ライフサイエンスコーパス: phytoene

1 sectors accumulate the carotenoid precursor phytoene.

2 eranylgeranyl diphosphate, zeta-carotene, or phytoene.

3 he sole product of the reaction was 15,15'-Z-phytoene.

4 t of the cyclopropylcarbinyl intermediate to phytoene.

5 in (GFP), did not photo-bleach or accumulate phytoene.

6 ed: dehydrosqualene (DSQ), a C30 analogue of phytoene; 10(S)-hydroxysqualene (HSQ), a hydroxy analogu

7 and responsible for the synthesis of 15-cis-phytoene, 9,9'-di-cis-zeta-carotene, and all-trans-lycop

8 Other phytoene-accumulating mutant endosperms, vp2 and white3

9 stigate this regulation further, we examined phytoene-accumulating tissue in Arabidopsis thaliana (L.

10 Previous analyses of phytoene-accumulating tissue suggested that there may be

11 Two types of phytoene-accumulating tissue were studied: Norflurazon-b

12 restored to a level sufficient for producing phytoene and downstream carotenoids.

13 Phytoene and phytofluene are major abundant dietary caro

14 Phytoene and phytofluene are precursor molecules of high

15 ) treatment resulted in high accumulation of phytoene and phytofluene in both oranges, and the biosyn

16 The bioaccessibility of phytoene and phytofluene in tomato, carrot, blood orange

17 decreased with increased heating times while phytoene and phytofluene were unchanged.

18 The bioaccessibility of phytoene and phytofluene, and also total carotenoid bioa

19 products, on carotenoid contents, including phytoene and phytofluene, as well as vitamin E.

20 The pds(-) and zds(-) strains synthesize phytoene and zeta-carotene, respectively, both of which

21 Plastoglobuli contain beta-carotene, phytoene, and galactolipids missing in CLDs.

22 after treatment were compared for lycopene, phytoene, and phytofluene contents.

23 did not cleave geranylgeranyl diphosphate or phytoene but did cleave other linear and cyclic caroteno

24 , Mg-protoporphyrin IX chelatase (bchD), and phytoene dehydrogenase (crtI) demonstrate RegA is respon

25 of the operon and the carC gene that encodes phytoene dehydrogenase.

26 hange the product of Rhodobacter sphaeroides phytoene desaturase (crtI gene product), a neurosporene-

27 Replacing the native 3-step phytoene desaturase (CrtI) with the 4-step enzyme from E

28 identified: phytoene synthase (crtB/CT1386), phytoene desaturase (crtP/CT0807), zeta-carotene desatur

29 chocystis sp. PCC 6803 the genes that encode phytoene desaturase (encoded by crtP (pds)) and zeta-car

30 ions of this FoMV vector system, four genes, phytoene desaturase (functions in carotenoid biosynthesi

31 ces is effective at silencing the endogenous phytoene desaturase (PapsPDS) gene in Papaver somniferum

32 mate-1-semialdehyde aminotransferase (GSAT), phytoene desaturase (PDS) and light-harvesting polypepti

33 Infection with TRV containing a phytoene desaturase (PDS) fragment resulted in reduced a

34 We have successfully silenced the phytoene desaturase (PDS) gene in the diploid wild speci

35 gineered with magnesium chelatase (ChlH) and phytoene desaturase (PDS) gene sequences from Nicotiana

36 cylic acid differed statistically in normal, phytoene desaturase (PDS) gene silent and diseased (infe

37 ent protein (gfp) transgene or an endogenous phytoene desaturase (pds) gene, generated a stronger and

38 ences from the RNA leader of the Arabidopsis phytoene desaturase (pds) gene, when inserted into the 3

39 ne desaturase gene (zds) or both the zds and phytoene desaturase (pds) genes of Synechocystis sp. PCC

40 Phytoene desaturase (PDS) is required for synthesizing c

41 tenoid biosynthetic pathway, a cDNA encoding phytoene desaturase (PDS) was isolated and characterized

42 h we successfully silenced the expression of phytoene desaturase (PDS), a 20S proteasome subunit (PB7

43 to zeta-carotene, carried out by the enzyme phytoene desaturase (PDS), is one of the earliest steps

44 by phytoene synthase (PSY), two desaturases (phytoene desaturase [PDS] and zeta-carotene desaturase [

45 lack colored carotenoids due to a defect in phytoene desaturase activity.

