ライフサイエンスコーパス: pili

1 nge electron exchange without the need for e-pili.

2 rom both the bacterial flagellum and type IV pili.

3 c bacterial pathogen that expresses type IVa pili.

4 d to vary greatly between flexible and stiff pili.

5 he absence of appendages such as flagella or pili.

6 yr3 mutant, which produces poorly conductive pili.

7 ns, suggesting that it was incorporated into pili.

8 to form filaments with dimensions similar to pili.

9 directional motive force comes from Type IV pili.

10 nding adhesin at the tip of bacterial type 1 pili.

11 lular photoreceptors and mediated by Type IV pili.

12 ce factors diphtheria toxin and the adhesive pili.

13 conformational changes in stretched type IV pili.

14 on which specifies pyelonephritis-associated pili.

15 ng Clostridium difficile, to produce Type IV pili.

16 , CdiA-CT(536) import requires conjugative F pili.

17 rt of substrates and/or extrusion of type IV pili.

18 es the presence of pilins, but not assembled pili.

19 w for infection of strains with glycosylated pili.

20 is true for certain Gram-negative bacterial pili.

21 ch were genes that specify sortase-dependent pili.

22 ith isolates that were positive for the SpaA pili.

23 , type III secreted effectors, flagella, and pili.

24 t synthesis machinery with the flagellum and pili.

25 efficient cell wall anchoring of mature Ebp pili.

26 eous for the presence of SpaD- and SpaH-type pili.

27 the bacterium to express functional type IV pili.

28 t for the order of subunits in native type 1 pili.

29 d to the extraordinary strength of bacterial pili.

30 ering insights into the structure of type IV pili.

31 eria contain a highly diverse set of type IV pili.

32 r, but required for formation of conjugative pili.

33 eased expression of mannose-sensitive type 1 pili.

34 or the antiphagocytic effect attributable to pili.

35 occi that expressed adhesive RrgA-containing pili.

36 us stiffness and the location of adhesins on pili.

37 using the mechanical activity of its type IV pili, a major surface adhesin.

38 y a positive feedback that increases type IV pili activity, thereby promoting long-term surface attac

39 ty, Actinomyces oris expresses proteinaceous pili (also called fimbriae) to mediate the following two

40 ence such as toxins, adhesins, flagella, and pili, among others.

41 s utilized by these bacteria are the type IV pili and a protein O-glycosylation system.

42 of leviviruses, allowing the toxin to bind F pili and become internalized during pilus retraction.

43 d to nutrient availability for production of pili and exopolysaccharide adhesion structures.

44 which have surfaces decorated with discrete pili and form a dispersed layer of cells on a plastic su

45 We identified EmpA as the tip of the pili and found that deletion of empA reduced biofilm for

46 pilus biosynthesis, results in cells lacking pili and having an adhesion defect, it does not affect m

47 mechanism that is distinct from other known pili and likely represents a different type of bacterial

48 ynechocystis sp. PCC 6803 moves with Type IV pili and measures light intensity and color with a range

49 s for intracellular coordination of multiple pili and of pili with other motility machines, ranging f

50 provided by re-elongation of fully retracted pili and pilus bundling.

51 d by the extension and retraction of type IV pili and requires the presence of exopolysaccharides (EP

52 us cells is mediated by motile cells bearing pili and that their contact with a surface results in th

53 This leads to the conclusion that type 4 pili and the DNA translocator are distinct systems.

54 emonstrate that G. sulfurreducens conductive pili and the outer membrane extensions of S. oneidensis

55 xococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts

56 ajectory shape and frictional forces between pili and the surface: strong pili-surface interactions g

57 NA), FasX, which regulates the expression of pili and the thrombolytic agent streptokinase.

58 PilX require PilY1 for inclusion in surface pili and vice versa, suggestive of complex formation.

59 rial type 2 secretion systems (T2SS), type 4 pili, and archaeal flagella assemble fibres from initial

60 de up to 16 distinct chaperone-usher pathway pili, and each pilus type may enable colonization of a h

61 , demonstrate its incorporation into Type IV pili, and offer insights into how the Type IV pili of C.

