ライフサイエンスコーパス: pipet

1 pulling platinum (Pt) wires in a theta glass pipet.

2 vity enclosed in the terminal of a capillary pipet.

3 rofluidic structures by using a conventional pipet.

4 of a laser-pulled quartz quadruple-barrelled pipet.

5 bout 3 times the inner opening radius of the pipet.

6 to that of a manually spotted volume with a pipet.

7 and the complex in the outer solution of the pipets.

8 e configurations of traditional multichannel pipets.

9 gement is used for characterization of theta-pipets.

10 with 200 muL of 0.9% NaCl buffer directly by pipetting.

11 immediately dispersed into single cells upon pipetting.

12 g SLT-1B from complex cell lysates simply by pipetting 20 muL of the sample in and out of the tip in

13 hite powder or nanotubes in acetonitrile and pipeting a small volume onto the electrode surface and a

14 enotypes of E. coli can be assayed by merely pipetting a cell suspension into seven microplate arrays

15 , inner pipet) and aspirate (the sink, outer pipet) a buffer solution (perfusate) into each of the tw

16 ferent substrates such as the tip of a glass pipet, a double-barrel pipet, and a freestanding silicon

17 s down to approximately 100 nL, although the pipetting accuracy and precision deteriorate considerabl

18 bes comparative results between the use of a pipet and a microfluidic-based sampling device for the c

19 g inocula of 10(5) or 10(6) CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 McFarl

20 of a laser-pulled quartz quadruple-barreled pipet and filling the open channels with electrolyte sol

21 ng to the ingress of the common ion into the pipet and its egress into the external solution.

22 Analytical data from DBS prepared with a pipet and microfluidic-based sampling devices were compa

23 thrombin clots that are capable of plugging pipets and automated liquid-transfer systems.

24 table clamp, so on-chip operation only needs pipetting and adjusting of clamping force.

25 protocols are presented, one that minimizes pipetting and another that minimizes the number of react

26 Further, errors in repetitive pipetting and calculation are eliminated.

27 ple microwells thus avoiding repeated manual pipetting and costly robots.

28 ys may be performed with standard laboratory pipetting and detection systems.

29 Pipetting and dilution are universal processes used in c

30 ocular-surface epithelia can be isolated by pipetting and FACS sorting into a population of corneal

31 ability to dispense (i.e., the source, inner pipet) and aspirate (the sink, outer pipet) a buffer sol

32 as the tip of a glass pipet, a double-barrel pipet, and a freestanding silicon nitride membrane.

33 using a transfer pipet, docked to a holding pipet, and held firmly during physical agitation and int

34 re individually isolated in a microcapillary pipet, and the beta-cells were identified by amplificati

35 ed by a mixing cycle, is carried out with no pipeting, and a sample volume of 400 nL is sufficient fo

36 eater lateral distances than the size of the pipet aperture.

37 ly, when membranes contain Ni(2+) complexes, pipetting aqueous polyhistidine-tagged protein through t

38 p check valve, a diverter valve, and a 10-nL pipet are demonstrated.

39 other methods, issues related to incomplete pipetting are virtually eliminated.

40 We demonstrate a novel and versatile pipet-based approach to study the landing of individual

41 Overall, for the pipet-based deposition methods, results showed that the

42 CM), which is a technique that uses a mobile pipet-based electrochemical cell as an imaging probe.

43 For the two pipet-based sample deposition methods tested in this stu

44 , were compared in this study along with two pipet-based sample deposition methods, dried-droplet and

45 unis can be made accessible to a patch-clamp pipet by removing a small portion (1-3 micrometers in di

46 ced whereby the two carbon electrodes in the pipet can be used to promote redox cycling of an individ

47 ts involving the same NP captured inside the pipet cavity.

48 ed using a "quad-probe" (four-channel probe) pipet cell, fabricated by depositing carbon pyrolyticall

49 xternal solution and collected at the second pipet ("collector"), (2) measurements of ohmic current-v

50 Using our pipet, complex paper-based microfluidic devices can be f

51 d the feasibility of automating the specimen-pipetting component of sample preparation in the LCx Chl

52 positioned above the nanopore as a change in pipet current.

53 the concentration of chemoattractant in the pipet delivering chemoattractant.

