ライフサイエンスコーパス: pilus

1 gRNA and is required for adsorption to the F-pilus.

2 ely represents a different type of bacterial pilus.

3 strating how it may be incorporated into the pilus.

4 we determine the structure of the assembled pilus.

5 pilus assembly and are incorporated into the pilus.

6 e Yts2 type II secretion system, and the Tad pilus.

7 factors cholera toxin and toxin-co-regulated pilus.

8 ired for attachment of the phage to the host pilus.

9 ter in Gram-positive bacteria may hijack the pilus.

10 2 domain of the phage gene-3-protein to an F pilus.

11 translocation channel and an extracellular T pilus.

12 ructural component of the type III secretion pilus.

13 volving HrpA, the main component of the T3SS pilus.

14 the presence of an adhesive RrgA-containing pilus-1 mediate pneumococcal passage from the bloodstrea

15 w that the major adhesin of the pneumococcal pilus-1, RrgA, binds both receptors, whereas the choline

16 e determined that expression of pneumococcal pilus-1, which includes the pilus adhesin RrgA, promotes

17 de and lipopolysaccharide, toxin coregulated pilus A, sialidase, hemolysin A, flagellins (FlaB, FlaC,

18 bias likely results from the polarization of pilus activity, yielding variable levels of movement in

19 For example, the type 1 pilus adhesin FimH binds mannose on the bladder surface,

20 of pneumococcal pilus-1, which includes the pilus adhesin RrgA, promotes bacterial penetration throu

21 The pilus adhesin tip protein Cpa promoted Alab49 survival i

22 loped antivirulence inhibitors of the type 1 pilus adhesin, FimH, demonstrated oral activity in anima

23 The pKM101 OMCC cap and extended pilus also are dispensable for activation of a Pseudomon

24 responsible for attaching the virus to an F-pilus and delivering the viral genome into the host duri

25 nsight into genome delivery via the host sex pilus and mechanisms underlying ssRNA-capsid co-assembly

26 s), we sought to test whether FasX regulates pilus and streptokinase expression in a serotype-specifi

27 idual pilus subunits EmpA, EmpB, and EmpC to pilus architecture, biofilm formation, adherence to extr

28 impeding the analysis of a complete model of pilus architecture.

29 l for DNA transformation is proposed whereby pilus assembly "drills" a channel across the thick cell

30 structures provide the molecular basis for F pilus assembly and also shed light on the remarkable pro

31 nd thiol-disulfide oxidoreductase, abrogates pilus assembly and alters cell morphology.

32 n determining sortase specificity during the pilus assembly and anchoring.

33 ns, the minor pilins FimU and PilVWXE, prime pilus assembly and are incorporated into the pilus.

34 omponent of the T4P system, controlling both pilus assembly and disassembly.

35 of a differential effect of glycosylation on pilus assembly and function of paralogous pilins.

36 Minor pilins influence pilus assembly and retraction, but their precise functio

37 s indicate that the ctp locus is involved in pilus assembly and that nonpiliated mutants, which retai

38 indicating that the TG motif is critical in pilus assembly and that they govern the pilin-specific a

39 diverse cellular functions in cell division, pilus assembly and toxin production, we propose that Mdb

40 at Pseudomonas aeruginosa minor pilins prime pilus assembly and traffic the pilus-associated adhesin

41 ded mutant proteins that supported wild-type pilus assembly but had a reduced capacity to support twi

42 bon utilization, siderophore production, and pilus assembly demonstrate frequent gain or loss among i

43 the FimD usher is able to catalyse in vitro pilus assembly effectively in its monomeric form.

44 racterization of mCherry fusions to modulate pilus assembly from within the periplasm.

45 Pilus assembly in bacteria typically occurs by one of fo

46 Type IV pilus assembly involves a conserved group of proteins th

47 The protein complex responsible for type IV pilus assembly is homologous with the type II protein se

48 ant carrying an inactivating mutation in the pilus assembly motor PilB has no measurable defects in c

49 The pilus assembly system, which promotes surface-associated

50 We propose a model for CofB-initiated pilus assembly with implications for understanding filam

51 w levels of the minor pilins are optimal for pilus assembly, and CofB can be detected in the pilus fr

52 oris, disulfide bond formation is needed for pilus assembly, coaggregation, and biofilm formation.