62 tance between the surface and polar adhesive pili, and orienting pili to face the surface.

63 n-pilin protein PilY1 for incorporation into pili, and that with FimU, PilE may couple the priming su

64 everal bioelectrochemical technologies and e-pili are a promising renewable source of electronic mate

65 observed conductive properties of Geobacter pili are a valuable tool to guide further investigation

66 Chaperone-usher pathway pili are a widespread family of extracellular, Gram-nega

67 monstrate a mechanism by which Gram-positive pili are able to dissipate mechanical energy through mec

68 nsion is a quasistatic process such that the pili are able to relax via thermal fluctuations as it is

69 Geobacter sulfurreducens pili are actual wires.

70 These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholip

71 and structural analyses reveal that F17-like pili are closely related to pilus types carried by intes

72 Pneumococcus-expressed pili are composed of three structural proteins: RrgB, wh

73 The Streptococcus pnuenomae pilus island 1 pili are composed of three subunits, RrgA, RrgB, and Rrg

74 piliated species, it is unclear how multiple pili are coordinated to generate movement with direction

75 utant cells (0.2 events/min), indicating the pili are critical structures in the transition from reve

76 llular fibers called chaperone-usher pathway pili are critical virulence factors in a wide range of G

77 hair-like cell appendages denoted as type IV pili are crucial for biofilm formation in diverse eubact

78 These observations suggest that MSHA pili are crucial for surface selection, irreversible att

79 Thus, pili are dispensable for biofilm development in this cya

80 Pili are elongated structures that protrude from bacteri

81 The pili are essential for long-range electron transport to

82 Type IV pili are extracellular polymers of the major pilin subun

83 Pili are fibrous appendages expressed on the surface of

84 Type IVa pili are filamentous cell surface structures observed in

85 We show that the rupture forces between pili are fine-tuned by post-translational modification.

86 Type IV pili are important for microcolony formation, biofilm fo

87 We and others have shown that type IV pili are important for protein secretion across the oute

88 Type IV pili are important virulence factors for many pathogens,

89 Type 1 pili are important virulence factors in uropathogenic Es

90 Type IV pili are important virulence factors on the surface of m

91 Ubiquitous microbial appendages called pili are involved in sensing surfaces and facilitating d

92 The SpaA pili are known to mediate bacterial adherence to pharyng

93 Type IV pili are long fibers that are assembled by polymerizatio

94 Type IV pili are long, protein filaments built from a repeating

95 Conversely, stiff pili are much more likely to make contact with the subst

96 ria, extracellular protein appendages termed pili are necessary for adherence under mechanical stress

97 Type IV pili are polymeric bacterial appendages that affect host

98 Type IV pili are produced by many pathogenic Gram-negative bacte

99 Pili are proteinaceous polymers of linked pilins that pr

100 Types 1 and P pili are prototypical bacterial cell-surface appendages

101 Type 1 pili are representative of a class of bacterial surface

102 nvelope protein PilY1 and functional type IV pili are required mechanosensory elements.

103 Pili are surface-attached, fibrous virulence factors tha

104 Type IV pili are ubiquitous bacterial motors that power surface

105 Conjugative pili are widespread bacterial appendages that play impor

106 The putative pili are, in fact, long extensions of the cytochrome-ric

107 in the DeltapilA[1-6]DeltaaglB cells, these pili are, unlike wild-type pili, curled, perhaps renderi

108 These "competence pili" are composed of the major pilin protein ComGC and

109 Fimbriae (also known as pili) are appendages that extend up to 2 mum beyond the

110 se proteins, and protein assemblies, such as pili, are typically long and thin yet must withstand hig

111 The mobile charge density in the pili, as well as the temperature and pH dependence of th

112 These pili, assembled by the chaperone-usher pathway, are poly

113 f the ctpABCDEFGHI genes (cluster of type IV pili; Atu0216 to Atu0224), homologous to tad-type pilus

114 iety of virulence factors, including type IV pili, bacterial extracellular appendages often essential

115 ficiently move toward chemoattractants using pili-based "twitching" motility and the Chp chemosensory

116 ructural biology, that meningococcal type IV pili bind DNA through the minor pilin ComP via an electr

117 plastic surface, DeltaaglB cells have thick pili bundles and form microcolonies.