54 A simple pipetting device was developed for reliable application

55 into sample reservoirs by using an eight-tip pipetting device, and the chip is docked with an array o

56 e of the small electrode area defined by the pipet dimensions, the background current is low, allowin

57 solution (<2 muL) and the MNPs (1-3 mug) by pipetting directly onto a matrix-assisted laser desorpti

58 rted to a dye-free solution using a transfer pipet, docked to a holding pipet, and held firmly during

59 After the pipet dosing was characterized in detail, two proof-of-c

60 ipets (subsequently called "dual superfusion pipet"), each providing the ability to dispense (i.e., t

61 positively charged heparin ionophores using pipet electrodes filled with the organic electrolyte sol

62 Novel dual-pipet electrodes prepared by pulling borosilicate theta-

63 Water-filled pipet electrodes were also used to study the stoichiomet

64 While linear multichannel pipets enable many established assays, they do not suppo

65 f electrolyte protruding from the tip of the pipet ensures that a gentle meniscus contact is made wit

66 r step causing inaccurate pipetting or total pipetting failure.

67 technique in which the probe is a fine glass pipet filled with a contact (reference) electrode and an

68 complexation reactions, with a multichannel pipet for adding the reagent and an infrared camera to m

69 combination with a programmable multichannel pipet form a highly efficient epitope mapping system.

70 range fluidic/electrical connections so that pipetting functions can be performed conveniently.

71 s waves are required for characterization of pipet geometry and precise determination of thermodynami

72 It is found that the pipet geometry plays an important role in controlling th

73 s slow diffusion of the DNA molecules in the pipet has also been observed.

74 Here, we develop a hand-held single-cell pipet (hSCP) that is rapid, operationally simple, highly

75 ance to that of a calibrated, single-channel pipet in volume delivery experiments.

76 ter mix, and detection reagents with minimal pipetting, in a hand-held, disposable device intended fo

77 including plasma and urine, are deposited by pipet into a bed of CuO powder that fills a space within

78 m chloride, 1 mL of acetonitrile extract was pipetted into a 2-mL centrifuge tube containing anhydrou

79 aliquots of batch-cultured cells are rapidly pipetted into a hypotonic medium.

80 Samples are pipetted into an array of separable, multiplexed affinit

81 lacement or 150 mM mannitol or sucrose, were pipetted into cortical cups 35 min prior to ischemia and

82 (8 x 10(4)) and 3H-thymidine in medium were pipetted into each well to cover the treated and control

83 s), in the end of a tapered micrometer-scale pipet is described.

84 study, because the meniscus of the scanning pipet is only in contact with the surface investigated f

85 A patch clamp pipet is used to withdraw approximately 3 pL of cytoplas

86 faces surrounded by a thin glass wall of the pipets is enhanced by diffusion of the species from behi

87 The conical geometry of the pipet leads to a localized electric field, since all of

88 Samples are not pipetted manually but deposited by dragging one or sever

89 sily completed by either manual or automated pipetting methods.

90 d any blocking product is left behind as the pipet moves to a new location.

91 oss numerous 96 well plates and guidance for pipetting, non-trivial tasks for which informatics and v

92 ereafter "chicken matrix"), with just single pipetting of sample (i.e., no filtration, culturing and/

93 sample processing is required apart from the pipetting of the sample into a tin foil cup, which is pl

94 ts exerted by the charged inner surface of a pipet on IT processes.

95 EMD ranging from 15 to 125 ng/5 microl were pipetted onto a 3-mm diameter x 2-mm thick bioabsorbable

96 Equal cell samples were pipetted onto calcium phosphate-coated disks in 24-well

97 For loading, the suspended microcrystals are pipetted onto the chip and excess mother liquor is subse

98 estimates the sample height by up to several pipet opening radii.

99 omies of scale in reducing the complexity of pipetting operations.

100 dy-state counterpart obtained with a smaller pipet or at a slower scan rate.

101 A multichannel pipet or automated liquid-transfer system is used to tra

102 time-consuming motions with a single-channel pipet or device designs limited to the configurations of

103 ated sample-transfer step causing inaccurate pipetting or total pipetting failure.

104 as to promote or restrict migration from the pipet orifice.