53 aperone-usher pathway ushers, which catalyze pilus assembly, contain five functional domains: a 24-st

54 ng the C-terminal disulfide bridge abrogates pilus assembly, in turn eliminating biofilm formation an

55 and could serve as a driving force for CFA/I pilus assembly, representing an energetic landscape uniq

56 inor pilins, thereby providing insights into pilus assembly, structure, and function.

57 inal region is required for CofB to initiate pilus assembly.

58 a vitamin K epoxide reductase (VKOR) gene in pilus assembly.

59 the major pilin PilA, allowing for efficient pilus assembly.

60 d the minimal set of components required for pilus assembly.

61 Deletion of all minor subunits abrogated pilus assembly.

62 ilin subunits in the inner membrane prior to pilus assembly.

63 reased OM permeability but no loss in type 1 pilus assembly.

64 ore, which was unable to efficiently mediate pilus assembly.

65 t with a predicted salt bridge necessary for pilus assembly.

66 omain from the bifunctional and bipolar ZitP pilus assembly/motility regulator revealed conserved int

67 pilins prime pilus assembly and traffic the pilus-associated adhesin and anti-retraction protein, Pi

68 or Ebp and other sortase-assembled pili, the pilus-associated sortases are essential for fiber format

69 f bacteria with antibodies against the major pilus backbone subunit (RrgB) or the adhesin component (

70 e of the Shiga toxin genes or bundle-forming pilus (BFP) genes.

71 erence factor plasmid-encoded bundle-forming pilus (bfp) genes.

72 ements specialized developmental functions - pilus biogenesis and multifactorial swarming motility -

73 The traffic AAA-ATPase PilF is essential for pilus biogenesis and natural transformation of Thermus t

74 of cag PAI genes that are required for both pilus biogenesis and T4SS function and reveal that these

75 on the presence of components of the type IV pilus biogenesis apparatus for secretion have been repor

76 mall subset of four proteins dispensable for pilus biogenesis are essential for motility.

77 rizing TM helix from glycophorin A blocked T-pilus biogenesis in A. tumefaciens.

78 Although the mechanism of pilus biogenesis is well characterized, how the usher it

79 owever, zinc binding is neither required for pilus biogenesis nor natural transformation.

80 provided insight into the initiation step of pilus biogenesis resulting in pore activation, but very

81 t these proteins are secreted by the type IV pilus biogenesis system.

82 will focus on the chaperone-usher pathway of pilus biogenesis, a widespread assembly line for pilus p

83 se a regulatory model of the L. rhamnosus GG pilus biogenesis.

84 ue molecular mechanisms of sortase-assembled pilus biogenesis.

85 on via its Leu1 motif that is critical for T-pilus biogenesis.

86 has important implications for understanding pilus biology.

87 rium to endure the periplasmic stress of sex pilus biosynthesis during mating.

88 ral processes, including phototaxis, type IV pilus biosynthesis, photosystem II levels, biofilm forma

89 e deleting pilB3-C3, genes required for PilA pilus biosynthesis, results in cells lacking pili and ha

90 These observations, obtained with a simple pilus biosynthetic system, are likely to be applicable t

91 by re-elongation of fully retracted pili and pilus bundling.

92 enic Escherichia coli produces a long type 4 pilus called Longus.

93 us, we conclude that the type 1 pneumococcal pilus can activate cells via TLR2, and the ancillary pil

94 However, the pilus conductance and the carrier mobility decreased whe

95 The PI-2 pilus consists of repeated units of a single protein, Pi

96 on pilus post-translational modification and pilus density.

97 r motility and toxin production and promotes pilus-dependent biofilm formation, but no specific biolo

98 onal 11B9/61 antibody, which greatly reduced pilus-dependent cell contact.

99 olerae cells occurs in at least two steps: a pilus-dependent translocation of the incoming DNA across

100 ellum-driven motility and upregulate type IV pilus-dependent twitching motility of P. aeruginosa.

101 e deletion of the N3 ring leads to increased pilus dynamics, thereby compensating for the reduced num

102 binding is essential for the role of PilF in pilus dynamics.