46 stewartii, and the two carotene desaturases phytoene desaturase and carotene zeta-carotene desaturas

47 ded a vector, BMVCP5, that better maintained phytoene desaturase and heat shock protein70-1 (HSP70-1)

48 arrying geranylgeranyl diphosphate synthase, phytoene desaturase and the bacterial carotene desaturas

49 to target an endogenous transcript encoding PHYTOENE DESATURASE and used to analyze the role of miR1

50 ownstream enzymes, required two desaturases (phytoene desaturase and zeta-carotene desaturase [ZDS])

51 As the phytoene desaturase and zeta-carotene desaturase used or

52 share significant similarity and a putative phytoene desaturase domain with a recently described pla

53 tem was first optimized in studies silencing phytoene desaturase expression.

54 genes of Synechocystis sp. PCC 6803 with the phytoene desaturase gene (crtI) of Rhodobacter capsulatu

55 esium chelatase subunit I gene (ChlI) or the phytoene desaturase gene (PDS).

56 mutant capable of inducing silencing of the PHYTOENE DESATURASE gene.

57 ith a control construct or one that silences phytoene desaturase had no effect on resistance or susce

58 ude that the differences in the mechanism of phytoene desaturase inhibition play an important role in

59 hereas in the dark mainly zeta-carotene, the phytoene desaturase product, accumulates, illumination l

60 f green fluorescent protein and silencing of phytoene desaturase shows that marker gene-assisted sile

61 s, and the biosynthetic activity upstream of phytoene desaturase was similar in Newhall and Cara Cara

62 ts contained an insertion in a gene encoding phytoene desaturase, an enzyme of carotenoid biosynthesi

63 ts are rescued by inhibitors or mutations of phytoene desaturase, demonstrating that phytofluene and/

64 ,15,9'-tri-cis-zeta-carotene, the product of phytoene desaturase, to form 9,9'-di-cis-zeta-carotene,

65 l carotenoid gene (crtI) encoding the enzyme phytoene desaturase, which converts phytoene into lycope

66 ne in the sequence of reactions catalyzed by phytoene desaturase.

67 siRNAs targeting an endogenous mRNA encoding PHYTOENE DESATURASE3 was introduced into a protein-codin

68 ) A and B, as well as to bacterial and plant phytoene desaturases (PHD).

69 olutionarily preserved activity of bacterial phytoene desaturases and plant carotenoid isomerases.

70 We expressed shuffled phytoene desaturases in the context of a carotenoid bios

71 Phytoene desaturases occurring in nature convert phytoen

72 Approximately 10% of known phytoene desaturases, as in Rhodobacter, produce neurosp

73 a component of a redox chain responsible for phytoene desaturation but that a redundant electron tran

74 Both GGPP formation and phytoene desaturation were elevated in these mutants.

75 ne, IM likely serves as a redox component in phytoene desaturation.

76 CrtI, which mediates lycopene formation from phytoene, does not require light, nor is a poly-cis-lyco

77 The profile of phytoene formation during ripening was also different in

78 Phytoene formation mediated by phytoene synthase (PSY) i

79 of im accumulate the noncolored carotenoid, phytoene, IM likely serves as a redox component in phyto

80 carotenoids and carotenoid precursors beyond phytoene in dark-grown mutant cells.

81 f carotenoids in ripe fruit the formation of phytoene in vitro was detected in fruit of both mutants.

82 at in cyanobacteria and plants by converting phytoene into lycopene using two plant-like desaturases

83 e enzyme phytoene desaturase, which converts phytoene into lycopene.

84 ue to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene.

85 ical isomers of major carotenoids in humans (phytoene, phytofluene, lutein, zeaxanthin, beta-cryptoxa

86 ces were in carotenoids, including lycopene, phytoene, phytofluene, neurosporene, and zeta-carotene.