118 hat do not depend on traditional flagella or pili, but are powered by mechanisms that are less well u

119 ling a new pilin superfamily, assembled into pili by a distinct fifth pathway.

120 he high force extensibility, CnaA-containing pili can dissipate approximately 28-fold as much energy

121 starting point for understanding how type IV pili can mediate secretion of virulence factors importan

122 Electrically conductive pili (e-pili) can be an important electrical conduit for DIET.

123 m-the usher-is critical for the formation of pili, catalysing the polymerisation of pilus subunits an

124 red for assembly of their respective Type IV pili, CFA/III and Longus.

125 aglB cells, these pili are, unlike wild-type pili, curled, perhaps rendering them non-functional.

126 tive pili remains uncertain, largely because pili-defective mutants also have cytochrome defects.

127 Here we report that a pili-deficient mutant carrying an inactivating mutation

128 contact with a surface results in the rapid pili-dependent arrest of flagellum rotation and concurre

129 faces as structured swarms utilizing type IV pili-dependent social (S) motility.

130 h respect to the presence of surface-exposed pili, DNA uptake, and natural transformability.

131 It is shown that very flexible pili do not extend very far and thus would limit the bac

132 us-minus mutant cells (13 s), suggesting the pili do not play a significant role in reversible adhesi

133 A number of the mutants did not exhibit any pili during growth at 64 degrees C but still were transf

134 Electrically conductive pili (e-pili) can be an important electrical conduit for

135 the major component of Enterococcus faecalis pili, EbpC, labels polymerized pilus structures, diminis

136 bacteria to short jumps forward while stiff pili enable much greater displacements.

137 g SpaA-type pili or devoid of toxin and SpaA pili exhibited delayed killing of nematodes with similar

138 odel in which the incorporation of PilJ into pili exposes the C-terminal domain of PilJ to create a n

139 The pili extend, attach to the surface, and then retract to

140 In many bacteria and archaea, type IV pili facilitate surface adhesion, the initial step in bi

141 t robustness to incorporate further advanced pili features and various cell geometries to describe ot

142 , DNABII (DNA binding and bending) proteins, pili, flagella, and outer membrane vesicles.

143 d by surface structures such as flagella and pili, followed by a permanent adhesion stage usually med

144 tic Pf4 bacteriophage, which may use type IV pili for infection.

145 ate that P. aeruginosa not only uses type IV pili for surface-specific twitching motility, but also a

146 e product in protein secretion as well as in pili formation.

147 ross-links, formed autocatalytically, in the pili from Streptococcus pyogenes has highlighted the rol

148 e pneumococcus, the coordinated secretion of pili from the cells correlates to DNA transformation.

149 Individual pili function independently but can lead to coordinated

150 odels of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstruct

151 cal conductivity of Geobacter sulfurreducens pili has been documented with multiple lines of experime

152 Pili have been shown to play a role in the pathogenesis

153 hewanella oneidensis also produce conductive pili have recently been recanted, based on novel live-im

154 Filamentous structures termed "pili" have been visualized at the interface between H. p

155 stricted in the bacterial cell (e.g. type IV pili, holdfasts, chemoreceptors), but perhaps none show

156 Physically blocking pili imposed resistance to pilus retraction, which was s

157 on the remarkable properties of conjugative pili in bacterial secretion and phage infection.

158 show that PilJ is incorporated into Type IV pili in C. difficile and present a model in which the in

159 This work signifies the important role of pili in corynebacterial pathogenesis and provides a simp

160 olved in the formation of competence-induced pili in Gram-positive bacteria and corroborate the remar

161 uggesting a role for the proper anchoring of pili in retaining OM integrity.