103 are targeted for adherence by E. coli common pilus (ECP; or meningitis-associated and temperature-reg

104 tified by the presence of the bundle-forming pilus encoded by a virulence plasmid, which has been lin

105 Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally

106 fied a putative chemotaxis operon, a type IV pilus-encoding cluster and a region encoding putative ar

107 While FasX reduced pilus expression in each serotype, the molecular basis d

108 Heterologous pneumococcal pilus expression in Escherichia coli was obtained by rep

109 e capsule production proteins, regulators of pilus expression, and two-component gene regulatory syst

110 ng of PilMNOP were not essential for surface pilus expression, whereas the highly conserved inner mem

111 t the carrier mutation (liaS(R135G)) affects pilus expression.

112 In this article, a coarse-grained model of pilus extension and attachment is developed.

113 OMCC cap and a pilus tip protein coregulate pilus extension but are not required for channel assembl

114 ologically relevant conditions indicate that pilus extension is a quasistatic process such that the p

115 on between HmpF and the T4P system activates pilus extension, the Hmp system facilitates coordinated

116 lay essential roles: its formation may drive pilus extrusion by preventing backsliding of the nascent

117 d two periplasmic gates open to make way for pilus extrusion.

118 e PilQ protein, essential for DNA uptake and pilus extrusion.

119 2 infects Escherichia coli via the host 'sex pilus' (F-pilus); it was the first fully sequenced organ

120 A model of the G. sulfurreducens pilus fiber is proposed based on docking of this structu

121 However, incorporation of EbpA into the pilus fiber only required its sortase recognition motif

122 med for addition of the pilin to the nascent pilus fiber.

123 These subunits are held together in the pilus filament by hydrophobic interactions among their N

124 Here we identify the role of the F9/Yde/Fml pilus for UPEC persistence in the inflamed urothelium.

125 pilA1 (CD3513) and pilB1 (CD3512), abolished pilus formation and significantly reduced cell aggregati

126 e relationship between protein secretion and pilus formation in Vibrio cholerae.

127 l cells, and previous studies suggested that pilus formation is dependent on the presence of the cag

128 cterial type II secretion system and type IV pilus formation were shown to specifically bind c-di-GMP

129 dentify specific genes that are required for pilus formation, and the role of pili in T4SS function i

130 T4SS function but retained the capacity for pilus formation.

131 regained T4SS function and the capacity for pilus formation.

132 igated whether these genes were required for pilus formation.

133 rane proteins that are essential for type IV pilus formation.

134 nts were defective in both T4SS function and pilus formation; complemented mutants regained T4SS func

135 us assembly, and CofB can be detected in the pilus fraction.

136 bacterium diphtheriae and FimA of the type 2 pilus from Actinomyces oris unfold and extend at forces

137 s of the pilus subunit SpaA of the SpaA-type pilus from Corynebacterium diphtheriae and FimA of the t

138 A comprehensive model of the type 1 pilus from S. pneumoniae is also presented.

139 tron microscopy reconstruction of the intact pilus, from which we built an atomic model for the filam

140 By inactivating the retraction aspect of pilus function, genes essential for T4P assembly were di

141 type IV filaments is the gonococcal type IV pilus (GC-T4P) from Neisseria gonorrhoeae, the causative

142 e that some surface proteins not linked to a pilus gene cluster in Gram-positive bacteria may hijack

143 bly, the TG motif was identified in multiple pilus gene clusters of other Gram-positive bacteria, sug

144 Our data suggest new mechanisms of pilus gene regulation in GAS and that the invasiveness a

145 AS and that the invasiveness associated with pilus gene regulation in GAS differs from the enhanced i

146 Here, we present an atomic model of the P pilus generated from a 3.8 A resolution cryo-electron mi

147 CT) region of the genome, which contains the pilus genes (nine different FCT-types), and (c) the stre

148 but are required for increased expression of pilus genes upon surface association, indicating that th

149 , encoding the direct activator of toxin and pilus genes.

150 nd provide a rationale for the prevalence of pilus glycosylation in nature.

151 These systems fall into the type IVb pilus group, which can function in bacterial adhesion.

152 n ssRNA phage specific for the conjugative F-pilus, has a T = 3 icosahedral lattice of coat proteins

153 ata presented here confirm the importance of pilus I for S. pneumoniae pathogenesis and the potential

154 nd were able to demonstrate the role of Pil3 pilus in binding to colonic mucus.