105 es, one fluorescent reader, and no automated pipetting, over one thousand genotypes can be generated

106 ables DNA amplification with only the use of pipets, pipet tips, and a heater.

107 e technique that employs a dual-barrel theta pipet probe containing electrolyte solution and quasi-re

108 tacted by electrolyte is of the order of the pipet probe dimensions.

109 ork we use a "hopping mode", where the SECCM pipet probe is translated toward the surface at a series

110 evice without loss of tumor cells during the pipetting process.

111 If one wishes to pipet reagents of less than 100 nL, new pipettors need t

112 Media and drugs are supplied by a pipetting robot system.

113 To close this gap, we developed Lego-based pipetting robots that reliably handle liquid volumes fro

114 low-cost household consumables, programming pipetting routines, and modifying robot designs, we enab

115 herichia coli and Salmonella within a single-pipetted sample.

116 With manual pipetting, setting up a plate takes about 2 h, the initi

117 conducted at eight different combinations of pipet size and pressure.

118 osity) were unable to explain the effects of pipet size on the general aspiration rate.

119 Finally, pipetting speed was significantly improved by using the

120 to be better than that obtained with manual pipet spotting.

121 mical gradients in culture, including puffer pipet spritzing and photolytic release of caged effector

122 ssay reagents, and automated using automated pipetting stations to increase the assay throughput.

123 highly sensitive, and requires only a single pipetting step.

124 erformed at the start of the assay (i.e., no pipetting steps are necessary during the assay).

125 lified, one-day LM-PCR protocol in which all pipetting steps can be performed by a robotic workstatio

126 ny recent techniques still involve laborious pipetting steps for spheroid manipulation such as collec

127 technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pip

128 voirs situated on the microchip in only five pipetting steps using an 8-channel pipettor.

129 distinct PCR reactions with only 2N + 1 = 41 pipetting steps, compared with the 3N2 = 1200 steps requ

130 less sample and an order of magnitude fewer pipetting steps.

131 trolled using a pair of concentric automated pipets (subsequently called "dual superfusion pipet"), e

132 e steady-state voltammograms of either IT at pipet-supported ITIES or electron transfer at solid elec

133 Steady-state voltammetry at the pipet-supported liquid/liquid interface has previously b

134 Our reconfigurable pipet supports the advancement of custom analytical tool

135 the pipet used, the applied voltage, and the pipet-surface separation.

136 methods require repetitive wash steps with a pipet that are laborious and time-consuming, making the

137 g steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are el

138 pepsin at known constant concentration were pipetted through an array of miniaturized chromatographi

139 d resilient; they self-heal when liquids are pipetted through them.

140 tion was achieved by scanning a 17 nm radius pipet tip at a distance as low as 1.3 nm from a highly p

141 mized through choice of appropriate affinity pipet tip derivatization chemistries.

142 a protease-modified membrane at the end of a pipet tip digests proteins in 30 s or less, and the shor

143 a nondestructive method for calibrating the pipet tip geometry by fitting the numerical model to the

144 droplet that is hanging from a (conductive) pipet tip in the organic filter phase.

145 Antibody digestion at the end of a pipet tip leads to 100% peptide coverage in MS analyses.

146 wo vesicles or to a vesicle on one end and a pipet tip on the other.

147 thods are applicable, including the use of a pipet tip or glass melting point tube, allowing integrat

148 x is excited by focusing a laser beam at the pipet tip to produce a submicrometer light source.

149 n, sol-gel materials were placed in a 10 muL pipet tip with glass wool on either side.

150 of ARPE-19 cells using a sequence-comb or a pipet tip, and it was allowed to heal in the presence or

151 into them using a syringe with a needle or a pipet tip, and then sealing the hole with nail hardener.

152 m weanling mice by oral wash using a plastic pipet tip, contains enough oral epithelial cells and lym

153 mples in a silica capillary, in a disposable pipet tip, in a polymer microchannel, or from samples de

154 magnetic plate with a stirrer, tubing, and a pipet tip, this easy-to-use device rivals the output of

155 c field guides these NA-PNA-beads toward the pipet tip, which they obstruct, leading to an indefinite

156 IB) milling to precisely expose a UME at the pipet tip.

157 ding interface, and a nanoliter-to-picoliter pipet tip.