162 equired for pilus formation, and the role of pili in T4SS function is unclear.

163 in trans is sufficient to produce functional pili in the DeltapilA[1-6] strain.

164 Although PilA4 assembles into pili in the DeltapilA[1-6]DeltaaglB cells, these pili ar

165 roteins enabling assembly and secretion of P pili in uropathogenic E. coli.

166 e is known about the expression of different pili in various clinical isolates and their importance i

167 cts with human endothelial cells via type IV pili in vitro.

168 ently confirmed charge propagation along the pili, in a manner similar to carbon nanotubes.

169 ectin located at the tip of bacterial type 1 pili, interacts with mannosylated glycoproteins on the u

170 of the structurally related archaeal type IV pili is unknown.

171 pable of long-range electron transport along pili, known as microbial nanowires, that have metallic-l

172 ptidase, but it does not assemble functional pili, leading us to conclude that Duf1628 can be annotat

173 ew densely packed ribosomes and a variety of pili-like structures that might enable inter-organism in

174 a, and twitching motility powered by Type IV pili, little is known about gliding motility.

175 ogarithmic range of detection (i.e., 3-7 for pili-mannose binding and 2-8 for Con A mediated binding)

176 ce (QCM) transducers and by using the direct pili-mannose binding as well as Concanavalin A (Con A) m

177 stream behaviors, but the mechanism by which pili mediate surface sensing has been unclear.

178 In Gram-positive bacteria, sortase-dependent pili mediate the adhesion of bacteria to host epithelial

179 Like other chemotaxis pathways, pili-mediated surface sensing results in a transient res

180 channel, in contrast to previously existing pili models.

181 that regulates hair growth and the arrector pili muscle, which controls piloerection.

182 iated structures: sebaceous glands, arrector pili muscles, Merkel cells, and sensory nerve endings.

183 ay but not the functioning of the conductive pili network.

184 Pili networks confer conductivity to G. sulfurreducens b

185 sting that bifidobacterial sortase-dependent pili not only contribute to adherence but also display i

186 ellular milieu (exo-proteome) and eliminated pili observable by electron microscopy.

187 ili, and offer insights into how the Type IV pili of C. difficile may assemble and function.

188 g key aromatic amino acids, suggest that the pili of G. sulfurreducens function as molecular wires wi

189 tite attached to the electrically conductive pili of Geobacter species in a manner reminiscent of the

190 directly visualize charge propagation along pili of Geobacter sulfurreducens with nanometre resoluti

191 e multi-heme c-type cytochrome OmcS with the pili of Geobacter sulfurreducens.

192 pproach to test the hypothesis that multiple pili of Neisseria gonorrhoeae are coordinated through a

193 to the cell surface allows for production of pili of sufficient length to support adherence and motil

194 ereby compensating for the reduced number of pili of the N3 mutant.

195 PilA1 and PilA2, the most abundant pilins in pili of wild-type and DeltaaglB strains, are modified un

196 a plethora of colonization factors (fimbriae/pili), of which CFA/I and CFA/II, which are assembled vi

197 s provided new insights into the assembly of pili on the surface of bacteria.

198 Bacillus cereus strains elaborate pili on their surface using a mechanism of sortase-media

199 data, we propose a model for the collective pili operation of N. gonorrheae bacteria that explains t

200 genic mutants of NCTC13129 lacking SpaA-type pili or devoid of toxin and SpaA pili exhibited delayed

201 uce protein polymers on their surface called pili or fimbriae that serve either as attachment devices

202 johnsoniae, a rod-shaped bacterium devoid of pili or flagella, glide over glass at speeds of 2-4 mum/

203 formation occurs in the absence of flagella, pili, or certain polysaccharides.

204 eriously on surfaces without using flagella, pili, or other external appendages.