155 rt the visualization of a competence-induced pilus in the Gram-negative bacterium Vibrio cholerae.

156 Elimination of pilus in the isogenic carrier mutant increased the level

157 altered transcription of the genes encoding pilus in the presence of bacitracin.

158 of a bona fide physiological role for a Tad pilus in this bacterial family.

159 The roles of the pilins and of the pilus in transformation are discussed.

160 incoming DNA across the outer membrane and a pilus-independent shuttling of the DNA through the perip

161 The type PI-1 streptococcal pilus is a complex, well studied structure, but the PI-2

162 EC translocation, suggesting that the type 1 pilus is a therapeutic target for the prevention of this

163 The Type IV pilus is also a strictly conserved trait in commensal Ne

164 e endocarditis- and biofilm-associated (Ebp) pilus is an important virulence factor for Enterococcus

165 The pneumococcal type 1 pilus is an inflammatory and adherence-promoting structu

166 This plaited pilus is encoded by the competence (com)G locus, and, af

167 the ComE pore through which the NTHI type IV pilus is expressed.

168 ypes Ib (P = .033) and V (P = .040); and for pilus island (PI)-1 (P = .016), PI-2a (P = .015), PI-2b

169 The Streptococcus pnuenomae pilus island 1 pili are composed of three subunits, RrgA

170 ing antiserum for GBS80 (backbone protein of pilus island-I), GBS67 (ancillary protein of PI-2a), and

171 Escherichia coli via the host 'sex pilus' (F-pilus); it was the first fully sequenced organism and is

172 and Pil proteins compose the JPC, a type IV pilus-like nanomotor that drives motility and polysaccha

173 e implies that the JPC is a modified type IV pilus-like structure encoded for in part by genes in the

174 d, the expression of a tight adherence (tad) pilus locus was elevated in NT.

175 yse is the Vibrio cholerae toxin-coregulated pilus machine (TCPM).

176 at assembles and retracts them (the type IVa pilus machine, or T4PM) in situ, in both the piliated an

177 nd inner membrane components of the type IVa pilus machinery in P. aeruginosa, with PilM binding to P

178 s, indicating that this is unique to type IV pilus-mediated secretion.

179 mutants were hyperpiliated but defective in pilus-mediated twitching motility.

180 fects in both flagella-mediated swimming and pilus-mediated twitching motility.

181 ents/min) are 15-times more frequent than in pilus-minus mutant cells (0.2 events/min), indicating th

182 was the same for wild-type cells (12 s) and pilus-minus mutant cells (13 s), suggesting the pili do

183 ity (eg, the pilT and galU genes involved in pilus motility and LPS formation).

184 In this study, we used the type IV pilus of Neisseria gonorrhoeae to test whether variation

185 essed the HIV-1 Gag-p24 on the tip of the T3 pilus of Streptococcus pyogenes as a fusion to the Cpa p

186 are located directly downstream of a type IV pilus operon in strongly cellulolytic members of the gen

187 nnose-sensitive hemagglutinin (MSHA) type IV pilus operon), had reduced infectivity of A. cytherea.

188 nt to colonic mucus is dependent on the pil3 pilus operon, which is heterogeneously expressed in the

189 e manipulation of surfaces and modulation of pilus-pilus interactions.

190 a low-energy pathway along which the nascent pilus polymer is guided during secretion.

191 Bacterial sorting was dependent on pilus post-translational modification and pilus density.

192 d aggregation phenotypes associated with Tad pilus production and efficient bacterial retention by fe

193 s biogenesis, a widespread assembly line for pilus production at the surface of Gram-negative bacteri

194 poly-Leu/Ala TM domain sequence also blocked pilus production but not substrate transfer or formation

195 anced invasiveness associated with increased pilus production in other bacterial pathogens.

196 Elimination of pilus production in the isogenic carrier mutant decrease

197 ocesses, DNA transfer, protein transfer, and pilus production, can be uncoupled and that the latter t

198 ated systems, the homologous protein acts in pilus production, mating pair stabilization, and entry e

199 trigger P. aeruginosa T6SS killing, but not pilus production.

200 tes to a carrier phenotype through increased pilus production.

