca

30 ids enhance rather than inhibit azietomidate photolabeling, as assayed at the level of GABA(A)R subun

31 A photolabeling assay showed that this annexin could bind

32 Propofol enhanced [(3)H]AziPm photolabeling at alphaM2-10'.

33 The photolabeling at the cytoplasmic end of the channel is f

34 fen, whereas neither drug inhibits [(3)H]CPZ photolabeling at the extracellular end, establishing tha

35 Although the efficiency of photolabeling at the subunit level was unaffected by ago

36 de, but not an adenine nucleotide, following photolabeling, but prior to cross-linking.

37 acetyl-geranylgeranyl cysteine enhanced E193 photolabeling by 3-azibutanol.

38 how reserpine- and tetrabenazine-protectable photolabeling by [125I]IAmF.

39 mer-causing mutation in PS1 strongly reduced photolabeling by a transition-state analogue but not by

40 ncentration dependence of inhibition of that photolabeling by etomidate or R-mTFD-MPAB also establish

41 tates by using electrophysiology-coordinated photolabeling by several lipophilic probes followed by m

42 Photolabeling, by exciting the fluorescent drug-tubulin

43 These photolabeling data suggest that an accessory component w

44 (3)) resulted in complete protection against photolabeling, demonstrating that [(32)P]pApAp(8-azidoA)

45 had normal ATPase activity, indicating that photolabeling did not significantly alter the enzymatic

46 mately 0.5 mol of (14)C/mol of subunit, with photolabeling distributed within the nAChR extracellular

47 l-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants o

48 ion kinetics, photoinactivation studies, and photolabeling experiments are also included; these exper

49 Photolabeling experiments have been particularly informa

50 Competitive radioligand binding and photolabeling experiments using well-characterized nonco

51 Photolabeling experiments with 8-azido-ATP demonstrate a

52 Photolabeling experiments with the McbA propeptide now i

53 Inhibition kinetics, photolabeling experiments, as well as X-ray crystallogra

54 roteins, including BamA and LptD as shown by photolabeling experiments.

55 he lipid carbonyl carbons, in agreement with photolabeling experiments.

56 o the fusion proteins were obtained from the photolabeling experiments.

57 (d) approximately 10 nM) in both binding and photolabeling experiments.

58 rescent groups for the purpose of performing photolabeling have been prepared and evaluated using the

59 IC50 = 40 mum) than it inhibited ion channel photolabeling (IC50 = 125 mum).

60 orms of RNase L has been completed utilizing photolabeling/immunoprecipitation and affinity assays, r

61 Intensity of photolabeling in each of the fractions examined coincide

62 Comparison of nAChR photolabeling in the closed state (absence of agonist) a

63 The propofol-inhibitable [(3)H]AziPm photolabeling in the GABAAR beta3 subunit in conjunction

64 unit site in the delta subunit helix bundle, photolabeling in the nAChR desensitized state (+agonist)

65 a site within the ion channel, identified by photolabeling in the nAChR desensitized state of amino a

66 and deltaCys-236); (ii) in the ion channel, photolabeling in the nAChR resting, closed channel state

67 -55 and deltaTrp-57, as the primary sites of photolabeling in the non-alpha subunits.

68 presence of agonist and the agonist-enhanced photolabeling inhibitable by phencyclidine.

69 ne concentration establish that the observed photolabeling is at the high-affinity [(3)H]tetracaine-b

70 tubulin by subtilisin after, but not before, photolabeling is blocked by probe 1.

71 The observed pattern of photolabeling is examined in relation to the predicted o

72 [(3)H]CPZ photolabeling is not detected in the transmembrane domai

73 Photolabeling may help resolve this difficulty, and thus

74 Previous photolabeling, modeling, and functional data have identi

75 ce of 20 mM glutathione, indicating that the photolabeling observed for PGHS-1 was not due to the pre

76 n of this protein with CNBr/trypsin revealed photolabeling of a 2.9-kDa peptide.

77 imulated ABCB1 ATPase activity and inhibited photolabeling of ABCB1 with [(125)I]-iodoarylazidoprazos

78 erestingly, erlotinib slightly inhibited the photolabeling of ABCB1 with [(125)I]iodoarylazidoprazosi

79 imulated ABCG2 ATPase activity and inhibited photolabeling of ABCG2 with [(125)I]-IAAP.

80 ed as antihypertensive agents, inhibited the photolabeling of ABCG2 with [(125)I]IAAP and [(3)H]azido

81 gh concentration, but it did not inhibit the photolabeling of ABCG2 with IAAP.

82 at propofol inhibited to the same extent the photolabeling of alpha1Met-236 and betaMet-286.

