ライフサイエンスコーパス: phragmoplast

1 the center of the cell in lieu of a midbody/phragmoplast.

2 e transport of Golgi-derived vesicles in the phragmoplast.

3 al signal could be detected elsewhere in the phragmoplast.

4 terials around the circumferentially growing phragmoplast.

5 reorganization and vesicle transport in the phragmoplast.

6 although some mitochondria can approach the phragmoplast.

7 reprophase band, the mitotic spindle and the phragmoplast.

8 to the midzone of the mitotic apparatus and phragmoplast.

9 rmation of a cell plate in the center of the phragmoplast.

10 nd during cytokinesis is associated with the phragmoplast.

11 trated on MTs in the spindle midzone and the phragmoplast.

12 ow that TIO is required for expansion of the phragmoplast.

13 of gamma-tubulin in the mitotic spindle and phragmoplast.

14 tiparallel MTs toward their plus ends in the phragmoplast.

15 ntiparallel MTs to be closely engaged in the phragmoplast.

16 lus ends of antiparallel microtubules in the phragmoplast.

17 t cells, cytokinesis is brought about by the phragmoplast.

18 lel microtubules in the middle region of the phragmoplast.

19 ials and their CPAMs gives rise to the solid phragmoplast.

20 ed localization in the midzone of developing phragmoplast.

21 N cell plate that is formed within the solid phragmoplast.

22 , transitional phragmoplast, and ring-shaped phragmoplast.

23 e preprophase band, mitotic spindle, and the phragmoplast.

24 and regions rich in growing plus-ends within phragmoplasts.

25 Plant cells divide using the phragmoplast, a microtubule-based structure that directs

26 Growth of phragmoplast across the cell creates a new partition in

27 LEN1 may be required for the localization of phragmoplast activity.

28 New mini-phragmoplasts also are generated de novo around the marg

29 to serious defects in MT organization in the phragmoplast and cause failures in cytokinesis.

30 gaging and bundling anti-parallel MTs in the phragmoplast and disclosed a novel action of MAP65-4 at

31 ng that short-range interactions between the phragmoplast and plasma membrane may play important role

32 t that microtubules initiate randomly in the phragmoplast and that the majority exhibit dynamic insta

33 A similar polarity developed in phragmoplasts and cell plates, raising the possibility t

34 Lack of TAN1 leads to misguided phragmoplasts and mispositioned cell walls in maize.

35 3 was localized along MTs in the spindle and phragmoplast, and its signal was pronounced in anaphase

36 within the cortical array, preprophase band, phragmoplast, and mitotic spindle.

37 t initials, solid phragmoplast, transitional phragmoplast, and ring-shaped phragmoplast.

38 TEN, formin also localized to the cell apex, phragmoplast, and to the cell cortex as dynamic cortical

39 imaging reveals that the mitotic spindle and phragmoplast are laterally displaced, and that the growi

40 The major components of the phragmoplast are microtubules, which are arranged in two

41 riety of observations suggest that expanding phragmoplasts are actively guided to the former PPB site

42 Phragmoplasts are microtubule-rich arrays that orchestra

43 Phragmoplasts are often described as initiating at the c

44 ymmetric preprophase bands prior to mitosis; phragmoplasts are subsequently guided to these asymmetri

45 Phragmoplast assembly thus provides a regular lattice of

46 dentified AtPAKRP2 (for Arabidopsis thaliana phragmoplast-associated kinesin-related protein 2).

47 phragmoplast-specific motors Kinesin-12 and Phragmoplast-Associated Kinesin-Related Protein2 to inte

48 We identify the phragmoplast-associated kinesins, PAKRP1/Kinesin-12A and

49 clusive model where microtubule dynamics and phragmoplast asymmetry are consistent with the localizat

50 s but is not essential at the midline of the phragmoplast at later stages.

51 ated specifically with bundles of MTs in the phragmoplast at or near their plus ends.

52 nto phragmoplast-like structures termed mini-phragmoplasts between both sister and nonsister nuclei.

53 Both clearly stain the spindle and the phragmoplast, but in a proportion of cells anti-DcKRP120

54 ssemble and then reform in a peripheral ring phragmoplast configuration.