201 For this purpose, we chose the pilus protein FimG from Gram-negative bacteria and a dis

202 supports the idea of making a more effective pilus protein-based vaccine that can be used universally

203 VII invasion and adherence was inhibited by pilus protein-specific antiserum SAN1518 significantly (

204 found that mutant strains, deficient in the pilus proteins (Deltagbs80 and Deltasan1518) exhibit a s

205 To check the role of pilus proteins in adherence and invasion, an inhibition

206 es to GBS capsular polysaccharides (CPS) and pilus proteins in European pregnant women.

207 accharides and anti-BP-1, -AP1-2a and -BP-2b pilus proteins were determined by enzyme-linked immunoso

208 Because the pilus proteins were identified previously as vaccine can

209 Antibody levels against CPS and pilus proteins were significantly higher in GBS colonize

210 Pilus-proteins were commonly found up-regulated, and the

211 becomes transiently open by secretion of the pilus, providing the entry port for exogenous DNA to gai

212 We show that the pilus regulatory gene fimK promotes the virulence of K.

213 The toxin-coregulated pilus (TCP), a type IV pilus required for V. cholerae pathogenesis, is necessar

214 type IV pili to a solid surface, followed by pilus retraction and signal transduction through the Chp

215 and type IV pilus retraction, we can exclude pilus retraction as a mechanism for DNA uptake.

216 diA-CT(536) interacts with F pilin, and that pilus retraction is critical for toxin import.

217 Based on force-dependent unbinding rates and pilus retraction speeds measured at the level of single

218 C. crescentus thus repurposes pilus retraction, typically used for surface motility, f

219 ity-force relation of DNA uptake and type IV pilus retraction, we can exclude pilus retraction as a m

220 For twitching, powered by type-IV pilus retraction, we find that individual cells exert lo

221 ysically blocking pili imposed resistance to pilus retraction, which was sufficient to stimulate hold

222 o bind F pili and become internalized during pilus retraction.

223 , we confirm memory in the form of bursts of pilus retractions.

224 tip fibrillum at the top, mounted on a long pilus rod.

225 lity reversals but is independent of type IV pilus "S motility." The inheritance of opposing polarity

226 nical properties and illuminates its role in pilus secretion.

227 n Ag on the tip of the group A Streptococcus pilus serves as an excellent vaccine platform to induce

228 Furthermore, the pilus shaft assembly in Gram-positive bacteria may requi

229 se unique tip proteins displayed on a common pilus shaft may serve distinct physiological functions.

230 Here we demonstrate that pneumococcal type I pilus significantly increases the adhesiveness of poorly

231 fications protect P. aeruginosa from certain pilus-specific phages.

232 RrgC does not depend on pilus-specific sortases to become attached to the cell w

233 nt discrepancies in the literature regarding pilus stiffness and the location of adhesins on pili.

234 transcription of pilE, the gene encoding the pilus structural subunit.

235 t the first helix is involved in forming the pilus structure core and that parts of helices two and t

236 rmation of a native morphologically distinct pilus structure on the bacterial surface.

237 This mutant also fails to assemble pilus structures and is greatly defective in toxin produ

238 o displays on its cell surface mucus-binding pilus structures, along with other LPXTG surface protein

239 ccus faecalis pili, EbpC, labels polymerized pilus structures, diminishes biofilm formation, and sign

240 lack or an alteration of the L. rhamnosus GG pilus structures, indicating that the TG motif is critic

241 ymerization, minor subunit organization, and pilus subcellular compartmentalization in the E. faecali

242 We show that RrgA, an ancillary pilus subunit devoid of a lipidation motif, particularly

243 n activate cells via TLR2, and the ancillary pilus subunit RrgA is a key component of this activation

244 pectroscopy, we find that these loops of the pilus subunit SpaA of the SpaA-type pilus from Corynebac

245 nts governing the incorporation of the three pilus subunits (EbpA, EbpB, and EbpC) have not been inve

246 h is displaced to allow the translocation of pilus subunits across the membrane.

247 the chaperone-usher pathway, are polymers of pilus subunits assembling into two parts: a thin, short

248 studied the contributions of the individual pilus subunits EmpA, EmpB, and EmpC to pilus architectur

249 nition motif and the pilin-like motif of the pilus subunits.

250 how it orchestrates the ordered assembly of pilus subunits.

251 the environment, is supported by the type IV pilus system in most species.

252 n orthologues of Type 2 secretion and Type 4 pilus system proteins.