83 However, it is unknown whether photolabeling of alphaE262 causes functional effects in

84 Irradiation at 254 nm resulted in photolabeling of alphaTyr(198) in agonist binding site S

85 CMPI-photolabeled nAChR subunits established photolabeling of amino acids contributing to the ACh bin

86 crosequencing, we found propofol-inhibitable photolabeling of amino acids in the beta3-alpha1 subunit

87 ing state), there was tetracaine-inhibitable photolabeling of amino acids in the ion channel at posit

88 The results of photolabeling of ArsA with the ATP analogue 8-azidoadeno

89 contrast, within the same site GABA enhances photolabeling of beta3Met-227 in betaM1 by an anesthetic

90 [(3)H]Azietomidate and [(3)H]R-mTFD-MPAB photolabeling of beta3Met-227 in betaM1 established that

91 re was also propofol-inhibitable [(3)H]AziPm photolabeling of beta3Met-227 in betaM1, the amino acid

92 [(3)H]azietomidate photolabeling of beta3Met-286 in betaM3 and alpha4Met-26

93 05 in the vestibule of the ion channel, with photolabeling of both residues enhanced in the presence

94 fic, AMP-PCP-enhanced, [(3)H]azidodantrolene photolabeling of both the RyR monomer and a 160 or 172 k

95 Here, we use [(3)H]azietomidate photolabeling of bovine brain GABA(A)Rs to determine whe

96 imulated GLUT4 translocation, as assessed by photolabeling of cell surface GLUT4 with Bio-LC-ATB-BMPA

97 ition of agonist did not enhance [(125)I]TID photolabeling of deltaIle288 within the deltaM2-M3 loop.

98 Halothane photolabeling of deltaTyr-228 provides initial evidence

99 Within M1, the level of photolabeling of deltaTyr-228 with [(14)C]halothane was

100 Photolabeling of DnaA protein occurred with membrane pro

101 [35S]GTPgammaS binding, a decrease in basal photolabeling of G-proteins with azidoanilido-[alpha-32P

102 We previously identified azietomidate photolabeling of GABA(A)R alpha1Met-236 and betaMet-286

103 (A)R-modulating neurosteroids do not inhibit photolabeling of GABA(A)R alpha1Met-236 or betaMet-286 b

104 ) (GTPgammaS) binding and GTP hydrolysis and photolabeling of Galpha, we demonstrate highly efficient

105 Importantly, photolabeling of Galpha-subunits with azidoanilido-[alph

106 GABA inhibits S-[(3)H]mTFD-MPPB photolabeling of gamma2Ser-280 (gammaM2-15') in this sit

107 gamma-alpha subunit interface, identified by photolabeling of gammaMet299 within the gammaM3 helix at

108 Furthermore, [(3)H]physostigmine photolabeling of gammaTyr-111, gammaTyr-117, deltaTyr-21

109 Calmodulin antagonists increased photolabeling of hair-bundle myosin I by nucleotides.

110 ty labeling (BEProFL) approach that utilizes photolabeling of HDAC8 with a probe containing a UV-acti

111 Photolabeling of IDH with both [32P]2N3NAD+ and [2'-32P]

112 al analyses, radioligand binding assays, and photolabeling of nAChR-rich membranes with [3H]BP to ide

113 Compound 28 inhibited the photolabeling of P-gp with [(125)I]-iodoarylazidoprazosi

114 P produced time- and concentration-dependent photolabeling of protein bands of approximately 35 and 6

115 [125I]IAmF photolabeling of recombinant VMAT2, expressed in SH-SY5Y

116 specifically inhibits [(3)H]azidodantrolene photolabeling of RyR1 and its N-terminal fragment in SR.

117 Saturation of photolabeling of the 80- and the 37-kDa RNase L with the

118 8-Azidoadenosine 5'-monophosphate photolabeling of the AMP-binding site and adenylate kina

119 Photolabeling of the beta3 subunits was stereoselective,

120 Specific photolabeling of the Ca2+ ATPase with ZTG was obtained w

121 ATPase assays and photolabeling of the gpA K497A and gpA K497D mutant term

122 s not have an effect on the Kd value; and 3) photolabeling of the protein with a cysteine residue in

123 ([(125)I]TID) to compare the state-dependent photolabeling of the Torpedo nAChR before and after puri

124 The photolabeling of these amino acids suggests that when th

125 ersubunit sites, inhibited [(3)H]S-mTFD-MPPB photolabeling of these nAChR intrasubunit binding sites.

126 The photolabeling of this 80-kDa protein was saturable with

127 We now find that photolabeling of this pocket persists during the transit

128 Photolabeling of this protein by IAC was inhibited by SK

129 Photolabeling of this region indicates that the thymine

130 ein site, and propofol inhibited [(3)H]AziPm photolabeling of this site in myelin SIRT2.

131 solated from proteolytic digests established photolabeling of two residues: one within the alphaM1 tr

132 Preferential photolabeling on Pbeta from Pgamma position 40 and on Pa

133 SP prevented photolabeling only at concentrations higher than expecte

134 To achieve photolabeling or photoligation of two substrates, one is

135 esthetic steroid alphaxalone, which enhanced photolabeling, or DS-2, a delta subunit-selective positi

136 ytoplasmic end of the M2 ion channel domain, photolabeling positions M2-2, M2-6, and/or M2-9 in each

137 coupled to an alkyne-containing neurosteroid photolabeling reagent and used to identify peptide-stero

138 inding sites directly, a neurosteroid-analog photolabeling reagent, (3alpha,5beta)-6-azi-pregnanolone

139 4 was found to be an exceptionally efficient photolabeling reagent, incorporating into both alpha1 an

140 ltaM2-13') that line the channel lumen (with photolabeling reduced by >90% in the desensitized state)

141 Intramembranous photolabeling shows that (i) protonation of TNF promotes

142 the feasibility of identifying neurosteroid photolabeling sites by using mass spectrometry.