55 Mini-phragmoplasts consisting of microtubule clusters assembl

56 In conclusion, we suggest that the phragmoplast contains IMTs and highly dynamic noninterdi

57 The phragmoplast coordinates cytokinesis in plants [1].

58 formed cross walls at the stage in which the phragmoplast cytoskeleton has depolymerized and the new

59 then remained near the spindle midzone until phragmoplast development, at which time they were again

60 bule and actin filament structure called the phragmoplast directs vesicles to create the new cell wal

61 aratus-derived vesicles in the center of the phragmoplast during cytokinesis in plant cells.

62 of cell division depends on guidance of the phragmoplast during cytokinesis to a cortical site marke

63 one spindle during anaphase, and finally the phragmoplast during late anaphase and telophase.

64 endent process necessary for the guidance of phragmoplasts during cytokinesis in asymmetrically divid

65 The phragmoplast executes cytokinesis in higher plants.

66 rotubules (MTs) in the cytokinetic apparatus phragmoplast exhibit an antiparallel array and transport

67 ation of a Fused kinase signalling module in phragmoplast expansion that depends upon conserved struc

68 together with actin plays a role in guiding phragmoplast expansion to the cortical division site.

69 provides a molecular mechanism for steering phragmoplast expansion.

70 keletal arrays, the preprophase band and the phragmoplast, facilitate the positioning and de novo ass

71 okinesis, the microtubule- and F-actin-based phragmoplast facilitates construction of a new cell wall

72 t on anaphase transition but causes aberrant phragmoplast formation and delays the completion of cyto

73 The mini-phragmoplasts formed between a given pair of nuclei prod

74 The phragmoplast forms at the cell center and expands to rea

75 Following nuclear division, a cytokinetic phragmoplast forms between the daughter nuclei and expan

76 Failure in phragmoplast function results in aborted or incomplete c

77 hase band formation did not prevent accurate phragmoplast fusion, and subsequent cell plate formation

78 cover new functions for TAN1 and AIR9 during phragmoplast guidance and root morphogenesis.

79 The phragmoplast has a dynamic microtubule array of two mirr

80 example the FtsZ ring in bacteria [15], the phragmoplast in plants [16], and the actomyosin ring in

81 In mutant cells, phragmoplasts in asymmetrically dividing cells are struc

82 centrosome, organization of the spindles and phragmoplasts in mitosis is known to involve the evoluti

83 the same time, the lateral expansion of the phragmoplast initials and their CPAMs gives rise to the

84 (ER) membranes are seen associated with the phragmoplast initials and with the TVN cell plate that i

85 Phragmoplast initials arise from clusters of polar micro

86 his process can be divided into four phases: phragmoplast initials, solid phragmoplast, transitional

87 tbooks is of a symmetrical process, with the phragmoplast initiating in the center of the cell and gr

88 out this process, the advancing front of the phragmoplast is in intimate contact with the parental wa

89 errations in the spatial organization of the phragmoplast-like radial microtubule arrays (RMAs) at th

90 roximately 10 microtubules in each set) into phragmoplast-like structures termed mini-phragmoplasts b

91 inases indicated as ARABIDOPSIS NUCLEUS- AND PHRAGMOPLAST-LOCALIZED KINASE1-RELATED PROTEIN KINASE1 (

92 Plant cells assemble the bipolar spindle and phragmoplast microtubule (MT) arrays in the absence of t

93 It is not well understood how the phragmoplast microtubule array is organized.

94 Once the phragmoplast microtubule array was established, AtPAKRP1

95 the establishment and/or maintenance of the phragmoplast microtubule array.

96 are incorporated into cortical, spindle and phragmoplast microtubule arrays indicating that they are

97 During cytokinesis, it localized on the phragmoplast microtubules and on the cell plate.

98 that myosin VIII associates with the ends of phragmoplast microtubules and together with actin plays

99 play a critical role in the organization of phragmoplast microtubules during cytokinesis in the micr

100 ta lead to a model whereby myosin VIII links phragmoplast microtubules to the cortical division site

101 beling experiments demonstrated that, unlike phragmoplast microtubules which are concentrated on the

102 in interphase, preprophase band, spindle and phragmoplast microtubules.

103 cells anti-DcKRP120-2 strongly decorates the phragmoplast mid-line where the plus ends of the microtu

104 -3, however, ectopic MAP65-1 appeared in the phragmoplast midline.