205 ults support a model in which the conductive pili permeate the biofilms to wire the cells to the cond

206 Bacterial surface fibers (pili) permit adherence to biotic and abiotic substrates,

207 pneumoniae expresses two different types of pili, PI-1 and PI-2, both of which require the concerted

208 Cell surface appendages called pili play an important role in adhesion and biofilm form

209 Type IV pili play important roles in a wide array of processes,

210 Pili, polymerized protein structures covalently anchored

211 Type 1 pili, produced by uropathogenic Escherichia coli, are mu

212 their biofilm-promoting function in type IV pili-producing heterotrophic bacteria.

213 tween cyanobacteria and well-studied type IV pili-producing heterotrophic bacteria.

214 ouse model, we show that F17-like and type 1 pili promote intestinal colonization and show distinct b

215 er region at sites different from the type 1 pili promoter and independent of integration host factor

216 ting experiments revealed that expression of pili proteins does not differ in geographically differen

217 t are functional components of flagellin and pili proteins within clinically relevant Gram-negative b

218 long-range charge transport along individual pili purified free of metal and redox organic cofactors

219 Genes encoding types I and IV pili, quorum sensing components, and proteins involved i

220 the contribution of the biofilm's conductive pili remains uncertain, largely because pili-defective m

221 Nevertheless, pili removal is not required for biofilm formation as ev

222 f pilR2 resulted in a reduction of assembled pili, significant decreases in EPS production and loss o

223 We show that by changing pili substrate interactions, the motility pattern can be

224 forces between pili and the surface: strong pili-surface interactions generate orbiting motion, incr

225 However, the mechanism by which the pili surrounding the cell body work together to propel b

226 nnose-sensitive hemagglutinin (MSHA) type IV pili synergistically to switch between two complementary

227 tes key virulence characteristics, including pili synthesis, biofilms, and motility, resulting in vir

228 lfolobus cells and act downstream of the Ups pili system.

229 Type 1 pili (T1P) are major virulence factors for uropathogenic

230 n features, T4P are classified into type IVa pili (T4aP) and type IVb pili (T4bP)(1,2).

231 cluding Pseudomonas aeruginosa, use type IVa pili (T4aP) for attachment and twitching motility.

232 ified into type IVa pili (T4aP) and type IVb pili (T4bP)(1,2).

233 ndent on extension and retraction of Type-IV pili (T4P) and production of extracellular polysaccharid

234 stems and are linked to extrusion of type IV pili (T4P) and to DNA uptake.

235 Type IV pili (T4P) are among the key virulence factors used by P

236 Type IV pili (T4P) are filamentous appendages found on many Bact

237 Type IV pili (T4P) are ubiquitous and versatile bacterial cell s

238 Type IV pili (T4P) are ubiquitous bacterial cell surface structu

239 Type IV pili (T4P) are very thin protein filaments that extend f

240 on systems that regulate motility by type IV pili (T4P) can be markedly more complex than related fla

241 Type IV pili (T4P) contain hundreds of major subunits, but minor

242 Neisseria meningitidis use Type IV pili (T4P) to adhere to endothelial cells and breach the

243 a exhibit surface motility powered by type 4 pili (T4P).

244 ransporter adhesins, O antigens, and type IV pili (T4P).

245 xins, or filamentous phages; extrude type IV pili (T4P); or take up DNA.

246 and appendages known as flagella and type IV pili (TFP) are known to confer such motility.

247 Type IV pili (TFP) function as mechanosensors to trigger acute v

248 In Gram-negative bacteria, type IV pili (TFP) have long been known to play important roles

249 ough the extension and retraction of type IV pili (TFP) on solid surfaces, which requires both TFP an

250 orm of PilA [the majority subunit of Type IV pili (Tfp) produced by NTHI], mediated gradual 'top-down

251 ")-motility mechanism is mediated by type IV pili (TFP), linear actuator appendages that propel the b

252 ve across surfaces by using multiple Type IV Pili (TFP), motorized appendages capable of force genera

253 Type IV pili (Tfp), which are key virulence factors in many bact

254 Type IV pili (Tfp), which have been studied extensively in a few

255 emerged as a model for the study of type IV pili (Tfp)-exceptionally widespread and important prokar

256 man pathogen Streptococcus pyogenes produces pili that are essential for adhesion to host surface rec

257 13129 produces three distinct heterotrimeric pili that contain SpaA, SpaD, and SpaH, making up the sh

258 dermal collagen, sweat glands, and arrector pili that engulfed axons.