158 band at the very end of the interior of the pipet tip.

159 by diffusion of the species from behind the pipet tip.

160 nges during quasi-controlled breakage of the pipet tip.

161 subpicomole levels using this novel lab-in-a-pipet-tip approach.

162 mpler platform utilizing low cost disposable pipet tips and conventional disposable microtiter well p

163 These materials are packed in pipet tips and in combination with a programmable multic

164 fractions from 75-microm nanoLC columns into pipet tips at a user-defined, timed interval, typically

165 method involves sequentially moving rows of pipet tips containing sample solutions in close proximit

166 These clots plug the pipet tips during a manual or automated sample-transfer

167 he nanoLC fractions could be archived in the pipet tips for analysis at a later date.

168 ntial for combining functional membranes and pipet tips for rapid sample purification and pretreatmen

169 The hydrazide pipet tips have great potential in achieving automation

170 tions are then reconstituted directly in the pipet tips in approximately 250 nL of solvent prior to a

171 equipped with antibody-derivatized affinity pipet tips was used for the parallel extraction of speci

172 arge diameter Pt and sealed in polypropylene pipet tips were employed for end-column detection in CE.

173 A amplification with only the use of pipets, pipet tips, and a heater.

174 izing antibodies immobilized within affinity pipet tips, and eluted onto enzymatically active mass sp

175 mon laboratory materials, such as disposable pipet tips, filter paper, tooth picks, and nylon mesh.

176 rm parallel protein elution from 96 affinity pipet tips, proper buffering for on-target digestion, te

177 d of purification with reversed-phase-packed pipet tips.

178 thods that integrate membrane technology and pipet tips.

179 V) was injected into a micrometer-size glass pipet to construct an Ag+-selective SECM tip.

180 croscopy (P-SICM), that utilizes a nanoscale pipet to differentiate paracellular and transcellular tr

181 e-molecule unfolding by dosing acid from the pipet to locally melt individual molecules of duplex DNA

182 In this study, we use a nanoscale pipet to precisely locate tTJs within epithelia and meas

183 For this purpose, calcium was pipetted to graphite furnace together with samples.

184 es multiple centrifugation steps and careful pipetting to avoid loss of the bead-bound complexes.

185 The method involves using hand pipetting to create an array of cell-laden nanoliter-siz

186 0 microm and depended on the diameter of the pipet used, the applied voltage, and the pipet-surface s

187 his tool by modifying a commercial 8-channel pipet using machined and 3D-printed components.

188 ork, Na(+) or OH(-) ions were dosed from the pipet using pulsed voltage-driven delivery.

189 ameters of rapid IT processes extracted from pipet voltammograms.

190 ntribution decreases and other effects, like pipet volume uncertainty (typically >3%), dominate.

191 moderate-to-large copy number (N0 > 10(2)), pipet volume uncertainty and variability in the amplific

192 ed methodology for silanization of the outer pipet wall to investigate the kinetics of the rapid tran

193 that is initially present outside a tapered pipet was simulated by the finite element method, enabli

194 were displaced from the captured NA without pipetting wash buffers or use of external force and equi

195 around the cell, additional dual superfusion pipets were inserted into the microchannel for defined t

196 These pipets were shown to exhibit exceptional stability in ca

197 ys are performed manually using constriction pipets, which greatly limits the utility of this system.

198 mbryonic mice in vivo and study, using patch-pipet whole-cell current and voltage clamp techniques, s

199 ve developed a multichannel air displacement pipet with reconfigurable channels for nonstandard liqui

200 des have been prepared by masking PPC coated pipets with an insulating parylene C film.

201 be the fabrication of nanoengineered holding pipets with concave seating surfaces and fine pressure c

202 t-distance data and verify this method using pipets with opening radii between 30 and 300 nm.

203 id bilayers and a polycarbonate sample using pipets with opening radii down to 8 nm.

204 cated rapidly from laser pulled theta quartz pipets, with the pH electrode prepared by in situ carbon

205 -well microtiter plate and automated using a pipeting workstation with performance suitable for high-

206 able, because variation in the amount of DNA pipetted would no longer contribute to variation in T/S,