105 he middle, MAP65-3 exclusively decorated the phragmoplast midline.

106 gged End Binding1c (EB1c), took place in the phragmoplast midline.

107 d variable spindle orientations and enhanced phragmoplast mobility, suggesting that the PPB is involv

108 When the phragmoplast MT array appeared in a mirror pair, AtPAKRP

109 ge at unified spindle poles, and the bipolar phragmoplast MT array frequently had discrete bundles wi

110 role in establishing and/or maintaining the phragmoplast MT array, and AtPAKRP2 may contribute to th

111 nesin-12 did during the establishment of the phragmoplast MT array.

112 augmin and gamma-tubulin on the spindle and phragmoplast MT arrays and leads to serious distortions

113 is associated with acentrosomal spindle and phragmoplast MT arrays in patterns indistinguishable fro

114 following the formation of elongated bipolar phragmoplast MT arrays in the mutant.

115 tion, defective cells exhibited disorganized phragmoplast MT arrays, which caused aborted cytokinesis

116 lope breakdown, they localize on spindle and phragmoplast MTs and on the reforming nuclear envelope o

117 scopy, we observed that certain antiparallel phragmoplast MTs overlapped and were bridged by electron

118 Virtually all phragmoplast MTs terminate inside the CPAM.

119 MTs) preferentially toward spindle poles and phragmoplast MTs toward their minus ends.

120 ound to be essential for the organization of phragmoplast MTs.

121 transport along microtubules of the bipolar phragmoplast network that guides plate assembly [7-9].

122 imal cells is functionally equivalent to the phragmoplast of plants and acts to target secretion to t

123 along regions of microtubule overlap in the phragmoplast of the moss Physcomitrella patens.

124 In addition, the mutant phragmoplasts often had disorganized MT bundles with une

125 genes in Arabidopsis thaliana (A. thaliana): PHRAGMOPLAST ORIENTING KINESIN 1 and 2 (POK1, POK2).

126 The pair of kinesin-12 class proteins PHRAGMOPLAST ORIENTING KINESIN1 (POK1) and POK2 are key

127 he domain of overlapping microtubules at the phragmoplast perimeter, limiting the site of material de

128 coherent wide tubular network with the mini-phragmoplasts positioned along the network margins.

129 These mini-phragmoplasts produce a novel kind of cell plate, the sy

130 rst phase is confined to the cylinder of the phragmoplast proper and is followed by a second phase th

131 The mechanism responsible for guiding the phragmoplast remains largely unknown.

132 ion, rapid microtubule reorganization in the phragmoplast requires the orchestrated activities of mic

133 icle trafficking, they were required for the phragmoplast-specific motors Kinesin-12 and Phragmoplast

134 During the transitional phragmoplast stage, the CPAM and MTs disassemble and the

135 continues to label the midline of the early phragmoplast, suggesting a structural continuity with th

136 PB is disassembled and directs the expanding phragmoplast to the former PPB site during cytokinesis.

137 AtTAN plays a role in guidance of expanding phragmoplasts to the former PPB site.

138 n guidance of the cytokinetic apparatus, the phragmoplast, to a cortical "division site" established

139 a spatial reminder that actively guides the phragmoplast towards the cortical division site during c

140 to four phases: phragmoplast initials, solid phragmoplast, transitional phragmoplast, and ring-shaped

141 analyzed the behavior of microtubules in the phragmoplast using live-cell imaging coupled with mathem

142 is followed by a second phase that deposits phragmoplast vesicles in a concentric fashion, resulting

143 The phragmoplast was also stained.

144 associated with the mitotic spindle, and the phragmoplast was depleted when GCP4 was downregulated.

145 antiparallel MTs in the central spindle and phragmoplast was largely abolished in mutant cells lacki

146 poral and spatial organization of PDL in the phragmoplast, we termed this protein 'phragmoplastin'.

147 s, many Golgi stacks redistribute around the phragmoplast where the cell plate is formed.

148 Plant cytokinesis is brought about by the phragmoplast, which contains an antiparallel microtubule

149 nd (PPB) and the subsequent expansion of the phragmoplast, which deposits the new cell wall, to the c

150 depends on a cytoskeletal structure called a phragmoplast, which directs the formation of a new cell

151 lves a microtubule-containing structure, the phragmoplast, which guides the formation of new cell wal