259 While it is not always the tip of flexible pili that first makes contact with the substrate, it is

260 y observed metallic like conductivity of the pili that has been attributed to overlapping pi-pi orbit

261 substrate, it is likely to be a part of the pili that is close to the tip.

262 the resistance on retracting, surface-bound pili that occurs upon surface contact.

263 e bacterial cell wall or assemble fiber-like pili that promote bacterial adhesion.

264 with a lack of charge propagation in mutated pili that were missing key aromatic amino acids, suggest

265 long and flexible filaments, called type IV pili, that extend from the cell body, attach to the surf

266 toxins, RHS proteins, adhesins, and type IV pili] that likely mediate cell-cell interactions and gut

267 (e.g., secretion systems, cellular capsule, pili), the role of the large cryptic secondary metabolom

268 Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the

269 re, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron

270 For Ebp and other sortase-assembled pili, the pilus-associated sortases are essential for fi

271 ion of the metallic-like conductivity of the pili, their role in biogeochemical cycling, and applicat

272 f movement over surfaces without flagella or pili; they glide.

273 ion mechanism requires attachment of type IV pili to a solid surface, followed by pilus retraction an

274 Thus, UPEC employ separate CUP pili to adapt to the rapidly changing niche during bladd

275 Moving cells also use pili to aggregate and form microcolonies.

276 rface and polar adhesive pili, and orienting pili to face the surface.

277 pping as the mechanism that allows Geobacter pili to function as protein nanowires between the cell a

278 counter-rotates the cell body, causing MSHA pili to have periodic mechanical contact with the surfac

279 a such as Pseudomonas aeruginosa use type IV pili to move across surfaces.

280 tries to describe other bacteria that employ pili to move on surfaces.

281 xpression of conductive protein filaments or pili to respire extracellular electron acceptors such as

282 gen Streptococcus pneumoniae deploys type IV pili to take up DNA during transformation.

283 flagellum and one or two clusters of type IV pili, to the cell poles.

284 , also known as "spun glass hair syndrome," "pili trianguli et canaliculi," or "cheveux incoiffables"

285 nal deletion exhibits reduced sensitivity to pili-tropic phage PhiCbK, resulting from reduced pilA ge

286 Superfamily ATPases in type IV pili, type 2 secretion, and archaella (formerly archaeal

287 is known about the behavior of Gram-positive pili under force.

288 We visualized Caulobacter crescentus pili undergoing dynamic cycles of extension and retracti

289 to biofilm formation (BopD), adherence (Epb pili), virulence (cps loci, gelatinase, SprE) and antibi

290 ction speeds measured at the level of single pili, we build a tug-of-war model.

291 retical energy-minimized models of Geobacter pili were constructed with a previously described approa

292 he role of a virulence factor, the SpaA-type pili were found to be prevalent among the isolates, and

293 es (likely to be outer membrane proteins and pili) which, upon contacting the membrane, undergo surfa

294 Movement depends on Type IV pili, which are extended, adhere to the substrate and th

295 UPEC express CUP pili, which are extracellular fibers tipped with adhesin

296 at a single point into individual, untreated pili, which are still attached to cells, propagated over

297 he crenarchaeal genus Sulfolobus express Ups pili, which initiate cell aggregate formation.

298 odes a novel adhesin associated with type IV pili, which was identified in the exoproteome bound to c

299 Results are generated for pili with different rigidities ranging from very flexibl

300 ellular coordination of multiple pili and of pili with other motility machines, ranging from physical