87 Phytoene, phytofluene, zeta-carotene, neurosporene, tetr

88 ed highest amounts of potentially absorbable phytoene/phytofluene was by far tomato juice (5mg/250mL

89 the following six enzymes to be identified: phytoene synthase (crtB/CT1386), phytoene desaturase (cr

90 Phytoene synthase (PSase) catalyzes the condensation of

91 ined, or sequence or abundance of mRNAs from phytoene synthase (PSY) and chromoplast-specific lycopen

92 Phytoene synthase (PSY) catalyzes the highly regulated,

93 Phytoene formation mediated by phytoene synthase (PSY) is rate limiting in the biosynth

94 Phytoene synthase (PSY) is the crucial plastidial enzyme

95 Phytoene synthase (PSY) mediates the first committed ste

96 Phytoene synthase (PSY), a major rate-controlling carote

97 limiting enzyme for carotenoid biosynthesis, phytoene synthase (PSY), as compared with white-rooted c

98 hypothesized that the daffodil gene encoding phytoene synthase (psy), one of the two genes used to de

99 ent, we began to characterize genes encoding phytoene synthase (PSY), since this nuclear-encoded enzy

100 control the expression of the gene encoding PHYTOENE SYNTHASE (PSY), the first and main rate-determi

101 Phytoene synthase (PSY), the rate-limiting enzyme in the

102 ceed through a poly-cis pathway catalyzed by phytoene synthase (PSY), two desaturases (phytoene desat

103 d levels by posttranscriptionally regulating phytoene synthase (PSY).

104 c pathway is mediated by the nuclear-encoded phytoene synthase (PSY).

105 isrupting the activity of the fruit-specific phytoene synthase (PSY1), the first committed step in th

106 hosphate (IPP) was used as the substrate for phytoene synthase a reduction (e.g. r,r mutant, 5-fold)

107 Contrastingly, reduced phytoene synthase activity was not detected when [3H]GGP

108 rotenoid content, on two maize genes, the Y1 phytoene synthase and PSY2, a putative second phytoene s

109 Upon induction, recombinant phytoene synthase constituted 5-10% of total soluble pro

110 DNA sequencing of the phytoene synthase gene from each of the mutants revealed

111 e resulting from the up-regulation of the Y1 phytoene synthase gene product in endosperm tissue.

112 Transformation with a wild-type copy of the phytoene synthase gene was able to complement the lts1-2

113 ts examined in this work are affected in the phytoene synthase gene.

114 as closely linked to a marker located in the phytoene synthase gene.

115 hese data were supported by the detection of phytoene synthase protein on western blots.

116 matoes is due to a mutated or down-regulated phytoene synthase protein, respectively, resulting in th

117 The crtB gene encoding phytoene synthase was isolated from a plasmid containing

118 d the expression of HMG2, PSY1 (the gene for phytoene synthase), and lycopene accumulation before the

119 s, as well as with the enhanced abundance of phytoene synthase, a key enzyme in the carotenoid biosyn

120 ymes geranylgeranyl diphosphate synthase and phytoene synthase, from the soil bacterium Erwinia stewa

121 The FN68 mutant is deficient in phytoene synthase, the first enzyme of the carotenoid bi

122 te synthase rather than squalene synthase or phytoene synthase, which catalyze c1'-2-3 cyclopropanati

123 es were concurrently upregulated, except for phytoene synthase, which was repressed.

124 anisms, acting predominantly at the level of phytoene synthase-1 (PSY1), and feed-forward mechanisms

125 fruit phenotype of the r,r mutant and Psy-1 (phytoene synthase-1) antisense tomatoes is due to a muta

126 s and Myxococcus xanthus crtB genes encoding phytoene synthase.

127 s of maize was sequenced and found to encode phytoene synthase.

128 hytoene synthase and PSY2, a putative second phytoene synthase.

129 n bacteria and bifunctional lycopene cyclase-phytoene synthases in fungi.

130 embrane-bound portion mediated a doubling of phytoene synthesis rates.

131 gments became photo-bleached and accumulated phytoene (the substrate for PDS) in a manner similar to

132 Moreover, phytoene, the precursor of the pathway, was identified i

133 oene desaturases occurring in nature convert phytoene to either neurosporene or lycopene in most euba

134 sport chain and convert the colorless 15-cis-phytoene to the red-colored all-trans-lycopene.

135 The two-step desaturation of phytoene to zeta-carotene, carried out by the enzyme phy

136 o catalyze two desaturation steps converting phytoene to zeta-carotene.

137 rsion of the C40 carotenoid backbone, 15-cis-phytoene, to all-trans-lycopene, the geometrical isomer

138 duced six rather than four double bonds into phytoene, to favor production of the fully conjugated ca

139 Phytoene was consistently the carotenoid with the highes

140 e.g. r,r mutant, 5-fold) in the formation of phytoene was observed with an accumulation (e.g. r,r mut

141 Plasma concentrations of phytofluene and phytoene, which were present in small amounts in tomato