253 compartmentalization in the E. faecalis Ebp pilus system.

254 o cholerae are among the simplest of Type IV pilus systems and possess only a single minor pilin.

255 ding filament growth in more complex Type IV pilus systems as well as the related Type II secretion s

256 Atu0216 to Atu0224), homologous to tad-type pilus systems from several bacteria, including Aggregati

257 The Type IV pilus systems of enterotoxigenic Escherichia coli and Vi

258 ce and the coordinate expression of multiple pilus systems.

259 s known that S motility requires the type IV pilus (T4P) and the exopolysaccharide (EPS) to function.

260 tant model system for the studies of Type IV pilus (T4P) because it is motile by social (S) motility

261 nner membrane proteins essential for type IV pilus (T4P) expression in Pseudomonas aeruginosa.

262 The bacterial type IV pilus (T4P) is a versatile molecular machine with a broa

263 investigated the role of the primary type IV pilus (T4P) locus in c-di-GMP-dependent cell aggregation

264 ity powered by the retraction of the type IV pilus (T4P).

265 o major virulence factors, toxin-coregulated pilus (TCP) and cholera toxin (CT).

266 ors cholera toxin (CT) and toxin-coregulated pilus (TCP) are capable of pandemic spread of cholera di

267 This GI contains the toxin-coregulated pilus (TCP) gene cluster that is necessary for colonizat

268 The toxin-coregulated pilus (TCP), a type IV pilus required for V. cholerae pa

269 ess cholera toxin (CT) and toxin-coregulated pilus (TCP), two main virulence factors required for dis

270 n of cholera toxin and the toxin-coregulated pilus (TCP).

271 major colonization factor, toxin coregulated pilus (TCP).

272 ctors: cholera toxin and a toxin-coregulated pilus (TCP).

273 ously, we have demonstrated that the type IV pilus (Tfp) of P. aeruginosa mediates resistance to anti

274 via the tip, but if not then the part of the pilus that attaches can be quite far from the tip.

275 e and for the assembly of the transformation pilus that mediates DNA transport.

276 The Bacteroidia pilus, therefore, has a biogenesis mechanism that is dis

277 thyretin-like repeats that polymerize into a pilus through a strand-exchange mechanism.

278 quired to prevent backsliding of the nascent pilus through the FimD pore and also reveals unexpected

279 Studying the pilus tip adhesin Spy0125 of Streptococcus pyogenes, we

280 Furthermore, the OMCC cap and a pilus tip protein coregulate pilus extension but are not

281 the gene encoding the mannose-binding type 1 pilus tip protein FimH demonstrated reduced binding and

282 f would be required for incorporation of the pilus tip subunit, while incorporation of the base subun

283 Cpa, the adhesin at the pilus tip, was recently shown to have a thioester-contai

284 ities varied when FimH was incorporated into pilus tip-like, FimCGH complexes.

285 target receptors and thus the streptococcal pilus to host cells.

286 e cell wall; instead, it binds the preformed pilus to the peptidoglycan by employing the catalytic ac

287 We further demonstrate that each pilus type binds preferentially to particular phospholip

288 tinct chaperone-usher pathway pili, and each pilus type may enable colonization of a habitat in the h

289 al that F17-like pili are closely related to pilus types carried by intestinal pathogens, but are res

290 ic isolates that lack one, two, or all three pilus types were also attenuated in virulence.

291 obtained in parallel and their capsular and pilus types were identified by serological and molecular

292 solates that were positive for toxin and all pilus types.

293 ntaining the PLUG in the TD channel of the P pilus usher PapC, and a loop between the 12th and 13th b

294 ressed pilin locus (pilE) allow for numerous pilus variants per strain to be produced from a single s

295 grown under conditions that activate the TCP pilus virulence regulatory protein (ToxT) virulence regu

296 he type II secretion system, and the type IV pilus, were dispensable for YbcL(UTI) release from UPEC.

297 assemble to form a secretion machinery and a pilus while the VirD4 protein is responsible for substra

298 ound that a bacterium tethered with a type 1 pilus will experience significantly reduced shear stress

299 n, and RrgC, which presumably associates the pilus with the bacterial cell wall.

300 reventing backsliding of the nascent growing pilus within the secretion pore; the rod also has striki