143 Lastly, the photoprobe was also effective at photolabeling Streptococcus pyogenes hyaluronate synthas

144 19-[3H]BPC-discodermolide), was selected for photolabeling studies because it had the highest extent

145 Photolabeling studies established that S-mTFD-MPPB binds

146 This was supported by photolabeling studies showing concentration- and UV-depe

147 Photolabeling studies using [3H]-3-azioctanol in Torpedo

148 Previous photolabeling studies with [(3)H]flunitrazepam identifie

149 DsRed-derived variants which we showcase in photolabeling studies, and discuss these data in terms o

150 Upon the basis of these photolabeling studies, we conclude that (1) subunits VII

151 he binding of ginkgolides to PAF receptor by photolabeling studies.

152 vative, [3H]RU58487, was used in binding and photolabeling studies.

153 rophenyl azido (pfpa) CQ analogues for PfCRT photolabeling studies.

154 In this study, we developed an intact cell photolabeling technique that allows the direct visualiza

155 (D-mannose-4-yloxy)-2-propylamine exofacial photolabeling technique, was reduced by approximately 70

156 s(D-mannos-4-yloxy)-2-propyl amine exofacial photolabeling technique.

157 The photolabeling technology developed here offers a new way

158 Torpedo californica nAChRs and time-resolved photolabeling to identify the nAChR binding sites occupi

159 candidate targets, we used a combination of photolabeling, two-dimensional gel electrophoresis, and

160 xing unit, a novel freeze-quench unit, and a photolabeling unit.

161 This was confirmed by hydrophobic photolabeling using liposomes containing trace amounts o

162 on the full ectodomain LFA-1 were probed by photolabeling using photoactivatable isoflurane (azi-iso

163 purified recombinant PfCRT, we analyze AzBCQ photolabeling versus competition with CQ and other drugs

164 gh photoinsertion yield (approximately 30%); photolabeling was abolished in the presence of excess un

165 Within alpha subunit, >/=95% of specific photolabeling was contained within a 20-kilodalton prote

166 Photolabeling was inhibited by anesthetic concentrations

167 [(32)P-5N(3)]NAADP photolabeling was irreversible in a high K(+) buffer, a

168 Predominant hydrophobic photolabeling was localized within a single region of Dn

169 in-stimulated increase in cell-surface GLUT4 photolabeling was nearly identical (approximately 3-fold

170 This pattern of photolabeling was not affected by the presence of 20 mM

171 Also, photolabeling was observed with [32P]8N3ATP and [32P]2N3

172 Photolabeling was performed after preincubation times of

173 e antagonist, or isoflurane, state-dependent photolabeling was seen in a delta subunit fragment begin

174 inhibitory effects elicited by these MSAs on photolabeling were distinct for beta-tubulin from differ

175 Photolabeling with 3-(trifluoromethyl)-3-(m-[125I]iodoph

176 vesicles of defined composition and by using photolabeling with 3-trifluoromethyl-3-(m-[125I]iodophen

177 Conversely, AMP enhanced photolabeling with 8-N3-ATP at ATP-binding site 2.

178 shows greatly decreased activity, abolished photolabeling with [32P]8N3ATP, and no detectable autoin

179 e recruitment of GLUT4 assessed by exofacial photolabeling with [3H]-ATB bis-mannose was reduced by 5

180 For both mutant and wild-type proteins, photolabeling with [3H]azidopine and [125I]iodoarylazido

181 Photolabeling with [3H]RU58487 under these optimal bindi

182 Thus, the photolabeling with [beta-32P]5-azido-UDP-GlcA has identi

183 lipid binding site, we have used hydrophobic photolabeling with a photoactivatable phosphatidylcholin

184 between Ca2+ and Na+,K+-ATPases, as well as photolabeling with a TG azido derivative, suggest that t

185 ytochrome c oxidase (CcO) were identified by photolabeling with arylazido-cardiolipin analogues and d

186 ults reported in this study demonstrate that photolabeling with azidonucleotides can be used to ident

187 rs that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in

188 ain of the Torpedo nAChR using time-resolved photolabeling with the hydrophobic probe 3-(trifluoromet

189 and cell surface GLUT4 levels as assessed by photolabeling with the membrane-impermeant reagent 2-N-(

190 Propofol inhibited [(3)H]AziPm photolabeling within the delta subunit helix bundle at l

191 lower-efficiency, state-dependent [(3)H]CMPI photolabeling within the ion channel.

192 or the purified nAChR, the agonist-sensitive photolabeling within the M2 ion channel domain of positi